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TY - JOUR AU - Terada, Rie AU - Urawa, Hiroko AU - Inagaki, Yoshishige AU - Tsugane, Kazuo AU - Iida, Shigeru PY - 2002 DA - 2002/10/01 TI - Efficient gene targeting by homologous recombination in rice JO - Nature Biotechnology SP - 1030 EP - 1034 VL - 20 IS - 10 AB - Modification of genes through homologous recombination, termed gene targeting, is the most direct method to characterize gene function. In higher plants, however, the method is far from a common practice. Here we describe an efficient and reproducible procedure with a strong positive/negative selection for gene targeting in rice, which feeds more than half of the world's population and is an important model plant. About 1% of selected calli and their regenerated fertile plants were heterozygous at the targeted locus, and only one copy of the selective marker used was found at the targeted site in their genomes. The procedure's applicability to other genes will make it feasible to obtain various gene-targeted lines of rice. SN - 1546-1696 UR - https://doi.org/10.1038/nbt737 DO - 10.1038/nbt737 ID - Terada2002 ER -