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TY - JOUR AU - Sedelnikova, Olga A. AU - Horikawa, Izumi AU - Zimonjic, Drazen B. AU - Popescu, Nicholas C. AU - Bonner, William M. AU - Barrett, J. Carl PY - 2004 DA - 2004/02/01 TI - Senescing human cells and ageing mice accumulate DNA lesions with unrepairable double-strand breaks JO - Nature Cell Biology SP - 168 EP - 170 VL - 6 IS - 2 AB - Humans and animals undergo ageing, and although their primary cells undergo cellular senescence in culture, the relationship between these two processes is unclear1,2. Here we show that γ-H2AX foci (γ-foci), which reveal DNA double-strand breaks (DSBs)3,4, accumulate in senescing human cell cultures and in ageing mice. They colocalize with DSB repair factors, but not significantly with telomeres. These cryptogenic γ-foci remain after repair of radiation-induced γ-foci, suggesting that they may represent DNA lesions with unrepairable DSBs. Thus, we conclude that accumulation of unrepairable DSBs may have a causal role in mammalian ageing. SN - 1476-4679 UR - https://doi.org/10.1038/ncb1095 DO - 10.1038/ncb1095 ID - Sedelnikova2004 ER -