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TY - JOUR AU - Lukas, Claudia AU - Falck, Jacob AU - Bartkova, Jirina AU - Bartek, Jiri AU - Lukas, Jiri PY - 2003 DA - 2003/03/01 TI - Distinct spatiotemporal dynamics of mammalian checkpoint regulators induced by DNA damage JO - Nature Cell Biology SP - 255 EP - 260 VL - 5 IS - 3 AB - Cell cycle checkpoints are signal transduction pathways activated after DNA damage to protect genomic integrity1. Dynamic spatiotemporal coordination is a vital, but poorly understood aspect, of these checkpoints. Here, we provide evidence for a strikingly different behaviour of Chk2 versus Nbs1, key mediators of the ataxia-telangiecatesia-mutated (ATM)-controlled checkpoint pathways induced by DNA double-strand breaks (DSBs)1,2. In live human cells with DSBs restricted to small sub-nuclear areas, Nbs1 was rapidly recruited to the damaged regions and underwent a dynamic exchange in the close vicinity of the DSB sites. In contrast, Chk2 continued to rapidly move throughout the entire nucleus, irrespective of DNA damage and including the DSB-free areas. Although phosphorylation of Chk2 by ATM occurred exclusively at the DSB sites, forced immobilization of Chk2 to spatially restricted, DSB-containing nuclear areas impaired its stimulating effect on p53-dependent transcription. These results unravel a dynamic nature of Nbs1 interaction with DSB lesions and identify Chk2 as a candidate transmitter of the checkpoint signal, allowing for a coordinated pan-nuclear response to focal DNA damage. SN - 1476-4679 UR - https://doi.org/10.1038/ncb945 DO - 10.1038/ncb945 ID - Lukas2003 ER -