(original) (raw)

TY - JOUR AU - Yoshimi, Kazuto AU - Kunihiro, Yayoi AU - Kaneko, Takehito AU - Nagahora, Hitoshi AU - Voigt, Birger AU - Mashimo, Tomoji PY - 2016 DA - 2016/01/20 TI - ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes JO - Nature Communications SP - 10431 VL - 7 IS - 1 AB - The CRISPR-Cas system is a powerful tool for generating genetically modified animals; however, targeted knock-in (KI) via homologous recombination remains difficult in zygotes. Here we show efficient gene KI in rats by combining CRISPR-Cas with single-stranded oligodeoxynucleotides (ssODNs). First, a 1-kb ssODN co-injected with guide RNA (gRNA) and Cas9 messenger RNA produce GFP-KI at the rat Thy1 locus. Then, two gRNAs with two 80-bp ssODNs direct efficient integration of a 5.5-kb CAG-GFP vector into the Rosa26 locus via ssODN-mediated end joining. This protocol also achieves KI of a 200-kb BAC containing the human SIRPA locus, concomitantly knocking out the rat Sirpa gene. Finally, three gRNAs and two ssODNs replace 58-kb of the rat Cyp2d cluster with a 6.2-kb human CYP2D6 gene. These ssODN-mediated KI protocols can be applied to any target site with any donor vector without the need to construct homology arms, thus simplifying genome engineering in living organisms. SN - 2041-1723 UR - https://doi.org/10.1038/ncomms10431 DO - 10.1038/ncomms10431 ID - Yoshimi2016 ER -