(original) (raw)

TY - JOUR AU - Beliveau, Brian J. AU - Boettiger, Alistair N. AU - Avendaño, Maier S. AU - Jungmann, Ralf AU - McCole, Ruth B. AU - Joyce, Eric F. AU - Kim-Kiselak, Caroline AU - Bantignies, Frédéric AU - Fonseka, Chamith Y. AU - Erceg, Jelena AU - Hannan, Mohammed A. AU - Hoang, Hien G. AU - Colognori, David AU - Lee, Jeannie T. AU - Shih, William M. AU - Yin, Peng AU - Zhuang, Xiaowei AU - Wu, Chao-ting PY - 2015 DA - 2015/05/12 TI - Single-molecule super-resolution imaging of chromosomes and in situ haplotype visualization using Oligopaint FISH probes JO - Nature Communications SP - 7147 VL - 6 IS - 1 AB - Fluorescence in situ hybridization (FISH) is a powerful single-cell technique for studying nuclear structure and organization. Here we report two advances in FISH-based imaging. We first describe the in situ visualization of single-copy regions of the genome using two single-molecule super-resolution methodologies. We then introduce a robust and reliable system that harnesses single-nucleotide polymorphisms (SNPs) to visually distinguish the maternal and paternal homologous chromosomes in mammalian and insect systems. Both of these new technologies are enabled by renewable, bioinformatically designed, oligonucleotide-based Oligopaint probes, which we augment with a strategy that uses secondary oligonucleotides (oligos) to produce and enhance fluorescent signals. These advances should substantially expand the capability to query parent-of-origin-specific chromosome positioning and gene expression on a cell-by-cell basis. SN - 2041-1723 UR - https://doi.org/10.1038/ncomms8147 DO - 10.1038/ncomms8147 ID - Beliveau2015 ER -