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TY - JOUR AU - Firulli, Anthony B. AU - McFadden, David G. AU - Lin, Qing AU - Srivastava, Deepak AU - Olson, Eric N. PY - 1998 DA - 1998/03/01 TI - Heart and extra-embryonic mesodermal defects in mouse embryos lacking the bHLH transcription factor Hand1 JO - Nature Genetics SP - 266 EP - 270 VL - 18 IS - 3 AB - The basic helix-loop-helix (bHLH) transcription factors, Handl and Hand2 (refs 1,2), also called eHand/Hxt/Thing1 and dHand/Hed/Thing2 (refs 3,4), respectively, are expressed in the heart and certain neural-crest derivatives during embryogenesis. In addition, Handl is expressed in extraembryonic membranes, whereas Hand2 is expressed in the deciduum. Previous studies have demonstrated that Hand2 is required for formation of the right ventricle of the heart and the aortic arch arteries5. We have generated a germline mutation in the mouse Handl gene by replacing the first coding exon with a β-galactosidase reporter gene. Embryos homozygous for the Hand1 null allele died between embryonic days 8.5 and 9.5 and exhibited yolk sac abnormalities due to a deficiency in extraembryonic mesoderm. Heart development was also perturbed and did not progress beyond the cardiac-looping stage. Our results demonstrate important roles for Handl in extraembryonic mesodermal and heart development. SN - 1546-1718 UR - https://doi.org/10.1038/ng0398-266 DO - 10.1038/ng0398-266 ID - Firulli1998 ER -