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TY - JOUR AU - Yu, Wenqiang AU - Ginjala, Vasudeva AU - Pant, Vinod AU - Chernukhin, Igor AU - Whitehead, Joanne AU - Docquier, France AU - Farrar, Dawn AU - Tavoosidana, Gholamreza AU - Mukhopadhyay, Rituparna AU - Kanduri, Chandrasekhar AU - Oshimura, Mitsuo AU - Feinberg, Andrew P AU - Lobanenkov, Victor AU - Klenova, Elena AU - Ohlsson, Rolf PY - 2004 DA - 2004/10/01 TI - Poly(ADP-ribosyl)ation regulates CTCF-dependent chromatin insulation JO - Nature Genetics SP - 1105 EP - 1110 VL - 36 IS - 10 AB - Chromatin insulators demarcate expression domains by blocking the cis effects of enhancers or silencers in a position-dependent manner1,2. We show that the chromatin insulator protein CTCF carries a post-translational modification: poly(ADP-ribosyl)ation. Chromatin immunoprecipitation analysis showed that a poly(ADP-ribosyl)ation mark, which exclusively segregates with the maternal allele of the insulator domain in the H19 imprinting control region, requires the bases that are essential for interaction with CTCF3. Chromatin immunoprecipitation–on–chip analysis documented that the link between CTCF and poly(ADP-ribosyl)ation extended to more than 140 mouse CTCF target sites. An insulator trap assay showed that the insulator function of most of these CTCF target sites is sensitive to 3-aminobenzamide, an inhibitor of poly(ADP-ribose) polymerase activity. We suggest that poly(ADP-ribosyl)ation imparts chromatin insulator properties to CTCF at both imprinted and nonimprinted loci, which has implications for the regulation of expression domains and their demise in pathological lesions. SN - 1546-1718 UR - https://doi.org/10.1038/ng1426 DO - 10.1038/ng1426 ID - Yu2004 ER -