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TY - JOUR AU - Ostersehlt, Lynn M. AU - Jans, Daniel C. AU - Wittek, Anna AU - Keller-Findeisen, Jan AU - Inamdar, Kaushik AU - Sahl, Steffen J. AU - Hell, Stefan W. AU - Jakobs, Stefan PY - 2022 DA - 2022/09/01 TI - DNA-PAINT MINFLUX nanoscopy JO - Nature Methods SP - 1072 EP - 1075 VL - 19 IS - 9 AB - MINimal fluorescence photon FLUXes (MINFLUX) nanoscopy, providing photon-efficient fluorophore localizations, has brought about three-dimensional resolution at nanometer scales. However, by using an intrinsic on–off switching process for single fluorophore separation, initial MINFLUX implementations have been limited to two color channels. Here we show that MINFLUX can be effectively combined with sequentially multiplexed DNA-based labeling (DNA-PAINT), expanding MINFLUX nanoscopy to multiple molecular targets. Our method is exemplified with three-color recordings of mitochondria in human cells. SN - 1548-7105 UR - https://doi.org/10.1038/s41592-022-01577-1 DO - 10.1038/s41592-022-01577-1 ID - Ostersehlt2022 ER -