(original) (raw)

TY - JOUR AU - Tarsounas, Madalena AU - Davies, Derek AU - West, Stephen C PY - 2003 DA - 2003/02/01 TI - BRCA2-dependent and independent formation of RAD51 nuclear foci JO - Oncogene SP - 1115 EP - 1123 VL - 22 IS - 8 AB - The formation of RAD51 foci in response to ionizing radiation (IR) represents an important step in the repair of DNA double-strand breaks. RAD51 foci also appear during S phase and are thought to be required for the restart of stalled or broken replication forks. The RAD51 recombinase interacts directly with the breast cancer-associated tumour suppressor BRCA2, an interaction that is required for normal recombination proficiency, radiation resistance and genome stability. In CAPAN-1 cells, which express a truncated form of BRCA2 that is cytoplasmic because of loss of the nuclear localization signal, the formation of IR-induced RAD51 foci is impaired. In this work, we show that S-phase RAD51 foci form normally in CAPAN-1 cells expressing truncated BRCA2. Moreover, we find that RAD51 specifically associates with chromatin at S phase in a reaction that is BRCA2-independent. The observed BRCA2-dependent and independent formation of RAD51 foci shows that intact BRCA2 is not required for RAD51 focus formation per se, leading us to suggest that S phase and IR-induced RAD51 foci assemble by distinct pathways with defined protein requirements. SN - 1476-5594 UR - https://doi.org/10.1038/sj.onc.1206263 DO - 10.1038/sj.onc.1206263 ID - Tarsounas2003 ER -