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TY - JOUR AU - Lin, Xiaosi AU - Yang, Ting AU - Wang, Shicong AU - Wang, Zhen AU - Yun, Ye AU - Sun, Lixiang AU - Zhou, Yunhe AU - Xu, Xiaohui AU - Akazawa, Chihiro AU - Hong, Wanjin AU - Wang, Tuanlao PY - 2014 DA - 2014/12/02 TI - RILP interacts with HOPS complex via VPS41 subunit to regulate endocytic trafficking JO - Scientific Reports SP - 7282 VL - 4 IS - 1 AB - The HOPS complex serves as a tethering complex with GEF activity for Ypt7p in yeast to regulate late endosomal membrane maturation. While the role of HOPS complex is well established in yeast cells, its functional and mechanistic aspects in mammalian cells are less well defined. In this study, we report that RILP, a downstream effector of Rab7, interacts with HOPS complex and recruits HOPS subunits to the late endosomal compartment. Structurally, the amino-terminal portion of RILP interacts with HOPS complex. Unexpectedly, this interaction is independent of Rab7. VPS41 subunit of HOPS complex was defined to be the major partner for interacting with RILP. The carboxyl-terminal region of VPS41 was mapped to be responsible for the interaction. Functionally, either depletion of VPS41 by shRNA or overexpression of VPS41 C-terminal half retarded EGF-induced degradation of EGFR. These results suggest that interaction of RILP with HOPS complex via VPS41 plays a role in endocytic trafficking of EGFR. SN - 2045-2322 UR - https://doi.org/10.1038/srep07282 DO - 10.1038/srep07282 ID - Lin2014 ER -