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TY - JOUR AU - Moccia, Maria Domenica AU - Oger-Desfeux, Christine AU - Marais, Gabriel AB AU - Widmer, Alex PY - 2009 DA - 2009/05/25 TI - A White Campion (Silene latifolia) floral expressed sequence tag (EST) library: annotation, EST-SSR characterization, transferability, and utility for comparative mapping JO - BMC Genomics SP - 243 VL - 10 IS - 1 AB - Expressed sequence tag (EST) databases represent a valuable resource for the identification of genes in organisms with uncharacterized genomes and for development of molecular markers. One class of markers derived from EST sequences are simple sequence repeat (SSR) markers, also known as EST-SSRs. These are useful in plant genetic and evolutionary studies because they are located in transcribed genes and a putative function can often be inferred from homology searches. Another important feature of EST-SSR markers is their expected high level of transferability to related species that makes them very promising for comparative mapping. In the present study we constructed a normalized EST library from floral tissue of Silene latifolia with the aim to identify expressed genes and to develop polymorphic molecular markers. SN - 1471-2164 UR - https://doi.org/10.1186/1471-2164-10-243 DO - 10.1186/1471-2164-10-243 ID - Moccia2009 ER -