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TY - JOUR AU - Kleene, Kenneth C. AU - Bagarova, Jana AU - Hawthorne, Sabrina K. AU - Catado, Leah M. PY - 2010 DA - 2010/12/25 TI - Quantitative analysis of mRNA translation in mammalian spermatogenic cells with sucrose and Nycodenz gradients JO - Reproductive Biology and Endocrinology SP - 155 VL - 8 IS - 1 AB - Developmental and global regulation of mRNA translation plays a major role in regulating gene expression in mammalian spermatogenic cells. Sucrose gradients are widely used to analyze mRNA translation. Unfortunately, the information from sucrose gradient experiments is often compromised by the absence of quantification and absorbance tracings, and confusion about the basic properties of sucrose gradients. SN - 1477-7827 UR - https://doi.org/10.1186/1477-7827-8-155 DO - 10.1186/1477-7827-8-155 ID - Kleene2010 ER -