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TY - JOUR AU - Fisher, Sheila AU - Barry, Andrew AU - Abreu, Justin AU - Minie, Brian AU - Nolan, Jillian AU - Delorey, Toni M. AU - Young, Geneva AU - Fennell, Timothy J. AU - Allen, Alexander AU - Ambrogio, Lauren AU - Berlin, Aaron M. AU - Blumenstiel, Brendan AU - Cibulskis, Kristian AU - Friedrich, Dennis AU - Johnson, Ryan AU - Juhn, Frank AU - Reilly, Brian AU - Shammas, Ramy AU - Stalker, John AU - Sykes, Sean M. AU - Thompson, Jon AU - Walsh, John AU - Zimmer, Andrew AU - Zwirko, Zac AU - Gabriel, Stacey AU - Nicol, Robert AU - Nusbaum, Chad PY - 2011 DA - 2011/01/04 TI - A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries JO - Genome Biology SP - R1 VL - 12 IS - 1 AB - Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol. SN - 1474-760X UR - https://doi.org/10.1186/gb-2011-12-1-r1 DO - 10.1186/gb-2011-12-1-r1 ID - Fisher2011 ER -