Christina Wells | Clemson University (original) (raw)
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Papers by Christina Wells
Http Dx Doi Org 10 1080 01904160903470497, Jan 8, 2010
The relationship between root age and root physiology is poorly understood, despite its importanc... more The relationship between root age and root physiology is poorly understood, despite its importance for nutrient absorption. In peaches, roots are white when they first appear and then become brown with age, which corresponds to a number of physiological changes. We related root ...
PLOS ONE, 2015
Low-cost, high throughput genotyping methods are crucial to marker discovery and marker-assisted ... more Low-cost, high throughput genotyping methods are crucial to marker discovery and marker-assisted breeding efforts, but have not been available for many 'specialty crops' such as fruit and nut trees. Here we apply the Genotyping-By-Sequencing (GBS) method developed for cereals to the discovery of single nucleotide polymorphisms (SNPs) in a peach F2 mapping population. Peach is a genetic and genomic model within the Rosaceae and will provide a template for the use of this method with other members of this family. Our F2 mapping population of 57 genotypes segregates for bloom time (BD) and chilling requirement (CR) and we have extensively phenotyped this population. The population derives from a selfed F1 progeny of a cross between 'Hakuho' (high CR) and 'UFGold' (low CR). We were able to successfully employ GBS and the TASSEL GBS pipeline without modification of the original methodology using the ApeKI restriction enzyme and multiplexing at an equivalent of 96 samples per Illumina HiSeq 2000 lane. We obtained hundreds of SNP markers which were then used to construct a genetic linkage map and identify quantitative trait loci (QTL) for BD and CR.
VI International Peach Symposium, 2006
The foliar nematode Aphelenchoides fragariae (Aphelenchida: Aphelenchidae) is an endo- and ectopa... more The foliar nematode Aphelenchoides fragariae (Aphelenchida: Aphelenchidae) is an endo- and ectoparasite that feeds on aerial parts of over 250 plant species from 47 families. It is a common and economically-damaging pest of nursery-grown crops including ferns, foliage and flowering plants, and herbaceous and woody perennials. We used Illumina HiSeq 2000 technology and the Trinity de novo assembler (trinityrnaseq.sourceforge.net/) to produce a transcriptome of A. fragariae from four growth conditions (fungal-fed, plant-fed, diet-changed, and desiccated). High throughput sequencing generated 43 million reads with average length of 100-bp. Assembly of the pooled read set generated 95,930 contigs, corresponding to 50,686 unigenes (mean length = 605 bp) with 45,244 alternate splice variants. Fifty-two percent of the unigenes (26,389) had homology to known genes or proteins in the NCBI non-redundant database, and 20,558 were assigned GO terms based on these homologies by Blast2Go software...
Journal of Plant Nutrition, 2010
The relationship between root age and root physiology is poorly understood, despite its importanc... more The relationship between root age and root physiology is poorly understood, despite its importance for nutrient absorption. In peaches, roots are white when they first appear and then become brown with age, which corresponds to a number of physiological changes. We related root ...
Douglas G. Bielenberg: A.
... March 2009 Performance of Mycorrhizal Products Marketed for Woody Landscape Plants1 P. Eric W... more ... March 2009 Performance of Mycorrhizal Products Marketed for Woody Landscape Plants1 P. Eric Wiseman2, Kristen H. Colvin3, and Christina E. Wells4 Department of Horticulture, Clemson University, Clemson, SC 29634 Abstract ... 65.9 g/L 1, 2, 3, 5 Santa Maria, CA and one ...
Page 1. 70 J. Environ. Hort. 27(2):70–79. June 2009 Arbuscular Mycorrhizal Inoculation Affects Ro... more Page 1. 70 J. Environ. Hort. 27(2):70–79. June 2009 Arbuscular Mycorrhizal Inoculation Affects Root Development of Acer and Magnolia Species1 P. Eric Wiseman2 and Christina E. Wells3 Department of Forestry, Virginia Tech ...
Machine Vision and Applications
Journal of nematology
Desiccation tolerance plays an important role in the overwinter survival of the foliar nematode A... more Desiccation tolerance plays an important role in the overwinter survival of the foliar nematode Aphelenchoides fragariae. Survival rates of A. fragariae were compared with those of the anhydrobiotic soil-dwelling nematode Aphelenchus avenae after desiccation (90% RH), cold (4°C) and osmotic (500 mM sucrose) stress treatments. A. fragariae formed aggregates during desiccation and showed higher survival rates than A. avenae under desiccation and osmotic stress. Analysis of transcripts with Illumina RNA-seq indicated that glutaredoxin and other antioxidant-related genes were up-regulated under desiccation stress. Quantitative RT-PCR demonstrated 2.8 fold and 1.3 fold up-regulation of a glutaredoxin gene under desiccated and osmotic stress, respectively, suggesting the participation of antioxidant mechanisms in desiccation tolerance of A. fragariae.
Http Dx Doi Org 10 1080 01904160903470497, Jan 8, 2010
The relationship between root age and root physiology is poorly understood, despite its importanc... more The relationship between root age and root physiology is poorly understood, despite its importance for nutrient absorption. In peaches, roots are white when they first appear and then become brown with age, which corresponds to a number of physiological changes. We related root ...
PLOS ONE, 2015
Low-cost, high throughput genotyping methods are crucial to marker discovery and marker-assisted ... more Low-cost, high throughput genotyping methods are crucial to marker discovery and marker-assisted breeding efforts, but have not been available for many 'specialty crops' such as fruit and nut trees. Here we apply the Genotyping-By-Sequencing (GBS) method developed for cereals to the discovery of single nucleotide polymorphisms (SNPs) in a peach F2 mapping population. Peach is a genetic and genomic model within the Rosaceae and will provide a template for the use of this method with other members of this family. Our F2 mapping population of 57 genotypes segregates for bloom time (BD) and chilling requirement (CR) and we have extensively phenotyped this population. The population derives from a selfed F1 progeny of a cross between 'Hakuho' (high CR) and 'UFGold' (low CR). We were able to successfully employ GBS and the TASSEL GBS pipeline without modification of the original methodology using the ApeKI restriction enzyme and multiplexing at an equivalent of 96 samples per Illumina HiSeq 2000 lane. We obtained hundreds of SNP markers which were then used to construct a genetic linkage map and identify quantitative trait loci (QTL) for BD and CR.
VI International Peach Symposium, 2006
The foliar nematode Aphelenchoides fragariae (Aphelenchida: Aphelenchidae) is an endo- and ectopa... more The foliar nematode Aphelenchoides fragariae (Aphelenchida: Aphelenchidae) is an endo- and ectoparasite that feeds on aerial parts of over 250 plant species from 47 families. It is a common and economically-damaging pest of nursery-grown crops including ferns, foliage and flowering plants, and herbaceous and woody perennials. We used Illumina HiSeq 2000 technology and the Trinity de novo assembler (trinityrnaseq.sourceforge.net/) to produce a transcriptome of A. fragariae from four growth conditions (fungal-fed, plant-fed, diet-changed, and desiccated). High throughput sequencing generated 43 million reads with average length of 100-bp. Assembly of the pooled read set generated 95,930 contigs, corresponding to 50,686 unigenes (mean length = 605 bp) with 45,244 alternate splice variants. Fifty-two percent of the unigenes (26,389) had homology to known genes or proteins in the NCBI non-redundant database, and 20,558 were assigned GO terms based on these homologies by Blast2Go software...
Journal of Plant Nutrition, 2010
The relationship between root age and root physiology is poorly understood, despite its importanc... more The relationship between root age and root physiology is poorly understood, despite its importance for nutrient absorption. In peaches, roots are white when they first appear and then become brown with age, which corresponds to a number of physiological changes. We related root ...
Douglas G. Bielenberg: A.
... March 2009 Performance of Mycorrhizal Products Marketed for Woody Landscape Plants1 P. Eric W... more ... March 2009 Performance of Mycorrhizal Products Marketed for Woody Landscape Plants1 P. Eric Wiseman2, Kristen H. Colvin3, and Christina E. Wells4 Department of Horticulture, Clemson University, Clemson, SC 29634 Abstract ... 65.9 g/L 1, 2, 3, 5 Santa Maria, CA and one ...
Page 1. 70 J. Environ. Hort. 27(2):70–79. June 2009 Arbuscular Mycorrhizal Inoculation Affects Ro... more Page 1. 70 J. Environ. Hort. 27(2):70–79. June 2009 Arbuscular Mycorrhizal Inoculation Affects Root Development of Acer and Magnolia Species1 P. Eric Wiseman2 and Christina E. Wells3 Department of Forestry, Virginia Tech ...
Machine Vision and Applications
Journal of nematology
Desiccation tolerance plays an important role in the overwinter survival of the foliar nematode A... more Desiccation tolerance plays an important role in the overwinter survival of the foliar nematode Aphelenchoides fragariae. Survival rates of A. fragariae were compared with those of the anhydrobiotic soil-dwelling nematode Aphelenchus avenae after desiccation (90% RH), cold (4°C) and osmotic (500 mM sucrose) stress treatments. A. fragariae formed aggregates during desiccation and showed higher survival rates than A. avenae under desiccation and osmotic stress. Analysis of transcripts with Illumina RNA-seq indicated that glutaredoxin and other antioxidant-related genes were up-regulated under desiccation stress. Quantitative RT-PCR demonstrated 2.8 fold and 1.3 fold up-regulation of a glutaredoxin gene under desiccated and osmotic stress, respectively, suggesting the participation of antioxidant mechanisms in desiccation tolerance of A. fragariae.