Jouda Mediouni | University Of Carthage (original) (raw)
Papers by Jouda Mediouni
LWT, 2017
Encapsulation of Rosmarinus officinalis essential oils in chitosan-benzoic acid nanogel with enha... more Encapsulation of Rosmarinus officinalis essential oils in chitosan-benzoic acid nanogel with enhanced antibacterial activity in beef cutlet against Salmonella typhimurium during refrigerated storage
Tunisian Journal of Plant Protection, 2018
This work evaluated the chickpea leaf miner Liriomyza cicerina mass trapping technique as an alte... more This work evaluated the chickpea leaf miner Liriomyza cicerina mass trapping technique as an alternative to insecticide spraying. A trap density of 2000 per ha was used. Trials were conducted in Beja during 2015 and 2016 using Nour variety. Leaves were sampled weekly from all treated and control plots and observed under binocular microscope. Regarding the reduction in infestation at harvest, results showed reductions of 20.11 and 18.13% respectively for chemical and mass trapping treatments compared to control. Efficacy also was assessed on the basis of captures and infestations reductions compared to control, the yield and 100-seeds weight. Results showed significant difference (at P inferieur 0.05) between treatments, with 0.21 kg/m² grain yield for the control and 0.8 kg/m² for the chemical treatment and the mass trapping. Also, regarding the 100-seeds weight, it was 21.5g
Jouda Mediouni-Ben Jemâa, Nesrine Tersim, Emna Bous hih, Laboratoire de Biotechnologie Appliquée ... more Jouda Mediouni-Ben Jemâa, Nesrine Tersim, Emna Bous hih, Laboratoire de Biotechnologie Appliquée à l’Agriculture, INRAT, Un iversité de Carthage, Rue Hedi Karray, 2080, Ariana, Tunisia, Karima Taleb-Toudert, Faculté des Sciences Biologiques et des Sciences Agronomiques, Universit é Mouloud Mammeri, BP 17, Tizi-Ouzou, Algeria, and Mohamed Larbi Khouja, Laboratoire d’Ecologie et d’Amélioration Sylvo-Pastorale, INRGREF, Université de Carthage, Rue Hedi Karray, 2080, Ariana, Tunisia
International Journal of Agriculture and Biosciences, 2019
Applied Entomology and Zoology, Jul 11, 2017
Species from the genus Mayetiola are observed in the main cereal cultures of Tunisia. Some resear... more Species from the genus Mayetiola are observed in the main cereal cultures of Tunisia. Some researchers have studied M. destructor that attacks wheat and M. hordei that attacks barley. However, a third important species observed in oat, M. avenae, has not been studied and is not well documented in Tunisia. A method to easily separate the species is needed to clarify the occurrences of these gall midge species. This study aimed to first distinguish between the three species of gall midges by molecular characterization and second to reveal the phylogenetic relationships within and between the three species of Mayetiola collected from 5 different regions of northern Tunisia. To achieve these purposes, two regions of the mitochondrial DNA, cytochrome oxidase subunit I gene, and the 16S rRNA gene were amplified by polymerase chain reaction and sequenced. For each marker, a set of 75 individuals were used for DNA analysis. Phylogenetic trees were created using the DNA sequences of all samples from the 3 species. Results showed significant separation of the three different species into dissimilar clades. Each clade contained only specimens from the same species. Differences were observed between DNA sequences of the same species. The differences within the same species were not representative of geographical variations but coexisted within a population Therefore, using the COI and 16S rRNA genes as markers can clearly separate M. avenae, M. destructor and M. hordei.
International Journal of Tropical Insect Science, Oct 16, 2022
Acta horticulturae, Jul 1, 2013
Insect pests cause serious losses in quantity and quality of the stored products during storage. ... more Insect pests cause serious losses in quantity and quality of the stored products during storage. The lesser grain borer, Rhyzopertha dominica F. (Coleoptera: Bostrichidae) and the sawtoothed grain beetle Oryzaephilus surinamensis L. (Coleoptera: Silvanidae) are considered as major insect pests in storage in Tunisia and worldwide. The excessive use of synthetic insecticides associated with intensification in agriculture lead to health and environmental problems. This situation requires efforts to find safe, effective and viable alternatives. Natural products are well known to have a range of useful biological properties against insect pests. Recently, research on insecticidal and repellent properties of essential oils has received attention for stored-product protection. Essential oils and extracts from genus Eucalyptus are well known to posses these properties. This study was initiated to evaluate the repellency action of Eucalyptus astringens essential oil against adults of O. surinamensis and R. dominica. Essential oil was extracted by hydrodistillation using Clevenger modified apparatus. GC/MS analysis showed that the main components were α-pinene (29.83%), 1,8-cineole (17.29%) and transpinocarveol (3.39%). E. astringens essential oil exhibited a repellent action against the two insects. At the concentration, 0.08 µl/m 2 , the repellency percentage was respectively 55% for O. surinamensis and 58.75% for R. dominica after 24 h of exposure.
Acta horticulturae, Feb 1, 2010
During the past few decades, the need for safer means of pest management have become crucial due ... more During the past few decades, the need for safer means of pest management have become crucial due to the harmful effects of synthetic insecticides on the environment, problems related to pests' resurgence and resistance to pesticide and risk for users. Therefore, a growing interest in research concerning the possible use of plant extracts as alternatives to synthetic insecticides was developed. In this study, we report first investigations on chemical constituents and fumigant toxicity of Pistacia lentiscus L. (Anacardiacea) essential oil against 1-7 day-old adults of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) and Lasioderma serricorne (F.) (Coleoptera: Anobiidae). The major compounds of the essential oil determined using the GC and the GC-MS were: α-phellandrene (3.20%), α-pinene (9.48%) and limonene (19.11%). Oil amounts tested were 5 and 45 µl in each 44 ml Plexiglas bottle with capacity corresponding to concentrations 114 and 1023 µl/L air. Great differences in insect mortality were observed within insect species, oil concentrations and exposure time. The fumigant toxicity potential of P. lentiscus on L. serricorne was higher (LC 50 = 8.44 µl/L, LC 95 = 43.68 µl/L) than T. castaneum (LC 50 =28.03 µl/L, LC 95 =63.46 µl/L). The results suggested that P. lentiscus essential oil may have potential as a control agent against these two stored product beetles.
Journal of Agricultural Science and Technology, 2017
B-biotype Bemisia tabaci is a severe insect pest worldwide in many ornamental, agricultural, and ... more B-biotype Bemisia tabaci is a severe insect pest worldwide in many ornamental, agricultural, and horticultural crops. Control of this insect is obstructed by resistance to many AcetylCholinEsterase (AChE)-inhibiting insecticides, such as organophosphates and carbamates. In the present work, we evaluated the acetylcholinesterase inhibitory activity of six monoterpenoids namely α-pinene, terpineol, linalool, ß-myrcene, nerol and geraniol in vitro and in vivo. Inhibition of AChE of B. tabaci was measured by colorimetric method. The results showed that all of the monoterpenoids produced AChE inhibitory activity, with IC50 values ranging from 0.96 to 26.85 mM. Alpha-pinene showed the most potent inhibitory activity (IC50= 0.96 mM). Kinetic analysis showed reversible noncompetitive type inhibition, revealing that these components might bind both the enzyme alone and the enzyme-substrate. Results demonstrate the AChE inhibitory activity as mode of action of these monoterpenoids at relative...
Journal of Stored Products Research
Proceedings of The 1st International Electronic Conference on Entomology, Jun 30, 2021
The aim of this work is to evaluate the insecticidal activity of the encapsulatedrosemary (Rosmar... more The aim of this work is to evaluate the insecticidal activity of the encapsulatedrosemary (Rosmarinus officinalis L.) essential oil coated intochitosan matrix. The effectiveness of crude and encapsulated oils has been studied during different storage periods (30, 45 and 60 days). Results revealed that the chitosan-essential oil formulation exhibited high insecticidal activity against adults of Tribolium castaneum as compared to crude essential oil during the different storage periods.
Journal of natural pesticide research, Jun 1, 2022
Journal of Stored Products Research, Oct 1, 2010
... These polyphagous species attack several other host plants stored locally such as pomegranate... more ... These polyphagous species attack several other host plants stored locally such as pomegranate, almond and pistachio nut (Dhouibi, 1989) and cause major economic losses in the Mediterranean basin and Near East regions (Al-Izzi et al., 1985). ...
International Journal of Environmental Health Research, Aug 16, 2023
HAL (Le Centre pour la Communication Scientifique Directe), 2010
Journal of Plant Diseases and Protection, Mar 27, 2019
The aim of this research was to explore the chemical composition and evaluate the insecticidal ac... more The aim of this research was to explore the chemical composition and evaluate the insecticidal activities of Ruta graveolens, Mentha pulegium and Ocimum basilicum leaves essential oils from south of Tunisia. Fumigant toxicities were assessed toward the most important and destructive insects attacking dates in storage in Tunisia namely Ectomyelois ceratoniae and Ephestia kuehniella. GC–MS analyses showed that major compounds of O. basilicum essential oil were linalool (29.23%), methyl cinnamate (18.97%) and eugenol (5.84%), followed by 1,8-cineole (5.74%), α-cadinol (5.69%), estragol (4.72%) and α-bergamotene (4.24%), whereas M. pulegium essential oil had 1,8-cineole (14.60%), p-menthan-3-one (14.9%), piperitenone (11.4%), carene (10.19%), menthol (8.76%), trans-caryophyllene (6.64%) and menthyl alcohol (6.14%) as major components. The essential oil extracted from R. graveolens was remarkably rich in hydrocarbon ketones (52.5%) mainly 1-nonene (19.4%), 2-undecanone (16.22%) and 2-nonanone (11.9%). Results demonstrated that fumigant toxicity varied with insect species, essential oil concentration and exposure time. Lethal concentration (LC50) values of O. basilicum, M. pulegium and R. graveolens were, respectively, 0.96, 0.3 and 1.02 μL/L air on E. kuehniella and were 1.23, 0.31 and 1.97 μL/L air on E. ceratoniae. M. pulegium essential oil was more toxic to the two stored date pests E. kuehniella and E. ceratoniae at all tested concentrations.
LWT, 2017
Encapsulation of Rosmarinus officinalis essential oils in chitosan-benzoic acid nanogel with enha... more Encapsulation of Rosmarinus officinalis essential oils in chitosan-benzoic acid nanogel with enhanced antibacterial activity in beef cutlet against Salmonella typhimurium during refrigerated storage
Tunisian Journal of Plant Protection, 2018
This work evaluated the chickpea leaf miner Liriomyza cicerina mass trapping technique as an alte... more This work evaluated the chickpea leaf miner Liriomyza cicerina mass trapping technique as an alternative to insecticide spraying. A trap density of 2000 per ha was used. Trials were conducted in Beja during 2015 and 2016 using Nour variety. Leaves were sampled weekly from all treated and control plots and observed under binocular microscope. Regarding the reduction in infestation at harvest, results showed reductions of 20.11 and 18.13% respectively for chemical and mass trapping treatments compared to control. Efficacy also was assessed on the basis of captures and infestations reductions compared to control, the yield and 100-seeds weight. Results showed significant difference (at P inferieur 0.05) between treatments, with 0.21 kg/m² grain yield for the control and 0.8 kg/m² for the chemical treatment and the mass trapping. Also, regarding the 100-seeds weight, it was 21.5g
Jouda Mediouni-Ben Jemâa, Nesrine Tersim, Emna Bous hih, Laboratoire de Biotechnologie Appliquée ... more Jouda Mediouni-Ben Jemâa, Nesrine Tersim, Emna Bous hih, Laboratoire de Biotechnologie Appliquée à l’Agriculture, INRAT, Un iversité de Carthage, Rue Hedi Karray, 2080, Ariana, Tunisia, Karima Taleb-Toudert, Faculté des Sciences Biologiques et des Sciences Agronomiques, Universit é Mouloud Mammeri, BP 17, Tizi-Ouzou, Algeria, and Mohamed Larbi Khouja, Laboratoire d’Ecologie et d’Amélioration Sylvo-Pastorale, INRGREF, Université de Carthage, Rue Hedi Karray, 2080, Ariana, Tunisia
International Journal of Agriculture and Biosciences, 2019
Applied Entomology and Zoology, Jul 11, 2017
Species from the genus Mayetiola are observed in the main cereal cultures of Tunisia. Some resear... more Species from the genus Mayetiola are observed in the main cereal cultures of Tunisia. Some researchers have studied M. destructor that attacks wheat and M. hordei that attacks barley. However, a third important species observed in oat, M. avenae, has not been studied and is not well documented in Tunisia. A method to easily separate the species is needed to clarify the occurrences of these gall midge species. This study aimed to first distinguish between the three species of gall midges by molecular characterization and second to reveal the phylogenetic relationships within and between the three species of Mayetiola collected from 5 different regions of northern Tunisia. To achieve these purposes, two regions of the mitochondrial DNA, cytochrome oxidase subunit I gene, and the 16S rRNA gene were amplified by polymerase chain reaction and sequenced. For each marker, a set of 75 individuals were used for DNA analysis. Phylogenetic trees were created using the DNA sequences of all samples from the 3 species. Results showed significant separation of the three different species into dissimilar clades. Each clade contained only specimens from the same species. Differences were observed between DNA sequences of the same species. The differences within the same species were not representative of geographical variations but coexisted within a population Therefore, using the COI and 16S rRNA genes as markers can clearly separate M. avenae, M. destructor and M. hordei.
International Journal of Tropical Insect Science, Oct 16, 2022
Acta horticulturae, Jul 1, 2013
Insect pests cause serious losses in quantity and quality of the stored products during storage. ... more Insect pests cause serious losses in quantity and quality of the stored products during storage. The lesser grain borer, Rhyzopertha dominica F. (Coleoptera: Bostrichidae) and the sawtoothed grain beetle Oryzaephilus surinamensis L. (Coleoptera: Silvanidae) are considered as major insect pests in storage in Tunisia and worldwide. The excessive use of synthetic insecticides associated with intensification in agriculture lead to health and environmental problems. This situation requires efforts to find safe, effective and viable alternatives. Natural products are well known to have a range of useful biological properties against insect pests. Recently, research on insecticidal and repellent properties of essential oils has received attention for stored-product protection. Essential oils and extracts from genus Eucalyptus are well known to posses these properties. This study was initiated to evaluate the repellency action of Eucalyptus astringens essential oil against adults of O. surinamensis and R. dominica. Essential oil was extracted by hydrodistillation using Clevenger modified apparatus. GC/MS analysis showed that the main components were α-pinene (29.83%), 1,8-cineole (17.29%) and transpinocarveol (3.39%). E. astringens essential oil exhibited a repellent action against the two insects. At the concentration, 0.08 µl/m 2 , the repellency percentage was respectively 55% for O. surinamensis and 58.75% for R. dominica after 24 h of exposure.
Acta horticulturae, Feb 1, 2010
During the past few decades, the need for safer means of pest management have become crucial due ... more During the past few decades, the need for safer means of pest management have become crucial due to the harmful effects of synthetic insecticides on the environment, problems related to pests' resurgence and resistance to pesticide and risk for users. Therefore, a growing interest in research concerning the possible use of plant extracts as alternatives to synthetic insecticides was developed. In this study, we report first investigations on chemical constituents and fumigant toxicity of Pistacia lentiscus L. (Anacardiacea) essential oil against 1-7 day-old adults of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) and Lasioderma serricorne (F.) (Coleoptera: Anobiidae). The major compounds of the essential oil determined using the GC and the GC-MS were: α-phellandrene (3.20%), α-pinene (9.48%) and limonene (19.11%). Oil amounts tested were 5 and 45 µl in each 44 ml Plexiglas bottle with capacity corresponding to concentrations 114 and 1023 µl/L air. Great differences in insect mortality were observed within insect species, oil concentrations and exposure time. The fumigant toxicity potential of P. lentiscus on L. serricorne was higher (LC 50 = 8.44 µl/L, LC 95 = 43.68 µl/L) than T. castaneum (LC 50 =28.03 µl/L, LC 95 =63.46 µl/L). The results suggested that P. lentiscus essential oil may have potential as a control agent against these two stored product beetles.
Journal of Agricultural Science and Technology, 2017
B-biotype Bemisia tabaci is a severe insect pest worldwide in many ornamental, agricultural, and ... more B-biotype Bemisia tabaci is a severe insect pest worldwide in many ornamental, agricultural, and horticultural crops. Control of this insect is obstructed by resistance to many AcetylCholinEsterase (AChE)-inhibiting insecticides, such as organophosphates and carbamates. In the present work, we evaluated the acetylcholinesterase inhibitory activity of six monoterpenoids namely α-pinene, terpineol, linalool, ß-myrcene, nerol and geraniol in vitro and in vivo. Inhibition of AChE of B. tabaci was measured by colorimetric method. The results showed that all of the monoterpenoids produced AChE inhibitory activity, with IC50 values ranging from 0.96 to 26.85 mM. Alpha-pinene showed the most potent inhibitory activity (IC50= 0.96 mM). Kinetic analysis showed reversible noncompetitive type inhibition, revealing that these components might bind both the enzyme alone and the enzyme-substrate. Results demonstrate the AChE inhibitory activity as mode of action of these monoterpenoids at relative...
Journal of Stored Products Research
Proceedings of The 1st International Electronic Conference on Entomology, Jun 30, 2021
The aim of this work is to evaluate the insecticidal activity of the encapsulatedrosemary (Rosmar... more The aim of this work is to evaluate the insecticidal activity of the encapsulatedrosemary (Rosmarinus officinalis L.) essential oil coated intochitosan matrix. The effectiveness of crude and encapsulated oils has been studied during different storage periods (30, 45 and 60 days). Results revealed that the chitosan-essential oil formulation exhibited high insecticidal activity against adults of Tribolium castaneum as compared to crude essential oil during the different storage periods.
Journal of natural pesticide research, Jun 1, 2022
Journal of Stored Products Research, Oct 1, 2010
... These polyphagous species attack several other host plants stored locally such as pomegranate... more ... These polyphagous species attack several other host plants stored locally such as pomegranate, almond and pistachio nut (Dhouibi, 1989) and cause major economic losses in the Mediterranean basin and Near East regions (Al-Izzi et al., 1985). ...
International Journal of Environmental Health Research, Aug 16, 2023
HAL (Le Centre pour la Communication Scientifique Directe), 2010
Journal of Plant Diseases and Protection, Mar 27, 2019
The aim of this research was to explore the chemical composition and evaluate the insecticidal ac... more The aim of this research was to explore the chemical composition and evaluate the insecticidal activities of Ruta graveolens, Mentha pulegium and Ocimum basilicum leaves essential oils from south of Tunisia. Fumigant toxicities were assessed toward the most important and destructive insects attacking dates in storage in Tunisia namely Ectomyelois ceratoniae and Ephestia kuehniella. GC–MS analyses showed that major compounds of O. basilicum essential oil were linalool (29.23%), methyl cinnamate (18.97%) and eugenol (5.84%), followed by 1,8-cineole (5.74%), α-cadinol (5.69%), estragol (4.72%) and α-bergamotene (4.24%), whereas M. pulegium essential oil had 1,8-cineole (14.60%), p-menthan-3-one (14.9%), piperitenone (11.4%), carene (10.19%), menthol (8.76%), trans-caryophyllene (6.64%) and menthyl alcohol (6.14%) as major components. The essential oil extracted from R. graveolens was remarkably rich in hydrocarbon ketones (52.5%) mainly 1-nonene (19.4%), 2-undecanone (16.22%) and 2-nonanone (11.9%). Results demonstrated that fumigant toxicity varied with insect species, essential oil concentration and exposure time. Lethal concentration (LC50) values of O. basilicum, M. pulegium and R. graveolens were, respectively, 0.96, 0.3 and 1.02 μL/L air on E. kuehniella and were 1.23, 0.31 and 1.97 μL/L air on E. ceratoniae. M. pulegium essential oil was more toxic to the two stored date pests E. kuehniella and E. ceratoniae at all tested concentrations.