Jouda Mediouni | University Of Carthage (original) (raw)

Papers by Jouda Mediouni

Research paper thumbnail of Encapsulation of Rosmarinus officinalis essential oils in chitosan-benzoic acid nanogel with enhanced antibacterial activity in beef cutlet against Salmonella typhimurium during refrigerated storage

LWT, 2017

Encapsulation of Rosmarinus officinalis essential oils in chitosan-benzoic acid nanogel with enha... more Encapsulation of Rosmarinus officinalis essential oils in chitosan-benzoic acid nanogel with enhanced antibacterial activity in beef cutlet against Salmonella typhimurium during refrigerated storage

Research paper thumbnail of Field Assessment of the Mass Trapping Technique for the Control of the Chickpea Leaf Miner Liriomyza cicerina

Tunisian Journal of Plant Protection, 2018

This work evaluated the chickpea leaf miner Liriomyza cicerina mass trapping technique as an alte... more This work evaluated the chickpea leaf miner Liriomyza cicerina mass trapping technique as an alternative to insecticide spraying. A trap density of 2000 per ha was used. Trials were conducted in Beja during 2015 and 2016 using Nour variety. Leaves were sampled weekly from all treated and control plots and observed under binocular microscope. Regarding the reduction in infestation at harvest, results showed reductions of 20.11 and 18.13% respectively for chemical and mass trapping treatments compared to control. Efficacy also was assessed on the basis of captures and infestations reductions compared to control, the yield and 100-seeds weight. Results showed significant difference (at P inferieur 0.05) between treatments, with 0.21 kg/m² grain yield for the control and 0.8 kg/m² for the chemical treatment and the mass trapping. Also, regarding the 100-seeds weight, it was 21.5g

Research paper thumbnail of Fumigant Control of the Mediterranean Flour Moth Ephestia kuehniella with the Noble Laurel Laurus nobilis Essential Oils

Jouda Mediouni-Ben Jemâa, Nesrine Tersim, Emna Bous hih, Laboratoire de Biotechnologie Appliquée ... more Jouda Mediouni-Ben Jemâa, Nesrine Tersim, Emna Bous hih, Laboratoire de Biotechnologie Appliquée à l’Agriculture, INRAT, Un iversité de Carthage, Rue Hedi Karray, 2080, Ariana, Tunisia, Karima Taleb-Toudert, Faculté des Sciences Biologiques et des Sciences Agronomiques, Universit é Mouloud Mammeri, BP 17, Tizi-Ouzou, Algeria, and Mohamed Larbi Khouja, Laboratoire d’Ecologie et d’Amélioration Sylvo-Pastorale, INRGREF, Université de Carthage, Rue Hedi Karray, 2080, Ariana, Tunisia

Research paper thumbnail of Saponin content of Agave americana (L.) leaf extract and its antifungal attributes against phytopathogenic fungi

International Journal of Agriculture and Biosciences, 2019

Research paper thumbnail of Characterization of Artemisia absinthium essential oil and evaluation of its insecticidal activities against two stored product beetles

Research paper thumbnail of Molecular characterization and phylogenetic comparisons of three Mayetiola species (Diptera: Cecidomyiidae) infesting cereals in Tunisia

Applied Entomology and Zoology, Jul 11, 2017

Species from the genus Mayetiola are observed in the main cereal cultures of Tunisia. Some resear... more Species from the genus Mayetiola are observed in the main cereal cultures of Tunisia. Some researchers have studied M. destructor that attacks wheat and M. hordei that attacks barley. However, a third important species observed in oat, M. avenae, has not been studied and is not well documented in Tunisia. A method to easily separate the species is needed to clarify the occurrences of these gall midge species. This study aimed to first distinguish between the three species of gall midges by molecular characterization and second to reveal the phylogenetic relationships within and between the three species of Mayetiola collected from 5 different regions of northern Tunisia. To achieve these purposes, two regions of the mitochondrial DNA, cytochrome oxidase subunit I gene, and the 16S rRNA gene were amplified by polymerase chain reaction and sequenced. For each marker, a set of 75 individuals were used for DNA analysis. Phylogenetic trees were created using the DNA sequences of all samples from the 3 species. Results showed significant separation of the three different species into dissimilar clades. Each clade contained only specimens from the same species. Differences were observed between DNA sequences of the same species. The differences within the same species were not representative of geographical variations but coexisted within a population Therefore, using the COI and 16S rRNA genes as markers can clearly separate M. avenae, M. destructor and M. hordei.

Research paper thumbnail of Effects of free and encapsulated Rosmarinus officinalis (L.) essential oil against Carpophilus hemipterus (Nitidulidae) and its impact on dates’ quality during different storage periods

International Journal of Tropical Insect Science, Oct 16, 2022

Research paper thumbnail of Repellent Activity of Essential Oil of Eucalyptus Astringens Against Rhyzopertha Dominica and Oryzaephilus Surinamensis

Acta horticulturae, Jul 1, 2013

Insect pests cause serious losses in quantity and quality of the stored products during storage. ... more Insect pests cause serious losses in quantity and quality of the stored products during storage. The lesser grain borer, Rhyzopertha dominica F. (Coleoptera: Bostrichidae) and the sawtoothed grain beetle Oryzaephilus surinamensis L. (Coleoptera: Silvanidae) are considered as major insect pests in storage in Tunisia and worldwide. The excessive use of synthetic insecticides associated with intensification in agriculture lead to health and environmental problems. This situation requires efforts to find safe, effective and viable alternatives. Natural products are well known to have a range of useful biological properties against insect pests. Recently, research on insecticidal and repellent properties of essential oils has received attention for stored-product protection. Essential oils and extracts from genus Eucalyptus are well known to posses these properties. This study was initiated to evaluate the repellency action of Eucalyptus astringens essential oil against adults of O. surinamensis and R. dominica. Essential oil was extracted by hydrodistillation using Clevenger modified apparatus. GC/MS analysis showed that the main components were α-pinene (29.83%), 1,8-cineole (17.29%) and transpinocarveol (3.39%). E. astringens essential oil exhibited a repellent action against the two insects. At the concentration, 0.08 µl/m 2 , the repellency percentage was respectively 55% for O. surinamensis and 58.75% for R. dominica after 24 h of exposure.

Research paper thumbnail of Fumigant Toxicity of Essential Oil from Pistacia Lentiscus L. (Anacardiacea) Against Stored-Product Insects

Acta horticulturae, Feb 1, 2010

During the past few decades, the need for safer means of pest management have become crucial due ... more During the past few decades, the need for safer means of pest management have become crucial due to the harmful effects of synthetic insecticides on the environment, problems related to pests' resurgence and resistance to pesticide and risk for users. Therefore, a growing interest in research concerning the possible use of plant extracts as alternatives to synthetic insecticides was developed. In this study, we report first investigations on chemical constituents and fumigant toxicity of Pistacia lentiscus L. (Anacardiacea) essential oil against 1-7 day-old adults of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) and Lasioderma serricorne (F.) (Coleoptera: Anobiidae). The major compounds of the essential oil determined using the GC and the GC-MS were: α-phellandrene (3.20%), α-pinene (9.48%) and limonene (19.11%). Oil amounts tested were 5 and 45 µl in each 44 ml Plexiglas bottle with capacity corresponding to concentrations 114 and 1023 µl/L air. Great differences in insect mortality were observed within insect species, oil concentrations and exposure time. The fumigant toxicity potential of P. lentiscus on L. serricorne was higher (LC 50 = 8.44 µl/L, LC 95 = 43.68 µl/L) than T. castaneum (LC 50 =28.03 µl/L, LC 95 =63.46 µl/L). The results suggested that P. lentiscus essential oil may have potential as a control agent against these two stored product beetles.

Research paper thumbnail of Anticholinesterase Potential of Monoterpenoids on the Whitefly Bemisia tabaci and Their Kinetic Studies

Journal of Agricultural Science and Technology, 2017

B-biotype Bemisia tabaci is a severe insect pest worldwide in many ornamental, agricultural, and ... more B-biotype Bemisia tabaci is a severe insect pest worldwide in many ornamental, agricultural, and horticultural crops. Control of this insect is obstructed by resistance to many AcetylCholinEsterase (AChE)-inhibiting insecticides, such as organophosphates and carbamates. In the present work, we evaluated the acetylcholinesterase inhibitory activity of six monoterpenoids namely α-pinene, terpineol, linalool, ß-myrcene, nerol and geraniol in vitro and in vivo. Inhibition of AChE of B. tabaci was measured by colorimetric method. The results showed that all of the monoterpenoids produced AChE inhibitory activity, with IC50 values ranging from 0.96 to 26.85 mM. Alpha-pinene showed the most potent inhibitory activity (IC50= 0.96 mM). Kinetic analysis showed reversible noncompetitive type inhibition, revealing that these components might bind both the enzyme alone and the enzyme-substrate. Results demonstrate the AChE inhibitory activity as mode of action of these monoterpenoids at relative...

Research paper thumbnail of Formulation of pectin-Citrus aurantium essential oil-based packaging film and assessment of insecticidal performances against Rhyzopertha dominica on stored wheat

Journal of Stored Products Research

Research paper thumbnail of Development of formulation based on essential oils of rosemary to manage pests of stored cereal foodstuffs

Proceedings of The 1st International Electronic Conference on Entomology, Jun 30, 2021

The aim of this work is to evaluate the insecticidal activity of the encapsulatedrosemary (Rosmar... more The aim of this work is to evaluate the insecticidal activity of the encapsulatedrosemary (Rosmarinus officinalis L.) essential oil coated intochitosan matrix. The effectiveness of crude and encapsulated oils has been studied during different storage periods (30, 45 and 60 days). Results revealed that the chitosan-essential oil formulation exhibited high insecticidal activity against adults of Tribolium castaneum as compared to crude essential oil during the different storage periods.

Research paper thumbnail of Insecticidal effects of natural products in free and encapsulated forms: an overview

Journal of natural pesticide research, Jun 1, 2022

Research paper thumbnail of Composition and insecticidal activity of essential oil from Pistacia lentiscus L. against Ectomyelois ceratoniae Zeller and Ephestia kuehniella Zeller (Lepidoptera: Pyralidae)

Journal of Stored Products Research, Oct 1, 2010

... These polyphagous species attack several other host plants stored locally such as pomegranate... more ... These polyphagous species attack several other host plants stored locally such as pomegranate, almond and pistachio nut (Dhouibi, 1989) and cause major economic losses in the Mediterranean basin and Near East regions (Al-Izzi et al., 1985). ...

Research paper thumbnail of Postharvest control alternatives for the management of the moth Ectomyelois ceratoniae Zeller (Pyralidae) in Tunisia: essential oil fumigation and its impacts on date quality

Research paper thumbnail of Eco-friendly management strategies of insect pests: long-term performance of rosemary essential oil encapsulated into chitosan and gum Arabic

International Journal of Environmental Health Research, Aug 16, 2023

Research paper thumbnail of Valorisation of the repellent potential of two Tunisian Artemisia essential oils for the management of the sawtoothed grain beetle Oryzaephilus surinamensis (Coleoptera: Silvanidae)

Research paper thumbnail of IPM approaches for stored date protection in Tunisia: emphasis on alternative control methods against the date moth Ectomyelois ceratoniae Zeller (Lepidoptera: Pyralidae)

Research paper thumbnail of Fumigant toxicity of <em>Pistacia lentiscus</em> essential oil against <em>Tribolium castaneum</em> and <em>Lasioderma serricorne</em>

HAL (Le Centre pour la Communication Scientifique Directe), 2010

Research paper thumbnail of Composition and insecticidal activity of essential oil from Ruta graveolens, Mentha pulegium and Ocimum basilicum against Ectomyelois ceratoniae Zeller and Ephestia kuehniella Zeller (Lepidoptera: Pyralidae)

Journal of Plant Diseases and Protection, Mar 27, 2019

The aim of this research was to explore the chemical composition and evaluate the insecticidal ac... more The aim of this research was to explore the chemical composition and evaluate the insecticidal activities of Ruta graveolens, Mentha pulegium and Ocimum basilicum leaves essential oils from south of Tunisia. Fumigant toxicities were assessed toward the most important and destructive insects attacking dates in storage in Tunisia namely Ectomyelois ceratoniae and Ephestia kuehniella. GC–MS analyses showed that major compounds of O. basilicum essential oil were linalool (29.23%), methyl cinnamate (18.97%) and eugenol (5.84%), followed by 1,8-cineole (5.74%), α-cadinol (5.69%), estragol (4.72%) and α-bergamotene (4.24%), whereas M. pulegium essential oil had 1,8-cineole (14.60%), p-menthan-3-one (14.9%), piperitenone (11.4%), carene (10.19%), menthol (8.76%), trans-caryophyllene (6.64%) and menthyl alcohol (6.14%) as major components. The essential oil extracted from R. graveolens was remarkably rich in hydrocarbon ketones (52.5%) mainly 1-nonene (19.4%), 2-undecanone (16.22%) and 2-nonanone (11.9%). Results demonstrated that fumigant toxicity varied with insect species, essential oil concentration and exposure time. Lethal concentration (LC50) values of O. basilicum, M. pulegium and R. graveolens were, respectively, 0.96, 0.3 and 1.02 μL/L air on E. kuehniella and were 1.23, 0.31 and 1.97 μL/L air on E. ceratoniae. M. pulegium essential oil was more toxic to the two stored date pests E. kuehniella and E. ceratoniae at all tested concentrations.

Research paper thumbnail of Encapsulation of Rosmarinus officinalis essential oils in chitosan-benzoic acid nanogel with enhanced antibacterial activity in beef cutlet against Salmonella typhimurium during refrigerated storage

LWT, 2017

Encapsulation of Rosmarinus officinalis essential oils in chitosan-benzoic acid nanogel with enha... more Encapsulation of Rosmarinus officinalis essential oils in chitosan-benzoic acid nanogel with enhanced antibacterial activity in beef cutlet against Salmonella typhimurium during refrigerated storage

Research paper thumbnail of Field Assessment of the Mass Trapping Technique for the Control of the Chickpea Leaf Miner Liriomyza cicerina

Tunisian Journal of Plant Protection, 2018

This work evaluated the chickpea leaf miner Liriomyza cicerina mass trapping technique as an alte... more This work evaluated the chickpea leaf miner Liriomyza cicerina mass trapping technique as an alternative to insecticide spraying. A trap density of 2000 per ha was used. Trials were conducted in Beja during 2015 and 2016 using Nour variety. Leaves were sampled weekly from all treated and control plots and observed under binocular microscope. Regarding the reduction in infestation at harvest, results showed reductions of 20.11 and 18.13% respectively for chemical and mass trapping treatments compared to control. Efficacy also was assessed on the basis of captures and infestations reductions compared to control, the yield and 100-seeds weight. Results showed significant difference (at P inferieur 0.05) between treatments, with 0.21 kg/m² grain yield for the control and 0.8 kg/m² for the chemical treatment and the mass trapping. Also, regarding the 100-seeds weight, it was 21.5g

Research paper thumbnail of Fumigant Control of the Mediterranean Flour Moth Ephestia kuehniella with the Noble Laurel Laurus nobilis Essential Oils

Jouda Mediouni-Ben Jemâa, Nesrine Tersim, Emna Bous hih, Laboratoire de Biotechnologie Appliquée ... more Jouda Mediouni-Ben Jemâa, Nesrine Tersim, Emna Bous hih, Laboratoire de Biotechnologie Appliquée à l’Agriculture, INRAT, Un iversité de Carthage, Rue Hedi Karray, 2080, Ariana, Tunisia, Karima Taleb-Toudert, Faculté des Sciences Biologiques et des Sciences Agronomiques, Universit é Mouloud Mammeri, BP 17, Tizi-Ouzou, Algeria, and Mohamed Larbi Khouja, Laboratoire d’Ecologie et d’Amélioration Sylvo-Pastorale, INRGREF, Université de Carthage, Rue Hedi Karray, 2080, Ariana, Tunisia

Research paper thumbnail of Saponin content of Agave americana (L.) leaf extract and its antifungal attributes against phytopathogenic fungi

International Journal of Agriculture and Biosciences, 2019

Research paper thumbnail of Characterization of Artemisia absinthium essential oil and evaluation of its insecticidal activities against two stored product beetles

Research paper thumbnail of Molecular characterization and phylogenetic comparisons of three Mayetiola species (Diptera: Cecidomyiidae) infesting cereals in Tunisia

Applied Entomology and Zoology, Jul 11, 2017

Species from the genus Mayetiola are observed in the main cereal cultures of Tunisia. Some resear... more Species from the genus Mayetiola are observed in the main cereal cultures of Tunisia. Some researchers have studied M. destructor that attacks wheat and M. hordei that attacks barley. However, a third important species observed in oat, M. avenae, has not been studied and is not well documented in Tunisia. A method to easily separate the species is needed to clarify the occurrences of these gall midge species. This study aimed to first distinguish between the three species of gall midges by molecular characterization and second to reveal the phylogenetic relationships within and between the three species of Mayetiola collected from 5 different regions of northern Tunisia. To achieve these purposes, two regions of the mitochondrial DNA, cytochrome oxidase subunit I gene, and the 16S rRNA gene were amplified by polymerase chain reaction and sequenced. For each marker, a set of 75 individuals were used for DNA analysis. Phylogenetic trees were created using the DNA sequences of all samples from the 3 species. Results showed significant separation of the three different species into dissimilar clades. Each clade contained only specimens from the same species. Differences were observed between DNA sequences of the same species. The differences within the same species were not representative of geographical variations but coexisted within a population Therefore, using the COI and 16S rRNA genes as markers can clearly separate M. avenae, M. destructor and M. hordei.

Research paper thumbnail of Effects of free and encapsulated Rosmarinus officinalis (L.) essential oil against Carpophilus hemipterus (Nitidulidae) and its impact on dates’ quality during different storage periods

International Journal of Tropical Insect Science, Oct 16, 2022

Research paper thumbnail of Repellent Activity of Essential Oil of Eucalyptus Astringens Against Rhyzopertha Dominica and Oryzaephilus Surinamensis

Acta horticulturae, Jul 1, 2013

Insect pests cause serious losses in quantity and quality of the stored products during storage. ... more Insect pests cause serious losses in quantity and quality of the stored products during storage. The lesser grain borer, Rhyzopertha dominica F. (Coleoptera: Bostrichidae) and the sawtoothed grain beetle Oryzaephilus surinamensis L. (Coleoptera: Silvanidae) are considered as major insect pests in storage in Tunisia and worldwide. The excessive use of synthetic insecticides associated with intensification in agriculture lead to health and environmental problems. This situation requires efforts to find safe, effective and viable alternatives. Natural products are well known to have a range of useful biological properties against insect pests. Recently, research on insecticidal and repellent properties of essential oils has received attention for stored-product protection. Essential oils and extracts from genus Eucalyptus are well known to posses these properties. This study was initiated to evaluate the repellency action of Eucalyptus astringens essential oil against adults of O. surinamensis and R. dominica. Essential oil was extracted by hydrodistillation using Clevenger modified apparatus. GC/MS analysis showed that the main components were α-pinene (29.83%), 1,8-cineole (17.29%) and transpinocarveol (3.39%). E. astringens essential oil exhibited a repellent action against the two insects. At the concentration, 0.08 µl/m 2 , the repellency percentage was respectively 55% for O. surinamensis and 58.75% for R. dominica after 24 h of exposure.

Research paper thumbnail of Fumigant Toxicity of Essential Oil from Pistacia Lentiscus L. (Anacardiacea) Against Stored-Product Insects

Acta horticulturae, Feb 1, 2010

During the past few decades, the need for safer means of pest management have become crucial due ... more During the past few decades, the need for safer means of pest management have become crucial due to the harmful effects of synthetic insecticides on the environment, problems related to pests' resurgence and resistance to pesticide and risk for users. Therefore, a growing interest in research concerning the possible use of plant extracts as alternatives to synthetic insecticides was developed. In this study, we report first investigations on chemical constituents and fumigant toxicity of Pistacia lentiscus L. (Anacardiacea) essential oil against 1-7 day-old adults of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) and Lasioderma serricorne (F.) (Coleoptera: Anobiidae). The major compounds of the essential oil determined using the GC and the GC-MS were: α-phellandrene (3.20%), α-pinene (9.48%) and limonene (19.11%). Oil amounts tested were 5 and 45 µl in each 44 ml Plexiglas bottle with capacity corresponding to concentrations 114 and 1023 µl/L air. Great differences in insect mortality were observed within insect species, oil concentrations and exposure time. The fumigant toxicity potential of P. lentiscus on L. serricorne was higher (LC 50 = 8.44 µl/L, LC 95 = 43.68 µl/L) than T. castaneum (LC 50 =28.03 µl/L, LC 95 =63.46 µl/L). The results suggested that P. lentiscus essential oil may have potential as a control agent against these two stored product beetles.

Research paper thumbnail of Anticholinesterase Potential of Monoterpenoids on the Whitefly Bemisia tabaci and Their Kinetic Studies

Journal of Agricultural Science and Technology, 2017

B-biotype Bemisia tabaci is a severe insect pest worldwide in many ornamental, agricultural, and ... more B-biotype Bemisia tabaci is a severe insect pest worldwide in many ornamental, agricultural, and horticultural crops. Control of this insect is obstructed by resistance to many AcetylCholinEsterase (AChE)-inhibiting insecticides, such as organophosphates and carbamates. In the present work, we evaluated the acetylcholinesterase inhibitory activity of six monoterpenoids namely α-pinene, terpineol, linalool, ß-myrcene, nerol and geraniol in vitro and in vivo. Inhibition of AChE of B. tabaci was measured by colorimetric method. The results showed that all of the monoterpenoids produced AChE inhibitory activity, with IC50 values ranging from 0.96 to 26.85 mM. Alpha-pinene showed the most potent inhibitory activity (IC50= 0.96 mM). Kinetic analysis showed reversible noncompetitive type inhibition, revealing that these components might bind both the enzyme alone and the enzyme-substrate. Results demonstrate the AChE inhibitory activity as mode of action of these monoterpenoids at relative...

Research paper thumbnail of Formulation of pectin-Citrus aurantium essential oil-based packaging film and assessment of insecticidal performances against Rhyzopertha dominica on stored wheat

Journal of Stored Products Research

Research paper thumbnail of Development of formulation based on essential oils of rosemary to manage pests of stored cereal foodstuffs

Proceedings of The 1st International Electronic Conference on Entomology, Jun 30, 2021

The aim of this work is to evaluate the insecticidal activity of the encapsulatedrosemary (Rosmar... more The aim of this work is to evaluate the insecticidal activity of the encapsulatedrosemary (Rosmarinus officinalis L.) essential oil coated intochitosan matrix. The effectiveness of crude and encapsulated oils has been studied during different storage periods (30, 45 and 60 days). Results revealed that the chitosan-essential oil formulation exhibited high insecticidal activity against adults of Tribolium castaneum as compared to crude essential oil during the different storage periods.

Research paper thumbnail of Insecticidal effects of natural products in free and encapsulated forms: an overview

Journal of natural pesticide research, Jun 1, 2022

Research paper thumbnail of Composition and insecticidal activity of essential oil from Pistacia lentiscus L. against Ectomyelois ceratoniae Zeller and Ephestia kuehniella Zeller (Lepidoptera: Pyralidae)

Journal of Stored Products Research, Oct 1, 2010

... These polyphagous species attack several other host plants stored locally such as pomegranate... more ... These polyphagous species attack several other host plants stored locally such as pomegranate, almond and pistachio nut (Dhouibi, 1989) and cause major economic losses in the Mediterranean basin and Near East regions (Al-Izzi et al., 1985). ...

Research paper thumbnail of Postharvest control alternatives for the management of the moth Ectomyelois ceratoniae Zeller (Pyralidae) in Tunisia: essential oil fumigation and its impacts on date quality

Research paper thumbnail of Eco-friendly management strategies of insect pests: long-term performance of rosemary essential oil encapsulated into chitosan and gum Arabic

International Journal of Environmental Health Research, Aug 16, 2023

Research paper thumbnail of Valorisation of the repellent potential of two Tunisian Artemisia essential oils for the management of the sawtoothed grain beetle Oryzaephilus surinamensis (Coleoptera: Silvanidae)

Research paper thumbnail of IPM approaches for stored date protection in Tunisia: emphasis on alternative control methods against the date moth Ectomyelois ceratoniae Zeller (Lepidoptera: Pyralidae)

Research paper thumbnail of Fumigant toxicity of <em>Pistacia lentiscus</em> essential oil against <em>Tribolium castaneum</em> and <em>Lasioderma serricorne</em>

HAL (Le Centre pour la Communication Scientifique Directe), 2010

Research paper thumbnail of Composition and insecticidal activity of essential oil from Ruta graveolens, Mentha pulegium and Ocimum basilicum against Ectomyelois ceratoniae Zeller and Ephestia kuehniella Zeller (Lepidoptera: Pyralidae)

Journal of Plant Diseases and Protection, Mar 27, 2019

The aim of this research was to explore the chemical composition and evaluate the insecticidal ac... more The aim of this research was to explore the chemical composition and evaluate the insecticidal activities of Ruta graveolens, Mentha pulegium and Ocimum basilicum leaves essential oils from south of Tunisia. Fumigant toxicities were assessed toward the most important and destructive insects attacking dates in storage in Tunisia namely Ectomyelois ceratoniae and Ephestia kuehniella. GC–MS analyses showed that major compounds of O. basilicum essential oil were linalool (29.23%), methyl cinnamate (18.97%) and eugenol (5.84%), followed by 1,8-cineole (5.74%), α-cadinol (5.69%), estragol (4.72%) and α-bergamotene (4.24%), whereas M. pulegium essential oil had 1,8-cineole (14.60%), p-menthan-3-one (14.9%), piperitenone (11.4%), carene (10.19%), menthol (8.76%), trans-caryophyllene (6.64%) and menthyl alcohol (6.14%) as major components. The essential oil extracted from R. graveolens was remarkably rich in hydrocarbon ketones (52.5%) mainly 1-nonene (19.4%), 2-undecanone (16.22%) and 2-nonanone (11.9%). Results demonstrated that fumigant toxicity varied with insect species, essential oil concentration and exposure time. Lethal concentration (LC50) values of O. basilicum, M. pulegium and R. graveolens were, respectively, 0.96, 0.3 and 1.02 μL/L air on E. kuehniella and were 1.23, 0.31 and 1.97 μL/L air on E. ceratoniae. M. pulegium essential oil was more toxic to the two stored date pests E. kuehniella and E. ceratoniae at all tested concentrations.