Bruna Matturro | Consiglio Nazionale delle Ricerche (CNR) (original) (raw)

Papers by Bruna Matturro

Research paper thumbnail of Cable Bacteria and the Bioelectrochemical Snorkel: The Natural and Engineered Facets Playing a Role in Hydrocarbons Degradation in Marine Sediments (Bruna Matturro, Carolina Cruz Viggi, Federico Aulenta and Simona Rossetti) —> Please cite this article as Matturro et al., 2017

The composition and metabolic traits of the microbial communities acting in an innovative bioelec... more The composition and metabolic traits of the microbial communities acting in an innovative bioelectrochemical system were here investigated. The system, known as Oil Spill Snorkel, was recently developed to stimulate the oxidative biodegradation of petroleum hydrocarbons in anoxic marine sediments. Next Generation Sequencing was used to describe the microbiome of the bulk sediment and of the biofilm growing attached to the surface of the electrode. The analysis revealed that sulfur cycling primarily drives the microbial metabolic activities occurring in the bioelectrochemical system. In the anoxic zone of the contaminated marine sediment, petroleum hydrocarbon degradation occurred under sulfate-reducing conditions and was lead by different families of Desulfobacterales (46% of total OTUs). Remarkably, the occurrence of filamentous Desulfubulbaceae, known to be capable to vehicle electrons deriving from sulfide oxidation to oxygen serving as a spatially distant electron acceptor, was demonstrated. Differently from the sediment, which was mostly colonized by Deltaproteobacteria, the biofilm at the anode hosted, at high extent, members of Alphaproteobacteria (59%) mostly affiliated to Rhodospirillaceae family (33%) and including several known sulfur-and sulfide-oxidizing genera. Overall, we showed the occurrence in the system of a variety of electroactive microorganisms able to sustain the contaminant biodegradation alone or by means of an external conductive support through the establishment of a bioelectrochemical connection between two spatially separated redox zones and the preservation of an efficient sulfur cycling.

Research paper thumbnail of Microbiome Dynamics of a Polychlorobiphenyl (PCB) Historically Contaminated Marine Sediment under Conditions Promoting Reductive Dechlorination (B. Matturro, C. Ubaldi, S. Rossetti) —> Please cite this article as Matturro et al., 2016

The toxicity of polychlorinated biphenyls (PCB) can be efficiently reduced in contaminated marine... more The toxicity of polychlorinated biphenyls (PCB) can be efficiently reduced in contaminated marine sediments through the reductive dechlorination (RD) process lead by anaerobic organohalide bacteria. Although the process has been extensively investigated on PCB-spiked sediments, the knowledge on the identity and metabolic potential of PCB-dechlorinating microorganisms in real contaminated matrix is still limited. Aim of this study was to explore the composition and the dynamics of the microbial communities of the marine sediment collected from one of the largest Sites of National Interest (SIN) in Italy (Mar Piccolo, Taranto) under conditions promoting the PCBs RD. A long-term microcosm study revealed that autochthonous bacteria were able to sustain the PCB dechlorination at a high extent and the successive addition of an external fermentable organic substrate (lactate) caused the further depletion of the high-chlorinated PCBs (up to 70%). Next Generation Sequencing was used to describe the core microbiome of the marine sediment and to follow the changes caused by the treatments. OTUs affiliated to sulfur-oxidizing ε-proteobacteria, Sulfurovum, and Sulfurimonas, were predominant in the original sediment and increased up to 60% of total OTUs after lactate addition. Other OTUs detected in the sediment were affiliated to sulfate reducing (δ-proteobacteria) and to organohalide respiring bacteria within Chloroflexi phylum mainly belonging to Dehalococcoidia class. Among others, Dehalococcoides mccartyi was enriched during the treatments even though the screening of the specific reductive dehalogenase genes revealed the occurrence of undescribed strains, which deserve further investigations. Overall, this study highlighted the potential of members of Dehalococcoidia class in reducing the contamination level of the marine sediment from Mar Piccolo with relevant implications on the selection of sustainable bioremediation strategies to clean-up the site.

Research paper thumbnail of First evidence on the occurrence and dynamics of Dehalococcoides mccartyi PCB-dechlorinase genes in marine sediment during Aroclor1254 reductive dechlorination (B. Matturro, M. Di Lenola, C. Ubaldi, S. Rossetti) —> Cite this article as Matturro et al., 2016

The present study evaluates the PCB-dehalorespiring capabilities and dynamics of indigenous Dehal... more The present study evaluates the PCB-dehalorespiring capabilities and dynamics of indigenous Dehalococcoides mccartyi population in a PCB contaminated marine sediment. Specialized PCB-dechlorinase genes pcbA1, pcbA4 and pcbA5 previously characterized in pure cultures of D. mccartyi, were here found for the first time in environmental samples. Reductive dechlorination was stimulated by spiking Aroclor1254 to the sediment and by imposing strictly anaerobic conditions both with and without bioaugmentation with a Dehalococcoides mccartyi enrichment culture. In line with the contaminant dechlorination kinetics, Dehalococcoides population increased during the entire incubation period showing growth yields of 4.94E + 07 Dehalococcoides per μmol Cl −1 and 7.30E + 05 Dehalococcoides per μmol Cl −1 in the marine sediment with and without bioaugmentation respectively. The pcbA4 and pcbA5 dechlorinase genes, and to a lesser extent pcbA1 gene, were enriched during the an-aerobic incubation suggesting their role in Aroclor1254 dechlorination under salinity conditions.

Research paper thumbnail of In situ detection of alkB2 gene involved in Alcanivorax borkumensis SK2 T hydrocarbon biodegradation. (Matturro B, Frascadore E, Cappello S, Genovese M, Rossetti S.) —> Cite this article as Matturro et al., 2016

This study aimed to develop a new assay based on the whole cell hybridization in order to monitor... more This study aimed to develop a new assay based on the whole cell hybridization in order to monitor alkane hy-droxylase genes (alkB system) of the marine bacterium Alcanivorax borkumensis SK2 T commonly reported as the predominant microorganism responsible for the biodegradation of n-alkanes which are the major fraction of petroleum hydrocarbons. The assay based on the whole cell hybridization targeting alkB2 gene was successfully developed and calibrated on a pure culture of Alcanivorax borkumensis SK2 T with a detection efficiency up to 80%. The approach was further successfully validated on hydrocarbon-contaminated seawater and provided cells abundance (6.74E+04 alkB2-carrying cells mL −1) higher of about one order of magnitude than those obtained by qPCR (4.96E+03 alkB2 gene copies mL −1). This study highlights the validity of the assay for the detection at single cell level of key-functional genes involved in the biodegradation of n-alkanes.

Research paper thumbnail of Reductive dechlorination of tetrachloroethene in marine sediments: Biodiversity and dehalorespiring capabilities of the indigenous microbes. (Matturro B, Presta E, Rossetti S.) —> Cite this article as Matturro et al., 2016

Chlorinated compounds pose environmental concerns due to their toxicity and wide distribution in ... more Chlorinated compounds pose environmental concerns due to their toxicity and wide distribution in several matrices. Microorganisms specialized in leading anaerobic reductive dechlorination (RD) processes, including Dehalococcoides mccartyi (Dhc), are able to reduce chlorinated compounds to harmless products or to less toxic forms. Here we report the first detailed study dealing with the RD potential of heavy polluted marine sediment by evaluating the biodegradation kinetics together with the composition, dynamics and activity of indigenous microbial population. A microcosm study was conducted under strictly anaerobic conditions on marine sediment collected near the marine coast of Sarno river mouth, one of the most polluted river in Europe. Tetrachloroethene (PCE), used as model pollutant, was completely converted to ethene within 150 days at reductive dechlorination rate equal to 0.016 meq L −1 d −1. Consecutive spikes of PCE allowed increasing the degradation kinetics up to 0.1 meq L −1 d −1 within 20 days. Strictly anaerobiosis and repeated spikes of PCE stimulated the growth of indigenous Dhc cells (growth yield of ~7.0E + 07 Dhc cells per μM Cl −1 released). Dhc strains carrying the reductive dehalogenase genes tceA and vcrA were detected in the original marine sediment and their number increased during the treatment as demonstrated by the high level of tceA expression at the end of the microcosm study (2.41E + 05 tceA gene transcripts g −1). Notably, the structure of the microbial communities was fully described by Catalysed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) as wells as the dynamics of the dechlorinating bacteria during the microcosms operation. Interestingly, a direct role of Dhc cells was ascertained

Research paper thumbnail of Polychlorinated biphenyl (PCB) anaerobic degradation in marine sediments: microcosm study and role of autochthonous microbial communities. (Matturro B, Ubaldi C, Grenni P, Caracciolo AB, Rossetti S.) —> Cite this article as Matturro et al., 2016

Polychlorobiphenyl (PCB) biodegradation was followed for 1 year in microcosms containing marine s... more Polychlorobiphenyl (PCB) biodegradation was followed for 1 year in microcosms containing marine sediments collected from Mar Piccolo (Taranto, Italy) chronically contaminated by this class of hazardous compounds. The microcosms were performed under strictly anaerobic conditions with or without the addition of Dehalococcoides mccartyi, the main microorganism known to degrade PCBs through the anaerobic reductive dechlorination process. Thirty PCB con-geners were monitored during the experiments revealing that the biodegradation occurred in all microcosms with a decrease in hepta-, hexa-, and penta-chlorobiphenyls (CBs) and a parallel increase in low chlorinated PCBs (tri-CBs and tetra-CBs). The concentrations of the most representative conge-ners detected in the original sediment, such as 245-245-CB and 2345-245-CB, and of the mixture 2356-34-CB+234-245-CB, decreased by 32.5, 23.8, and 46.7 %, respectively, after only 70 days of anaerobic incubation without any bioaugmen-tation treatment. Additionally, the structure and population dynamics of the microbial key players involved in the biodegradative process and of the entire mixed microbial community were accurately defined by Catalyzed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) in both the original sediment and during the operation of the microcosm. The reductive dehalogenase genes of D. mccartyi, specifically involved in PCB dechlorination, were also quantified using real-time PCR (qPCR). Our results demonstrated that the autochthonous microbial community living in the marine sediment, including D. mccartyi (6.32E+06 16S rRNA gene copy numbers g −1 sediment), was able to efficiently sustain the biodegradation of PCBs when controlled anaerobic conditions were imposed.

Research paper thumbnail of GeneCARD-FISH: Detection of tceA and vcrA reductive dehalogenase genes in Dehalococcoides mccartyi by fluorescence in situ hybridization (Matturro B, Rossetti S) —> Cite this article as Matturro et al., 2015

Journal of Microbiological Methods, 2015

Research paper thumbnail of Field distribution and activity of chlorinated solvents degrading bacteria by combining CARD-FISH and real time PCR

New Biotechnology, 2012

Nowadays several advanced molecular techniques are applied for quantifying bacteria involved in c... more Nowadays several advanced molecular techniques are applied for quantifying bacteria involved in contaminant degradation processes. However, despite the fact that significant efforts have been taken to make these tools more reliable and specific, their application for the analysis of field samples is hardly ever applied. In this study, a combination of three methods (CARD-FISH, qPCR and RT-qPCR) was successfully applied to evaluate the distribution and the activity of known chlorinated solvent dechlorinating bacteria in a contaminated site where no remedial actions have been undertaken. CAtalysed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) specifically provided the cell densities of known dechlorinating bacteria and was found to be more sensitive than quantitative PCR (qPCR) for the quantification of 'Dehalococcoides' cell numbers in the aquifer. Among the screened dechlorinators, 'Dehalococcoides' spp. were mainly found and nearly homogenously distributed in the aquifers at concentrations ranging from 8.1×10(5)±1.2×10(5) to 2.5×10(7)±5.6×10(6)cells per liter of groundwater (with a relative abundance out of the total Bacteria of 0.7-15%). Further, the dechlorination potentialities of 'Dehalococcoides' species living in the aquifer were evaluated by analyzing the abundance and the expression of 16S rRNA genes and reductive dehalogenase (RDase) encoding functional genes by qPCR and Reverse Transcription qPCR (RT-qPCR). 'Dehalococcoides'tceA gene, known to be associated to strains capable of reducing chlorinated solvents beyond cis-DCE, was found and expressed in the field. Overall, this study proved the existence of a well-established dechlorinating microbial community able to use contaminants as substrates for their metabolic activity and indicated the occurrence of reductive dechlorination at the site.

Research paper thumbnail of Reductive dechlorination of tetrachloroethene in marine sediments: Biodiversity and dehalorespiring capabilities of the indigenous microbes (B. Matturro, E. Presta, S. Rossetti) —> Cite this article as Matturro et al., 2016

Abstract Chlorinated compounds pose environmental concerns due to their toxicity and wide distrib... more Abstract
Chlorinated compounds pose environmental concerns due to their toxicity and wide distribution in several matrices. Microorganisms specialized in leading anaerobic reductive dechlorination (RD) processes, including Dehalococcoides mccartyi (Dhc), are able to reduce chlorinated compounds to harmless products or to less toxic forms. Here we report the first detailed study dealing with the RD potential of heavy polluted marine sediment by evaluating the biodegradation kinetics together with the composition, dynamics and activity of indigenous microbial population.
A microcosm study was conducted under strictly anaerobic conditions on marine sediment collected near the marine coast of Sarno river mouth, one of the most polluted river in Europe. Tetrachloroethene (PCE), used as model pollutant, was completely converted to ethene within 150 days at reductive dechlorination rate equal to 0.016 meq L-1 d-1. Consecutive spikes of PCE allowed increasing the degradation kinetics up to 0.1 meq L-1d-1 within 20 days. Strictly anaerobiosis and repeated spikes of PCE stimulated the growth of indigenous Dhc cells (growth yield of ~7.0E+07 Dhc cells per μM Cl-1 released). Dhc strains carrying the reductive dehalogenase genes tceA and vcrA were detected in the original marine sediment and their number increased during the treatment as demonstrated by the high level of tceA expression at the end of the microcosm study (2.41E+05 tceA gene transcripts g-1). Notably, the structure of the microbial communities was fully described by Catalysed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) as wells as the dynamics of the dechlorinating bacteria during the microcosms operation. Interestingly, a direct role of Dhc cells was ascertained suggesting the existence of strains adapted at salinity conditions. Additionally, non-Dhc Chloroflexi were retrieved in the original sediment and were kept stable over time suggesting their likely flanking role of the RD process.

Research paper thumbnail of Polychlorinated biphenyl (PCB) anaerobic degradation in marine sediments: microcosm study and role of autochthonous microbial communities. (Matturro B, Ubaldi C, Grenni P, Caracciolo AB, Rossetti S.)

Polychlorobiphenyl (PCB) biodegradation was followed for 1 year in microcosms containing marine s... more Polychlorobiphenyl (PCB) biodegradation was followed for 1 year in microcosms containing marine sediments collected from Mar Piccolo (Taranto, Italy) chronically contaminated by this class of hazardous compounds. The microcosms were performed under strictly anaerobic conditions with or without the addition of Dehalococcoides mccartyi, the main microorganism known to degrade PCBs through the anaerobic reductive dechlorination process. Thirty PCB congeners were monitored during the experiments revealing that the biodegradation occurred in all microcosms with a decrease in hepta-, hexa-, and penta-chlorobiphenyls (CBs) and a parallel increase in low chlorinated PCBs (tri-CBs and tetra-CBs). The concentrations of the most representative congeners detected in the original sediment, such as 245-245-CB and 2345-245-CB, and of the mixture 2356-34-CB+234-245-CB, decreased by 32.5, 23.8, and 46.7 %, respectively, after only 70 days of anaerobic incubation without any bioaugmentation treatment. Additionally, the structure and population dynamics of the microbial key players involved in the biodegradative process and of the entire mixed microbial community were accurately defined by Catalyzed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) in both the original sediment and during the operation of the microcosm. The reductive dehalogenase genes of D. mccartyi, specifically involved in PCB dechlorination, were also quantified using real-time PCR (qPCR). Our results demonstrated that the autochthonous microbial community living in the marine sediment, including D. mccartyi (6.32E+06 16S rRNA gene copy numbers g-1 sediment), was able to efficiently sustain the biodegradation of PCBs when controlled anaerobic conditions were imposed.

Research paper thumbnail of GeneCARD-FISH: Detection of tceA and vcrA reductive dehalogenase. (Bruna Matturro, Simona Rossetti)-> PLEASE CITE THIS ARTICLE AS "Matturro B. and Rossetti S., 2015" <-more

Due to the direct involvement in the biodegradation of chlorinated solvents, reductive dehalogena... more Due to the direct involvement in the biodegradation of chlorinated solvents, reductive dehalogenase genes (RDase) are considered biomarkers of the metabolic potential of different strains of Dehalococcoides mccartyi (Dhc). This is known to be the only microbe able to completely reduce toxic chlorinated solvents to harmless ethene. In the last years, several Molecular Biological Tools (MBTs) have been developed to optimize the detectability of Dhc cells and/or the RDase genes, with particular attention to the most important indicators of ethene formation, namely tceA and vcrA genes. Despite qPCR has been indicated as the MBT of choice, the use of CARD-FISH recently demonstrated to provide a more accurate quantification of Dhc cells in a wide concentration range, overcoming the drawbacks of loosing nucleic acids during the preparation of the sample associated with qPCR. CARD-FISH assays usually target 16S rRNA and up to date no protocol able to discriminate different Dhc strains by detecting RDase genes has been developed. This study reports the first evidence of in situ detection of tceA and vcrA genes into Dhc cells by applying a new procedure named geneCARD-FISH. Dhc strains carrying tceA and vcrA genes were identified and quantified in a PCE-to-ethene dechlorinating microbial enrichment and overall they represented 58.63% ± 2.45% and 40.46% ± 1.86% of the total Dhc cells, respectively. These values were markedly higher than those obtained by qPCR, which strongly underestimated the actual concentration of vcrA gene (0.08% ± 0.01% of Dhc 16S rRNA gene copies). The assay was successfully applied also for the analysis of environmental samples and remarkably strengthens the biomonitoring activities at field scale by providing the specific in situ discrimination of Dhc cells carrying the key-RDase genes.

Research paper thumbnail of Biomonitoraggio dei processi di degradazione di contaminanti in matrici ambientali. (Bruna Matturro, Simona Rossetti)

Research paper thumbnail of Bonifica di siti contaminati da solventi cloruarati: ruolo del biomonitoraggio (B. Matturro, S. Rossetti)

Rivista energia e ambiente , Sep 2013

Il monitoraggio ambientale mediante tecniche biomolecolari avanzate permette di identificare e qu... more Il monitoraggio ambientale mediante tecniche biomolecolari avanzate permette di identificare e quantificare in modo rapido e specifico i microorganismi coinvolti in processi di biodegradazione di contaminanti ai fini della valutazione predittiva del potenziale di biorisanamento di un sito inquinato.

Research paper thumbnail of Different activity levels of Dehalococcoides mccartyi revealed by FISH and CARD-FISH under non-steady and pseudo-steady state conditions (Matturro B., Tandoi V., Rossetti S. - 2013) -> PLEASE CITE THIS ARTICLE AS "Matturro et al., 2013" <-

Research paper thumbnail of Quantitative estimation of Dehalococcoides mccartyi at laboratory and field scale: comparative study between CARD-FISH and Real Time PCR (Matturro B., Heavner G. L., Richardson R.E., Rossetti S. - 2013) -> PLEASE CITE THIS ARTICLE AS "Matturro et al., 2013" <-

Journal of Microbiological Methods Volume 93, Issue 2, May 2013, Pages 127–133, May 2013

Dehalococcoides mccartyi detectability in the field is a fundamental tool to assess the efficienc... more Dehalococcoides mccartyi detectability in the field is a fundamental tool to assess the efficiency of natural attenuation or engineered bioremediation in chlorinated solvent-contaminated sites. This study reports on the direct comparison of quantitative data obtained by Real Time PCR (qPCR) and CAtalyzed Reporter Deposition-Fluorescence In situ Hybridization (CARD-FISH) over a wide range of Dehalococcoides concentrations (10–108 cells mL− 1) both in three independent 10-fold serial dilutions of a laboratory dechlorinating enrichment and in 49 groundwater samples from 6 different contaminated sites. Dehalococcoides enumeration by CARD-FISH yielded a linear curve in the analyzed concentration range which was consistent with the expected concentrations and showed good reproducibility in triplicate assays. Alternatively, qPCR did not allow for the discrimination of 16S rRNA gene concentrations lower than 103 gene copies mL− 1 either in the dechlorinating mixed culture or in field samples. Overall this study highlights the limits of qPCR quantification, especially in samples where low concentrations of this microorganism may be expected, and suggests the use of a confirmatory methodology under these particular conditions.CARD-FISH and qPCR were simultaneously applied to quantify Dehalococcoides mccartyi.The methods were applied on a PCE dechlorinating culture and field samples.Accuracy of CARD-FISH quantification over a large concentration range.qPCR yielded Dehalococcoides concentration lower than that obtained by CARD-FISH.qPCR failed to estimate 16SrRNA Dehalococcoides gene copies lower than 103 mL–1.

Research paper thumbnail of Predictive evaluation of bioremediation potential of chlorinated solvents contaminated sites (Matturro, B. and Rossetti, S. - 2012) -> PLEASE CITE THIS ARTICLE AS "Matturro et al., 2012" <-

""""The extent of chlorinated solvents biodegradation in contaminated aquifers is critically depe... more """"The extent of chlorinated solvents biodegradation in contaminated aquifers is critically dependent upon several factors, namely, the presence of contaminant degrading bacteria and the creation of optimal environmental conditions to stimulate biodegradative activity. The early estimation of the occurrence and abundance of contaminant key-degrading bacteria at field scale can therefore strongly assist the definition of the bioremediation potential as well as the decision making process prior to initiating bioremediation activities and the detailed site characterization. In this study, a wide screening was made on groundwater samples taken from several Italian chlorinated solvents contaminated sites to estimate the dechlorinators cell densities and activity. In particular, the analysis was made by means of a combination of biomolecular tools including in situ detection methods (CARD-FISH) for the accurate estimation of dechlorinators abundances and RT-qPCR for the expression analysis of reductive dehalogenase. Here, the outputs obtained by the application of the biomolecular tools were further compared with the results obtained by conventional treatability approach (i.e. microcosm study).
""""

Research paper thumbnail of Kinetics of reductive dechlorination of chlorinated ethenes: dynamics, abundance and activity of dechlorinating population. ( B. Matturro, M. Majone, F. Aulenta, V. Tandoi, S. Rossetti. - 2012) -> PLEASE CITE THIS ARTICLE AS "Matturro et al., 2012" <-

""""Three microbial cultures were enriched on PCE (hereafter named RM3), TCE (B2), or cis-DCE (B3... more """"Three microbial cultures were enriched on PCE (hereafter named RM3), TCE (B2), or cis-DCE (B3) (as electron acceptors) and lactate (as electron donor). All cultures, which were found to completely transform the chlorinated contaminants to ethene, were kinetically characterised and analysed to define the dynamics and activity of dechlorinating microbial populations. Specific biodegradation rates were estimated by means of conventional biomass parameter (i.e. VSS, volatile suspended solids) and by cell numbers of dechlorinating population specifically discriminated and quantified by biomolecular tools.
Kinetic batch experiments, carried out after a pseudo-steady state performance had been established, revealed that the observed dechlorination rates followed the order RM3 (PCE-enriched)>B2 (TCE-enriched)>B3 (cis-DCE-enriched).
Dechlorinating bacteria were quantified by combining in situ hybridization techniques and PCR-based approaches. Fluorescence in situ hybridization (FISH and CARD-FISH) allowed the cell numbers estimation of active and not active i) dechlorinating bacteria involved in the partial degradation of PCE or TCE to cis-DCE (Desulfitobacterium spp., Dehalobacter spp., spp., Geobacter spp., Sulfurospirillum spp.) as well as ii) “Dehalococcoides” spp. known to be able to completely dechlorinate such compounds to harmless ethene. Gene expression profile of reductive dehalogenase genes (tceA, bvcA, vcrA) were also estimated and correlated to kinetic performances and dechlorinating bacteria abundances. The potential for field application of the outputs of this study will be discussed.""""

Research paper thumbnail of Biomonitoring of Chlorinated Solvents-Degrading Bacteria In Contaminated Soil and Groundwater (Simona Rossetti, Bruna Matturro, Federico Aulenta, Francesco Valentino, Marco Petrangeli Papini, Valter Tandoi. - 2010) --> PLEASE CITE THIS ARTICLE AS "Rossetti et al., 2012"

Journal of Biotechnology, Jan 1, 2010

Research paper thumbnail of Applicazione delle tecniche biomolecolari per la caratterizzazione e il monitoraggio di siti contaminati da solventi clorurati. Biomonitoraggio di siti contaminati da solventi clorurati. (B. Matturro) -> PLEASE CITE THIS ARTICLE AS "Matturro B., 2009" <-

Teaching Documents by Bruna Matturro

Research paper thumbnail of Biomonitoraggio dei processi di degradazione di contaminanti in matrici ambientali. 2013. (Rossetti S., Matturro B.) --> PLEASE CITE THIS ARTICLE AS "Rossetti et al., 2012"

Research paper thumbnail of Cable Bacteria and the Bioelectrochemical Snorkel: The Natural and Engineered Facets Playing a Role in Hydrocarbons Degradation in Marine Sediments (Bruna Matturro, Carolina Cruz Viggi, Federico Aulenta and Simona Rossetti) —> Please cite this article as Matturro et al., 2017

The composition and metabolic traits of the microbial communities acting in an innovative bioelec... more The composition and metabolic traits of the microbial communities acting in an innovative bioelectrochemical system were here investigated. The system, known as Oil Spill Snorkel, was recently developed to stimulate the oxidative biodegradation of petroleum hydrocarbons in anoxic marine sediments. Next Generation Sequencing was used to describe the microbiome of the bulk sediment and of the biofilm growing attached to the surface of the electrode. The analysis revealed that sulfur cycling primarily drives the microbial metabolic activities occurring in the bioelectrochemical system. In the anoxic zone of the contaminated marine sediment, petroleum hydrocarbon degradation occurred under sulfate-reducing conditions and was lead by different families of Desulfobacterales (46% of total OTUs). Remarkably, the occurrence of filamentous Desulfubulbaceae, known to be capable to vehicle electrons deriving from sulfide oxidation to oxygen serving as a spatially distant electron acceptor, was demonstrated. Differently from the sediment, which was mostly colonized by Deltaproteobacteria, the biofilm at the anode hosted, at high extent, members of Alphaproteobacteria (59%) mostly affiliated to Rhodospirillaceae family (33%) and including several known sulfur-and sulfide-oxidizing genera. Overall, we showed the occurrence in the system of a variety of electroactive microorganisms able to sustain the contaminant biodegradation alone or by means of an external conductive support through the establishment of a bioelectrochemical connection between two spatially separated redox zones and the preservation of an efficient sulfur cycling.

Research paper thumbnail of Microbiome Dynamics of a Polychlorobiphenyl (PCB) Historically Contaminated Marine Sediment under Conditions Promoting Reductive Dechlorination (B. Matturro, C. Ubaldi, S. Rossetti) —> Please cite this article as Matturro et al., 2016

The toxicity of polychlorinated biphenyls (PCB) can be efficiently reduced in contaminated marine... more The toxicity of polychlorinated biphenyls (PCB) can be efficiently reduced in contaminated marine sediments through the reductive dechlorination (RD) process lead by anaerobic organohalide bacteria. Although the process has been extensively investigated on PCB-spiked sediments, the knowledge on the identity and metabolic potential of PCB-dechlorinating microorganisms in real contaminated matrix is still limited. Aim of this study was to explore the composition and the dynamics of the microbial communities of the marine sediment collected from one of the largest Sites of National Interest (SIN) in Italy (Mar Piccolo, Taranto) under conditions promoting the PCBs RD. A long-term microcosm study revealed that autochthonous bacteria were able to sustain the PCB dechlorination at a high extent and the successive addition of an external fermentable organic substrate (lactate) caused the further depletion of the high-chlorinated PCBs (up to 70%). Next Generation Sequencing was used to describe the core microbiome of the marine sediment and to follow the changes caused by the treatments. OTUs affiliated to sulfur-oxidizing ε-proteobacteria, Sulfurovum, and Sulfurimonas, were predominant in the original sediment and increased up to 60% of total OTUs after lactate addition. Other OTUs detected in the sediment were affiliated to sulfate reducing (δ-proteobacteria) and to organohalide respiring bacteria within Chloroflexi phylum mainly belonging to Dehalococcoidia class. Among others, Dehalococcoides mccartyi was enriched during the treatments even though the screening of the specific reductive dehalogenase genes revealed the occurrence of undescribed strains, which deserve further investigations. Overall, this study highlighted the potential of members of Dehalococcoidia class in reducing the contamination level of the marine sediment from Mar Piccolo with relevant implications on the selection of sustainable bioremediation strategies to clean-up the site.

Research paper thumbnail of First evidence on the occurrence and dynamics of Dehalococcoides mccartyi PCB-dechlorinase genes in marine sediment during Aroclor1254 reductive dechlorination (B. Matturro, M. Di Lenola, C. Ubaldi, S. Rossetti) —> Cite this article as Matturro et al., 2016

The present study evaluates the PCB-dehalorespiring capabilities and dynamics of indigenous Dehal... more The present study evaluates the PCB-dehalorespiring capabilities and dynamics of indigenous Dehalococcoides mccartyi population in a PCB contaminated marine sediment. Specialized PCB-dechlorinase genes pcbA1, pcbA4 and pcbA5 previously characterized in pure cultures of D. mccartyi, were here found for the first time in environmental samples. Reductive dechlorination was stimulated by spiking Aroclor1254 to the sediment and by imposing strictly anaerobic conditions both with and without bioaugmentation with a Dehalococcoides mccartyi enrichment culture. In line with the contaminant dechlorination kinetics, Dehalococcoides population increased during the entire incubation period showing growth yields of 4.94E + 07 Dehalococcoides per μmol Cl −1 and 7.30E + 05 Dehalococcoides per μmol Cl −1 in the marine sediment with and without bioaugmentation respectively. The pcbA4 and pcbA5 dechlorinase genes, and to a lesser extent pcbA1 gene, were enriched during the an-aerobic incubation suggesting their role in Aroclor1254 dechlorination under salinity conditions.

Research paper thumbnail of In situ detection of alkB2 gene involved in Alcanivorax borkumensis SK2 T hydrocarbon biodegradation. (Matturro B, Frascadore E, Cappello S, Genovese M, Rossetti S.) —> Cite this article as Matturro et al., 2016

This study aimed to develop a new assay based on the whole cell hybridization in order to monitor... more This study aimed to develop a new assay based on the whole cell hybridization in order to monitor alkane hy-droxylase genes (alkB system) of the marine bacterium Alcanivorax borkumensis SK2 T commonly reported as the predominant microorganism responsible for the biodegradation of n-alkanes which are the major fraction of petroleum hydrocarbons. The assay based on the whole cell hybridization targeting alkB2 gene was successfully developed and calibrated on a pure culture of Alcanivorax borkumensis SK2 T with a detection efficiency up to 80%. The approach was further successfully validated on hydrocarbon-contaminated seawater and provided cells abundance (6.74E+04 alkB2-carrying cells mL −1) higher of about one order of magnitude than those obtained by qPCR (4.96E+03 alkB2 gene copies mL −1). This study highlights the validity of the assay for the detection at single cell level of key-functional genes involved in the biodegradation of n-alkanes.

Research paper thumbnail of Reductive dechlorination of tetrachloroethene in marine sediments: Biodiversity and dehalorespiring capabilities of the indigenous microbes. (Matturro B, Presta E, Rossetti S.) —> Cite this article as Matturro et al., 2016

Chlorinated compounds pose environmental concerns due to their toxicity and wide distribution in ... more Chlorinated compounds pose environmental concerns due to their toxicity and wide distribution in several matrices. Microorganisms specialized in leading anaerobic reductive dechlorination (RD) processes, including Dehalococcoides mccartyi (Dhc), are able to reduce chlorinated compounds to harmless products or to less toxic forms. Here we report the first detailed study dealing with the RD potential of heavy polluted marine sediment by evaluating the biodegradation kinetics together with the composition, dynamics and activity of indigenous microbial population. A microcosm study was conducted under strictly anaerobic conditions on marine sediment collected near the marine coast of Sarno river mouth, one of the most polluted river in Europe. Tetrachloroethene (PCE), used as model pollutant, was completely converted to ethene within 150 days at reductive dechlorination rate equal to 0.016 meq L −1 d −1. Consecutive spikes of PCE allowed increasing the degradation kinetics up to 0.1 meq L −1 d −1 within 20 days. Strictly anaerobiosis and repeated spikes of PCE stimulated the growth of indigenous Dhc cells (growth yield of ~7.0E + 07 Dhc cells per μM Cl −1 released). Dhc strains carrying the reductive dehalogenase genes tceA and vcrA were detected in the original marine sediment and their number increased during the treatment as demonstrated by the high level of tceA expression at the end of the microcosm study (2.41E + 05 tceA gene transcripts g −1). Notably, the structure of the microbial communities was fully described by Catalysed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) as wells as the dynamics of the dechlorinating bacteria during the microcosms operation. Interestingly, a direct role of Dhc cells was ascertained

Research paper thumbnail of Polychlorinated biphenyl (PCB) anaerobic degradation in marine sediments: microcosm study and role of autochthonous microbial communities. (Matturro B, Ubaldi C, Grenni P, Caracciolo AB, Rossetti S.) —> Cite this article as Matturro et al., 2016

Polychlorobiphenyl (PCB) biodegradation was followed for 1 year in microcosms containing marine s... more Polychlorobiphenyl (PCB) biodegradation was followed for 1 year in microcosms containing marine sediments collected from Mar Piccolo (Taranto, Italy) chronically contaminated by this class of hazardous compounds. The microcosms were performed under strictly anaerobic conditions with or without the addition of Dehalococcoides mccartyi, the main microorganism known to degrade PCBs through the anaerobic reductive dechlorination process. Thirty PCB con-geners were monitored during the experiments revealing that the biodegradation occurred in all microcosms with a decrease in hepta-, hexa-, and penta-chlorobiphenyls (CBs) and a parallel increase in low chlorinated PCBs (tri-CBs and tetra-CBs). The concentrations of the most representative conge-ners detected in the original sediment, such as 245-245-CB and 2345-245-CB, and of the mixture 2356-34-CB+234-245-CB, decreased by 32.5, 23.8, and 46.7 %, respectively, after only 70 days of anaerobic incubation without any bioaugmen-tation treatment. Additionally, the structure and population dynamics of the microbial key players involved in the biodegradative process and of the entire mixed microbial community were accurately defined by Catalyzed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) in both the original sediment and during the operation of the microcosm. The reductive dehalogenase genes of D. mccartyi, specifically involved in PCB dechlorination, were also quantified using real-time PCR (qPCR). Our results demonstrated that the autochthonous microbial community living in the marine sediment, including D. mccartyi (6.32E+06 16S rRNA gene copy numbers g −1 sediment), was able to efficiently sustain the biodegradation of PCBs when controlled anaerobic conditions were imposed.

Research paper thumbnail of GeneCARD-FISH: Detection of tceA and vcrA reductive dehalogenase genes in Dehalococcoides mccartyi by fluorescence in situ hybridization (Matturro B, Rossetti S) —> Cite this article as Matturro et al., 2015

Journal of Microbiological Methods, 2015

Research paper thumbnail of Field distribution and activity of chlorinated solvents degrading bacteria by combining CARD-FISH and real time PCR

New Biotechnology, 2012

Nowadays several advanced molecular techniques are applied for quantifying bacteria involved in c... more Nowadays several advanced molecular techniques are applied for quantifying bacteria involved in contaminant degradation processes. However, despite the fact that significant efforts have been taken to make these tools more reliable and specific, their application for the analysis of field samples is hardly ever applied. In this study, a combination of three methods (CARD-FISH, qPCR and RT-qPCR) was successfully applied to evaluate the distribution and the activity of known chlorinated solvent dechlorinating bacteria in a contaminated site where no remedial actions have been undertaken. CAtalysed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) specifically provided the cell densities of known dechlorinating bacteria and was found to be more sensitive than quantitative PCR (qPCR) for the quantification of &#39;Dehalococcoides&#39; cell numbers in the aquifer. Among the screened dechlorinators, &#39;Dehalococcoides&#39; spp. were mainly found and nearly homogenously distributed in the aquifers at concentrations ranging from 8.1×10(5)±1.2×10(5) to 2.5×10(7)±5.6×10(6)cells per liter of groundwater (with a relative abundance out of the total Bacteria of 0.7-15%). Further, the dechlorination potentialities of &#39;Dehalococcoides&#39; species living in the aquifer were evaluated by analyzing the abundance and the expression of 16S rRNA genes and reductive dehalogenase (RDase) encoding functional genes by qPCR and Reverse Transcription qPCR (RT-qPCR). &#39;Dehalococcoides&#39;tceA gene, known to be associated to strains capable of reducing chlorinated solvents beyond cis-DCE, was found and expressed in the field. Overall, this study proved the existence of a well-established dechlorinating microbial community able to use contaminants as substrates for their metabolic activity and indicated the occurrence of reductive dechlorination at the site.

Research paper thumbnail of Reductive dechlorination of tetrachloroethene in marine sediments: Biodiversity and dehalorespiring capabilities of the indigenous microbes (B. Matturro, E. Presta, S. Rossetti) —> Cite this article as Matturro et al., 2016

Abstract Chlorinated compounds pose environmental concerns due to their toxicity and wide distrib... more Abstract
Chlorinated compounds pose environmental concerns due to their toxicity and wide distribution in several matrices. Microorganisms specialized in leading anaerobic reductive dechlorination (RD) processes, including Dehalococcoides mccartyi (Dhc), are able to reduce chlorinated compounds to harmless products or to less toxic forms. Here we report the first detailed study dealing with the RD potential of heavy polluted marine sediment by evaluating the biodegradation kinetics together with the composition, dynamics and activity of indigenous microbial population.
A microcosm study was conducted under strictly anaerobic conditions on marine sediment collected near the marine coast of Sarno river mouth, one of the most polluted river in Europe. Tetrachloroethene (PCE), used as model pollutant, was completely converted to ethene within 150 days at reductive dechlorination rate equal to 0.016 meq L-1 d-1. Consecutive spikes of PCE allowed increasing the degradation kinetics up to 0.1 meq L-1d-1 within 20 days. Strictly anaerobiosis and repeated spikes of PCE stimulated the growth of indigenous Dhc cells (growth yield of ~7.0E+07 Dhc cells per μM Cl-1 released). Dhc strains carrying the reductive dehalogenase genes tceA and vcrA were detected in the original marine sediment and their number increased during the treatment as demonstrated by the high level of tceA expression at the end of the microcosm study (2.41E+05 tceA gene transcripts g-1). Notably, the structure of the microbial communities was fully described by Catalysed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) as wells as the dynamics of the dechlorinating bacteria during the microcosms operation. Interestingly, a direct role of Dhc cells was ascertained suggesting the existence of strains adapted at salinity conditions. Additionally, non-Dhc Chloroflexi were retrieved in the original sediment and were kept stable over time suggesting their likely flanking role of the RD process.

Research paper thumbnail of Polychlorinated biphenyl (PCB) anaerobic degradation in marine sediments: microcosm study and role of autochthonous microbial communities. (Matturro B, Ubaldi C, Grenni P, Caracciolo AB, Rossetti S.)

Polychlorobiphenyl (PCB) biodegradation was followed for 1 year in microcosms containing marine s... more Polychlorobiphenyl (PCB) biodegradation was followed for 1 year in microcosms containing marine sediments collected from Mar Piccolo (Taranto, Italy) chronically contaminated by this class of hazardous compounds. The microcosms were performed under strictly anaerobic conditions with or without the addition of Dehalococcoides mccartyi, the main microorganism known to degrade PCBs through the anaerobic reductive dechlorination process. Thirty PCB congeners were monitored during the experiments revealing that the biodegradation occurred in all microcosms with a decrease in hepta-, hexa-, and penta-chlorobiphenyls (CBs) and a parallel increase in low chlorinated PCBs (tri-CBs and tetra-CBs). The concentrations of the most representative congeners detected in the original sediment, such as 245-245-CB and 2345-245-CB, and of the mixture 2356-34-CB+234-245-CB, decreased by 32.5, 23.8, and 46.7 %, respectively, after only 70 days of anaerobic incubation without any bioaugmentation treatment. Additionally, the structure and population dynamics of the microbial key players involved in the biodegradative process and of the entire mixed microbial community were accurately defined by Catalyzed Reporter Deposition Fluorescence In Situ Hybridization (CARD-FISH) in both the original sediment and during the operation of the microcosm. The reductive dehalogenase genes of D. mccartyi, specifically involved in PCB dechlorination, were also quantified using real-time PCR (qPCR). Our results demonstrated that the autochthonous microbial community living in the marine sediment, including D. mccartyi (6.32E+06 16S rRNA gene copy numbers g-1 sediment), was able to efficiently sustain the biodegradation of PCBs when controlled anaerobic conditions were imposed.

Research paper thumbnail of GeneCARD-FISH: Detection of tceA and vcrA reductive dehalogenase. (Bruna Matturro, Simona Rossetti)-> PLEASE CITE THIS ARTICLE AS "Matturro B. and Rossetti S., 2015" <-more

Due to the direct involvement in the biodegradation of chlorinated solvents, reductive dehalogena... more Due to the direct involvement in the biodegradation of chlorinated solvents, reductive dehalogenase genes (RDase) are considered biomarkers of the metabolic potential of different strains of Dehalococcoides mccartyi (Dhc). This is known to be the only microbe able to completely reduce toxic chlorinated solvents to harmless ethene. In the last years, several Molecular Biological Tools (MBTs) have been developed to optimize the detectability of Dhc cells and/or the RDase genes, with particular attention to the most important indicators of ethene formation, namely tceA and vcrA genes. Despite qPCR has been indicated as the MBT of choice, the use of CARD-FISH recently demonstrated to provide a more accurate quantification of Dhc cells in a wide concentration range, overcoming the drawbacks of loosing nucleic acids during the preparation of the sample associated with qPCR. CARD-FISH assays usually target 16S rRNA and up to date no protocol able to discriminate different Dhc strains by detecting RDase genes has been developed. This study reports the first evidence of in situ detection of tceA and vcrA genes into Dhc cells by applying a new procedure named geneCARD-FISH. Dhc strains carrying tceA and vcrA genes were identified and quantified in a PCE-to-ethene dechlorinating microbial enrichment and overall they represented 58.63% ± 2.45% and 40.46% ± 1.86% of the total Dhc cells, respectively. These values were markedly higher than those obtained by qPCR, which strongly underestimated the actual concentration of vcrA gene (0.08% ± 0.01% of Dhc 16S rRNA gene copies). The assay was successfully applied also for the analysis of environmental samples and remarkably strengthens the biomonitoring activities at field scale by providing the specific in situ discrimination of Dhc cells carrying the key-RDase genes.

Research paper thumbnail of Biomonitoraggio dei processi di degradazione di contaminanti in matrici ambientali. (Bruna Matturro, Simona Rossetti)

Research paper thumbnail of Bonifica di siti contaminati da solventi cloruarati: ruolo del biomonitoraggio (B. Matturro, S. Rossetti)

Rivista energia e ambiente , Sep 2013

Il monitoraggio ambientale mediante tecniche biomolecolari avanzate permette di identificare e qu... more Il monitoraggio ambientale mediante tecniche biomolecolari avanzate permette di identificare e quantificare in modo rapido e specifico i microorganismi coinvolti in processi di biodegradazione di contaminanti ai fini della valutazione predittiva del potenziale di biorisanamento di un sito inquinato.

Research paper thumbnail of Different activity levels of Dehalococcoides mccartyi revealed by FISH and CARD-FISH under non-steady and pseudo-steady state conditions (Matturro B., Tandoi V., Rossetti S. - 2013) -> PLEASE CITE THIS ARTICLE AS "Matturro et al., 2013" <-

Research paper thumbnail of Quantitative estimation of Dehalococcoides mccartyi at laboratory and field scale: comparative study between CARD-FISH and Real Time PCR (Matturro B., Heavner G. L., Richardson R.E., Rossetti S. - 2013) -> PLEASE CITE THIS ARTICLE AS "Matturro et al., 2013" <-

Journal of Microbiological Methods Volume 93, Issue 2, May 2013, Pages 127–133, May 2013

Dehalococcoides mccartyi detectability in the field is a fundamental tool to assess the efficienc... more Dehalococcoides mccartyi detectability in the field is a fundamental tool to assess the efficiency of natural attenuation or engineered bioremediation in chlorinated solvent-contaminated sites. This study reports on the direct comparison of quantitative data obtained by Real Time PCR (qPCR) and CAtalyzed Reporter Deposition-Fluorescence In situ Hybridization (CARD-FISH) over a wide range of Dehalococcoides concentrations (10–108 cells mL− 1) both in three independent 10-fold serial dilutions of a laboratory dechlorinating enrichment and in 49 groundwater samples from 6 different contaminated sites. Dehalococcoides enumeration by CARD-FISH yielded a linear curve in the analyzed concentration range which was consistent with the expected concentrations and showed good reproducibility in triplicate assays. Alternatively, qPCR did not allow for the discrimination of 16S rRNA gene concentrations lower than 103 gene copies mL− 1 either in the dechlorinating mixed culture or in field samples. Overall this study highlights the limits of qPCR quantification, especially in samples where low concentrations of this microorganism may be expected, and suggests the use of a confirmatory methodology under these particular conditions.CARD-FISH and qPCR were simultaneously applied to quantify Dehalococcoides mccartyi.The methods were applied on a PCE dechlorinating culture and field samples.Accuracy of CARD-FISH quantification over a large concentration range.qPCR yielded Dehalococcoides concentration lower than that obtained by CARD-FISH.qPCR failed to estimate 16SrRNA Dehalococcoides gene copies lower than 103 mL–1.

Research paper thumbnail of Predictive evaluation of bioremediation potential of chlorinated solvents contaminated sites (Matturro, B. and Rossetti, S. - 2012) -> PLEASE CITE THIS ARTICLE AS "Matturro et al., 2012" <-

""""The extent of chlorinated solvents biodegradation in contaminated aquifers is critically depe... more """"The extent of chlorinated solvents biodegradation in contaminated aquifers is critically dependent upon several factors, namely, the presence of contaminant degrading bacteria and the creation of optimal environmental conditions to stimulate biodegradative activity. The early estimation of the occurrence and abundance of contaminant key-degrading bacteria at field scale can therefore strongly assist the definition of the bioremediation potential as well as the decision making process prior to initiating bioremediation activities and the detailed site characterization. In this study, a wide screening was made on groundwater samples taken from several Italian chlorinated solvents contaminated sites to estimate the dechlorinators cell densities and activity. In particular, the analysis was made by means of a combination of biomolecular tools including in situ detection methods (CARD-FISH) for the accurate estimation of dechlorinators abundances and RT-qPCR for the expression analysis of reductive dehalogenase. Here, the outputs obtained by the application of the biomolecular tools were further compared with the results obtained by conventional treatability approach (i.e. microcosm study).
""""

Research paper thumbnail of Kinetics of reductive dechlorination of chlorinated ethenes: dynamics, abundance and activity of dechlorinating population. ( B. Matturro, M. Majone, F. Aulenta, V. Tandoi, S. Rossetti. - 2012) -> PLEASE CITE THIS ARTICLE AS "Matturro et al., 2012" <-

""""Three microbial cultures were enriched on PCE (hereafter named RM3), TCE (B2), or cis-DCE (B3... more """"Three microbial cultures were enriched on PCE (hereafter named RM3), TCE (B2), or cis-DCE (B3) (as electron acceptors) and lactate (as electron donor). All cultures, which were found to completely transform the chlorinated contaminants to ethene, were kinetically characterised and analysed to define the dynamics and activity of dechlorinating microbial populations. Specific biodegradation rates were estimated by means of conventional biomass parameter (i.e. VSS, volatile suspended solids) and by cell numbers of dechlorinating population specifically discriminated and quantified by biomolecular tools.
Kinetic batch experiments, carried out after a pseudo-steady state performance had been established, revealed that the observed dechlorination rates followed the order RM3 (PCE-enriched)>B2 (TCE-enriched)>B3 (cis-DCE-enriched).
Dechlorinating bacteria were quantified by combining in situ hybridization techniques and PCR-based approaches. Fluorescence in situ hybridization (FISH and CARD-FISH) allowed the cell numbers estimation of active and not active i) dechlorinating bacteria involved in the partial degradation of PCE or TCE to cis-DCE (Desulfitobacterium spp., Dehalobacter spp., spp., Geobacter spp., Sulfurospirillum spp.) as well as ii) “Dehalococcoides” spp. known to be able to completely dechlorinate such compounds to harmless ethene. Gene expression profile of reductive dehalogenase genes (tceA, bvcA, vcrA) were also estimated and correlated to kinetic performances and dechlorinating bacteria abundances. The potential for field application of the outputs of this study will be discussed.""""

Research paper thumbnail of Biomonitoring of Chlorinated Solvents-Degrading Bacteria In Contaminated Soil and Groundwater (Simona Rossetti, Bruna Matturro, Federico Aulenta, Francesco Valentino, Marco Petrangeli Papini, Valter Tandoi. - 2010) --> PLEASE CITE THIS ARTICLE AS "Rossetti et al., 2012"

Journal of Biotechnology, Jan 1, 2010

Research paper thumbnail of Applicazione delle tecniche biomolecolari per la caratterizzazione e il monitoraggio di siti contaminati da solventi clorurati. Biomonitoraggio di siti contaminati da solventi clorurati. (B. Matturro) -> PLEASE CITE THIS ARTICLE AS "Matturro B., 2009" <-

Research paper thumbnail of Biomonitoraggio dei processi di degradazione di contaminanti in matrici ambientali. 2013. (Rossetti S., Matturro B.) --> PLEASE CITE THIS ARTICLE AS "Rossetti et al., 2012"

Research paper thumbnail of Training course: Contaminated site remediation - Application of advanced tools to control biological processes

Lecturers and tutors Simona Rossetti, IRSA-CNR, Italy Anna Barra Caracciolo, IRSA-CNR, Italy F... more Lecturers and tutors
Simona Rossetti, IRSA-CNR, Italy
Anna Barra Caracciolo, IRSA-CNR, Italy
Federico Aulenta, IRSA-CNR, Italy
Claudia Beimfohr, Vermicon, Germany
Philippe Corvini , University of Applied Sciences and Arts
Northwestern Switzerland, Switzerland
Paola Grenni, IRSA-CNR, Italy
Nicolas Kalogerakis, Technical University of Crete, Greece
Caterina Levantesi, IRSA-CNR, Italy
Mauro Majone, Sapienza University of Rome, Italy
Bruna Matturro, IRSA-CNR, Italy
Jochen Müller, UFZ, Germany
Maurizio Petruccioli, DIBAF, University of Tuscia, Italy
Valter Tandoi, IRSA-CNR, Italy
Paul Wilmes, University of Luxembourg, Luxembourg
Giulio Zanaroli, University of Bologna, Italy""