Leandro F Estrozi | Centre National de la Recherche Scientifique / French National Centre for Scientific Research (original) (raw)

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Papers by Leandro F Estrozi

Research paper thumbnail of Fast projection matching for cryo-electron microscopy image reconstruction

Journal of Structural Biology, 2008

Research paper thumbnail of Ab initio high-resolution single-particle 3D reconstructions: The symmetry adapted functions way

Journal of Structural Biology, 2010

Research paper thumbnail of Multiresolution shape representation without border shifting

Electronics Letters, 1999

ABSTRACT The authors show how exact dilations can be used to obtain multiresolution skeletons and... more ABSTRACT The authors show how exact dilations can be used to obtain multiresolution skeletons and how a new multiresolution approach to shape representation can be achieved in terms of such skeletons. In addition to allowing the accurate reconstruction of multiresolution versions of the original binary shapes, the proposed approach is characterised by great simplicity and the important property that the shape borders are not shifted as the smoothing increases

Research paper thumbnail of Geometric Mismatches within the Concentric Layers of Rotavirus Particles: a Potential Regulatory Switch of Viral Particle Transcription Activity

Journal of Virology, 2008

Research paper thumbnail of 1D and 2D Fourier-based approaches to numeric curvature estimation and their comparative performance assessment

Digital Signal Processing, 2003

Research paper thumbnail of On voronoi diagrams and medial axes

Research paper thumbnail of Structure determination of feline calicivirus virus-like particles in the context of a pseudo-octahedral arrangement

PloS one, 2015

The vesivirus feline calicivirus (FCV) is a positive strand RNA virus encapsidated by an icosahed... more The vesivirus feline calicivirus (FCV) is a positive strand RNA virus encapsidated by an icosahedral T=3 shell formed by the viral VP1 protein. Upon its expression in the insect cell - baculovirus system in the context of vaccine development, two types of virus-like particles (VLPs) were formed, a majority built of 60 subunits (T=1) and a minority probably built of 180 subunits (T=3). The structure of the small particles was determined by x-ray crystallography at 0.8 nm resolution helped by cryo-electron microscopy in order to understand their formation. Cubic crystals belonged to space group P213. Their self-rotation function showed the presence of an octahedral pseudo-symmetry similar to the one described previously by Agerbandje and co-workers for human parvovirus VLPs. The crystal structure could be solved starting from the published VP1 structure in the context of the T=3 viral capsid. In contrast to viral capsids, where the capsomers are interlocked by the exchange of the N-te...

Research paper thumbnail of Structural Similarity of Secretins from Type II and Type III Secretion Systems

Structure, 2014

Secretins, the outer membrane components of several secretion systems in Gram-negative bacteria, ... more Secretins, the outer membrane components of several secretion systems in Gram-negative bacteria, assemble into channels that allow exoproteins to traverse the membrane. The membrane-inserted, multimeric regions of PscC, the Pseudomonas aeruginosa type III secretion system secretin, and PulD, the Klebsiella oxytoca type II secretion system secretin, were purified after cell-free synthesis and their structures analyzed by single particle cryoelectron microscopy. Both homomultimeric, barrel-like structures display a "cup and saucer" architecture. The "saucer" region of both secretins is composed of two distinct rings, with that of PulD being less segmented than that of PscC. Both secretins have a central chamber that is occluded by a plug linked to the chamber walls through hairpin-like structures. Comparisons with published structures from other bacterial systems reveal that secretins have regions of local structural flexibility, probably reflecting their evolved functions in protein secretion and needle assembly.

Research paper thumbnail of A Biologically-Motivated Approach to Image Representation and Its Application to Neuromorphology

Lecture Notes in Computer Science, 2000

Research paper thumbnail of Phasing of the Triatoma virus diffraction data using a cryo-electron microscopy reconstruction

Research paper thumbnail of Cryo-EM structure of the E. coli translating ribosome in complex with SRP and its receptor

Nature Structural & Molecular Biology, 2011

Research paper thumbnail of Three-Dimensional Structure of Canine Adenovirus Serotype 2 Capsid

Journal of Virology, 2008

Research paper thumbnail of SCA: Symmetry-based center assignment of 2D projections of symmetric 3D objects

Journal of Structural Biology, 2007

Research paper thumbnail of The cryo-EM Reconstruction of Drosophila C Virus (DCV) at 5.4 Å

Biophysical Journal, 2013

Research paper thumbnail of Oligomerization paths of the nucleoprotein of influenza A virus

Research paper thumbnail of Fast projection matching for cryo-electron microscopy image reconstruction

Journal of Structural Biology, 2008

Research paper thumbnail of Ab initio high-resolution single-particle 3D reconstructions: The symmetry adapted functions way

Journal of Structural Biology, 2010

Research paper thumbnail of Multiresolution shape representation without border shifting

Electronics Letters, 1999

ABSTRACT The authors show how exact dilations can be used to obtain multiresolution skeletons and... more ABSTRACT The authors show how exact dilations can be used to obtain multiresolution skeletons and how a new multiresolution approach to shape representation can be achieved in terms of such skeletons. In addition to allowing the accurate reconstruction of multiresolution versions of the original binary shapes, the proposed approach is characterised by great simplicity and the important property that the shape borders are not shifted as the smoothing increases

Research paper thumbnail of Geometric Mismatches within the Concentric Layers of Rotavirus Particles: a Potential Regulatory Switch of Viral Particle Transcription Activity

Journal of Virology, 2008

Research paper thumbnail of 1D and 2D Fourier-based approaches to numeric curvature estimation and their comparative performance assessment

Digital Signal Processing, 2003

Research paper thumbnail of On voronoi diagrams and medial axes

Research paper thumbnail of Structure determination of feline calicivirus virus-like particles in the context of a pseudo-octahedral arrangement

PloS one, 2015

The vesivirus feline calicivirus (FCV) is a positive strand RNA virus encapsidated by an icosahed... more The vesivirus feline calicivirus (FCV) is a positive strand RNA virus encapsidated by an icosahedral T=3 shell formed by the viral VP1 protein. Upon its expression in the insect cell - baculovirus system in the context of vaccine development, two types of virus-like particles (VLPs) were formed, a majority built of 60 subunits (T=1) and a minority probably built of 180 subunits (T=3). The structure of the small particles was determined by x-ray crystallography at 0.8 nm resolution helped by cryo-electron microscopy in order to understand their formation. Cubic crystals belonged to space group P213. Their self-rotation function showed the presence of an octahedral pseudo-symmetry similar to the one described previously by Agerbandje and co-workers for human parvovirus VLPs. The crystal structure could be solved starting from the published VP1 structure in the context of the T=3 viral capsid. In contrast to viral capsids, where the capsomers are interlocked by the exchange of the N-te...

Research paper thumbnail of Structural Similarity of Secretins from Type II and Type III Secretion Systems

Structure, 2014

Secretins, the outer membrane components of several secretion systems in Gram-negative bacteria, ... more Secretins, the outer membrane components of several secretion systems in Gram-negative bacteria, assemble into channels that allow exoproteins to traverse the membrane. The membrane-inserted, multimeric regions of PscC, the Pseudomonas aeruginosa type III secretion system secretin, and PulD, the Klebsiella oxytoca type II secretion system secretin, were purified after cell-free synthesis and their structures analyzed by single particle cryoelectron microscopy. Both homomultimeric, barrel-like structures display a "cup and saucer" architecture. The "saucer" region of both secretins is composed of two distinct rings, with that of PulD being less segmented than that of PscC. Both secretins have a central chamber that is occluded by a plug linked to the chamber walls through hairpin-like structures. Comparisons with published structures from other bacterial systems reveal that secretins have regions of local structural flexibility, probably reflecting their evolved functions in protein secretion and needle assembly.

Research paper thumbnail of A Biologically-Motivated Approach to Image Representation and Its Application to Neuromorphology

Lecture Notes in Computer Science, 2000

Research paper thumbnail of Phasing of the Triatoma virus diffraction data using a cryo-electron microscopy reconstruction

Research paper thumbnail of Cryo-EM structure of the E. coli translating ribosome in complex with SRP and its receptor

Nature Structural & Molecular Biology, 2011

Research paper thumbnail of Three-Dimensional Structure of Canine Adenovirus Serotype 2 Capsid

Journal of Virology, 2008

Research paper thumbnail of SCA: Symmetry-based center assignment of 2D projections of symmetric 3D objects

Journal of Structural Biology, 2007

Research paper thumbnail of The cryo-EM Reconstruction of Drosophila C Virus (DCV) at 5.4 Å

Biophysical Journal, 2013

Research paper thumbnail of Oligomerization paths of the nucleoprotein of influenza A virus

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