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Papers by Paula Veríssimo
Biochimica et biophysica acta, Jan 13, 1996
The cleavage of purified bovine alpha s1-casein separately by cardosin A and cardosin B, two dist... more The cleavage of purified bovine alpha s1-casein separately by cardosin A and cardosin B, two distinct milk-clotting aspartic proteinases (APs) present in the stigmas of the plant Cynara cardunculus L., was studied. Casein digestion peptides were separated either by SDS-PAGE or by reverse-phase HPLC, and their N-terminal amino acid sequences were subsequently determined by automated Edman degradation, thus identifying the cleavage sites. Results showed that both enzymes exert a similar but distinct action on bovine alpha s1-casein. In common they have the preference for the bond Phe23-Phe24, and the cleavage of Trp164-Tyr165 and Phe153-Tyr154. Cardosin A also cleaves the bond Tyr165-Tyr166, whereas Cardosin B cleaves an extra type of bond, Phe150-Arg151, revealing a slightly broader specificity. A model for the action of both enzymes on bovine alpha s1-casein is proposed and discussed. In comparison with the reported action of chymosin on bovine alpha s1-casein, both cardosins proved...
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Advances in experimental medicine and biology, 1998
In the cheese industry, calf chymosin is universally used as one of the main milkclotting enzymes... more In the cheese industry, calf chymosin is universally used as one of the main milkclotting enzymes. However, in traditional cheese manufacturing, several other coagulating agents have also been used for a long time. A special case in Portugal, involves the use of an ...
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Wei sheng wu xue bao = Acta microbiologica Sinica, 2011
In order to provide the basis for application of Streptomyces hygroscopicus BS-112 and its antifu... more In order to provide the basis for application of Streptomyces hygroscopicus BS-112 and its antifungal active substances, we isolated and purified the active substances produced by strain BS-112, and then we examined their chemical structures and determined their inhibitory effects on Aspergillus flavus. We extracted and purified the antifungal active substances from strain BS-112 using column chromatography with X-5 macroporous resin and 200 - 300 mesh silica gel respectively, and then purified them by preparative HPLC with Venusil XBP C18 column. The chemical structures of active components were identified by means of spectroscopic analysis, including mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR). Their minimum inhibitory concentration and minimum fungicidal concentration against Aspergillus flavus were determined by the standard broth microdilution method. We obtained four active components from the fermentation broth of strain BS-112 and their chemical ...
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El Comité de Nomenclatura de la IUBMB ha adoptado el nombre general de fitepsinas (EC 3.4.23.40) ... more El Comité de Nomenclatura de la IUBMB ha adoptado el nombre general de fitepsinas (EC 3.4.23.40) para todas las peptidasas aspárticas (PAs) de origen vegetal (NC-IUBMB, 2006). Sin embargo, en la literatura no es común hallar un nombre universal para referirse a estas peptidasas, sino que los nombres tienden a indicar las especies o los tejidos a partir de los cuales se purifica cada enzima en particular. Las PAs de plantas están distribuidas entre las familias A1, A3, A11 y A12 del clan AA, y la familia A22 del clan AD. La mayoría de las PAs de plantas pertenecen a la familia A1, junto con las enzimas similares a pepsina de diversos orígenes (Rawlings & Barrett, 2004). En esta sección nos centraremos en las PAs presentes en varias especies de la tribu Cardueae Cass. (Cynareae Less.) intensamente estudiadas por el contenido de este tipo de enzimas en sus flores todas ellas integrantes de la familia A1 (Tabla 3.1). La tribu Cardueae es una de las trece tribus en las que ha sido subdiv...
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Journal of integrative bioinformatics, 2011
The strong activity felt in proteomics during the last decade created huge amounts of data, for w... more The strong activity felt in proteomics during the last decade created huge amounts of data, for which the knowledge is limited. Retrieving information from these proteins is the next step. For that, computational techniques are indispensable. Although there is not yet a silver bullet approach to solve the problem of enzyme detection and classification, machine learning formulations such as the state-of-the-art Support Vector Machine (SVM) appear among the most reliable options. A SVM based framework for peptidase analysis, that recognizes the hierarchies demarked in the MEROPS database is presented. Feature selection with SVM-RFE is used to improve the discriminative models and build classifiers computationally more efficient than alignment based techniques.
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Page 1. PEPTILAB: A Computational Tool for Peptidase Data Analysis Lionel Morgado1 Carlos Perei... more Page 1. PEPTILAB: A Computational Tool for Peptidase Data Analysis Lionel Morgado1 Carlos Pereira1, 3 Paula Veríssimo2 António Dourado1 1Center for Informatics and Systems of the University of Coimbra Polo II University ...
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The production of antimycotically active toxins, so-called killer (K) toxins or zymocins, is a wi... more The production of antimycotically active toxins, so-called killer (K) toxins or zymocins, is a widespread phenomenon in various yeast genera, although the most intensively studied killer systems are still those of Saccharomyces cerevisiae and Kluyveromyces lactis (for reviews see 1-2). ...
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Biochimica et biophysica acta, Jan 13, 1996
The cleavage of purified bovine alpha s1-casein separately by cardosin A and cardosin B, two dist... more The cleavage of purified bovine alpha s1-casein separately by cardosin A and cardosin B, two distinct milk-clotting aspartic proteinases (APs) present in the stigmas of the plant Cynara cardunculus L., was studied. Casein digestion peptides were separated either by SDS-PAGE or by reverse-phase HPLC, and their N-terminal amino acid sequences were subsequently determined by automated Edman degradation, thus identifying the cleavage sites. Results showed that both enzymes exert a similar but distinct action on bovine alpha s1-casein. In common they have the preference for the bond Phe23-Phe24, and the cleavage of Trp164-Tyr165 and Phe153-Tyr154. Cardosin A also cleaves the bond Tyr165-Tyr166, whereas Cardosin B cleaves an extra type of bond, Phe150-Arg151, revealing a slightly broader specificity. A model for the action of both enzymes on bovine alpha s1-casein is proposed and discussed. In comparison with the reported action of chymosin on bovine alpha s1-casein, both cardosins proved...
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Advances in experimental medicine and biology, 1998
In the cheese industry, calf chymosin is universally used as one of the main milkclotting enzymes... more In the cheese industry, calf chymosin is universally used as one of the main milkclotting enzymes. However, in traditional cheese manufacturing, several other coagulating agents have also been used for a long time. A special case in Portugal, involves the use of an ...
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Wei sheng wu xue bao = Acta microbiologica Sinica, 2011
In order to provide the basis for application of Streptomyces hygroscopicus BS-112 and its antifu... more In order to provide the basis for application of Streptomyces hygroscopicus BS-112 and its antifungal active substances, we isolated and purified the active substances produced by strain BS-112, and then we examined their chemical structures and determined their inhibitory effects on Aspergillus flavus. We extracted and purified the antifungal active substances from strain BS-112 using column chromatography with X-5 macroporous resin and 200 - 300 mesh silica gel respectively, and then purified them by preparative HPLC with Venusil XBP C18 column. The chemical structures of active components were identified by means of spectroscopic analysis, including mass spectrometry (MS) and nuclear magnetic resonance spectroscopy (NMR). Their minimum inhibitory concentration and minimum fungicidal concentration against Aspergillus flavus were determined by the standard broth microdilution method. We obtained four active components from the fermentation broth of strain BS-112 and their chemical ...
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El Comité de Nomenclatura de la IUBMB ha adoptado el nombre general de fitepsinas (EC 3.4.23.40) ... more El Comité de Nomenclatura de la IUBMB ha adoptado el nombre general de fitepsinas (EC 3.4.23.40) para todas las peptidasas aspárticas (PAs) de origen vegetal (NC-IUBMB, 2006). Sin embargo, en la literatura no es común hallar un nombre universal para referirse a estas peptidasas, sino que los nombres tienden a indicar las especies o los tejidos a partir de los cuales se purifica cada enzima en particular. Las PAs de plantas están distribuidas entre las familias A1, A3, A11 y A12 del clan AA, y la familia A22 del clan AD. La mayoría de las PAs de plantas pertenecen a la familia A1, junto con las enzimas similares a pepsina de diversos orígenes (Rawlings & Barrett, 2004). En esta sección nos centraremos en las PAs presentes en varias especies de la tribu Cardueae Cass. (Cynareae Less.) intensamente estudiadas por el contenido de este tipo de enzimas en sus flores todas ellas integrantes de la familia A1 (Tabla 3.1). La tribu Cardueae es una de las trece tribus en las que ha sido subdiv...
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Journal of integrative bioinformatics, 2011
The strong activity felt in proteomics during the last decade created huge amounts of data, for w... more The strong activity felt in proteomics during the last decade created huge amounts of data, for which the knowledge is limited. Retrieving information from these proteins is the next step. For that, computational techniques are indispensable. Although there is not yet a silver bullet approach to solve the problem of enzyme detection and classification, machine learning formulations such as the state-of-the-art Support Vector Machine (SVM) appear among the most reliable options. A SVM based framework for peptidase analysis, that recognizes the hierarchies demarked in the MEROPS database is presented. Feature selection with SVM-RFE is used to improve the discriminative models and build classifiers computationally more efficient than alignment based techniques.
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Page 1. PEPTILAB: A Computational Tool for Peptidase Data Analysis Lionel Morgado1 Carlos Perei... more Page 1. PEPTILAB: A Computational Tool for Peptidase Data Analysis Lionel Morgado1 Carlos Pereira1, 3 Paula Veríssimo2 António Dourado1 1Center for Informatics and Systems of the University of Coimbra Polo II University ...
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The production of antimycotically active toxins, so-called killer (K) toxins or zymocins, is a wi... more The production of antimycotically active toxins, so-called killer (K) toxins or zymocins, is a widespread phenomenon in various yeast genera, although the most intensively studied killer systems are still those of Saccharomyces cerevisiae and Kluyveromyces lactis (for reviews see 1-2). ...
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