George Seidel | Colorado State University (original) (raw)
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Papers by George Seidel
Journal of Animal Science
The ability to assess fertility of bovine sperm accurately and rapidly would be very useful for r... more The ability to assess fertility of bovine sperm accurately and rapidly would be very useful for research and applications to the cattle industry. Sperm motility and other in vitro tests of sperm normality are only partially correlated with fertility, and lengthy breeding trials are expensive and time consuming. Het- erospermic insemination by mixing sperm from more than one male provides
Journal of Animal Science
The Professional Animal Scientist
The Professional Animal Scientist
The Professional Animal Scientist
The Professional Animal Scientist
The objectives of this study were to de- termine whether calves produced by sexed sperm dif- fere... more The objectives of this study were to de- termine whether calves produced by sexed sperm dif- fered from controls and to what extent the sex ratio of calves was altered by the sexing procedure. Data were collected from 1,169 calves produced from sperm sexed by flow cytometry/cell sorting after staining with Hoechst 33342, and 793 calves produced from control sperm
Reproduction Fertility and Development
Slow-freeze cryopreservation of in vitro-produced bovine embryos often results in poor post-thaw ... more Slow-freeze cryopreservation of in vitro-produced bovine embryos often results in poor post-thaw embryo viability. Results with vitrification tend to be better but do not always produce consistent results for a variety of reasons. Experiments with human and equine embryos have demonstrated improved post-thaw survival when blastocoele fluid is removed before vitrification. We hypothesised that removing blastocoele fluid before vitrification or slow-freeze cryopreservation would improve post-thaw survival of in vitro-produced bovine embryos. Three replicates of embryos were generated from abattoir-derived ovaries using standard techniques. Seven days post-fertilization, embryos were evaluated for quality, and grade 1 or 2 embryos were allocated to 1 of 4 treatments: slow-freeze control (n=103); slow-freeze collapsed (n=106); vitrified control (n=117); vitrified collapsed (n=99). Blastocoele fluid was removed by aspiration with a pipette advanced to the centre of the blastocoele while ...
Animal Reproduction Science
Reproduction, Fertility and Development, 2006
SUMMARY Two groups of yearling beef heifers were fed different levels of nutrition to study the e... more SUMMARY Two groups of yearling beef heifers were fed different levels of nutrition to study the effect of reduced energy intake on fertilization rate and blood levels of progesterone and luteinizing hormone (LH). Control heifers received a ration meeting N.R.C. recommendations for all nutri- ents, while heifers on the restricted diet re- ceived only one-third of the recommended energy. Ova
Journal of Animal Science
The objectives of this study were to determine whether calves produced by sexed sperm differed fr... more The objectives of this study were to determine whether calves produced by sexed sperm differed from controls and to what extent the sex ratio of calves was altered by the sexing procedure. Data were collected from 1,169 calves produced from sperm sexed by flow cytometry/cell sorting after staining with Hoechst 33342, and 793 calves produced from control sperm during breeding trials between 1997 and 2001. Least squares ANOVA were completed using factors of treatment (sexed vs. control sperm), 19 management groups from 13 field trials, and calf sex. Responses analyzed include gestation length, birth weight, calving ease, calf vigor, weaning weight, abortion rate, and death rates (neonatal and through weaning). No significant difference was observed for any response due to treatment or treatment interactions (P > 0.10). Therefore, calves produced from sexed sperm grew and developed normally both pre- and postnatally. A neurological disorder was observed in four control calves and on...
Reproduction, Fertility and Development, 2009
Journal of Animal Science
The ability to assess fertility of bovine sperm accurately and rapidly would be very useful for r... more The ability to assess fertility of bovine sperm accurately and rapidly would be very useful for research and applications to the cattle industry. Sperm motility and other in vitro tests of sperm normality are only partially correlated with fertility, and lengthy breeding trials are expensive and time consuming. Het- erospermic insemination by mixing sperm from more than one male provides
Journal of Animal Science
The Professional Animal Scientist
The Professional Animal Scientist
The Professional Animal Scientist
The Professional Animal Scientist
The objectives of this study were to de- termine whether calves produced by sexed sperm dif- fere... more The objectives of this study were to de- termine whether calves produced by sexed sperm dif- fered from controls and to what extent the sex ratio of calves was altered by the sexing procedure. Data were collected from 1,169 calves produced from sperm sexed by flow cytometry/cell sorting after staining with Hoechst 33342, and 793 calves produced from control sperm
Reproduction Fertility and Development
Slow-freeze cryopreservation of in vitro-produced bovine embryos often results in poor post-thaw ... more Slow-freeze cryopreservation of in vitro-produced bovine embryos often results in poor post-thaw embryo viability. Results with vitrification tend to be better but do not always produce consistent results for a variety of reasons. Experiments with human and equine embryos have demonstrated improved post-thaw survival when blastocoele fluid is removed before vitrification. We hypothesised that removing blastocoele fluid before vitrification or slow-freeze cryopreservation would improve post-thaw survival of in vitro-produced bovine embryos. Three replicates of embryos were generated from abattoir-derived ovaries using standard techniques. Seven days post-fertilization, embryos were evaluated for quality, and grade 1 or 2 embryos were allocated to 1 of 4 treatments: slow-freeze control (n=103); slow-freeze collapsed (n=106); vitrified control (n=117); vitrified collapsed (n=99). Blastocoele fluid was removed by aspiration with a pipette advanced to the centre of the blastocoele while ...
Animal Reproduction Science
Reproduction, Fertility and Development, 2006
SUMMARY Two groups of yearling beef heifers were fed different levels of nutrition to study the e... more SUMMARY Two groups of yearling beef heifers were fed different levels of nutrition to study the effect of reduced energy intake on fertilization rate and blood levels of progesterone and luteinizing hormone (LH). Control heifers received a ration meeting N.R.C. recommendations for all nutri- ents, while heifers on the restricted diet re- ceived only one-third of the recommended energy. Ova
Journal of Animal Science
The objectives of this study were to determine whether calves produced by sexed sperm differed fr... more The objectives of this study were to determine whether calves produced by sexed sperm differed from controls and to what extent the sex ratio of calves was altered by the sexing procedure. Data were collected from 1,169 calves produced from sperm sexed by flow cytometry/cell sorting after staining with Hoechst 33342, and 793 calves produced from control sperm during breeding trials between 1997 and 2001. Least squares ANOVA were completed using factors of treatment (sexed vs. control sperm), 19 management groups from 13 field trials, and calf sex. Responses analyzed include gestation length, birth weight, calving ease, calf vigor, weaning weight, abortion rate, and death rates (neonatal and through weaning). No significant difference was observed for any response due to treatment or treatment interactions (P > 0.10). Therefore, calves produced from sexed sperm grew and developed normally both pre- and postnatally. A neurological disorder was observed in four control calves and on...
Reproduction, Fertility and Development, 2009