John Loike | Columbia University (original) (raw)

Papers by John Loike

Research paper thumbnail of Lactate transport in macrophages

Journal of Immunology, Mar 1, 1993

Glycolytic regulation of neuroin ammatory response: Role of monocarboxylate transporter 4

Research paper thumbnail of Phagocytosis

Research paper thumbnail of Mitochondrial Replacement Therapy, Jewish and Catholic Ethical Perspectives

Mitochondrial Replacement Therapy is an ethically controversial procedure that allows women with ... more Mitochondrial Replacement Therapy is an ethically controversial procedure that allows women with genetically transmitted mutations in their mitochondria to have healthy children. Using this procedure, healthy mitochondria from a third party can be donated in the fertilization process to create a healthy child from three genetic “parents.” There is much concern about the safety and ethics of this procedure and presenting the Catholic and Jewish perspectives of Mitochondrial Replacement Therapy can offer insights into the morally complexity of the ethical issues surrounding this innovative medical procedure.

Research paper thumbnail of Human gene therapy - The road to today and beyond

Journal of Molecular and Genetic Medicine, 2017

Research paper thumbnail of Isolation, purification and initial characterisation

Research paper thumbnail of Ethical challenges in tampering with the Holy Grail: Gene editing technologies

Journal of Molecular and Genetic Medicine, Jul 25, 2017

Research paper thumbnail of Biotechnological Applications to Produce Animal-free Meat and Milk: Ethical Considerations

Food & nutrition journal, Jun 14, 2018

Research paper thumbnail of Will CRISPR Germline Engineering Close the Door to an Open Future?

Science and Engineering Ethics, Oct 24, 2018

The bioethical principle of autonomy is problematic regarding the future of the embryo who lacks ... more The bioethical principle of autonomy is problematic regarding the future of the embryo who lacks the ability to self-advocate but will develop this defining human capacity in time. Recent experiments explore the use of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 for germline engineering in the embryo, which alters future generations. The embryo's inability to express an autonomous decision is an obvious bioethical challenge of germline engineering. The philosopher Joel Feinberg acknowledged that autonomy is developing in children. He advocated that to reserve this future autonomy, parents should be guided to make ethical decisions that provide children with open futures. Here, Feinberg's 1980 open future theory is extended to the human embryo in the context of CRISPR germline engineering. Although the embryo does not possess the autonomous decisionmaking capacity at the time of germline engineering, the parental decision to permanently change the unique genetic fabric of the embryo and subsequent generations disregards future autonomy. Therefore, germline engineering in many instances is objectionable considering Feinberg's open future theory.

Research paper thumbnail of A comparison of the mechanisms of action of VP-16-213 and podophyllotoxin

PubMed, 1977

A comparison of the mechanism of action of VP 16-213 and podophyllotoxin has revealed that althou... more A comparison of the mechanism of action of VP 16-213 and podophyllotoxin has revealed that although both drugs inhibit nucleoside uptake in HeLa cells, they exhibit other biological properties which are quite distinct. Podophyllotoxin inhibits microtubule assembly in vitro while VP-16-213 has no effect in this system. VP-16-213 induces single-stranded breaks in DNA in HeLa cells, an effect which may explain the cytoxicity of VP-16-213. In contrast to VP-16-213-treated cells, cells treated with podophyllotoxin maintain DNA integrity and show no evidence of single-stranded breaks.

Research paper thumbnail of Bioethical challenges of alternative medicine

Alternative & Integrative Medicine, Dec 19, 2013

Research paper thumbnail of The Jewish Perspective in Creating Human Embryos Using Cloning Technologies

Research paper thumbnail of Etoposide and Teniposide

Cambridge University Press eBooks, Nov 30, 1990

Research paper thumbnail of Determination of structure

Cambridge University Press eBooks, Nov 30, 1990

Research paper thumbnail of The expression of creatine transporters is regulated by creatine and differentiation

Journal of Molecular and Cellular Cardiology, 1987

Injection of isoproterenol markedly increased the level of c-fos mRNA in the hearts of mice, rats... more Injection of isoproterenol markedly increased the level of c-fos mRNA in the hearts of mice, rats, and Syrian hamsters. The calcium channel blockers verapamil, nifedipine, and diltiazem had no effect on c-fos induction. In rat heart, c-fos expression was also stimulated by the alpha-adrenergic agonist phenylephrine, histamine, and prostaglandin E 1. The Hl-receptor antagonist pyrilamine, but not the H2-receptor antagonists ranitidine and cimetidine, blocked the histamine-induced expression ofc-fos. We conclude that in the heart, hormones which increase cAMP and cytosolic Ca 2+, such as beta-adrenergic agonists and prostaglandin El, and/or stimulate the turnover of phosphatidylinositides, such as alpha-adrenergic agonists and histamine Hl-receptor agonists, regulate c-fos gene expression. It is suggested that fos protein plays a role in the mechanisms of neurotransmitters and hormones that modulate the functioning of the heart and possibly in cardiac hypertrophy, degeneration, and necrosis.

Research paper thumbnail of A registry of the natural lignans

Cambridge University Press eBooks, Nov 30, 1990

Research paper thumbnail of Activated platelets form protected zones of adhesion on fibrinogen and fibronectin-coated surfaces

Journal of Cell Biology, May 15, 1993

Research paper thumbnail of Lignans

Lignans are widely occurring plant compounds and are closely related to lignin, which forms the w... more Lignans are widely occurring plant compounds and are closely related to lignin, which forms the woody component of trees and other plants. The lignans are characterized by their dimeric composition from cinnamic acids, and they are attracting increasing attention as a result of their pharmacological properties. The volume surveys the chemical, biological and clinical properties of lignans as well as providing information on their isolation, purification, identification and chemical synthesis. The volume also explores fully the potential use of these compounds as antiviral and antitumour agents, and thus provides a wide-ranging survey of their pharmacology and chemistry. The text is fully documented and referenced and provides the only up-to-date compilation on this subject. The volume is suitable for research scientists in the fields of organic chemistry, biochemistry, oncology, pharmacology, toxicology and botany.

Research paper thumbnail of Increased ATP and creatine phosphate turnover in phagocytosing mouse peritoneal macrophages

Journal of Biological Chemistry, Oct 1, 1979

Research paper thumbnail of To students and colleagues in Westfield College, 1965–1984

Cambridge University Press eBooks, Nov 30, 1990

Research paper thumbnail of Lignans; chemical, biological and clinical properties

Comparative Biochemistry and Physiology Part A: Physiology, 1991

Research paper thumbnail of Lactate transport in macrophages

Journal of Immunology, Mar 1, 1993

Glycolytic regulation of neuroin ammatory response: Role of monocarboxylate transporter 4

Research paper thumbnail of Phagocytosis

Research paper thumbnail of Mitochondrial Replacement Therapy, Jewish and Catholic Ethical Perspectives

Mitochondrial Replacement Therapy is an ethically controversial procedure that allows women with ... more Mitochondrial Replacement Therapy is an ethically controversial procedure that allows women with genetically transmitted mutations in their mitochondria to have healthy children. Using this procedure, healthy mitochondria from a third party can be donated in the fertilization process to create a healthy child from three genetic “parents.” There is much concern about the safety and ethics of this procedure and presenting the Catholic and Jewish perspectives of Mitochondrial Replacement Therapy can offer insights into the morally complexity of the ethical issues surrounding this innovative medical procedure.

Research paper thumbnail of Human gene therapy - The road to today and beyond

Journal of Molecular and Genetic Medicine, 2017

Research paper thumbnail of Isolation, purification and initial characterisation

Research paper thumbnail of Ethical challenges in tampering with the Holy Grail: Gene editing technologies

Journal of Molecular and Genetic Medicine, Jul 25, 2017

Research paper thumbnail of Biotechnological Applications to Produce Animal-free Meat and Milk: Ethical Considerations

Food & nutrition journal, Jun 14, 2018

Research paper thumbnail of Will CRISPR Germline Engineering Close the Door to an Open Future?

Science and Engineering Ethics, Oct 24, 2018

The bioethical principle of autonomy is problematic regarding the future of the embryo who lacks ... more The bioethical principle of autonomy is problematic regarding the future of the embryo who lacks the ability to self-advocate but will develop this defining human capacity in time. Recent experiments explore the use of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 for germline engineering in the embryo, which alters future generations. The embryo's inability to express an autonomous decision is an obvious bioethical challenge of germline engineering. The philosopher Joel Feinberg acknowledged that autonomy is developing in children. He advocated that to reserve this future autonomy, parents should be guided to make ethical decisions that provide children with open futures. Here, Feinberg's 1980 open future theory is extended to the human embryo in the context of CRISPR germline engineering. Although the embryo does not possess the autonomous decisionmaking capacity at the time of germline engineering, the parental decision to permanently change the unique genetic fabric of the embryo and subsequent generations disregards future autonomy. Therefore, germline engineering in many instances is objectionable considering Feinberg's open future theory.

Research paper thumbnail of A comparison of the mechanisms of action of VP-16-213 and podophyllotoxin

PubMed, 1977

A comparison of the mechanism of action of VP 16-213 and podophyllotoxin has revealed that althou... more A comparison of the mechanism of action of VP 16-213 and podophyllotoxin has revealed that although both drugs inhibit nucleoside uptake in HeLa cells, they exhibit other biological properties which are quite distinct. Podophyllotoxin inhibits microtubule assembly in vitro while VP-16-213 has no effect in this system. VP-16-213 induces single-stranded breaks in DNA in HeLa cells, an effect which may explain the cytoxicity of VP-16-213. In contrast to VP-16-213-treated cells, cells treated with podophyllotoxin maintain DNA integrity and show no evidence of single-stranded breaks.

Research paper thumbnail of Bioethical challenges of alternative medicine

Alternative & Integrative Medicine, Dec 19, 2013

Research paper thumbnail of The Jewish Perspective in Creating Human Embryos Using Cloning Technologies

Research paper thumbnail of Etoposide and Teniposide

Cambridge University Press eBooks, Nov 30, 1990

Research paper thumbnail of Determination of structure

Cambridge University Press eBooks, Nov 30, 1990

Research paper thumbnail of The expression of creatine transporters is regulated by creatine and differentiation

Journal of Molecular and Cellular Cardiology, 1987

Injection of isoproterenol markedly increased the level of c-fos mRNA in the hearts of mice, rats... more Injection of isoproterenol markedly increased the level of c-fos mRNA in the hearts of mice, rats, and Syrian hamsters. The calcium channel blockers verapamil, nifedipine, and diltiazem had no effect on c-fos induction. In rat heart, c-fos expression was also stimulated by the alpha-adrenergic agonist phenylephrine, histamine, and prostaglandin E 1. The Hl-receptor antagonist pyrilamine, but not the H2-receptor antagonists ranitidine and cimetidine, blocked the histamine-induced expression ofc-fos. We conclude that in the heart, hormones which increase cAMP and cytosolic Ca 2+, such as beta-adrenergic agonists and prostaglandin El, and/or stimulate the turnover of phosphatidylinositides, such as alpha-adrenergic agonists and histamine Hl-receptor agonists, regulate c-fos gene expression. It is suggested that fos protein plays a role in the mechanisms of neurotransmitters and hormones that modulate the functioning of the heart and possibly in cardiac hypertrophy, degeneration, and necrosis.

Research paper thumbnail of A registry of the natural lignans

Cambridge University Press eBooks, Nov 30, 1990

Research paper thumbnail of Activated platelets form protected zones of adhesion on fibrinogen and fibronectin-coated surfaces

Journal of Cell Biology, May 15, 1993

Research paper thumbnail of Lignans

Lignans are widely occurring plant compounds and are closely related to lignin, which forms the w... more Lignans are widely occurring plant compounds and are closely related to lignin, which forms the woody component of trees and other plants. The lignans are characterized by their dimeric composition from cinnamic acids, and they are attracting increasing attention as a result of their pharmacological properties. The volume surveys the chemical, biological and clinical properties of lignans as well as providing information on their isolation, purification, identification and chemical synthesis. The volume also explores fully the potential use of these compounds as antiviral and antitumour agents, and thus provides a wide-ranging survey of their pharmacology and chemistry. The text is fully documented and referenced and provides the only up-to-date compilation on this subject. The volume is suitable for research scientists in the fields of organic chemistry, biochemistry, oncology, pharmacology, toxicology and botany.

Research paper thumbnail of Increased ATP and creatine phosphate turnover in phagocytosing mouse peritoneal macrophages

Journal of Biological Chemistry, Oct 1, 1979

Research paper thumbnail of To students and colleagues in Westfield College, 1965–1984

Cambridge University Press eBooks, Nov 30, 1990

Research paper thumbnail of Lignans; chemical, biological and clinical properties

Comparative Biochemistry and Physiology Part A: Physiology, 1991

Research paper thumbnail of The Future of Reproductive Medicine: What Does Halachah Say?

Research paper thumbnail of Does Halakhah consider female infertility an illness

Hakirah, 2018

Infertility is both a global problem and a particular problem for the Jewish community. According... more Infertility is both a global problem and a particular problem for the Jewish community. According to the latest assessment by the Center for Disease Control, the rate of women between the ages of 15 and 44 who suffer from some form of impaired fecundity (a physical inability to have a child) is almost 12% (approximately 7.5 million). 1,2 In the Jewish community, estimates of infertility are as high as one in six women. 3 To reduce infertility, biotechnologies and medical innovations have been, and are currently being, developed to allow men and women to have children in ways that have never been previously considered possible. In fact, the last forty years have been transformational in reproduction medicine by providing a wide variety of new drugs, procedures (such as artificial insemination, in vitro fertilization, hormonal treatments) and surgical interventions to treat infertility in women. 4 Current interventions include 1