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Scientific Reports
Maize protein quality is determined by the composition of its endosperm proteins, which are class... more Maize protein quality is determined by the composition of its endosperm proteins, which are classified as nutritionally poor zeins (prolamin and prolamin-like) and nutritionally rich non-zeins (albumin, globulin, glutelin-like, and glutelin). Protein quality is considerably higher in opaque-2 mutants due to increased content of non-zeins over zeins. However, the opaque-2 endosperm is soft, which leads to poor agronomic performance and post-harvest infestation. Endosperm modification of opaque-2 had led to the development of Quality Protein Maize (QPM), which has higher protein quality along with hard kernel endosperm. The present study was planned to analyze the expression dynamics of different protein fractions in the endospem of developing maize kernel in normal, opaque-2 and QPM in response to the introgression of endosperm modifiers. Results revealed that albumin and globulin content decreases, whereas, prolamin, prolamin-like, glutelin-like, and glutelin content increases with ...
International Journal of Pharma Research and Health Sciences
Infectious diseases greatly increase death rate in world population that dysfunction or breakdown... more Infectious diseases greatly increase death rate in world population that dysfunction or breakdown social life, effect on economy. Nature powers are erratic. Therefore it is truer that some infectious diseases come without giving any warning by nature. The diseases which spread one person to other person in particular area, city very rapidly is known as Epidemics and the diseases which spread from one country to others known as Pandemic. Choleric plague, smallpox, AIDS and influenza etc. was very dangerous communicable diseases in past years ago and some are also present now like AIDS and COVID, it is the most devastating example of pandemics. Disease outbreaks are occurring when disease spread unexpectedly and rapidly. Epidemics and Pandemics damage to humans in few time.
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 2015
India is the largest producer of buffalo milk in the world. Buffalo milk is rich in minerals like... more India is the largest producer of buffalo milk in the world. Buffalo milk is rich in minerals like calcium and magnesium, containing 100 % more fat and less cholesterol than cow milk. Physio-chemical properties of dairy products depend upon the size and distribution of milk fat globules (MFG). Individual MFG from Murrah buffalo were analysed as a function of size and distribution and comparisons were made with MFG of crossbred (HF X Sahiwal) cows. Crescents associated with MFG could serve as a source of RNA of mammary epithelial cells and that would be a non-invasive method of epithelial cell RNA. Acridine orange staining of fresh milk was visualized by light microscopy under red and green channel filters. MFG and MFG with crescents were analysed using imageJ image analysis software. The percentage of large sized MFG (area [20 lm 2) was greater and the percentage of small sized MFG (area \10 lm 2) was lower in buffalo milk than that of cow. The percentage of crescents was \1 % of total MFG in both the species. These results indicate that MFG in buffalo milk is larger than MFG of cow milk. RNA isolation from crescents of buffalo MFG and its suitability for microarray analysis remains to be determined.
Scientific Reports
Maize protein quality is determined by the composition of its endosperm proteins, which are class... more Maize protein quality is determined by the composition of its endosperm proteins, which are classified as nutritionally poor zeins (prolamin and prolamin-like) and nutritionally rich non-zeins (albumin, globulin, glutelin-like, and glutelin). Protein quality is considerably higher in opaque-2 mutants due to increased content of non-zeins over zeins. However, the opaque-2 endosperm is soft, which leads to poor agronomic performance and post-harvest infestation. Endosperm modification of opaque-2 had led to the development of Quality Protein Maize (QPM), which has higher protein quality along with hard kernel endosperm. The present study was planned to analyze the expression dynamics of different protein fractions in the endospem of developing maize kernel in normal, opaque-2 and QPM in response to the introgression of endosperm modifiers. Results revealed that albumin and globulin content decreases, whereas, prolamin, prolamin-like, glutelin-like, and glutelin content increases with ...
International Journal of Pharma Research and Health Sciences
Infectious diseases greatly increase death rate in world population that dysfunction or breakdown... more Infectious diseases greatly increase death rate in world population that dysfunction or breakdown social life, effect on economy. Nature powers are erratic. Therefore it is truer that some infectious diseases come without giving any warning by nature. The diseases which spread one person to other person in particular area, city very rapidly is known as Epidemics and the diseases which spread from one country to others known as Pandemic. Choleric plague, smallpox, AIDS and influenza etc. was very dangerous communicable diseases in past years ago and some are also present now like AIDS and COVID, it is the most devastating example of pandemics. Disease outbreaks are occurring when disease spread unexpectedly and rapidly. Epidemics and Pandemics damage to humans in few time.
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 2015
India is the largest producer of buffalo milk in the world. Buffalo milk is rich in minerals like... more India is the largest producer of buffalo milk in the world. Buffalo milk is rich in minerals like calcium and magnesium, containing 100 % more fat and less cholesterol than cow milk. Physio-chemical properties of dairy products depend upon the size and distribution of milk fat globules (MFG). Individual MFG from Murrah buffalo were analysed as a function of size and distribution and comparisons were made with MFG of crossbred (HF X Sahiwal) cows. Crescents associated with MFG could serve as a source of RNA of mammary epithelial cells and that would be a non-invasive method of epithelial cell RNA. Acridine orange staining of fresh milk was visualized by light microscopy under red and green channel filters. MFG and MFG with crescents were analysed using imageJ image analysis software. The percentage of large sized MFG (area [20 lm 2) was greater and the percentage of small sized MFG (area \10 lm 2) was lower in buffalo milk than that of cow. The percentage of crescents was \1 % of total MFG in both the species. These results indicate that MFG in buffalo milk is larger than MFG of cow milk. RNA isolation from crescents of buffalo MFG and its suitability for microarray analysis remains to be determined.