Maria Olivera | Universidad Nacional de Córdoba (original) (raw)
Papers by Maria Olivera
Livestock Science, 2012
The present study investigated the effect of three different dietary fats [saturated fat (SAT) (3... more The present study investigated the effect of three different dietary fats [saturated fat (SAT) (3.1% TMR DM); linseed oil (LINA) (2.7% TMR DM) or sunflower oil (SUNA) (2.7% TMR DM) added with docosahexaenoic acid rich algae (0.4% TMR DM)] on SREBP-1 protein expression in mammary gland tissue of German Holstein cows (n= 18) in the context of milk yield, milk fat content and milk fatty acid composition. Prerequisite for this investigation was the development of a Western blot methodology adapted for bovine mammary gland tissue using two different primary antibodies to specifically detect and differentiate between precursor (pSREBP-1, 125 kDa) and transcriptionally active SREBP-1 (mSREBP-1, 68 kDa). Upon a ten-week feeding intervention, significantly reduced milk fat contents, total saturated fatty acid amounts and single saturated fatty acid concentrations (C10:0, C12:0, C14:0, C16:0) were obtained in LINA and SUNA group, whereas milk yields did not substantially differ between feeding groups. The assumption that the effects of plant oil/DHA rich algae supplementation on milk fat composition were mediated by shifted SREBP-1 protein expression were only confirmed in regard to slightly reduced protein expression levels of pSREBP-1 in SUNA group tissue samples (P = 0.087), whereas similar protein expression levels of transcriptionally active mSREBP-1 were found in SAT, LINA and SUNA group.
Background: In the hospital setting is frequent to manipulate solid dosage forms (SDF, tablets 16... more Background: In the hospital setting is frequent to manipulate solid dosage forms (SDF, tablets 16and capsules), which can affect their physicochemical and biopharmaceutical properties, the pharmacoloeffect and sometimes to cause the appearance of undesirable side effects. Objectives: To identify the medicines whose SDF is altered in a Hospital of Cordoba (Argentina), to determine how these modifications are made and to establish whether they were properly performed or not. Methods: We retrospectively analyzed the prescriptions made between the 5th and the 11th of March of 2012 and identified impaired requesting SDF modifications. Open interviews were held with nurses and service visits to each hospital to determine how they manipulated the SDF. Results: We analyzed 700 prescriptions for 113 patients, of which 61 (54%) had manipulations of the received SDF (49 for medical prescription, 7 because they had nasogastric tubes, 3 due to patient choice and 2 by nursing decision). Twenty th...
![Research paper thumbnail of [Modification of solid dosage forms: survey in a hospital of the Province of Cordoba]](https://attachments.academia-assets.com/103059336/thumbnails/1.jpg)
](Revista de la Facultad de Ciencias Médicas (Córdoba, Argentina), 2013
In the hospital setting is frequent to manipulate solid dosage forms (SDF, tablets 16 and capsule... more In the hospital setting is frequent to manipulate solid dosage forms (SDF, tablets 16 and capsules), which can affect their physicochemical and biopharmaceutical properties, the pharmacoloeffect and sometimes to cause the appearance of undesirable side effects. To identify the medicines whose SDF is altered in a Hospital of Cordoba (Argentina), to determine how these modifications are made and to establish whether they were properly performed or not. We retrospectively analyzed the prescriptions made between the 5th and the 11th of March of 2012 and identified impaired requesting SDF modifications. Open interviews were held with nurses and service visits to each hospital to determine how they manipulated the SDF. We analyzed 700 prescriptions for 113 patients, of which 61 (54%) had manipulations of the received SDF (49 for medical prescription, 7 because they had nasogastric tubes, 3 due to patient choice and 2 by nursing decision). Twenty three medicines were manipulated, but only ...
Scientific Reports
The development of new treatments capable of controlling infections and pain related to burns con... more The development of new treatments capable of controlling infections and pain related to burns continues to be a challenge. Antimicrobials are necessary tools, but these can be cytotoxic for regenerating cells. In this study, antibiotic-anesthetic (AA) smart systems obtained by ionic complexation of polyelectrolytes with ciprofloxacin and lidocaine were obtained as films and hydrogels. Ionic complexation with sodium alginate and hyaluronate decreased cytotoxicity of ciprofloxacin above 70% in a primary culture of isolated fibroblasts (p < 0.05). In addition, the relative levels of the proteins involved in cell migration, integrin β1 and p-FAK, increased above 1.5 times (p < 0.05) with no significant differences in cell mobility. Evaluation of the systems in a deep second-degree burn model revealed that reepithelization rate was AA-films = AA-hydrogels > control films > no treated > reference cream (silver sulfadiazine cream). In addition, appendage conservation and com...
Purpose: To evaluate the stability of an extemporaneously prepared 7% chloral hydrate syrup under... more Purpose: To evaluate the stability of an extemporaneously prepared 7% chloral hydrate syrup under different conditions of storage and dispensing. Methods: Three batches of 7% chloral hydrate syrup were prepared. Each batch was stored in 50 light-resistant glass containers of 60 mL with child-resistant caps and in two bottles of 1000 mL to simulate two forms of dispensing, mono and multi-dose, respectively. Twenty five mono-dose bottles and a multi-dose bottle of each batch were stored under room conditions (20 ± 1°C) and the rest of the samples were stored in the fridge (5 ± 2°C). The physical, chemical and microbiological stability was evaluated for 180 days. Stability was defined as retention of at least 95% of the initial concentration of chloral hydrate, the absence of both visible particulate matter, or color and/or odor changes and the compliance with microbiological attributes of non-sterile pharmaceutical products. Results: At least 98% of the initial chloral hydrate concent...
Fil: Olivera, Maria Eugenia. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Arge... more Fil: Olivera, Maria Eugenia. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina.
Pharmacy Education, 2018
The need to know the current state of professional practice in Argentina in different areas, and ... more The need to know the current state of professional practice in Argentina in different areas, and the absence of systematic information about it, led to the organisation of a workshop with pharmacists from several different practice settings. The workshop provided a space for reflection and dialogue for the participants committed to improving pharmacy education and professional practice in Argentina. The objective of this report was to document the main points from this workshop; which allowed the participants to reinforce their identity as pharmacists, review health policies in Argentina and identify the strengths and weaknesses of pharmacy education and professional practice in order to work on the gaps observed. A list of future actions to be implemented in the work setting was drawn up and the representatives who would allow the implementation of necessary action plans and who expressed their commitment to act upon them were identified. This experience highlighted the need to str...
Diabetes Mellitus is a chronic disease related to hyperglycaemia, and its treatment purposes are ... more Diabetes Mellitus is a chronic disease related to hyperglycaemia, and its treatment purposes are preventing and reducing the complications which cause more than 70% of deaths. Health programs with drug supply at public health facilities in Alta Gracia City were analized: Hospital Arturo Umberto Illia (HAUI), depending on the Health Ministry of the Province of Cordoba, and 12 dispensaries, depending on the Direccion de Salud Publica (DSP) of the Municipality of Alta Gracia. A total of 540 patients were identified in the merged data base and 49 were found in both settings. Drug consumptions, expressed in DDD per 100 patient under program by day, were: 25.2 in HAUI and 24.6 in DSP for Glibenclamide; 17.6 in HAUI and 13.1 in DSP for Metformin; 59.0 in HAUI for Insulin. The information obtained from the Pharmacy Services let the development of a diabetic patient data base and a consumption drug utilization study.
Objetivos: Describir las caracteristicas de la poblacion de pacientes diabeticos atendida en el s... more Objetivos: Describir las caracteristicas de la poblacion de pacientes diabeticos atendida en el subsector publico de salud de la ciudad de Alta Gracia. Analizar el uso de la cantidad de dispensaciones mensuales como indicador de adherencia al tratamiento, comparando resultados entre establecimientos sanitarios. Valorar la coordinacion de efectores publicos en la atencion de pacientes. Materiales y metodos: Diseno: estudio observacional, descriptivo-analitico. Lugar: Hospital Arturo Umberto Illia (HAUI) y Direccion de Salud Publica (DSP). Alta Gracia (Provincia de Cordoba, Argentina). Poblacion: Pacientes diabeticos pertenecientes a programas de salud de efectores publicos. Mediciones principales: Variables demograficas y epidemiologicas, cantidad de dispensaciones mensuales, adherencia global porcentual y coordinacion de efectores. Resultados: Del total de pacientes diabeticos (n=540): 52% se atendian en el HAUI, 39% en la DSP y 9% en ambos centros; 55% eran mujeres y la edad promed...
Ciências da Saúde: desafios, perspectivas e possibilidades - Volume 1, 2021
Cutaneous leishmaniasis (CL) is a neglected tropical disease endemic in ~90 countries, with an in... more Cutaneous leishmaniasis (CL) is a neglected tropical disease endemic in ~90 countries, with an increasing incidence. Presently available pharmacotherapy implies the systemic administration of moderately/very toxic drugs. Miltefosine (Milt) is the only FDA approved drug to treat CL via the oral route (Impavido®). It produces side effects; in particular, teratogenic effects are of concern. A topical treatment would have the great advantage of minimising the systemic circulation of the drug, preventing side effects. We prepared dispersions containing Milt and liposomes to modulate trans-epidermal penetration and evaluated in vivo efficacy. Treatments were topically administered to BALB/c mice infected with Leishmania (Leishmania) amazonensis. The dispersions containing 0.5 % Milt eliminated 99 % of the parasites and cured the lesions with a complete re-epithelisation, no visible scar and re-growth of hair. Fluid liposomes decreased the time to heal the lesion and the time needed to eliminate viable amastigotes from the lesion site. Relapse of the infection was not found one month after treatment in any case. A topical Milt formulation including fluid liposomes seems a promising treatment against CL.
Revista de la Facultad de Ciencias Medicas (Cordoba, Argentina), Dec 26, 2018
Plant extracts can be obtained to carry bioactive compounds, useful for prevention and treatment ... more Plant extracts can be obtained to carry bioactive compounds, useful for prevention and treatment of different illnesses. This also supports the intake of teas as functional beverages. Nonetheless, it is incompletely known whether these extracts can act as effective sources and vehicles de phenolic compounds (phenolics/polyphenols) to reach their targets. To establish whether phytoextract ingestion modified in a sex-dependent manner the phenolic bioavailability and redox response in liver and kidney. BALB/C mice ingested for a month 100 mg/Kg/d of extracts (tea-like) from Aspidosperma quebracho-blanco (AQB), Lantana grisebachii (LG) or Ilex paraguariensis (IP). Then, phenolics, peroxides and nitrites were analyzed by spectrophotometry. Also, phenolic permeation from digested and undigested extracts was evaluated in vitro with a rat jejunum-based assay. Phenolic permeation depended on extract digestion. In males, IP showed a special time course of hepatic phenolics, whereas all extrac...
European Journal of Pharmaceutical Sciences, 2017
One of the main obstacles to the successful treatment of tuberculosis is the poor and variable or... more One of the main obstacles to the successful treatment of tuberculosis is the poor and variable oral bioavailability of rifampicin (RIF), which is mainly due to its low hydrophilicity and dissolution rate. The aim of this work was to obtain a hydrophilic new material that allows a very fast dissolution rate of RIF and therefore is potentially useful in the development of oral solid dosage forms. The acid form of carboxymethylcellulose (CMC) was co-processed with RIF by solvent impregnation to obtain CMC-RIF powder, which was characterized by polarized optical microscopy, powder x-ray diffraction, DSC-TGA, hot stage microscopy, 13 C and 15 N solid-state NMR and FT-IR spectroscopy. In addition, the CMC-RIF matrices were subjected to water uptake and dissolution studies to assess hydrophilicity and release kinetics. CMC-RIF is a crystalline solid dispersion. Solid-state characterization indicated that no ionic interaction occurred between the components, but RIF crystallized as a zwitterion over the surface of CMC, which drastically increased the hydrophilicity of the solid. The CMC-RIF matrices significantly improved the water uptake of RIF and disintegrated in a very short period immediately releasing RIF. As CMC improves the hydrophilicity and delivery properties of RIF, CMC-RIF is very useful in the design of oral solid dosage forms with very fast dissolution of RIF, either alone or in combination with other antitubercular drugs.
Results in pharma sciences, 2016
The United States Pharmacopeia (USP) General Chapters Dissolution 〈711〉 and Disintegration and Di... more The United States Pharmacopeia (USP) General Chapters Dissolution 〈711〉 and Disintegration and Dissolution of Dietary Supplements 〈2040〉 allows the use of enzymes in dissolution media when gelatin capsules do not conform to dissolution specifications due to cross linking. Possible interactions between enzymes and surfactants when used together in dissolution media could result in loss of the enzymatic activity. Pepsin is an enzyme commonly used in dissolution media, and in this work, the activity of pepsin was determined in the presence of different surfactants as usually found in case of dissolution tests of certain gelatin capsule formulations. Pepsin enzymatic activity was determined according to the Ninth Edition of the Food Chemicals Codex (FCC) 9 method, in dissolution conditions: simulated gastric fluid, 37 °C and 50 rpm. Sodium dodecyl sulfate (SDS), cetyltrimethyl ammonium bromide (CTAB), polysorbate 80 (Tween 80) and octoxynol 9 (Triton X100) in concentrations above and be...
Farmacia hospitalaria : órgano oficial de expresión científica de la Sociedad Española de Farmacia Hospitalaria
To evaluate the stability of an extemporaneously prepared 7% chloral hydrate syrup under differen... more To evaluate the stability of an extemporaneously prepared 7% chloral hydrate syrup under different conditions of storage and dispensing. Three batches of 7% chloral hydrate syrup were prepared. Each batch was stored in 50 light-resistant glass containers of 60 mL with child-resistant caps and in two bottles of 1000 mL to simulate two forms of dispensing, mono and multi-dose, respectively. Twenty five mono-dose bottles and a multi-dose bottle of each batch were stored under room conditions (20 ± 1 °C) and the rest of the samples were stored in the fridge (5 ± 2 °C). The physical, chemical and microbiological stability was evaluated for 180 days. Stability was defined as retention of at least 95% of the initial concentration of chloral hydrate, the absence of both visible particulate matter, or color and/or odor changes and the compliance with microbiological attributes of non-sterile pharmaceutical products. At least 98% of the initial chloral hydrate concentration remained throughou...
International Journal of Pharmaceutics, 2010
Thiourea (Tu) crystallizes with barbituric acid (H 2 ba) and 2-thiobarbituric (H 2 tba) in the aq... more Thiourea (Tu) crystallizes with barbituric acid (H 2 ba) and 2-thiobarbituric (H 2 tba) in the aqueous solution to yield co-crystals H 2 ba•Tu (1) and H 2 tba•Tu (2). Powder of 1 was also obtained from individual compounds via kneading with H 2 O. The structure of compounds was solved by the X-ray single crystal diffraction technique. In 1-2, N-H•••O, N-H•••S, C-H•••S and C-H•••O hydrogen bonds form the different 3D nets. In structure 1, centrosymmetric dimers of H 2 ba and Tu molecules are formed by two N-H•••O and N-H•••S hydrogen bonds, respectively. These dimers alternate in one-dimensional tapes. In compound 2, the same molecules are not bound by hydrogen bonds. Here, infinite chains are formed consisting of alternating molecules of H 2 tba and Tu. In these chains, each of the molecules is connected to the other by two N-H•••S hydrogen bonds. The compounds have been characterized by powder XRD, TG-DSC, and FT-IR.
Aaps Pharmscitech, 2008
The aim of this work was the development of extended release tablets of 500 mg of ciprofloxacin b... more The aim of this work was the development of extended release tablets of 500 mg of ciprofloxacin based on swellable drug polyelectrolyte matrices (SDPM). A set of complexes of carbomer, ciprofloxacin and sodium, (CB–Cip)50Na x , having a molar ratio Cip/CB acid groups of 0.5 and variable proportions of Na+ was used to prepare SDPM. Characterization of complexes by FT-IR, powder X-ray diffraction and thermal analysis revealed that Cip, in its protonated form, is ionically bonded to the functional groups of CB. Rates of fluid uptake of (CB–Cip)50Na x matrices as well as Cip release in simulated gastric fluid were modulated by changes in the proportion of Na+ incorporated in the complexes. A direct correlation between fluid uptake and delivery rate was observed along the series of matrices. Release rates were modulated from 1.4 mg/min to 25 mg/min in going from (CB–Cip)50Na10 to (CB–Cip)50Na14. The analysis of kinetic data suggest that rates of swelling, ionic pair dissociation and drug diffusion play a role in the kinetic control of delivery. Complexes were satisfactorily prepared and processed together with small amounts of antiadherent and lubricant excipients to obtain a series of extended release SDPM tablets through the current tableting technology processes. Cip release from matrices was widely modulated by the composition of the complexes yielding a flexible system that allows selecting a composition that releases in 120 min 90% of the dose in simulated gastric fluid.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 2003
A set of potential Class III antiarrhythmic agents of structure p-HOOC
Ear, nose, & throat journal, 2004
We conducted a prospective, randomized, controlled, double-blind study of 33 patients to compare ... more We conducted a prospective, randomized, controlled, double-blind study of 33 patients to compare the efficacy and tolerability of a new glycerin formulation of ototopical 0.3% ciprofloxacin with that of a conventional aqueous formulation of ciprofloxacin for the treatment of acute external otitis. Outcomes measures were resolution of discharge, swelling, pain, and redness and the incidence of adverse side effects. Patients were examined on three occasions: on the day of enrollment (visit 1), 48 to 72 hours later (visit 2), and 7 days after enrollment (visit 3). At visit 2, the patients in the glycerin group showed a significantly greater resolution of discharge. We observed the same pattern with respect to swelling, pain, and redness, which resolved more quickly in the glycerin group, although not significantly so. All patients were cured by visit 3, and the two treatments were equally well tolerated. On the basis of our findings, we conclude that the glycerin formulation of ototopical 0.3% ciprofloxacin appears to be at least as effective as the aqueous form in the treatment of acute external otitis-and in the case of otorrhea, more so.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, Jan 13, 2014
The low bioavailability of enalapril maleate associated to its instability in solid state motivat... more The low bioavailability of enalapril maleate associated to its instability in solid state motivated the development of a polyelectrolyte-drug complex between enalapril maleate and the cationic polymethacrylate Eudragit E100. The solid complexes were characterized by DSC-TG, FT-IR and X-ray diffraction. Their aqueous dispersions were evaluated for drug delivery in bicompartimental Franz cells and electrokinetic potentials. Stability in solid state was also evaluated using an HPLC-UV stability indicating method. Absorption of enalapril maleate was assessed thorough the rat everted gut sac model. In addition, urinary recovery after oral administration in rats was used as an indicator of systemic exposition. The solid materials are stable amorphous solids in which both moieties of enalapril maleate are ionically bonded to the polymer. Their aqueous dispersions exhibited controlled release over more than 7h in physiologic saline solution, being ionic exchange the fundamental mechanism that modified the extent and rate of drug release. Intestinal permeation of enalapril maleate was 1.7 times higher in the presence of the cationic polymer. This increase can be related with the capacity to adhere the mucosa due to the positive zeta potential of the complexes. As a consequence bioavailability was significantly improved (1.39 times) after oral administration of the complexes. In addition, no signs of chemical decomposition were observed after a 14months period. The results indicated that the products are new chemical entities that improve unfavorable properties of a useful drug.
Engineering Materials, 2014
Livestock Science, 2012
The present study investigated the effect of three different dietary fats [saturated fat (SAT) (3... more The present study investigated the effect of three different dietary fats [saturated fat (SAT) (3.1% TMR DM); linseed oil (LINA) (2.7% TMR DM) or sunflower oil (SUNA) (2.7% TMR DM) added with docosahexaenoic acid rich algae (0.4% TMR DM)] on SREBP-1 protein expression in mammary gland tissue of German Holstein cows (n= 18) in the context of milk yield, milk fat content and milk fatty acid composition. Prerequisite for this investigation was the development of a Western blot methodology adapted for bovine mammary gland tissue using two different primary antibodies to specifically detect and differentiate between precursor (pSREBP-1, 125 kDa) and transcriptionally active SREBP-1 (mSREBP-1, 68 kDa). Upon a ten-week feeding intervention, significantly reduced milk fat contents, total saturated fatty acid amounts and single saturated fatty acid concentrations (C10:0, C12:0, C14:0, C16:0) were obtained in LINA and SUNA group, whereas milk yields did not substantially differ between feeding groups. The assumption that the effects of plant oil/DHA rich algae supplementation on milk fat composition were mediated by shifted SREBP-1 protein expression were only confirmed in regard to slightly reduced protein expression levels of pSREBP-1 in SUNA group tissue samples (P = 0.087), whereas similar protein expression levels of transcriptionally active mSREBP-1 were found in SAT, LINA and SUNA group.
Background: In the hospital setting is frequent to manipulate solid dosage forms (SDF, tablets 16... more Background: In the hospital setting is frequent to manipulate solid dosage forms (SDF, tablets 16and capsules), which can affect their physicochemical and biopharmaceutical properties, the pharmacoloeffect and sometimes to cause the appearance of undesirable side effects. Objectives: To identify the medicines whose SDF is altered in a Hospital of Cordoba (Argentina), to determine how these modifications are made and to establish whether they were properly performed or not. Methods: We retrospectively analyzed the prescriptions made between the 5th and the 11th of March of 2012 and identified impaired requesting SDF modifications. Open interviews were held with nurses and service visits to each hospital to determine how they manipulated the SDF. Results: We analyzed 700 prescriptions for 113 patients, of which 61 (54%) had manipulations of the received SDF (49 for medical prescription, 7 because they had nasogastric tubes, 3 due to patient choice and 2 by nursing decision). Twenty th...
Revista de la Facultad de Ciencias Médicas (Córdoba, Argentina), 2013
In the hospital setting is frequent to manipulate solid dosage forms (SDF, tablets 16 and capsule... more In the hospital setting is frequent to manipulate solid dosage forms (SDF, tablets 16 and capsules), which can affect their physicochemical and biopharmaceutical properties, the pharmacoloeffect and sometimes to cause the appearance of undesirable side effects. To identify the medicines whose SDF is altered in a Hospital of Cordoba (Argentina), to determine how these modifications are made and to establish whether they were properly performed or not. We retrospectively analyzed the prescriptions made between the 5th and the 11th of March of 2012 and identified impaired requesting SDF modifications. Open interviews were held with nurses and service visits to each hospital to determine how they manipulated the SDF. We analyzed 700 prescriptions for 113 patients, of which 61 (54%) had manipulations of the received SDF (49 for medical prescription, 7 because they had nasogastric tubes, 3 due to patient choice and 2 by nursing decision). Twenty three medicines were manipulated, but only ...
Scientific Reports
The development of new treatments capable of controlling infections and pain related to burns con... more The development of new treatments capable of controlling infections and pain related to burns continues to be a challenge. Antimicrobials are necessary tools, but these can be cytotoxic for regenerating cells. In this study, antibiotic-anesthetic (AA) smart systems obtained by ionic complexation of polyelectrolytes with ciprofloxacin and lidocaine were obtained as films and hydrogels. Ionic complexation with sodium alginate and hyaluronate decreased cytotoxicity of ciprofloxacin above 70% in a primary culture of isolated fibroblasts (p < 0.05). In addition, the relative levels of the proteins involved in cell migration, integrin β1 and p-FAK, increased above 1.5 times (p < 0.05) with no significant differences in cell mobility. Evaluation of the systems in a deep second-degree burn model revealed that reepithelization rate was AA-films = AA-hydrogels > control films > no treated > reference cream (silver sulfadiazine cream). In addition, appendage conservation and com...
Purpose: To evaluate the stability of an extemporaneously prepared 7% chloral hydrate syrup under... more Purpose: To evaluate the stability of an extemporaneously prepared 7% chloral hydrate syrup under different conditions of storage and dispensing. Methods: Three batches of 7% chloral hydrate syrup were prepared. Each batch was stored in 50 light-resistant glass containers of 60 mL with child-resistant caps and in two bottles of 1000 mL to simulate two forms of dispensing, mono and multi-dose, respectively. Twenty five mono-dose bottles and a multi-dose bottle of each batch were stored under room conditions (20 ± 1°C) and the rest of the samples were stored in the fridge (5 ± 2°C). The physical, chemical and microbiological stability was evaluated for 180 days. Stability was defined as retention of at least 95% of the initial concentration of chloral hydrate, the absence of both visible particulate matter, or color and/or odor changes and the compliance with microbiological attributes of non-sterile pharmaceutical products. Results: At least 98% of the initial chloral hydrate concent...
Fil: Olivera, Maria Eugenia. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Arge... more Fil: Olivera, Maria Eugenia. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas; Argentina.
Pharmacy Education, 2018
The need to know the current state of professional practice in Argentina in different areas, and ... more The need to know the current state of professional practice in Argentina in different areas, and the absence of systematic information about it, led to the organisation of a workshop with pharmacists from several different practice settings. The workshop provided a space for reflection and dialogue for the participants committed to improving pharmacy education and professional practice in Argentina. The objective of this report was to document the main points from this workshop; which allowed the participants to reinforce their identity as pharmacists, review health policies in Argentina and identify the strengths and weaknesses of pharmacy education and professional practice in order to work on the gaps observed. A list of future actions to be implemented in the work setting was drawn up and the representatives who would allow the implementation of necessary action plans and who expressed their commitment to act upon them were identified. This experience highlighted the need to str...
Diabetes Mellitus is a chronic disease related to hyperglycaemia, and its treatment purposes are ... more Diabetes Mellitus is a chronic disease related to hyperglycaemia, and its treatment purposes are preventing and reducing the complications which cause more than 70% of deaths. Health programs with drug supply at public health facilities in Alta Gracia City were analized: Hospital Arturo Umberto Illia (HAUI), depending on the Health Ministry of the Province of Cordoba, and 12 dispensaries, depending on the Direccion de Salud Publica (DSP) of the Municipality of Alta Gracia. A total of 540 patients were identified in the merged data base and 49 were found in both settings. Drug consumptions, expressed in DDD per 100 patient under program by day, were: 25.2 in HAUI and 24.6 in DSP for Glibenclamide; 17.6 in HAUI and 13.1 in DSP for Metformin; 59.0 in HAUI for Insulin. The information obtained from the Pharmacy Services let the development of a diabetic patient data base and a consumption drug utilization study.
Objetivos: Describir las caracteristicas de la poblacion de pacientes diabeticos atendida en el s... more Objetivos: Describir las caracteristicas de la poblacion de pacientes diabeticos atendida en el subsector publico de salud de la ciudad de Alta Gracia. Analizar el uso de la cantidad de dispensaciones mensuales como indicador de adherencia al tratamiento, comparando resultados entre establecimientos sanitarios. Valorar la coordinacion de efectores publicos en la atencion de pacientes. Materiales y metodos: Diseno: estudio observacional, descriptivo-analitico. Lugar: Hospital Arturo Umberto Illia (HAUI) y Direccion de Salud Publica (DSP). Alta Gracia (Provincia de Cordoba, Argentina). Poblacion: Pacientes diabeticos pertenecientes a programas de salud de efectores publicos. Mediciones principales: Variables demograficas y epidemiologicas, cantidad de dispensaciones mensuales, adherencia global porcentual y coordinacion de efectores. Resultados: Del total de pacientes diabeticos (n=540): 52% se atendian en el HAUI, 39% en la DSP y 9% en ambos centros; 55% eran mujeres y la edad promed...
Ciências da Saúde: desafios, perspectivas e possibilidades - Volume 1, 2021
Cutaneous leishmaniasis (CL) is a neglected tropical disease endemic in ~90 countries, with an in... more Cutaneous leishmaniasis (CL) is a neglected tropical disease endemic in ~90 countries, with an increasing incidence. Presently available pharmacotherapy implies the systemic administration of moderately/very toxic drugs. Miltefosine (Milt) is the only FDA approved drug to treat CL via the oral route (Impavido®). It produces side effects; in particular, teratogenic effects are of concern. A topical treatment would have the great advantage of minimising the systemic circulation of the drug, preventing side effects. We prepared dispersions containing Milt and liposomes to modulate trans-epidermal penetration and evaluated in vivo efficacy. Treatments were topically administered to BALB/c mice infected with Leishmania (Leishmania) amazonensis. The dispersions containing 0.5 % Milt eliminated 99 % of the parasites and cured the lesions with a complete re-epithelisation, no visible scar and re-growth of hair. Fluid liposomes decreased the time to heal the lesion and the time needed to eliminate viable amastigotes from the lesion site. Relapse of the infection was not found one month after treatment in any case. A topical Milt formulation including fluid liposomes seems a promising treatment against CL.
Revista de la Facultad de Ciencias Medicas (Cordoba, Argentina), Dec 26, 2018
Plant extracts can be obtained to carry bioactive compounds, useful for prevention and treatment ... more Plant extracts can be obtained to carry bioactive compounds, useful for prevention and treatment of different illnesses. This also supports the intake of teas as functional beverages. Nonetheless, it is incompletely known whether these extracts can act as effective sources and vehicles de phenolic compounds (phenolics/polyphenols) to reach their targets. To establish whether phytoextract ingestion modified in a sex-dependent manner the phenolic bioavailability and redox response in liver and kidney. BALB/C mice ingested for a month 100 mg/Kg/d of extracts (tea-like) from Aspidosperma quebracho-blanco (AQB), Lantana grisebachii (LG) or Ilex paraguariensis (IP). Then, phenolics, peroxides and nitrites were analyzed by spectrophotometry. Also, phenolic permeation from digested and undigested extracts was evaluated in vitro with a rat jejunum-based assay. Phenolic permeation depended on extract digestion. In males, IP showed a special time course of hepatic phenolics, whereas all extrac...
European Journal of Pharmaceutical Sciences, 2017
One of the main obstacles to the successful treatment of tuberculosis is the poor and variable or... more One of the main obstacles to the successful treatment of tuberculosis is the poor and variable oral bioavailability of rifampicin (RIF), which is mainly due to its low hydrophilicity and dissolution rate. The aim of this work was to obtain a hydrophilic new material that allows a very fast dissolution rate of RIF and therefore is potentially useful in the development of oral solid dosage forms. The acid form of carboxymethylcellulose (CMC) was co-processed with RIF by solvent impregnation to obtain CMC-RIF powder, which was characterized by polarized optical microscopy, powder x-ray diffraction, DSC-TGA, hot stage microscopy, 13 C and 15 N solid-state NMR and FT-IR spectroscopy. In addition, the CMC-RIF matrices were subjected to water uptake and dissolution studies to assess hydrophilicity and release kinetics. CMC-RIF is a crystalline solid dispersion. Solid-state characterization indicated that no ionic interaction occurred between the components, but RIF crystallized as a zwitterion over the surface of CMC, which drastically increased the hydrophilicity of the solid. The CMC-RIF matrices significantly improved the water uptake of RIF and disintegrated in a very short period immediately releasing RIF. As CMC improves the hydrophilicity and delivery properties of RIF, CMC-RIF is very useful in the design of oral solid dosage forms with very fast dissolution of RIF, either alone or in combination with other antitubercular drugs.
Results in pharma sciences, 2016
The United States Pharmacopeia (USP) General Chapters Dissolution 〈711〉 and Disintegration and Di... more The United States Pharmacopeia (USP) General Chapters Dissolution 〈711〉 and Disintegration and Dissolution of Dietary Supplements 〈2040〉 allows the use of enzymes in dissolution media when gelatin capsules do not conform to dissolution specifications due to cross linking. Possible interactions between enzymes and surfactants when used together in dissolution media could result in loss of the enzymatic activity. Pepsin is an enzyme commonly used in dissolution media, and in this work, the activity of pepsin was determined in the presence of different surfactants as usually found in case of dissolution tests of certain gelatin capsule formulations. Pepsin enzymatic activity was determined according to the Ninth Edition of the Food Chemicals Codex (FCC) 9 method, in dissolution conditions: simulated gastric fluid, 37 °C and 50 rpm. Sodium dodecyl sulfate (SDS), cetyltrimethyl ammonium bromide (CTAB), polysorbate 80 (Tween 80) and octoxynol 9 (Triton X100) in concentrations above and be...
Farmacia hospitalaria : órgano oficial de expresión científica de la Sociedad Española de Farmacia Hospitalaria
To evaluate the stability of an extemporaneously prepared 7% chloral hydrate syrup under differen... more To evaluate the stability of an extemporaneously prepared 7% chloral hydrate syrup under different conditions of storage and dispensing. Three batches of 7% chloral hydrate syrup were prepared. Each batch was stored in 50 light-resistant glass containers of 60 mL with child-resistant caps and in two bottles of 1000 mL to simulate two forms of dispensing, mono and multi-dose, respectively. Twenty five mono-dose bottles and a multi-dose bottle of each batch were stored under room conditions (20 ± 1 °C) and the rest of the samples were stored in the fridge (5 ± 2 °C). The physical, chemical and microbiological stability was evaluated for 180 days. Stability was defined as retention of at least 95% of the initial concentration of chloral hydrate, the absence of both visible particulate matter, or color and/or odor changes and the compliance with microbiological attributes of non-sterile pharmaceutical products. At least 98% of the initial chloral hydrate concentration remained throughou...
International Journal of Pharmaceutics, 2010
Thiourea (Tu) crystallizes with barbituric acid (H 2 ba) and 2-thiobarbituric (H 2 tba) in the aq... more Thiourea (Tu) crystallizes with barbituric acid (H 2 ba) and 2-thiobarbituric (H 2 tba) in the aqueous solution to yield co-crystals H 2 ba•Tu (1) and H 2 tba•Tu (2). Powder of 1 was also obtained from individual compounds via kneading with H 2 O. The structure of compounds was solved by the X-ray single crystal diffraction technique. In 1-2, N-H•••O, N-H•••S, C-H•••S and C-H•••O hydrogen bonds form the different 3D nets. In structure 1, centrosymmetric dimers of H 2 ba and Tu molecules are formed by two N-H•••O and N-H•••S hydrogen bonds, respectively. These dimers alternate in one-dimensional tapes. In compound 2, the same molecules are not bound by hydrogen bonds. Here, infinite chains are formed consisting of alternating molecules of H 2 tba and Tu. In these chains, each of the molecules is connected to the other by two N-H•••S hydrogen bonds. The compounds have been characterized by powder XRD, TG-DSC, and FT-IR.
Aaps Pharmscitech, 2008
The aim of this work was the development of extended release tablets of 500 mg of ciprofloxacin b... more The aim of this work was the development of extended release tablets of 500 mg of ciprofloxacin based on swellable drug polyelectrolyte matrices (SDPM). A set of complexes of carbomer, ciprofloxacin and sodium, (CB–Cip)50Na x , having a molar ratio Cip/CB acid groups of 0.5 and variable proportions of Na+ was used to prepare SDPM. Characterization of complexes by FT-IR, powder X-ray diffraction and thermal analysis revealed that Cip, in its protonated form, is ionically bonded to the functional groups of CB. Rates of fluid uptake of (CB–Cip)50Na x matrices as well as Cip release in simulated gastric fluid were modulated by changes in the proportion of Na+ incorporated in the complexes. A direct correlation between fluid uptake and delivery rate was observed along the series of matrices. Release rates were modulated from 1.4 mg/min to 25 mg/min in going from (CB–Cip)50Na10 to (CB–Cip)50Na14. The analysis of kinetic data suggest that rates of swelling, ionic pair dissociation and drug diffusion play a role in the kinetic control of delivery. Complexes were satisfactorily prepared and processed together with small amounts of antiadherent and lubricant excipients to obtain a series of extended release SDPM tablets through the current tableting technology processes. Cip release from matrices was widely modulated by the composition of the complexes yielding a flexible system that allows selecting a composition that releases in 120 min 90% of the dose in simulated gastric fluid.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 2003
A set of potential Class III antiarrhythmic agents of structure p-HOOC
Ear, nose, & throat journal, 2004
We conducted a prospective, randomized, controlled, double-blind study of 33 patients to compare ... more We conducted a prospective, randomized, controlled, double-blind study of 33 patients to compare the efficacy and tolerability of a new glycerin formulation of ototopical 0.3% ciprofloxacin with that of a conventional aqueous formulation of ciprofloxacin for the treatment of acute external otitis. Outcomes measures were resolution of discharge, swelling, pain, and redness and the incidence of adverse side effects. Patients were examined on three occasions: on the day of enrollment (visit 1), 48 to 72 hours later (visit 2), and 7 days after enrollment (visit 3). At visit 2, the patients in the glycerin group showed a significantly greater resolution of discharge. We observed the same pattern with respect to swelling, pain, and redness, which resolved more quickly in the glycerin group, although not significantly so. All patients were cured by visit 3, and the two treatments were equally well tolerated. On the basis of our findings, we conclude that the glycerin formulation of ototopical 0.3% ciprofloxacin appears to be at least as effective as the aqueous form in the treatment of acute external otitis-and in the case of otorrhea, more so.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, Jan 13, 2014
The low bioavailability of enalapril maleate associated to its instability in solid state motivat... more The low bioavailability of enalapril maleate associated to its instability in solid state motivated the development of a polyelectrolyte-drug complex between enalapril maleate and the cationic polymethacrylate Eudragit E100. The solid complexes were characterized by DSC-TG, FT-IR and X-ray diffraction. Their aqueous dispersions were evaluated for drug delivery in bicompartimental Franz cells and electrokinetic potentials. Stability in solid state was also evaluated using an HPLC-UV stability indicating method. Absorption of enalapril maleate was assessed thorough the rat everted gut sac model. In addition, urinary recovery after oral administration in rats was used as an indicator of systemic exposition. The solid materials are stable amorphous solids in which both moieties of enalapril maleate are ionically bonded to the polymer. Their aqueous dispersions exhibited controlled release over more than 7h in physiologic saline solution, being ionic exchange the fundamental mechanism that modified the extent and rate of drug release. Intestinal permeation of enalapril maleate was 1.7 times higher in the presence of the cationic polymer. This increase can be related with the capacity to adhere the mucosa due to the positive zeta potential of the complexes. As a consequence bioavailability was significantly improved (1.39 times) after oral administration of the complexes. In addition, no signs of chemical decomposition were observed after a 14months period. The results indicated that the products are new chemical entities that improve unfavorable properties of a useful drug.
Engineering Materials, 2014