Bruce Stillman | Cold Spring Harbor Laboratory (original) (raw)
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National Museum of Natural History, Bulgarian Academy of Science
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Papers by Bruce Stillman
Cell, 1995
The origin recognition complex (ORC) binds replicators in the yeast S. cerevisiae in a manner con... more The origin recognition complex (ORC) binds replicators in the yeast S. cerevisiae in a manner consistent with it being an initiator protein for DNA replication. Two-dimensional (2D) gel techniques were used to examine directly initiation of chromosomal DNA replication in temperature-sensitive otc mutants. Unlike in wild-type cells, in orc2.1 and orc5.1 mutant cells, only a subset of replicators formed active origins of DNA replication at the permissive temperature. At the restrictive temperature, the number of active replicators was diminished further. Using a genetic screen, CDC6 was identified as a multicopy suppressor of orc5.1. 2D gel and biochemical analyses demonstrated that Cdc6p interacted functionally and physically with ORC. We suggest that ORC and Cdc6p form a prereplication complex at individual replicators and therefore cooperate to determine the frequency of initiation of DNA replication in the genome.
Proceedings of the National Academy of Sciences, 1995
Replicators are genetically defined elements within chromosomes that determine the location of or... more Replicators are genetically defined elements within chromosomes that determine the location of origins of DNA replication. In the yeast Saccharomyces cerevisiae, the ARSI replicator contains multiple functional DNA elements: an essential A element and three important B elements-Bi, B2, and B3. Functionally similar A, Bi, and B2 elements are also present in the ARS307 replicator. The B3 element binds a replication and transcription enhancer protein Abftp, whereas the A element is required for binding the origin recognition complex (ORC). The function of the Bi and B2
The Embo Journal, Jul 15, 1994
2Corresponding author Communicated by T.Lindahl
Cell, 1995
The origin recognition complex (ORC) binds replicators in the yeast S. cerevisiae in a manner con... more The origin recognition complex (ORC) binds replicators in the yeast S. cerevisiae in a manner consistent with it being an initiator protein for DNA replication. Two-dimensional (2D) gel techniques were used to examine directly initiation of chromosomal DNA replication in temperature-sensitive otc mutants. Unlike in wild-type cells, in orc2.1 and orc5.1 mutant cells, only a subset of replicators formed active origins of DNA replication at the permissive temperature. At the restrictive temperature, the number of active replicators was diminished further. Using a genetic screen, CDC6 was identified as a multicopy suppressor of orc5.1. 2D gel and biochemical analyses demonstrated that Cdc6p interacted functionally and physically with ORC. We suggest that ORC and Cdc6p form a prereplication complex at individual replicators and therefore cooperate to determine the frequency of initiation of DNA replication in the genome.
Proceedings of the National Academy of Sciences, 1995
Replicators are genetically defined elements within chromosomes that determine the location of or... more Replicators are genetically defined elements within chromosomes that determine the location of origins of DNA replication. In the yeast Saccharomyces cerevisiae, the ARSI replicator contains multiple functional DNA elements: an essential A element and three important B elements-Bi, B2, and B3. Functionally similar A, Bi, and B2 elements are also present in the ARS307 replicator. The B3 element binds a replication and transcription enhancer protein Abftp, whereas the A element is required for binding the origin recognition complex (ORC). The function of the Bi and B2
The Embo Journal, Jul 15, 1994
2Corresponding author Communicated by T.Lindahl