Deborah Chen | California State University, Northridge (original) (raw)

Papers by Deborah Chen

This report identifies key issues for providing early childhood special education services to you... more This report identifies key issues for providing early childhood special education services to young children who are visually impaired and for working with families of culturally and linguistically diverse backgrounds. First, it discusses the incidence of visual impairment and associated disabilities among young children, the process of early identification, and various definitions related to visual impairment. Second, it reviews relevant literature of the effects of visual impairment and associated disabilities on early development. Third, it outlines current intervention practices in relationship to the Division of Early Childhood Recommended Practices (1993). Fourth, it presents implications for serving infants, toddlers, and preschoolers with visual impairments, those with associated disabilities, and their families. The report includes extensive references, an annotated bibliography, and a list of available resources from the CLAS Web site. (Author/SG) Reproductions supplied by EDRS are the best that can be made from the original document.

Young Exceptional Children, May 31, 2020

M s. Medina (a bilingual Spanish-English general education preschool teacher) and Mr. Young (a ce... more M s. Medina (a bilingual Spanish-English general education preschool teacher) and Mr. Young (a certified teacher in early childhood special education) co-teach a class of 20 children that includes six preschoolers who receive special education services. Because these two teachers have had very limited experience with children who have visual impairments, they wonder about how best to teach two newly enrolled preschoolers. Three-year-old Ximena has developmental delays, hearing loss, and visual impairment associated with Down syndrome. Four-year-old Sihan was born at 24 weeks gestation age and has a significant vision loss from retinopathy of prematurity (ROP) and mild cerebral palsy. Both classroom preschool teachers have met the following itinerant consultants: a certified teacher of students with visual impairments (TSVI) who is dually certified as an orientation and mobility specialist (COMS), a certified teacher of students who are deaf or hard of hearing (TSDHH), a speech and language pathologist (SLP), an occupational therapist (OT), and a physical therapist (PT). Ximena and Sihan receive related services through a push in model within classroom activities. The TSVI provides

Infants and Young Children, Jul 1, 1997

Journal of Visual Impairment & Blindness, Sep 1, 2010

Nutrients

Background: This study assesses the effectiveness of a campaign “Are We Drinking Ourselves Sick?”... more Background: This study assesses the effectiveness of a campaign “Are We Drinking Ourselves Sick?” that ran nationally in Jamaica in four phases from 2017 to 2019 to increase knowledge about the harms of sugary drinks, shift attitudes, and build support for policy actions to address sugary drink consumption, including a tax and a ban in schools. Methods: Campaign impact was measured in representative cross-sectional household surveys of adults ages 18 to 55. A baseline survey was conducted before the launch of the campaign (n = 1430). Evaluation surveys were conducted mid-campaign (n = 1571) and post-campaign (n = 1500). Campaign impact was assessed by comparing changes across survey periods on key knowledge, attitudinal and policy support outcome indicators. The independent association between campaign awareness and outcomes was analyzed using logistic regression analyses. Results: The campaign was recalled by more than 80% of respondents and was well-received with 90% or more respo...

Cancer Research, 2021

Oncogenic EGFR mutations occur in approximately 50% of glioblastomas (GBM) and largely reside in ... more Oncogenic EGFR mutations occur in approximately 50% of glioblastomas (GBM) and largely reside in the extracellular domain. Prior attempts to reposition current generation EGFR inhibitors to treat GBM likely failed due to poor brain penetration and an inability to potently target the full spectrum of oncogenic mutations. As EGFR oncogenic mutations are found to be co-expressed in many GBMs, it is important that an inhibitor be broadly active against the entire family of relevant EGFR mutants. Additionally, a successful inhibitor would require a pharmacokinetic (PK) profile that allows for sufficient penetration of the blood brain barrier to elicit robust target engagement of the brain tumor. Using these design principles, we designed a series of highly potent molecules exemplified by BDTX-507. This molecule is an irreversible inhibitor of EGFR with antiproliferative IC50's less than 10 nM against the spectrum of GBM-relevant EGFR mutations. PK/PD studies demonstrated sustained pE...

Journal of Ship Production and Design, 2010

Low enrollment and high attrition rates in science, technology, engineering, and math (STEM) base... more Low enrollment and high attrition rates in science, technology, engineering, and math (STEM) based degree programs have created a shortage of workforce in industries such as shipbuilding and repair that are important for national security. Part of this problem can be attributed to pedagogical issues such as lack of engaging hands-on activities used for math and science instruction in middle and high schools. Another reason for this is that the teachers are not trained in taking an integrated approach to teaching math and science. This has led to large-scale flight and attrition from STEM-based career tracks. Consequently, engineering and engineering technology programs throughout the nation have observed declining graduation rates and quality of incoming students. To engage students' interest in STEM-based careers, it is important that students establish a link between the math and science instruction and its application to solve real-life problems early in their learning experi...

Transfusion, 2019

BACKGROUND: The application of riboflavin/UV-based pathogen inactivation (PI) to whole blood (WB)... more BACKGROUND: The application of riboflavin/UV-based pathogen inactivation (PI) to whole blood (WB) is currently limited by its negative impact on red blood cell (RBC) quality. The generation of reactive oxidative species in RBC products contributes to increased hemolysis. This study evaluated the impact of deoxygenation of WB prior to riboflavin/UV light treatment versus deoxygenation of RBC concentrates after PI treatment by monitoring RBC in vitro quality parameters. STUDY DESIGN AND METHODS: Six ABO-matched WB units were pooled and split. Within three pairs, one unit was treated with riboflavin/UV light while the other was kept as an untreated control prior to manufacture into red cell concentrates (RCCs). The first pair (Cntr; Cntr-PI) served as the normoxic controls. Deoxygenation was performed at the RCC level for the second pair (RCCdeox; PI-RCCdeox), and at the WB level of the third pair (WBdeox; WBdeox-PI). In vitro qualities of the respective RBC units were assessed throughout storage. RESULTS: The data for the Cntr and Cntr-PI units were comparable to previous reports. The PI-RCCdeox units exhibited worse in vitro quality for most parameters tested compared to Cntr-PI and WBdeox-PI units throughout storage. Hemolysis and microvesicle release was significantly (p < 0.05) higher on Days 21 and 42 in Cntr-PI units compared to WBdeox-PI units. CONCLUSION: WB deoxygenation may help to decrease the accelerated deterioration in RCC in vitro quality caused by treatment with riboflavin/UV light. Treatment of WB under reduced oxygen levels needs to be assessed for PI effectiveness.

Computers & Education, 2019

Bioorganic & Medicinal Chemistry Letters, 2018

We report here the design and synthesis of a novel series of benzylamines that are potent and sel... more We report here the design and synthesis of a novel series of benzylamines that are potent and selective inhibitors of uPA with promising oral availability in rat. Further evaluation of one representative (ZK824859) of the new structural class showed that this compound lowered clinical scores when dosed in either acute or chronic mouse EAE models, suggesting that uPA inhibitors of this type could be useful for the treatment of multiple sclerosis.

International Journal of Technology Assessment in Health Care, 2018

Introduction:The best available research and funding policy evidence regarding the regulatory sta... more Introduction:The best available research and funding policy evidence regarding the regulatory status, patient selection criteria, safety, clinical effectiveness, and financial impact of peroral endoscopic myotomy (POEM) for the treatment of achalasia was synthesized for statewide decision making for a South Australian local health network.Methods:A comprehensive systematic search of twenty-three gray literature sources and three published literature databases for international evidence was conducted, based on a priori inclusion criteria. The methodological quality of the included studies was critically appraised. Data extraction and synthesis were conducted in narrative form.Results:Short-term safety and clinical data from very low-level studies showed that POEM appears to be a relatively safe and clinically effective endoscopic treatment for esophageal achalasia, compared with laparoscopic Heller myotomy (LHM). One primary safety concern consistently highlighted by the literature w...

Heart Failure Reviews, 2018

Acute heart failure (AHF) is a major burden disease, with a complex physiopathology, unsatisfacto... more Acute heart failure (AHF) is a major burden disease, with a complex physiopathology, unsatisfactory diagnosis, treatment and a very poor prognosis. In the last two decades, a number of drugs have progressed from preclinical to early and late clinical development, but only a few of them have been approved and added to a stagnant pharmacological armamentarium. We have reviewed the data published on drugs developed for AHF since early 2000s, trying to recognise factors that have worked for a successful approval or for the stoppage of the program, in an attempt to delineate future trajectories for AHF drug development. Our review has identified limitations at both preclinical and clinical levels. At the preclinical level, the major shortcoming is represented by animal models looking at short-term endpoints which do not recapitulate the complexity of the human disease. At the clinical level, the main weakness is given by the disconnect between short-term endpoints assessed in the early stage of drug development, and medium-long-term endpoints requested in Phase 3 for regulatory approval. This is further amplified by the lack of validation and standardisation of short-and long-term endpoints; absence of predictive biomarkers; conduct of studies on heterogeneous populations; and use of different eligibility criteria, time of assessments, drug schedules and background therapies. Key goals remain a better understanding of AHF and the construction of a successful drug development program. A reasonable way to move forward resides in a strong collaboration between main stakeholders of therapeutic innovation: scientific community, industry and regulatory agencies. Keywords Acute heart failure. Clinical trials. Study endpoints. Drug development. Proof of mechanism. Proof of principle .

International urology and nephrology, Jan 26, 2018

This article updates the qualitative research on Iran reported in the 2012 article by Tong et al.... more This article updates the qualitative research on Iran reported in the 2012 article by Tong et al. "The experiences of commercial kidney donors: thematic synthesis of qualitative research" (Tong et al. in Transpl Int 25:1138-1149, 2012). The basic approach used in the Tong et al. article is applied to a more recent and more comprehensive study of Iranian living organ donors, providing a clearer picture of what compensated organ donation is like in Iran since the national government began regulating compensated donation. Iran is the only country in the world where kidney selling is legal, regulated, and subsidized by the national government. This article focuses on three themes: (1) coercion and other pressures to donate, (2) donor satisfaction with their donation experience, and (3) whether donors fear social stigma. We found no evidence of coercion, but 68% of the paid living organ donors interviewed felt pressure to donate due to extreme poverty or other family pressures....

Transfusion, Sep 20, 2017

The development of hemolysis during ex vivo hypothermic storage is multifaceted. Standardization ... more The development of hemolysis during ex vivo hypothermic storage is multifaceted. Standardization of collection and production processes is used to minimize variability in biologics manufacturing and to maximize product quality. However, the influence of various donor characteristics on product quality is often difficult to evaluate and to control. Using a proteomic approach, we aimed to decipher relevant donor characteristics that may predict red blood cell (RBC) quality during storage. Ten healthy volunteer donors exhibiting repeated high hemolysis at outdate (>0.8%; RBC(HH) ) and 10 age- and sex-matched control donors (RBC(Ctrl) ) were studied. Common quality variables were measured on Days 5, 14, 21, 28, and 42 of storage. Protein profiles of hemoglobin-depleted membrane fractions from RBC(HH) and RBC(Ctrl) donors were analyzed using a quantitative proteomics approach based on iTRAQ (isobaric tags for relative and absolute quantitation). Time-dependent lesion development was a...

Transfusion, Mar 26, 2016

Pathogen reduction treatment using riboflavin and ultraviolet light illumination (Mirasol) effect... more Pathogen reduction treatment using riboflavin and ultraviolet light illumination (Mirasol) effectively reduces the risk of transfusion-transmitted infections. This treatment is currently licensed for only platelets and plasma products, while its application to whole blood (WB) to generate pathogen-inactivated red blood cells (RBCs) is under development. RBC storage lesion, constituting numerous morphologic and biochemical changes, influences RBC quality and limits shelf life. Stored RBCs further show enhanced susceptibility to RBC programmed cell death (eryptosis) characterized by increased cytosolic Ca(2+) -provoked membrane phosphatidylserine (PS) externalization. Using a "pool-and-split" approach, we examined multiple variables of RBC storage lesion and eryptosis in RBC units, derived from Mirasol-treated or untreated WB, after 4 to 42 days of storage, under blood bank conditions. In comparison to untreated RBC units, Mirasol treatment significantly altered membrane mic...

Proteomics. Clinical applications, Jul 13, 2017

Post-collection manipulations (PCMs) aim to increase blood product safety. However, PCMs improve ... more Post-collection manipulations (PCMs) aim to increase blood product safety. However, PCMs improve safety at a cost to quality, causing elevated hemolysis. As hemolysis is linked to red blood cell membrane integrity, a quantitative proteomics approach was employed to assess membrane proteome alterations induced by PCMs. Three ABO-matched whole blood (WB) units were pooled-and-split into three identical units. One WB unit was treated with riboflavin/ultraviolet illumination prior to red cell concentrate (RCC) production (RCC(WB*) ). Two WB units were produced into RCC; one was gamma-irradiated (RCC(γ) ) and the other was left untreated as control (RCC(Ø) ). In vitro quality parameters were measured during storage. Membrane protein profiles of RCC(Ø) , RCC(γ) , and RCC(WB*) were assessed on selected hemoglobin-depleted membrane fractions using a quantitative proteomics approach based on iTRAQ. Quantitative proteomic analysis identified 100 proteins at the membrane, with seven unique pro...

The Analyst, Jan 23, 2016

Individual units of donated red blood cells (RBCs) do not ordinarily undergo analytical testing p... more Individual units of donated red blood cells (RBCs) do not ordinarily undergo analytical testing prior to transfusion. This study establishes the utility of Raman spectroscopy for analyzing the biochemistry of stored RBC supernatant and reveals interesting storage-related changes about the accumulation of lactate, a chemical species that may be harmful to certain patients. The data show measurable variations in supernatant composition and demonstrate that some units of donated RBCs accumulate lactate much more readily than others. The spectra also indicate a higher relative concentration of lactate in units collected from male donors than female donors (p = 0.004) and imply that there is a greater degree of variability at later stages of storage in units from older male donors (>45 years). The study proves that Raman analysis has promise for elucidating the relationship between the metabolism of stored RBCs and donor characteristics. It also suggests that there may be benefit in d...

This report identifies key issues for providing early childhood special education services to you... more This report identifies key issues for providing early childhood special education services to young children who are visually impaired and for working with families of culturally and linguistically diverse backgrounds. First, it discusses the incidence of visual impairment and associated disabilities among young children, the process of early identification, and various definitions related to visual impairment. Second, it reviews relevant literature of the effects of visual impairment and associated disabilities on early development. Third, it outlines current intervention practices in relationship to the Division of Early Childhood Recommended Practices (1993). Fourth, it presents implications for serving infants, toddlers, and preschoolers with visual impairments, those with associated disabilities, and their families. The report includes extensive references, an annotated bibliography, and a list of available resources from the CLAS Web site. (Author/SG) Reproductions supplied by EDRS are the best that can be made from the original document.

Young Exceptional Children, May 31, 2020

M s. Medina (a bilingual Spanish-English general education preschool teacher) and Mr. Young (a ce... more M s. Medina (a bilingual Spanish-English general education preschool teacher) and Mr. Young (a certified teacher in early childhood special education) co-teach a class of 20 children that includes six preschoolers who receive special education services. Because these two teachers have had very limited experience with children who have visual impairments, they wonder about how best to teach two newly enrolled preschoolers. Three-year-old Ximena has developmental delays, hearing loss, and visual impairment associated with Down syndrome. Four-year-old Sihan was born at 24 weeks gestation age and has a significant vision loss from retinopathy of prematurity (ROP) and mild cerebral palsy. Both classroom preschool teachers have met the following itinerant consultants: a certified teacher of students with visual impairments (TSVI) who is dually certified as an orientation and mobility specialist (COMS), a certified teacher of students who are deaf or hard of hearing (TSDHH), a speech and language pathologist (SLP), an occupational therapist (OT), and a physical therapist (PT). Ximena and Sihan receive related services through a push in model within classroom activities. The TSVI provides

Infants and Young Children, Jul 1, 1997

Journal of Visual Impairment & Blindness, Sep 1, 2010

Nutrients

Background: This study assesses the effectiveness of a campaign “Are We Drinking Ourselves Sick?”... more Background: This study assesses the effectiveness of a campaign “Are We Drinking Ourselves Sick?” that ran nationally in Jamaica in four phases from 2017 to 2019 to increase knowledge about the harms of sugary drinks, shift attitudes, and build support for policy actions to address sugary drink consumption, including a tax and a ban in schools. Methods: Campaign impact was measured in representative cross-sectional household surveys of adults ages 18 to 55. A baseline survey was conducted before the launch of the campaign (n = 1430). Evaluation surveys were conducted mid-campaign (n = 1571) and post-campaign (n = 1500). Campaign impact was assessed by comparing changes across survey periods on key knowledge, attitudinal and policy support outcome indicators. The independent association between campaign awareness and outcomes was analyzed using logistic regression analyses. Results: The campaign was recalled by more than 80% of respondents and was well-received with 90% or more respo...

Cancer Research, 2021

Oncogenic EGFR mutations occur in approximately 50% of glioblastomas (GBM) and largely reside in ... more Oncogenic EGFR mutations occur in approximately 50% of glioblastomas (GBM) and largely reside in the extracellular domain. Prior attempts to reposition current generation EGFR inhibitors to treat GBM likely failed due to poor brain penetration and an inability to potently target the full spectrum of oncogenic mutations. As EGFR oncogenic mutations are found to be co-expressed in many GBMs, it is important that an inhibitor be broadly active against the entire family of relevant EGFR mutants. Additionally, a successful inhibitor would require a pharmacokinetic (PK) profile that allows for sufficient penetration of the blood brain barrier to elicit robust target engagement of the brain tumor. Using these design principles, we designed a series of highly potent molecules exemplified by BDTX-507. This molecule is an irreversible inhibitor of EGFR with antiproliferative IC50's less than 10 nM against the spectrum of GBM-relevant EGFR mutations. PK/PD studies demonstrated sustained pE...

Journal of Ship Production and Design, 2010

Low enrollment and high attrition rates in science, technology, engineering, and math (STEM) base... more Low enrollment and high attrition rates in science, technology, engineering, and math (STEM) based degree programs have created a shortage of workforce in industries such as shipbuilding and repair that are important for national security. Part of this problem can be attributed to pedagogical issues such as lack of engaging hands-on activities used for math and science instruction in middle and high schools. Another reason for this is that the teachers are not trained in taking an integrated approach to teaching math and science. This has led to large-scale flight and attrition from STEM-based career tracks. Consequently, engineering and engineering technology programs throughout the nation have observed declining graduation rates and quality of incoming students. To engage students' interest in STEM-based careers, it is important that students establish a link between the math and science instruction and its application to solve real-life problems early in their learning experi...

Transfusion, 2019

BACKGROUND: The application of riboflavin/UV-based pathogen inactivation (PI) to whole blood (WB)... more BACKGROUND: The application of riboflavin/UV-based pathogen inactivation (PI) to whole blood (WB) is currently limited by its negative impact on red blood cell (RBC) quality. The generation of reactive oxidative species in RBC products contributes to increased hemolysis. This study evaluated the impact of deoxygenation of WB prior to riboflavin/UV light treatment versus deoxygenation of RBC concentrates after PI treatment by monitoring RBC in vitro quality parameters. STUDY DESIGN AND METHODS: Six ABO-matched WB units were pooled and split. Within three pairs, one unit was treated with riboflavin/UV light while the other was kept as an untreated control prior to manufacture into red cell concentrates (RCCs). The first pair (Cntr; Cntr-PI) served as the normoxic controls. Deoxygenation was performed at the RCC level for the second pair (RCCdeox; PI-RCCdeox), and at the WB level of the third pair (WBdeox; WBdeox-PI). In vitro qualities of the respective RBC units were assessed throughout storage. RESULTS: The data for the Cntr and Cntr-PI units were comparable to previous reports. The PI-RCCdeox units exhibited worse in vitro quality for most parameters tested compared to Cntr-PI and WBdeox-PI units throughout storage. Hemolysis and microvesicle release was significantly (p < 0.05) higher on Days 21 and 42 in Cntr-PI units compared to WBdeox-PI units. CONCLUSION: WB deoxygenation may help to decrease the accelerated deterioration in RCC in vitro quality caused by treatment with riboflavin/UV light. Treatment of WB under reduced oxygen levels needs to be assessed for PI effectiveness.

Computers & Education, 2019

Bioorganic & Medicinal Chemistry Letters, 2018

We report here the design and synthesis of a novel series of benzylamines that are potent and sel... more We report here the design and synthesis of a novel series of benzylamines that are potent and selective inhibitors of uPA with promising oral availability in rat. Further evaluation of one representative (ZK824859) of the new structural class showed that this compound lowered clinical scores when dosed in either acute or chronic mouse EAE models, suggesting that uPA inhibitors of this type could be useful for the treatment of multiple sclerosis.

International Journal of Technology Assessment in Health Care, 2018

Introduction:The best available research and funding policy evidence regarding the regulatory sta... more Introduction:The best available research and funding policy evidence regarding the regulatory status, patient selection criteria, safety, clinical effectiveness, and financial impact of peroral endoscopic myotomy (POEM) for the treatment of achalasia was synthesized for statewide decision making for a South Australian local health network.Methods:A comprehensive systematic search of twenty-three gray literature sources and three published literature databases for international evidence was conducted, based on a priori inclusion criteria. The methodological quality of the included studies was critically appraised. Data extraction and synthesis were conducted in narrative form.Results:Short-term safety and clinical data from very low-level studies showed that POEM appears to be a relatively safe and clinically effective endoscopic treatment for esophageal achalasia, compared with laparoscopic Heller myotomy (LHM). One primary safety concern consistently highlighted by the literature w...

Heart Failure Reviews, 2018

Acute heart failure (AHF) is a major burden disease, with a complex physiopathology, unsatisfacto... more Acute heart failure (AHF) is a major burden disease, with a complex physiopathology, unsatisfactory diagnosis, treatment and a very poor prognosis. In the last two decades, a number of drugs have progressed from preclinical to early and late clinical development, but only a few of them have been approved and added to a stagnant pharmacological armamentarium. We have reviewed the data published on drugs developed for AHF since early 2000s, trying to recognise factors that have worked for a successful approval or for the stoppage of the program, in an attempt to delineate future trajectories for AHF drug development. Our review has identified limitations at both preclinical and clinical levels. At the preclinical level, the major shortcoming is represented by animal models looking at short-term endpoints which do not recapitulate the complexity of the human disease. At the clinical level, the main weakness is given by the disconnect between short-term endpoints assessed in the early stage of drug development, and medium-long-term endpoints requested in Phase 3 for regulatory approval. This is further amplified by the lack of validation and standardisation of short-and long-term endpoints; absence of predictive biomarkers; conduct of studies on heterogeneous populations; and use of different eligibility criteria, time of assessments, drug schedules and background therapies. Key goals remain a better understanding of AHF and the construction of a successful drug development program. A reasonable way to move forward resides in a strong collaboration between main stakeholders of therapeutic innovation: scientific community, industry and regulatory agencies. Keywords Acute heart failure. Clinical trials. Study endpoints. Drug development. Proof of mechanism. Proof of principle .

International urology and nephrology, Jan 26, 2018

This article updates the qualitative research on Iran reported in the 2012 article by Tong et al.... more This article updates the qualitative research on Iran reported in the 2012 article by Tong et al. "The experiences of commercial kidney donors: thematic synthesis of qualitative research" (Tong et al. in Transpl Int 25:1138-1149, 2012). The basic approach used in the Tong et al. article is applied to a more recent and more comprehensive study of Iranian living organ donors, providing a clearer picture of what compensated organ donation is like in Iran since the national government began regulating compensated donation. Iran is the only country in the world where kidney selling is legal, regulated, and subsidized by the national government. This article focuses on three themes: (1) coercion and other pressures to donate, (2) donor satisfaction with their donation experience, and (3) whether donors fear social stigma. We found no evidence of coercion, but 68% of the paid living organ donors interviewed felt pressure to donate due to extreme poverty or other family pressures....

Transfusion, Sep 20, 2017

The development of hemolysis during ex vivo hypothermic storage is multifaceted. Standardization ... more The development of hemolysis during ex vivo hypothermic storage is multifaceted. Standardization of collection and production processes is used to minimize variability in biologics manufacturing and to maximize product quality. However, the influence of various donor characteristics on product quality is often difficult to evaluate and to control. Using a proteomic approach, we aimed to decipher relevant donor characteristics that may predict red blood cell (RBC) quality during storage. Ten healthy volunteer donors exhibiting repeated high hemolysis at outdate (>0.8%; RBC(HH) ) and 10 age- and sex-matched control donors (RBC(Ctrl) ) were studied. Common quality variables were measured on Days 5, 14, 21, 28, and 42 of storage. Protein profiles of hemoglobin-depleted membrane fractions from RBC(HH) and RBC(Ctrl) donors were analyzed using a quantitative proteomics approach based on iTRAQ (isobaric tags for relative and absolute quantitation). Time-dependent lesion development was a...

Transfusion, Mar 26, 2016

Pathogen reduction treatment using riboflavin and ultraviolet light illumination (Mirasol) effect... more Pathogen reduction treatment using riboflavin and ultraviolet light illumination (Mirasol) effectively reduces the risk of transfusion-transmitted infections. This treatment is currently licensed for only platelets and plasma products, while its application to whole blood (WB) to generate pathogen-inactivated red blood cells (RBCs) is under development. RBC storage lesion, constituting numerous morphologic and biochemical changes, influences RBC quality and limits shelf life. Stored RBCs further show enhanced susceptibility to RBC programmed cell death (eryptosis) characterized by increased cytosolic Ca(2+) -provoked membrane phosphatidylserine (PS) externalization. Using a "pool-and-split" approach, we examined multiple variables of RBC storage lesion and eryptosis in RBC units, derived from Mirasol-treated or untreated WB, after 4 to 42 days of storage, under blood bank conditions. In comparison to untreated RBC units, Mirasol treatment significantly altered membrane mic...

Proteomics. Clinical applications, Jul 13, 2017

Post-collection manipulations (PCMs) aim to increase blood product safety. However, PCMs improve ... more Post-collection manipulations (PCMs) aim to increase blood product safety. However, PCMs improve safety at a cost to quality, causing elevated hemolysis. As hemolysis is linked to red blood cell membrane integrity, a quantitative proteomics approach was employed to assess membrane proteome alterations induced by PCMs. Three ABO-matched whole blood (WB) units were pooled-and-split into three identical units. One WB unit was treated with riboflavin/ultraviolet illumination prior to red cell concentrate (RCC) production (RCC(WB*) ). Two WB units were produced into RCC; one was gamma-irradiated (RCC(γ) ) and the other was left untreated as control (RCC(Ø) ). In vitro quality parameters were measured during storage. Membrane protein profiles of RCC(Ø) , RCC(γ) , and RCC(WB*) were assessed on selected hemoglobin-depleted membrane fractions using a quantitative proteomics approach based on iTRAQ. Quantitative proteomic analysis identified 100 proteins at the membrane, with seven unique pro...

The Analyst, Jan 23, 2016

Individual units of donated red blood cells (RBCs) do not ordinarily undergo analytical testing p... more Individual units of donated red blood cells (RBCs) do not ordinarily undergo analytical testing prior to transfusion. This study establishes the utility of Raman spectroscopy for analyzing the biochemistry of stored RBC supernatant and reveals interesting storage-related changes about the accumulation of lactate, a chemical species that may be harmful to certain patients. The data show measurable variations in supernatant composition and demonstrate that some units of donated RBCs accumulate lactate much more readily than others. The spectra also indicate a higher relative concentration of lactate in units collected from male donors than female donors (p = 0.004) and imply that there is a greater degree of variability at later stages of storage in units from older male donors (>45 years). The study proves that Raman analysis has promise for elucidating the relationship between the metabolism of stored RBCs and donor characteristics. It also suggests that there may be benefit in d...