Mostapha BRAHMI | Centre Universitare de Relizane ALGERIE (original) (raw)

Papers by Mostapha BRAHMI

Notulae Scientia Biologicae, 2021

The purpose of this study was to evaluate the prophylactic effect of Pimpinella anisum (green ani... more The purpose of this study was to evaluate the prophylactic effect of Pimpinella anisum (green anis) on neurobehavioral status following mercury chloride intoxication during the developmental period. For this purpose, rats exposed to 100 mg/L of HgCl2 during the gestation and lactation period. A group of rats was treated with the anis extract for 15 days before becoming intoxicated with mercury. In contrast, one group was orally administered aqueous anis extract for 15 days after intoxication. The forced swimming test, the open field test and the Morris pool respectively recorded an increase in immobility time, a decrease in the number of cross-cells (p <0.001), (p <0.05) and an increase in latency (p <0.01), (p <0.001), (p <0.001) and decreased time spent in the target frame during the probe test (p <0.01) and increased latency in the visible test (p <0.01) in HgCl2 - exposed rats compared to control rats. However, preventive and curative aniseed-based treatment...

Natural Product Research, 2024

Plants have gained great importance. Secondary metabolites contribute to the drug discovery and d... more Plants have gained great importance. Secondary metabolites contribute to the drug discovery and development by their bioactive properties. Rubia tinctorum L. essential oil (EO) was obtained and analysed. Antioxidant and antibacterial activities were evaluated. The plant's EOs were obtained through steam distillation, and the compounds were identified using gas chromatography-mass spectrometry (GC-MS) analysis. DPPH free radical scavenging and ferric-reducing antioxidant power (FRAP) were employed to assess antioxidant activity. Total antioxidant capacity (TAC) was also presented. The disc diffusion method was employed for testing antibacterial activity. Cyclohexanone was identified as the predominant component in the EO, constituting 88.74% of the total composition. The EO did not show significant antioxidant capacity, while it demonstrated antimicrobial effect against Bacillus cereus ATCC 6633 (>13 mm of inhibition; 500 mg/mL) and Shigella ATCC 12022 (≥12 mm of inhibition; 500 mg/mL). R. tinctorum L. is new source of cyclohexanone.

Fresenius Environmental Bulletin , 2023

This study aims to examine the effects induced by lead acetate (Pb) using different experimental ... more This study aims to examine the effects induced by lead acetate (Pb) using different experimental approaches, such as neurobehavioral, biochemical, and histological in young Wistar rats during gestation and lactation, and to test the probiotic effect of Lactobacillus rhamnosus (Prob) throughout correcting or not the harmful effects of the studied metal. Chronic exposure to 0.2% lead acetate in rats during the development period resulted in a decrease in body and brain weights compared to controls. Test results for depression (forced swimming test), anxiety (Elevated plus maze), and locomotor and explo ratory activity (Open field) all show that Pb intoxication causes neurobehavioral disorders, leading to decreased locomotor activity and a reduction of the exploration behavior of the environment which reflects the establishment of a state of stress, anxiety, and depression. In fact, the histopathological analysis revealed significant lesions in the brain tissue. Furthermore, the administration of probiotics (Prob) allowed the rats to regain body and brain weight when compared to the intoxicated rats. In addition, the use of various behavioral test techniques reveals that the administration of Lactobacillus rhamnosus corrects the depressive state, locomotor hypoactivity, and anxiety observed in rats exposed to Pb. Blood antioxidant status analysis revealed that Prob restored the activity of different antioxidant enzymes (SOD, CAT, GPx, and GSH) and non-enzymatic enzymes (glutathione) in rats intoxicated and treated with Prob. In effect, the histological study showed a significant improvement in the brain histology in rats intoxicated and treated with Prob. This illustrates the important implications of probiotic lactobacilli and their therapeutic properties.

Phytotherapie, 2023

Huile essentielle et extraits bruts des parties aériennes de Pulicaria arabica (L.) Cass : compos... more Huile essentielle et extraits bruts des parties aériennes de Pulicaria arabica (L.) Cass : composition chimique, effet antioxydant, activités inhibitrices de l'α-glucosidase, de l'acétylcholinestérase et de la butyrylcholinestérase

The objective of this study, on the one hand, is to evaluate the impact of aluminium intoxication... more The objective of this study, on the one hand, is to evaluate the impact of aluminium intoxication, on the biochemical and hematological approaches of adult Wistar rats, on the other hand, to test the efficacy of Syzygium aromaticum essential oil (CEO) in restoring or not the harmful effects of the studied metal with a daily intraperitoneal injection of 0.1 ml CEO/kg. The extraction of the essential oil from S.aromaticum by hydro-distillation allowed us to obtain CEO with a yield of 10.18%. Chronic exposure to aluminum chloride at a concentration of 100mg/kg in rats decreased body and organ weights (Liver, Kidney) compared to controls. The intoxication also revealed a disruption of various biochemical parameters, including liver (TGO, TGP), and kidney (urea, creatinine) biochemical assays, Thus, the concentration of aluminium in the intoxicated group is high in the blood. Also, the results show a correction in the values of these parameters following the administration of CEO compared to those of exposed animals. CEO also corrected the shape of the affected blood cells after intoxication to aluminium.

Juniperus oxycedrus L. and Pistacia lentiscus L. are two taxa with a rigid or rigid enough struct... more Juniperus oxycedrus L. and Pistacia lentiscus L. are two taxa with a rigid or rigid enough structure caused by a solid pericarp resulting in a strong inhibition of the germination. The objective of this work was to test the effectiveness of certain pre-germinative treatments on the germination rate of the Juniperus oxycedrus and Pistacia lentiscus seeds with the aim of restoring perturbed ecosystems. In this context 180 seeds from each of the 2 examined species were tested in the laboratory (the number of repetitions is 5, to reach the degree of freedom 'ddl'). The seeds were divided into 4 groups of 20 seeds of each species and were treated as follows: 1) Immersion in the concentrated (95%) sulphuric acid (H 2 SO 4) for 10, 30 and 60 minutes; 2) Mechanical scarification with a scalpel; 3) Soaking in hot water (at 100°C) for 30 seconds, 60 seconds and 2 minutes; 4) Cold stratification at 5°C for 24 hours. There is also a control group of 20 seeds representing each of the two species, which remained neutral. In the case of the Juniperus oxycedrus seeds the results showed that the germination rate improves after the mechanical scarification with a scalpel (92%), followed by the cold stratification with water (86%) and a treatment with a concentrated sulphuric acid for 60 minutes (82%). The seeds were treated with H 2 SO 4 for 10 and 30 minutes, respectively, soaked in hot water; the control lot did not germinate. The germination rate for the Pistacia lentiscus seeds is 88% after the mechanical scarification and 84% after the cold water stratification. On the other hand, the treatment with H 2 SO 4 or after soaking in hot water does not allow for the germination of these seeds. The same result is observed on the control group (0% for 30 days).

The present study aimed to evaluate the protective effect of Cinnamomum cassia essential oil on n... more The present study aimed to evaluate the protective effect of Cinnamomum cassia essential oil on nephrotoxicity induced by manganese chloride (4.79mg/ml) in young Wistar rats during the development period. Wistar rat animals were exposed to manganese through maternal watering on a postnatal day (PND) 1 to (PND) 21. After weaning, the rats exposed to manganese received injections of Cinnamomum cassia essential oil (0.1 ml/kg) for 21 days. The concentration of manganese in the renal tissues was significantly increased compared to control rats. However, the levels of renal markers such as urea, uric acid, and creatinine were significantly increased in the blood after manganese chloride administration. Manganese-induced oxidative stress in renal tissue was indicated by decreased levels of superoxide dismutase, catalase, and glutathione peroxidase respectively. Histologically, the kidney showed several tissue alterations such as necrosis of the glomeruli and tubules. The administration of Cinnamomum cassia essential oil considerably attenuated the previous biochemical damage in serum and kidney tissue as well as histological and cellular modifications. In this study, it can be concluded that the Cinnamomum cassia essential oil showed a nephroprotective effect.

Purpose: Aluminum (Al) and its various forms are environmental xenobiotics that pose great threat... more Purpose: Aluminum (Al) and its various forms are environmental xenobiotics that pose great threats to public health. The probiotic strain has been reported to have important protective effects against aluminum cytotoxicity. Therefore, the current study was designed to assess the disruption of neurobehavioral and hematobiochemical parameters caused by chronic exposure to AlCl3, and then explore the protective effects of the probiotic strain Lactobacillus rhamnosus against these adverse in male rats. Method: Thirty-two animals were gathered into four groups: The first group served as control. The second group received 100mg AlCl3/kg bw for 12 weeks. However, the third group was administered 10mL probiotic strain /Kg (108 CFU/ ml) bw/d daily for 4 weeks. Finally, group 4 received AlCl3 for 12 weeks, then the treatment was continued with probiotic for 4 weeks. Results: AlCl3 produced a decrease in body and brain weights with respect to their control. The results obtained also showed significant depression and anxiety-like behavior in the intoxicated animals, with a disturbance of the tested hematobiochemical parameters, particularly in renal and hepatic biomarkers. Overall, probiotics prevented enduring AlCl3-induced depression-like behavior in intoxicated rats. The results of the hematobiochemical, renal and hepatic analyses show a correction of the values after the administration of probiotics compared to those of intoxicated and untreated animals. Conclusion: The probiotic lactobacilli would be an alternative to reduce AlCl3 toxicity.

Purpose: Mercury is one of the metallic trace elements. This metal is harmful to health. However,... more Purpose: Mercury is one of the metallic trace elements. This metal is harmful to health. However, Green anis is a plant widely used in the medical field for its various therapeutic virtues. The aim of this study is to evaluate the effect of subchonic exposure to mercury chloride (HgCl2) on the neurobehavioral status of young wistar rats as well as the neurocorrective effect of essential oil of green anis. Methods: Extraction of essential oil by hydrodisillation. Exposure of developing rats to a dose of (100mg/L). Then, a group of the intoxicated animals underwent therapy with one dose (0.25 ml/kg) for 21 days intraperitoneally. In addition, the realization of a behavior test battery namely; the forced swimming test to assess the state of despair of the pups, the open field to assess the locomotor activity as well as the exploratory capacities followed by a histopathological study of the brain. Data were analyzed by two-way analysis of variance (ANOVA). Results: The results of this study demonstrate that exposure to mercury induced significant decreases in body weight (p < 0.001) and brain weight (p < 0.05), respectively, increases the level of depression (p < 0.001), locomotor hypoactivity (p < 0.01) compared to control rats. Histological study revealed Purkinje cell degeneration, vascular congestion and lesions. Administration of Pimpinella anisum oil reduced levels of depression (p < 0.01), corrected locomotor hypoactivity (p < 0.05), and reduced mercury damage. Conclusion: According to this study, exposure to mercury disrupted the neurobehavioral state of young rats by affecting certain brain structures. These disturbances can be regulated by the essential oil of anis.

Phytotherapie, 2022

This work is a review of the results of some studies conducted around the world on the essential ... more This work is a review of the results of some studies conducted around the world on the essential oil of Mentha spicata . Thus, chemical analysis by gas chromatography-mass spectrometry (GC-MS) have identified several compounds in the oil. Among the main ones, we cited carvone, limonene, germacrene D, β-caryophyllene, menthone and 1,8-cineole. Indeed, the evaluation of the antioxidant activity of the essential oil by the DPPH test revealed a difference in the results that is probably due to the diversity of the chemical composition, factors and according to the region. As well as, the ABTS test results revealed that the essential oil of Mentha spicata possessed reasonable antioxidant activity. This could be attributed to the presence of a large amount of carvone. In addition, the evaluation of the antimicrobial activity of the essential oil by the technique of aromatogram and the determination of minimum inhibitory concentration (MIC) showed that it possessed a great inhibitory power on Gram positive bacteria like Staphylococcus aureus, Streptococcus pyogenes, Bacillus subtilis, Bacillus cereus , and Streptococcus mutans , and Gram negative bacteria like Pseudomonas aeruginosa, Pasturella multocida, Escherichia coli , and Salmonella. As well as on fungi such as Mucor mucedo, Rhizopus solani, Aspergillus niger, Aspergillus flavus , and Alternaria alternate . In another way, the results of cytotoxic activity using MTT assay showed that Mentha spicata essential oil had a potent cytotoxic action on KB and HeLa cells.

Bulletin of the University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Animal Science and Biotechnologies., 2021

The present study was carried out in order to evaluate, on the one hand, the modifications induce... more The present study was carried out in order to evaluate, on the one hand, the modifications induced by manganese chloride according to a neurobehavioral, biochemical and histological approach in developing Wistar rats and, on the other hand, to test the effectiveness of Foeniculum vulgare (fennel) essential oil (FEO) in restoring or not the harmful effects of manganese chloride (MnCl2). The characterization of this essential oil by gas chromatography showed that the major component is E-anethole (34.7907%). The administration of FEO corrected the depressive state, anxiety and locomotor hypoactivity respectively observed in rats exposed to MnCl2, thus, the FEO restored the activity of the various antioxidant enzymes with a clear improvement of the cerebral tissue architecture in intoxicated rats treated with FEO. In conclusion, the FEO has a beneficial effect on the nervous system of rats intoxicated with MnCl2 which justifies the importance of this oil in traditional medicine.

Phytotherapie, 2021

The principle aim of this work is to evaluate the antimicrobial and antiochratoxic power of the e... more The principle aim of this work is to evaluate the antimicrobial and antiochratoxic power of the essential oil of Mentha spicata (EOM). This oil was obtained by hydrodistillation with a yield of 0.3%. EOM was screened for its possible antibacterial activity in vitro against five strains of pathogenic bacteria, using the solid disc diffusion method and the microdilution method. The extract reacted positively to the bacterial strains tested. The results of the antifungal activity show an inhibition of mycelial growth; it is total on Aspergillus ochraceus, Aspergillus flavus, Aspergillus niger, Penicillium viridicatum, and on Rhizopus stolonifer and Alternaria alternate at a concentration of 12.5 μl/ml. Tests carried out on yeasts mainly referenced as Candida albicans IP444 and Candida albicans 2679 showed minimal inhibition (MIC) of the order of 7.81 μl/ml and 0.97 μl/ml, respectively and the MIC is of the order of 0.48 μl/ml for Candida albicans 10 231. In addition, the EOM antiochratoxigenic test for ochratoxin A production capacity following a series of high performance liquid chromatography separation and ultraviolet detection (HPLC-UV) analyses detected an inhibitory effect proportional to the EOM concentration. In general, the essential oil showed an effective antimicrobial action on the growth of the tested pathogens and an antiochratoxigenic.

Bulletin of University of Agricultural Sciences and Veterinary Medicin Cluj-Napoca. Veterinary Medicine, 2021

The objective of this study, on the one hand, is to evaluate the impact of Candida Albicans infec... more The objective of this study, on the one hand, is to evaluate the impact of Candida Albicans infection (10 6 CFU per 0.2 ml of intravenous inoculum) on the biochemical and hematological approaches of adult Wistar rats, on the other hand, to test the efficacy of Syzygium aromaticum essential oil (CEO) in restoring or not the harmful effects of the studied yeast with a daily intraperitoneal injection of 0.1 ml CEO/kg. The extraction of the essential oil from S.aromaticum by hydro-distillation allowed us to obtain CEO with a yield of 10.18%. Infection of adult rats with Candida albicans by intravenous injection at a concentration of 10 6 CFU per 0.2 ml of inoculum increased hepatic and renal fungal load. The infection also revealed a disruption of various biochemical parameters, including liver (AST, ALT), kidney (urea, creatinine) biochemical assays, and hematological (white and red blood cells, platelets...); also, the results show a correction in the values of these parameters following the administration of CEO (by an intraperitoneal injection of 0.1 ml CEO/kg/day) compared to those of infected animals. CEO also corrected the shape of the affected blood cells after infection with C. albicans.

Notulae Scientia Biologicae, 2021

The purpose of this study was to evaluate the prophylactic effect of Pimpinella anisum (green ani... more The purpose of this study was to evaluate the prophylactic effect of Pimpinella anisum (green anis) on neurobehavioral status following mercury chloride intoxication during the developmental period. For this purpose, rats exposed to 100 mg/L of HgCl2 during the gestation and lactation period. A group of rats was treated with the anis extract for 15 days before becoming intoxicated with mercury. In contrast, one group was orally administered aqueous anis extract for 15 days after intoxication. The forced swimming test, the open field test and the Morris pool respectively recorded an increase in immobility time, a decrease in the number of cross-cells (p <0.001), (p <0.05) and an increase in latency (p <0.01), (p <0.001), (p <0.001) and decreased time spent in the target frame during the probe test (p <0.01) and increased latency in the visible test (p <0.01) in HgCl2-exposed rats compared to control rats. However, preventive and curative aniseed-based treatment reduced the rate of depression, increased locomotor activity and improved learning performance. In conclusion, the aqueous extract of Pimpinella anisum could have a corrective effect on some neurological disorders caused by mercury.

Notulae Scientia Biologicae, 2020

Our work focused on the impact of chronic lead acetate and manganese chloride poisoning on renal ... more Our work focused on the impact of chronic lead acetate and manganese chloride poisoning on renal function in Wistar rats. It also tested the efficacy of the essential oil extracted from (Mentha spicata) spearmint in reversing these effects by treating previously poisoned rats with a daily intraperitoneal injection of 0.1 ml HEM/kg over a 21-days period. Analysis of this essential oil through gas chromatography coupled with mass spectrometry revealed that the main components are: carvone (42.2%), menthone (20.89%), piperitenone (17.41%) and isomenthone (7.99%). Chronic oral co-exposure to lead and manganese during periods of gestation and lactation resulted in a significant reduction (P<0.01) in both body and kidney weight. Further examination of renal function revealed significant disturbances in the quantity of markers of renal function (creatinine, urea, uric acid) in the blood. Chronic co-exposure led to the observation of disruption to antioxidant enzyme (superoxide dismutase, glutathione peroxidase and catalase) activity in poisoned rats in comparison with the control rats; these results suggest renal failure. Indeed, histological study of the kidneys revealed pronounced damage manifesting as cell degeneration with the renal parenchyma affected by inflammation. In addition, the administration of the essential oil of Mentha spicata led to the observation of an increase in body weight, regulation of the various biochemical markers, and the regeneration of damaged renal tissue, all of which proves the high importance of spearmint in traditional medicine, and its therapeutic properties in combating renal problems.

Journal of Drug Delivery and Therapeutics (JDDT), 2020

The present study was designed to evaluate the protective effect of Cinnamomum cassia essential o... more The present study was designed to evaluate the protective effect of Cinnamomum cassia essential oil (CCEO) on hepatotoxicity induced by co-exposure to lead acetate (Pb) (2.84mg/ml) and manganese chloride (Mn) (4.79mg/ml) in developing Wistar rats. After we aning, rats exposed to Pb-Mn received injections of Cinnamomum cassia essential oil (0.1 ml/kg) for 21 days. Extraction by hydrodistillation yielded 1.10% of OECC and the characterization of this oil by GC-MS indicates that the major components of this oil are: E-cinnamaldehyde (85.77%), linalool (3.70%), Z-cinnamaldehyde (3.22%) and B-phellandréne (1.19%). The concentration of lead and manganese in hepatocyte tissues was significantly increased compared to control rats. However, levels of hepatic markers such as Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), alkaline phosphatase (ALP) were significantly increased in blood in Pb-Mn poisoned rats. Pb-Mn-induced oxidative stress in liver tissue was indicated by decreased levels of superoxide dismutase, catalase and glutathione peroxidase respectively. Histologically, the liver showed several tissue alterations such as hepatocyte necrosis and the presence of steatosis foci. Administration of OECC considerably attenuated previous biochemical alterations as well as histological and cellular changes in liver tissue. In this study, we can conclude that the Cinnamomum cassia essential oil showed a hepatoprotective effect. The therapeutic effect of Cinnamomum cassia essential oil against hepatotoxicity induced by co-exposure to lead and manganese in developing Wistar rats, Journal of Drug Delivery and Therapeutics. 2020; 10(5):49-55

Journal of Applied and Natural Science, 2020

Aluminum is present in medicines and food. Its toxicity induces deleterious effects in various l... more Aluminum is present in medicines and food. Its toxicity induces deleterious effects in various living organisms. At the same time, Hordeum vulgare a cereal known as an important nutritional source and also endowed with bioactive molecules. The objective of this study was to evaluate, on the one hand, the modifications induced by aluminum chloride in Wistar rats at the cerebral level and, on the other hand, to test the efficacy of the barley extract, Hordeum vulgare, (HEV) to restore the harmful effects of this studied metal with a concentration of 13 ml HEV/kg/day for a period of 21 days. The extraction of HEV by maceration resulted in an aqueous extract with a yield of 10.70%. Exposure to AlCl3 at a concentration of 100mg/kg, permitted to observe that the concentration of aluminium at the brain level is significantly high (p<0.05) in the intoxicated rats compared to the control rats. On the other hand, the activity of alkaline phosphatase (PAL), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) indicated a decrease in the intoxicated rats. Indeed, the histological study showed very pronounced lesions in the brains of the poisoned rats resulting in necrosis and cellular spongiosis. In addition, the administration of HEV restored the activity of the various antioxidant enzymes with an improvement in brain tissue architecture in intoxicated rats treated with HEV which justifies the therapeutic virtues of H. vulgare in protecting against aluminium chloride-induced neurotoxicity.

Notulae Scientia Biologicae, 2020

The mechanisms that cause nickel (Ni) toxicity are multiple and potentially affect all cells in t... more The mechanisms that cause nickel (Ni) toxicity are multiple and potentially affect all cells in the body. For this purpose, we were interested in the oxidative stress induced by the Ni at the erythrocyte level at a dose of 2 g/l during the gestation and lactation period in the Wistar rats and the capacity of the clove essential oil, Syzygium aromaticum, (CEO) to restore or not this state of stress. Extraction of the CEO by hydro-distillation allowed us to obtain a CEO with a yield of 10.60% and the characterization of this essential oil by GC-MS indicates that the major components of this oil are: Eugenol (80.95%), eugenyl acetate (10.48%), β-caryophyllene (7.21%) and α-humulene (0.87%). The antioxidant activity of the CEO made in vitro showed a free radical scavenging capacity DPPH with an IC 50 of the order of 25.60 μg/ml. In addition, analysis of the erythrocyte antioxidant status indicated that Ni significantly increased the enzymatic activities of catalase (CAT) and superoxide dismutase (SOD) and significantly decreased the enzymatic activity of glutathione peroxidase (GPx), resulting in dysfunction of the antioxidant defense system. On the other hand, the administration of CEO by intraperitoneal (IP) over a period of 21 days to rats previously intoxicated with Ni, indicates that this CEO contributes significantly in improving defenses against free radical aggression, through a recovery. At the level of antioxidant enzyme activities by increasing their abilities to eliminate radical compounds.

INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY, 2020

This study evaluated the antioxidant activity of Mentha Spicata essential oil against to lead and... more This study evaluated the antioxidant activity of Mentha Spicata essential oil against to lead and manganese-induced brain oxidative damage in Wistar rats. Chronic oral co-exposure to lead (0.2%) and manganese (4.79 mg/ml) during periods of gestation and lactation resulted in a significant reduction in both body and brain weight. produced significantly reduced in superoxide dismutase (SOD) content, and decreased catalase (CAT), and glutathionperoxydase (GPx) activities were observed in the braintissues; however, the administration of the essential oil of Mentha spicata with intraperitoneal injection of 0.1 ml HEM/kg over a 21-days periodsignificantly improved the various in the both body and brain weight. These results indicate that Mentha spicata has an antioxidant effect against lead and manganeseinduced brain oxidative damage and is useful as a neuroprotective agent against various brain diseases induced by oxidative stress

Notulae Scientia Biologicae, 2021

The purpose of this study was to evaluate the prophylactic effect of Pimpinella anisum (green ani... more The purpose of this study was to evaluate the prophylactic effect of Pimpinella anisum (green anis) on neurobehavioral status following mercury chloride intoxication during the developmental period. For this purpose, rats exposed to 100 mg/L of HgCl2 during the gestation and lactation period. A group of rats was treated with the anis extract for 15 days before becoming intoxicated with mercury. In contrast, one group was orally administered aqueous anis extract for 15 days after intoxication. The forced swimming test, the open field test and the Morris pool respectively recorded an increase in immobility time, a decrease in the number of cross-cells (p <0.001), (p <0.05) and an increase in latency (p <0.01), (p <0.001), (p <0.001) and decreased time spent in the target frame during the probe test (p <0.01) and increased latency in the visible test (p <0.01) in HgCl2 - exposed rats compared to control rats. However, preventive and curative aniseed-based treatment...

Natural Product Research, 2024

Plants have gained great importance. Secondary metabolites contribute to the drug discovery and d... more Plants have gained great importance. Secondary metabolites contribute to the drug discovery and development by their bioactive properties. Rubia tinctorum L. essential oil (EO) was obtained and analysed. Antioxidant and antibacterial activities were evaluated. The plant's EOs were obtained through steam distillation, and the compounds were identified using gas chromatography-mass spectrometry (GC-MS) analysis. DPPH free radical scavenging and ferric-reducing antioxidant power (FRAP) were employed to assess antioxidant activity. Total antioxidant capacity (TAC) was also presented. The disc diffusion method was employed for testing antibacterial activity. Cyclohexanone was identified as the predominant component in the EO, constituting 88.74% of the total composition. The EO did not show significant antioxidant capacity, while it demonstrated antimicrobial effect against Bacillus cereus ATCC 6633 (>13 mm of inhibition; 500 mg/mL) and Shigella ATCC 12022 (≥12 mm of inhibition; 500 mg/mL). R. tinctorum L. is new source of cyclohexanone.

Fresenius Environmental Bulletin , 2023

This study aims to examine the effects induced by lead acetate (Pb) using different experimental ... more This study aims to examine the effects induced by lead acetate (Pb) using different experimental approaches, such as neurobehavioral, biochemical, and histological in young Wistar rats during gestation and lactation, and to test the probiotic effect of Lactobacillus rhamnosus (Prob) throughout correcting or not the harmful effects of the studied metal. Chronic exposure to 0.2% lead acetate in rats during the development period resulted in a decrease in body and brain weights compared to controls. Test results for depression (forced swimming test), anxiety (Elevated plus maze), and locomotor and explo ratory activity (Open field) all show that Pb intoxication causes neurobehavioral disorders, leading to decreased locomotor activity and a reduction of the exploration behavior of the environment which reflects the establishment of a state of stress, anxiety, and depression. In fact, the histopathological analysis revealed significant lesions in the brain tissue. Furthermore, the administration of probiotics (Prob) allowed the rats to regain body and brain weight when compared to the intoxicated rats. In addition, the use of various behavioral test techniques reveals that the administration of Lactobacillus rhamnosus corrects the depressive state, locomotor hypoactivity, and anxiety observed in rats exposed to Pb. Blood antioxidant status analysis revealed that Prob restored the activity of different antioxidant enzymes (SOD, CAT, GPx, and GSH) and non-enzymatic enzymes (glutathione) in rats intoxicated and treated with Prob. In effect, the histological study showed a significant improvement in the brain histology in rats intoxicated and treated with Prob. This illustrates the important implications of probiotic lactobacilli and their therapeutic properties.

Phytotherapie, 2023

Huile essentielle et extraits bruts des parties aériennes de Pulicaria arabica (L.) Cass : compos... more Huile essentielle et extraits bruts des parties aériennes de Pulicaria arabica (L.) Cass : composition chimique, effet antioxydant, activités inhibitrices de l'α-glucosidase, de l'acétylcholinestérase et de la butyrylcholinestérase

The objective of this study, on the one hand, is to evaluate the impact of aluminium intoxication... more The objective of this study, on the one hand, is to evaluate the impact of aluminium intoxication, on the biochemical and hematological approaches of adult Wistar rats, on the other hand, to test the efficacy of Syzygium aromaticum essential oil (CEO) in restoring or not the harmful effects of the studied metal with a daily intraperitoneal injection of 0.1 ml CEO/kg. The extraction of the essential oil from S.aromaticum by hydro-distillation allowed us to obtain CEO with a yield of 10.18%. Chronic exposure to aluminum chloride at a concentration of 100mg/kg in rats decreased body and organ weights (Liver, Kidney) compared to controls. The intoxication also revealed a disruption of various biochemical parameters, including liver (TGO, TGP), and kidney (urea, creatinine) biochemical assays, Thus, the concentration of aluminium in the intoxicated group is high in the blood. Also, the results show a correction in the values of these parameters following the administration of CEO compared to those of exposed animals. CEO also corrected the shape of the affected blood cells after intoxication to aluminium.

Juniperus oxycedrus L. and Pistacia lentiscus L. are two taxa with a rigid or rigid enough struct... more Juniperus oxycedrus L. and Pistacia lentiscus L. are two taxa with a rigid or rigid enough structure caused by a solid pericarp resulting in a strong inhibition of the germination. The objective of this work was to test the effectiveness of certain pre-germinative treatments on the germination rate of the Juniperus oxycedrus and Pistacia lentiscus seeds with the aim of restoring perturbed ecosystems. In this context 180 seeds from each of the 2 examined species were tested in the laboratory (the number of repetitions is 5, to reach the degree of freedom 'ddl'). The seeds were divided into 4 groups of 20 seeds of each species and were treated as follows: 1) Immersion in the concentrated (95%) sulphuric acid (H 2 SO 4) for 10, 30 and 60 minutes; 2) Mechanical scarification with a scalpel; 3) Soaking in hot water (at 100°C) for 30 seconds, 60 seconds and 2 minutes; 4) Cold stratification at 5°C for 24 hours. There is also a control group of 20 seeds representing each of the two species, which remained neutral. In the case of the Juniperus oxycedrus seeds the results showed that the germination rate improves after the mechanical scarification with a scalpel (92%), followed by the cold stratification with water (86%) and a treatment with a concentrated sulphuric acid for 60 minutes (82%). The seeds were treated with H 2 SO 4 for 10 and 30 minutes, respectively, soaked in hot water; the control lot did not germinate. The germination rate for the Pistacia lentiscus seeds is 88% after the mechanical scarification and 84% after the cold water stratification. On the other hand, the treatment with H 2 SO 4 or after soaking in hot water does not allow for the germination of these seeds. The same result is observed on the control group (0% for 30 days).

The present study aimed to evaluate the protective effect of Cinnamomum cassia essential oil on n... more The present study aimed to evaluate the protective effect of Cinnamomum cassia essential oil on nephrotoxicity induced by manganese chloride (4.79mg/ml) in young Wistar rats during the development period. Wistar rat animals were exposed to manganese through maternal watering on a postnatal day (PND) 1 to (PND) 21. After weaning, the rats exposed to manganese received injections of Cinnamomum cassia essential oil (0.1 ml/kg) for 21 days. The concentration of manganese in the renal tissues was significantly increased compared to control rats. However, the levels of renal markers such as urea, uric acid, and creatinine were significantly increased in the blood after manganese chloride administration. Manganese-induced oxidative stress in renal tissue was indicated by decreased levels of superoxide dismutase, catalase, and glutathione peroxidase respectively. Histologically, the kidney showed several tissue alterations such as necrosis of the glomeruli and tubules. The administration of Cinnamomum cassia essential oil considerably attenuated the previous biochemical damage in serum and kidney tissue as well as histological and cellular modifications. In this study, it can be concluded that the Cinnamomum cassia essential oil showed a nephroprotective effect.

Purpose: Aluminum (Al) and its various forms are environmental xenobiotics that pose great threat... more Purpose: Aluminum (Al) and its various forms are environmental xenobiotics that pose great threats to public health. The probiotic strain has been reported to have important protective effects against aluminum cytotoxicity. Therefore, the current study was designed to assess the disruption of neurobehavioral and hematobiochemical parameters caused by chronic exposure to AlCl3, and then explore the protective effects of the probiotic strain Lactobacillus rhamnosus against these adverse in male rats. Method: Thirty-two animals were gathered into four groups: The first group served as control. The second group received 100mg AlCl3/kg bw for 12 weeks. However, the third group was administered 10mL probiotic strain /Kg (108 CFU/ ml) bw/d daily for 4 weeks. Finally, group 4 received AlCl3 for 12 weeks, then the treatment was continued with probiotic for 4 weeks. Results: AlCl3 produced a decrease in body and brain weights with respect to their control. The results obtained also showed significant depression and anxiety-like behavior in the intoxicated animals, with a disturbance of the tested hematobiochemical parameters, particularly in renal and hepatic biomarkers. Overall, probiotics prevented enduring AlCl3-induced depression-like behavior in intoxicated rats. The results of the hematobiochemical, renal and hepatic analyses show a correction of the values after the administration of probiotics compared to those of intoxicated and untreated animals. Conclusion: The probiotic lactobacilli would be an alternative to reduce AlCl3 toxicity.

Purpose: Mercury is one of the metallic trace elements. This metal is harmful to health. However,... more Purpose: Mercury is one of the metallic trace elements. This metal is harmful to health. However, Green anis is a plant widely used in the medical field for its various therapeutic virtues. The aim of this study is to evaluate the effect of subchonic exposure to mercury chloride (HgCl2) on the neurobehavioral status of young wistar rats as well as the neurocorrective effect of essential oil of green anis. Methods: Extraction of essential oil by hydrodisillation. Exposure of developing rats to a dose of (100mg/L). Then, a group of the intoxicated animals underwent therapy with one dose (0.25 ml/kg) for 21 days intraperitoneally. In addition, the realization of a behavior test battery namely; the forced swimming test to assess the state of despair of the pups, the open field to assess the locomotor activity as well as the exploratory capacities followed by a histopathological study of the brain. Data were analyzed by two-way analysis of variance (ANOVA). Results: The results of this study demonstrate that exposure to mercury induced significant decreases in body weight (p < 0.001) and brain weight (p < 0.05), respectively, increases the level of depression (p < 0.001), locomotor hypoactivity (p < 0.01) compared to control rats. Histological study revealed Purkinje cell degeneration, vascular congestion and lesions. Administration of Pimpinella anisum oil reduced levels of depression (p < 0.01), corrected locomotor hypoactivity (p < 0.05), and reduced mercury damage. Conclusion: According to this study, exposure to mercury disrupted the neurobehavioral state of young rats by affecting certain brain structures. These disturbances can be regulated by the essential oil of anis.

Phytotherapie, 2022

This work is a review of the results of some studies conducted around the world on the essential ... more This work is a review of the results of some studies conducted around the world on the essential oil of Mentha spicata . Thus, chemical analysis by gas chromatography-mass spectrometry (GC-MS) have identified several compounds in the oil. Among the main ones, we cited carvone, limonene, germacrene D, β-caryophyllene, menthone and 1,8-cineole. Indeed, the evaluation of the antioxidant activity of the essential oil by the DPPH test revealed a difference in the results that is probably due to the diversity of the chemical composition, factors and according to the region. As well as, the ABTS test results revealed that the essential oil of Mentha spicata possessed reasonable antioxidant activity. This could be attributed to the presence of a large amount of carvone. In addition, the evaluation of the antimicrobial activity of the essential oil by the technique of aromatogram and the determination of minimum inhibitory concentration (MIC) showed that it possessed a great inhibitory power on Gram positive bacteria like Staphylococcus aureus, Streptococcus pyogenes, Bacillus subtilis, Bacillus cereus , and Streptococcus mutans , and Gram negative bacteria like Pseudomonas aeruginosa, Pasturella multocida, Escherichia coli , and Salmonella. As well as on fungi such as Mucor mucedo, Rhizopus solani, Aspergillus niger, Aspergillus flavus , and Alternaria alternate . In another way, the results of cytotoxic activity using MTT assay showed that Mentha spicata essential oil had a potent cytotoxic action on KB and HeLa cells.

Bulletin of the University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Animal Science and Biotechnologies., 2021

The present study was carried out in order to evaluate, on the one hand, the modifications induce... more The present study was carried out in order to evaluate, on the one hand, the modifications induced by manganese chloride according to a neurobehavioral, biochemical and histological approach in developing Wistar rats and, on the other hand, to test the effectiveness of Foeniculum vulgare (fennel) essential oil (FEO) in restoring or not the harmful effects of manganese chloride (MnCl2). The characterization of this essential oil by gas chromatography showed that the major component is E-anethole (34.7907%). The administration of FEO corrected the depressive state, anxiety and locomotor hypoactivity respectively observed in rats exposed to MnCl2, thus, the FEO restored the activity of the various antioxidant enzymes with a clear improvement of the cerebral tissue architecture in intoxicated rats treated with FEO. In conclusion, the FEO has a beneficial effect on the nervous system of rats intoxicated with MnCl2 which justifies the importance of this oil in traditional medicine.

Phytotherapie, 2021

The principle aim of this work is to evaluate the antimicrobial and antiochratoxic power of the e... more The principle aim of this work is to evaluate the antimicrobial and antiochratoxic power of the essential oil of Mentha spicata (EOM). This oil was obtained by hydrodistillation with a yield of 0.3%. EOM was screened for its possible antibacterial activity in vitro against five strains of pathogenic bacteria, using the solid disc diffusion method and the microdilution method. The extract reacted positively to the bacterial strains tested. The results of the antifungal activity show an inhibition of mycelial growth; it is total on Aspergillus ochraceus, Aspergillus flavus, Aspergillus niger, Penicillium viridicatum, and on Rhizopus stolonifer and Alternaria alternate at a concentration of 12.5 μl/ml. Tests carried out on yeasts mainly referenced as Candida albicans IP444 and Candida albicans 2679 showed minimal inhibition (MIC) of the order of 7.81 μl/ml and 0.97 μl/ml, respectively and the MIC is of the order of 0.48 μl/ml for Candida albicans 10 231. In addition, the EOM antiochratoxigenic test for ochratoxin A production capacity following a series of high performance liquid chromatography separation and ultraviolet detection (HPLC-UV) analyses detected an inhibitory effect proportional to the EOM concentration. In general, the essential oil showed an effective antimicrobial action on the growth of the tested pathogens and an antiochratoxigenic.

Bulletin of University of Agricultural Sciences and Veterinary Medicin Cluj-Napoca. Veterinary Medicine, 2021

The objective of this study, on the one hand, is to evaluate the impact of Candida Albicans infec... more The objective of this study, on the one hand, is to evaluate the impact of Candida Albicans infection (10 6 CFU per 0.2 ml of intravenous inoculum) on the biochemical and hematological approaches of adult Wistar rats, on the other hand, to test the efficacy of Syzygium aromaticum essential oil (CEO) in restoring or not the harmful effects of the studied yeast with a daily intraperitoneal injection of 0.1 ml CEO/kg. The extraction of the essential oil from S.aromaticum by hydro-distillation allowed us to obtain CEO with a yield of 10.18%. Infection of adult rats with Candida albicans by intravenous injection at a concentration of 10 6 CFU per 0.2 ml of inoculum increased hepatic and renal fungal load. The infection also revealed a disruption of various biochemical parameters, including liver (AST, ALT), kidney (urea, creatinine) biochemical assays, and hematological (white and red blood cells, platelets...); also, the results show a correction in the values of these parameters following the administration of CEO (by an intraperitoneal injection of 0.1 ml CEO/kg/day) compared to those of infected animals. CEO also corrected the shape of the affected blood cells after infection with C. albicans.

Notulae Scientia Biologicae, 2021

The purpose of this study was to evaluate the prophylactic effect of Pimpinella anisum (green ani... more The purpose of this study was to evaluate the prophylactic effect of Pimpinella anisum (green anis) on neurobehavioral status following mercury chloride intoxication during the developmental period. For this purpose, rats exposed to 100 mg/L of HgCl2 during the gestation and lactation period. A group of rats was treated with the anis extract for 15 days before becoming intoxicated with mercury. In contrast, one group was orally administered aqueous anis extract for 15 days after intoxication. The forced swimming test, the open field test and the Morris pool respectively recorded an increase in immobility time, a decrease in the number of cross-cells (p <0.001), (p <0.05) and an increase in latency (p <0.01), (p <0.001), (p <0.001) and decreased time spent in the target frame during the probe test (p <0.01) and increased latency in the visible test (p <0.01) in HgCl2-exposed rats compared to control rats. However, preventive and curative aniseed-based treatment reduced the rate of depression, increased locomotor activity and improved learning performance. In conclusion, the aqueous extract of Pimpinella anisum could have a corrective effect on some neurological disorders caused by mercury.

Notulae Scientia Biologicae, 2020

Our work focused on the impact of chronic lead acetate and manganese chloride poisoning on renal ... more Our work focused on the impact of chronic lead acetate and manganese chloride poisoning on renal function in Wistar rats. It also tested the efficacy of the essential oil extracted from (Mentha spicata) spearmint in reversing these effects by treating previously poisoned rats with a daily intraperitoneal injection of 0.1 ml HEM/kg over a 21-days period. Analysis of this essential oil through gas chromatography coupled with mass spectrometry revealed that the main components are: carvone (42.2%), menthone (20.89%), piperitenone (17.41%) and isomenthone (7.99%). Chronic oral co-exposure to lead and manganese during periods of gestation and lactation resulted in a significant reduction (P<0.01) in both body and kidney weight. Further examination of renal function revealed significant disturbances in the quantity of markers of renal function (creatinine, urea, uric acid) in the blood. Chronic co-exposure led to the observation of disruption to antioxidant enzyme (superoxide dismutase, glutathione peroxidase and catalase) activity in poisoned rats in comparison with the control rats; these results suggest renal failure. Indeed, histological study of the kidneys revealed pronounced damage manifesting as cell degeneration with the renal parenchyma affected by inflammation. In addition, the administration of the essential oil of Mentha spicata led to the observation of an increase in body weight, regulation of the various biochemical markers, and the regeneration of damaged renal tissue, all of which proves the high importance of spearmint in traditional medicine, and its therapeutic properties in combating renal problems.

Journal of Drug Delivery and Therapeutics (JDDT), 2020

The present study was designed to evaluate the protective effect of Cinnamomum cassia essential o... more The present study was designed to evaluate the protective effect of Cinnamomum cassia essential oil (CCEO) on hepatotoxicity induced by co-exposure to lead acetate (Pb) (2.84mg/ml) and manganese chloride (Mn) (4.79mg/ml) in developing Wistar rats. After we aning, rats exposed to Pb-Mn received injections of Cinnamomum cassia essential oil (0.1 ml/kg) for 21 days. Extraction by hydrodistillation yielded 1.10% of OECC and the characterization of this oil by GC-MS indicates that the major components of this oil are: E-cinnamaldehyde (85.77%), linalool (3.70%), Z-cinnamaldehyde (3.22%) and B-phellandréne (1.19%). The concentration of lead and manganese in hepatocyte tissues was significantly increased compared to control rats. However, levels of hepatic markers such as Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), alkaline phosphatase (ALP) were significantly increased in blood in Pb-Mn poisoned rats. Pb-Mn-induced oxidative stress in liver tissue was indicated by decreased levels of superoxide dismutase, catalase and glutathione peroxidase respectively. Histologically, the liver showed several tissue alterations such as hepatocyte necrosis and the presence of steatosis foci. Administration of OECC considerably attenuated previous biochemical alterations as well as histological and cellular changes in liver tissue. In this study, we can conclude that the Cinnamomum cassia essential oil showed a hepatoprotective effect. The therapeutic effect of Cinnamomum cassia essential oil against hepatotoxicity induced by co-exposure to lead and manganese in developing Wistar rats, Journal of Drug Delivery and Therapeutics. 2020; 10(5):49-55

Journal of Applied and Natural Science, 2020

Aluminum is present in medicines and food. Its toxicity induces deleterious effects in various l... more Aluminum is present in medicines and food. Its toxicity induces deleterious effects in various living organisms. At the same time, Hordeum vulgare a cereal known as an important nutritional source and also endowed with bioactive molecules. The objective of this study was to evaluate, on the one hand, the modifications induced by aluminum chloride in Wistar rats at the cerebral level and, on the other hand, to test the efficacy of the barley extract, Hordeum vulgare, (HEV) to restore the harmful effects of this studied metal with a concentration of 13 ml HEV/kg/day for a period of 21 days. The extraction of HEV by maceration resulted in an aqueous extract with a yield of 10.70%. Exposure to AlCl3 at a concentration of 100mg/kg, permitted to observe that the concentration of aluminium at the brain level is significantly high (p<0.05) in the intoxicated rats compared to the control rats. On the other hand, the activity of alkaline phosphatase (PAL), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) indicated a decrease in the intoxicated rats. Indeed, the histological study showed very pronounced lesions in the brains of the poisoned rats resulting in necrosis and cellular spongiosis. In addition, the administration of HEV restored the activity of the various antioxidant enzymes with an improvement in brain tissue architecture in intoxicated rats treated with HEV which justifies the therapeutic virtues of H. vulgare in protecting against aluminium chloride-induced neurotoxicity.

Notulae Scientia Biologicae, 2020

The mechanisms that cause nickel (Ni) toxicity are multiple and potentially affect all cells in t... more The mechanisms that cause nickel (Ni) toxicity are multiple and potentially affect all cells in the body. For this purpose, we were interested in the oxidative stress induced by the Ni at the erythrocyte level at a dose of 2 g/l during the gestation and lactation period in the Wistar rats and the capacity of the clove essential oil, Syzygium aromaticum, (CEO) to restore or not this state of stress. Extraction of the CEO by hydro-distillation allowed us to obtain a CEO with a yield of 10.60% and the characterization of this essential oil by GC-MS indicates that the major components of this oil are: Eugenol (80.95%), eugenyl acetate (10.48%), β-caryophyllene (7.21%) and α-humulene (0.87%). The antioxidant activity of the CEO made in vitro showed a free radical scavenging capacity DPPH with an IC 50 of the order of 25.60 μg/ml. In addition, analysis of the erythrocyte antioxidant status indicated that Ni significantly increased the enzymatic activities of catalase (CAT) and superoxide dismutase (SOD) and significantly decreased the enzymatic activity of glutathione peroxidase (GPx), resulting in dysfunction of the antioxidant defense system. On the other hand, the administration of CEO by intraperitoneal (IP) over a period of 21 days to rats previously intoxicated with Ni, indicates that this CEO contributes significantly in improving defenses against free radical aggression, through a recovery. At the level of antioxidant enzyme activities by increasing their abilities to eliminate radical compounds.

INTERNATIONAL JOURNAL OF RESEARCH IN PHARMACY AND CHEMISTRY, 2020

This study evaluated the antioxidant activity of Mentha Spicata essential oil against to lead and... more This study evaluated the antioxidant activity of Mentha Spicata essential oil against to lead and manganese-induced brain oxidative damage in Wistar rats. Chronic oral co-exposure to lead (0.2%) and manganese (4.79 mg/ml) during periods of gestation and lactation resulted in a significant reduction in both body and brain weight. produced significantly reduced in superoxide dismutase (SOD) content, and decreased catalase (CAT), and glutathionperoxydase (GPx) activities were observed in the braintissues; however, the administration of the essential oil of Mentha spicata with intraperitoneal injection of 0.1 ml HEM/kg over a 21-days periodsignificantly improved the various in the both body and brain weight. These results indicate that Mentha spicata has an antioxidant effect against lead and manganeseinduced brain oxidative damage and is useful as a neuroprotective agent against various brain diseases induced by oxidative stress

Bulletin of University of Agricultural Sciences and Veterinary Medicin Cluj-Napoca. Veterinary Medicine, 2021

The objective of this study, on the one hand, is to evaluate the impact of Candida Albicans infec... more The objective of this study, on the one hand, is to evaluate the impact of Candida Albicans infection (10 6 CFU per 0.2 ml of intravenous inoculum) on the biochemical and hematological approaches of adult Wistar rats, on the other hand, to test the efficacy of Syzygium aromaticum essential oil (CEO) in restoring or not the harmful effects of the studied yeast with a daily intraperitoneal injection of 0.1 ml CEO/kg. The extraction of the essential oil from S.aromaticum by hydro-distillation allowed us to obtain CEO with a yield of 10.18%. Infection of adult rats with Candida albicans by intravenous injection at a concentration of 10 6 CFU per 0.2 ml of inoculum increased hepatic and renal fungal load. The infection also revealed a disruption of various biochemical parameters, including liver (AST, ALT), kidney (urea, creatinine) biochemical assays, and hematological (white and red blood cells, platelets...); also, the results show a correction in the values of these parameters following the administration of CEO (by an intraperitoneal injection of 0.1 ml CEO/kg/day) compared to those of infected animals. CEO also corrected the shape of the affected blood cells after infection with C. albicans.