Cansu Önlen Güneri | Cukurova University (original) (raw)

Papers by Cansu Önlen Güneri

Kafkas üniversitesi veteriner fakültesi dergisi/Kafkas üniversitesi veteriner fakültesi dergisi, 2024

This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International Lic... more This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0)

DergiPark (Istanbul University), May 15, 2023

With this study, it was aimed to determine the prevalence of Leishmania species in cats in Hatay ... more With this study, it was aimed to determine the prevalence of Leishmania species in cats in Hatay province and also to illuminate relationship between some clinical findings and the disease. In the research, 100 cats were examined, which brought to Hatay Metropolitan Municipality Stray Animal Care and Rehabilitation Center and Hatay Mustafa Kemal University Veterinary Health, Practice and Research Hospital from different regions of Hatay province for diagnosis. Blood samples were taken from all animals. In addition, lymphatic fluid was taken from those who were found to have lymph enlargement. Smears were drawn from blood samples, inoculated on Roswell Park Memorial Institute 1640 medium, and subjected to PCR analyses. Of the cats sampled in the study, 59 were female, while 41 were male. The mean age (X) and standard deviation (SD) of the cats were found to be 3.32 ± 1.5, respectively (min: 0.5; max: 7). As a result of the analysis of the samples obtained from 100 cats examined of different ages and genders with different diagnostic methods, Leishmania positivity was not detected in cats in Hatay province. Although gender was not associated with the appearance of clinical findings (P> 0.05), clinical findings in cats increased significantly with increasing their age (P < 0.01).

Zenodo (CERN European Organization for Nuclear Research), Jan 29, 2023

The aim of this study was to determine the occurrence of Acinetobacter spp. in tank milk samples ... more The aim of this study was to determine the occurrence of Acinetobacter spp. in tank milk samples (TMS) in Hatay province and to analyze their antimicrobial susceptibility profile. In this study, a total of 60 TMS were collected between April and December 2016. Acinetobacter spp. was identified by using Matrix-Assisted Laser Desorption/Ionization-Time of Flight (MALDI-TOF) mass spectrometry (MS). The identified Acinetobacter isolates were tested to determine the antimicrobial resistance profiles towards 23 antibiotics using the disc diffusion method. A total of 13 Acinetobacter isolates were obtained from TMS (21.6%). Species distribution were as follow; Acinetobacter baumannii (n=9), Acinetobacter ursingii (n=3) and Acinetobacter iwoffii (n=1). Resistance to aztreonam, chloramphenicol, amoxicillin and clavulanate, cefoxitin, cefpodoxime gentamicin, cefuroxime, florphenicol was 53.8%,

Mustafa Kemal Üniversitesi Tıp Dergisi, Sep 26, 2022

Amaç: Demodex folliculorum (D. folliculorum) genellikle yüz ve kirpikler dahil olmak üzere insan ... more Amaç: Demodex folliculorum (D. folliculorum) genellikle yüz ve kirpikler dahil olmak üzere insan cildinde bulunan zorunlu bir ektoparazittir. Çalışmada Hatay huzurevi sakinlerinde D. folliculorum enfestasyonunun değerlendirilmesi amaçlandı. Gereç ve Yöntem: Çalışmaya huzurevinde kalan 81 yaşlı birey dahil edildi. Standart Yüzeyel Deri Biyopsi (SYDB) yöntemi ile yanak ve çene bölgelerinden örnekler alındı ve gliserin damlatılarak ışık mikroskobunda 10x-40x objektif büyütmesi ile D. folliculorum yönünden incelendi. Bulgular: Çalışmaya dahil edilen sakinlerin yaş ortalaması 74 olarak belirlendi. Huzurevi sakinlerinin %77,8’i D. folliculorum yönünden pozitif bulundu. Sakinlerin %84’ü hiperkolesterolemi, hipertansiyon, diyabet, KOAH-astım, kalp hastalıkları, böbrek hastalığı, romatizmal hastalıklar gibi kronik hastalıklara sahipti. Kronik rahatsızlığı bulunmayan %16’lık popülasyonun tamamı D. folliculorum açısından pozitifti. Sonuç: Kronik rahatsızlık ile D. folliculorum arasında anlamlı bir ilişki bulunamadı. Kronik hastalıkları olmasa dahi yaşlı sakinlerin immünsüpresif olduğu ve D. folliculorum enfestasyonunun immünsüpresyonla ilişkili olduğu göz ardı edilmemelidir.

Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi, Dec 20, 2022

Escherichia coli (E.coli) O157:H7 insan sağlığı açısından önemli morbidite ve mortalite nedenleri... more Escherichia coli (E.coli) O157:H7 insan sağlığı açısından önemli morbidite ve mortalite nedenlerinden biridir. E. coli O157:H7'ye bağlı enfeksiyonların çoğunluğu gıda kaynaklıdır. Bu çalışmada, Mersin ilinde tüketime sunulan çiğ sütlerde E. coli O157:H7 prevelansının tespiti amaçlanmıştır. Materyal ve Metot: Mersin ilinde çeşitli pazar ve bakkallarda satışa sunulan 60 adet çiğ süt örneği incelenmiştir. Her bir süt numunesi steril kaba alınmış olup, soğuk zincirde bakteriyoloji laboratuvarına getirilmiştir. Süt örneklerinden 25 ml olacak şekilde alınarak, zenginleştirme işlemi için 225 ml olarak hazırlanan Triptic Soy Broth'a inoküle edilmiştir. Zenginleştirme işlemi sonrasında, MacConkey veya Eozin Metilen Blue (EMB) agara kültüre edilmiştir. İnkübasyon sonunda üreyen şüpheli koloniler IMVIC testi ile E. coli olarak doğrulandıktan sonra E. coli O157:H7 araştırılması için Sorbitol-Mac-Conkey (SMAC) agar üzerine inoküle edilmiştir. Soyutlanan izolatlar antimikrobiyal direnç profilleri açısından araştırılmak üzere Clinical and Laboratory Standards Institute (CLSI) kılavuzlarına göre Kirby Bauer disk difüzyon yöntemi ile antibiyotik duyarlılık testi yapılmıştır. Bulgular: Analize alınan 60 adet çiğ süt örneğinin iki tanesinde (%3,3) E. coli O157:H7 soyutlanmıştır. E. coli O157:H7 izolatlarının ampisilin ve kloramfenikole karşı dirençli olduğu, gentamisin ve siprofloksasine ise yalnızca birinin dirençli iken diğer izolatın duyarlı olduğu bulundu. Ayrıca her iki izolatın da tetrasiklin, nalidiksik asit ve sefuroksime karşı duyarlı olduğu görüldü. Sonuç: Çiğ süt ve süt ürünlerinin E. coli O157:H7 için kaynak olabileceği ve halk sağlığı açısından risk teşkil edebileceği sonucuna varılmıştır.

Journal of Medical Entomology, Dec 21, 2018

Although demodicosis caused by Demodex folliculorum and Demodex brevis is widely seen throughout ... more Although demodicosis caused by Demodex folliculorum and Demodex brevis is widely seen throughout the world, the pathogenic mechanisms are not fully known. To the best of our knowledge, the effect of Demodex mites in patients with sickle cell anemia (SCA) is not known. SCA is a genetic disease characterized by abnormal hemoglobin production and suppression of the immune system. The aim of this study was to determine the prevalence and Demodex density in SCA patients and to compare with healthy subjects. The study included 70 patients diagnosed with SCA and control group of 50 healthy individuals. Samples were taken from cheeks, forehead, nose, and chin and were examined microscopically. Infestation of ≥5 mites/cm 2 was accepted as positive in the diagnosis. Demodex mite positivity was determined in 20 (28.6%) patients and none in subjects of the control group. In the SCA group, the mean number of mites was 26.10/cm 2. A statistically significant correlation was found between Demodex mite positivity and the number of SCA symptom attacks experienced by the patients within the last 1 yr (P ≤ 0.001). No significant relationship was determined between Demodex mite positivity and age or gender (P = 0.56 and P = 0.11, respectively). Demodex mites are seen more often in SCA patients who suffer from a compromised immune system, and the presence of Demodex mites could be a risk factor in the appearance of SCA symptom attacks.

Mustafa Kemal Üniversitesi Tıp Dergisi, Jul 26, 2022

The aim of this study was to determine the association between Clostridium difficile (C. difficil... more The aim of this study was to determine the association between Clostridium difficile (C. difficile) and vancomycin-resistant Enterococcus (VRE) and efficacy of screening stools submitted for C. difficile toxin assay for prevalence of VRE. Between April 2012 and February 2014, 158 stool samples submitted for C. difficile toxin to the Marmara University Microbiology Laboratory, were included in the study. Stool samples were analyzed by enzyme immuno assay test; VIDAS (bioMerieux, France) for Toxin A&B. Samples were inoculated on chromID VRE (bioMerieux, France) and incubated 24 h at 37 • C. Manuel tests and API20 STREP (bioMerieux, France) test were used to identify the Enterococci species. After the species identification, vancomycin and teicoplanin MIC's were performed by E test and molecular resistance genes for vanA vs vanB were detected by polymerase chain reaction (PCR). Of the 158 stool samples, 88 were toxin positive. The prevalence of VRE was 17%(n:19) in toxin positives however, 11.4% in toxin negatives(n:70). All VRE isolates were identified as Enterococcus faecium. These results were evaluated according to Fischer's exact chi-square test and p value between VRE colonization and C. difficile toxin positivity was detected 0.047 (p < 0.05). PPV and NPV were 79% and 47% respectively. In our study, the presence of VRE in C. difficile toxin positives is statistically significant compared with toxin negatives (p < 0.05). Screening for VRE is both additional cost and work load for the laboratories. Therefore VRE screening among C. difficile toxin positive samples, will be cost effective for determination of high risk patients in the hospitals especially for developing countries.

International Journal of Clinical Practice

Backgrounds. Diagnostic markers of extraintestinal infection in Escherichia coli (E. coli) remain... more Backgrounds. Diagnostic markers of extraintestinal infection in Escherichia coli (E. coli) remain unclear in the literature. Extraintestinal pathogenic E. coli (ExPEC) is differentiated from other E. coli isolates in terms of virulence factors, such as host cell adhesion, invasion, cytotoxic necrotizing factor (CNF (cnf1-cnf3)) and cytolethal distending toxin (CDT (cdt1-cdt4) that are responsible for cell death. We aimed to investigate the frequency of CNF-CDT and the relationship between the clinical diagnosis and genotypes in E. coli isolates with different clinical origins. Methods. A total of 646 E. coli isolates (obtained from 645 patients) isolated from different infection sites other than the intestine were evaluated in aspects of the CNF, CDT virulence genes, phylogenetic grouping, and phylogenetic relationship by using PCR and PFGE. Results. At least one virulence gene was present in 156 (24%) of the 646 ExPEC isolates. We detected cnf1, cnf2, and cnf3, in 78, 12, and 20 Ex...

Background: Determinants of extraintestinal infection in Escherichia coli (E. coli) remain unclea... more Background: Determinants of extraintestinal infection in Escherichia coli (E. coli) remain unclear. Virulence factors making Extraintestinal pathogenic E. coli (ExPEC) different from other E. coli strains are the host cell adhesion, invasion, and two important factors/toxins, Cytotoxic Necrotizing Factor (CNF) and Cytolethal Distending Toxin (CDT) that are responsible for cell death. In the present study, prevalence of CNF-CDT genotypes was investigated in 645 E. coli strains isolated from patients. This prevalence was analyzed regarding clinical origins, phylogroups and putative phylogenetic relationships.Results: At least one virulence gene identified for ExPEC was found in 156 (24%) of 645 E. coli strains. 78, 12, 20 of ExPEC strains contained cnf1, cnf2, cnf3, respectively. Genes of cdt1, cdt2, cdt3 and cdt4 were detected as 20, 4, 4, 4. Finally, two factors were detected cnf1-cnf3 (n=6), cnf1- cdt1 (n=4), cdt1-cdt4 (n=4). These 156 strains were found to be distributed in 106 la...

Journal of Clinical Medicine of Kazakhstan

Aim: This study aimed to determine the susceptibility of carbapenem-resistant Gr (-) bacilli isol... more Aim: This study aimed to determine the susceptibility of carbapenem-resistant Gr (-) bacilli isolated from various clinical infections to various antibiotics and identify genes causing carbapenem resistance and their clonal relationships to elucidate the distribution of resistance in community and/or hospital-acquired strains. Material and methods: In this study, antibiotic susceptibilities of 450 carbapenem-resistant Gr (-) bacilli isolated from clinical specimens at Cukurova University, Faculty of Medicine, Balcali Hospital, were investigated using phenotypic methods. The presence of carbapenems and β-lactamase genes were searched using polymerase chain reaction (PCR) and sequence analysis methods. Pulsed-field gel electrophoresis (PFGE) method was used to evaluate the phylogenetic relationship of the isolates. Results: Based on the results, it was determined that 99.23% of the strains had gained resistance to meropenem, whereas 5.38% had developed resistance to colistin. The most...

Mediterranean Journal of Infection Microbes and Antimicrobials

Staphylococci are microorganisms that are resistant to many antibiotics. Staphylococcus epidermid... more Staphylococci are microorganisms that are resistant to many antibiotics. Staphylococcus epidermidis is a normal flora bacterium that has recently emerged as a nosocomial pathogen and has become a worldwide issue. The aim of the study was to determine the prevalence of coagulase negative staphylococci, Staphylococcus aureus nasal carriage, and antibiotic resistance of these bacteria in nursing home residents. Materials and Methods: This study was conducted in July 2017. Forty-six samples obtained from the nasal mucosa of the participants were cultured. The species identification was done by MALDI-TOF-MS. Disk diffusion was applied to determine the antibiotic susceptibility of isolates, and polymerase chain reaction assay was employed to identify the known antimicrobial resistance genes. Results: Staphylococci were isolated from 28 (61%) of the 46 residents. The isolates (n=29) were S. epidermidis (n=22), Staphylococcus succinus (n=4), and S. aureus (n=3). Two different strains of S. epidermidis were isolated from one participant. While methicillin-resistant S. aureus was not identified in isolates; Methicillin-resistant S. epidermidis (MRSE) was found in 43% (n=12). In addition, 68% of S. epidermidis strains were multidrug resistant (MDR) (to at least one agent in three or more antimicrobial groups other than β-lactams). All methicillin-resistant S. epidermidis (MRSE) isolates were mecA positive. Among the MRSE isolates (n=12), the following resistance genes were found: blaZ (n=5), lnuA (n=1),tetK (n=1), ermA (n=1), aac(6')/aph(2'') (n=1), and ant(4')-Ia (n=1). Conclusion: To our knowledge, multi-drug resistant MRSE was detected in nursing home residents for the first time in Turkey with this study. This result suggests that S. epidermidis could serve as a reservoir of drug resistance by persistent colonization in the nasal mucosa. Observation and molecular surveillance could be applied to limit the spread of such resistant nosocomial pathogens.

Journal of Clinical Medicine of Kazakhstan

Aim: Patients infected with the hepatitis B virus (HBV) are at a higher risk of cirrhosis and hep... more Aim: Patients infected with the hepatitis B virus (HBV) are at a higher risk of cirrhosis and hepatocellular carcinoma. Despite the recent advancement of antiviral therapy, many patients still cannot respond to existing therapies. Hence, to detect the changes in liver function earlier, non-invasive methods are needed. Long non-coding RNAs (lncRNAs) play important roles in essential biological process as well as human cancer. LncRNAs may be used as biomarkers in human diseases. Thus, in this study, we purposed to analyze the expression levels of lncRNAs (HOX transcript antisense RNA (HOTAIR), maternally expressed 3 (MEG-3), highly upregulated in liver cancer (HULC)) in patients with hepatitis B virus and healthy volunteers. Methods: We selected three lncRNAs as candidate lncRNAs based on their association with liver disease. Whole blood samples were collected from 40 patients with HBV and 48 healthy volunteers. The expression levels of all the samples were evaluated by quantitative r...

CERN European Organization for Nuclear Research - Zenodo, Oct 1, 2022

Aim: Our research was carried out to determine the knowledge and attitudes of university students... more Aim: Our research was carried out to determine the knowledge and attitudes of university students about cervical cancer, human papilloma virus (HPV) infection and vaccine. Materials and Methods: In the 2018-2019 academic year, the research population consisted of 178 female students who studied at Hatay Mustafa Kemal University, Vocational School of Health Services. Students completed a form that included socio-demographic information as well as questions about HPV infection, vaccination, and Attitude Scale Towards Preventing Cervical Cancer (ASTPCC). For the analysis of the data, percentage, mean, T-test, One-Way ANOVA test and Tukey test were used. Significance level was taken as 0.05. Results: Of the female students included in the study, 171 (96%) people between the ages of 18-25 and 7 (4%) people between the ages of 26-32 were recorded. 94% of participants said that HPV is a risk factor for cervical cancer, 92% said that HPV is transmitted through sexual contact, 87% said that the HPV vaccine protects against cervical cancer, 19% said that the HPV vaccine should be applied before the first sexual activity, and 9% said that regular pap smear tests should be performed even if they have received the HPV vaccine. While of the students, 36% had no knowledge about HPV-associated cervical cancer, 30% had no knowledge pap-smear test. Conclusion: Female students at university should be aware of HPV infection, HPV-associated cervical cancer, pap-smear test, and vaccination. Developing healthy lifestyle habits during university education is essential. ARTICLE INFO/MAKALE BİLGİSİ

TEZ11644Tez (Doktora) -- Çukurova Üniversitesi, Adana, 2017.Kaynakça (s. 68-84) var.x, 85 s. : re... more TEZ11644Tez (Doktora) -- Çukurova Üniversitesi, Adana, 2017.Kaynakça (s. 68-84) var.x, 85 s. : res. (bzs. rnk.), tablo ; 29 cm.Escherichia coli‘de (E.coli) ekstraintestinal enfeksiyon tanı markırları hala belirsizliği korumaktadır. Ekstraintestinal patojenik E.coli'yi (ExPEC) diğer E.coli suşlarından farklı kılan virülans faktörleri konak hücreye adezyon, invazyon ve hücre ölümünden sorumlu olan iki önemli faktör/toksin: Cytotoxic Necrotizing Factor (CNF) ve Cytolethal Distending Toxin’dir. Bu çalışmada, intestinal sistem dışındaki enfeksiyon odaklarından izole edilen 645 E. coli suşunda CNF-CDT genotip prevelansları araştırıldı. Bu prevalans, örneklerin klinik kökenleri, filogrupları ve filogenetik ilişkileri ile analiz edildi. Analiz edilen 645 E. Coli suşunda ExPEC için tanımlanan virülans genlerinden (CNF1-3/CDT1-4) en az birini taşıyan 156 suş bulundu. Sırasıyla; ExPEC suşlarının 81, 20 ve 18'i cnf1, cnf2, cnf3; 20, 4, 4 ve 4’ü cdt1, cdt2, cdt3, cdt4 içermekteydi. İkili...

Acta Microbiologica et Immunologica Hungarica

Group A streptococci are important pathogens with various virulence factors, such as M protein, s... more Group A streptococci are important pathogens with various virulence factors, such as M protein, superantigens, hemolysins, deoxyribonuclease, and proteases. The aims of this study are to investigate the detection of emm genotypes and other virulence genes, such as SAgs, DNase, protease, antibiotic resistance, and phylogenetic relationships in GAS strains isolated from clinical samples.Test strains were obtained from Çukurova University Balcalı Hospital and regional hospitals in Adana province. The M proteins were detected by sequence analysis of emm genes. SAgs and other virulence gene profiles were determined using the Multiplex-PCR method. The antibiotic susceptibility of the isolates was performed by the disc diffusion method and evaluated according to CLSI criteria. The PFGE method was used to determine the clonal relationship between the strains.The emm gene was positive in 86 isolates. The most common emm genotypes were emm28 (22%), emm1 (18.6%), emm12 (13.9%), and emm3 (11.6%...

Aim: Although the antimicrobial properties of essential oils have been known for a long time they... more Aim: Although the antimicrobial properties of essential oils have been known for a long time they have not been used for surgical hand antisepsis so far. The aim of this study was to test the surgical hand antiseptic properties of Origanum minutiflorum essential oil. Methods: Surgical hand antiseptics containing 0, 4, 8, 16, and 32% essential oil were compared with povidone-iodine. Fifteen volunteers were assigned for each antiseptic solution. Fingertip imprints were taken on agar plates before treatment (t0), just after hand treatment (t1) and three hours after gloving with sterile powder-free surgical gloves (t2). The bacterial colony counts and bacterial genus number were evaluated. Results: No antibacterial effect was seen in 0% oregano essential oil group. The results in 32% essential oil group and povidone-iodine group were similar. Bacterial colony counts reduction in povidone-iodine scrub group was greater than the 16% essential oil group at t1, but the difference between both groups disappeared at t2. The reductions of bacterial genus number at t1 in povidone-iodine scrub group was higher than the 4% essential oil group, but it was similar at t2. Conclusion: Oregano essential oil has strong and long-acting antiseptic properties on hand pathogens. Hand treatment with 32% oregano essential oil containing hand antisepsis is equivalent to the hand antisepsis with conventional povidone-iodine scrub. Oregano essential oil containing antiseptic solutions have very strong effects in reducing the bacterial genus number even in low concentrations. Oregano essential oil containing hand antisepsis could be suggested for biodegradable and eco-friendly natural microbicidal activities

Van Medical Journal, 2019

Konuralp Tıp Dergisi, 2018

Objective: Lung cancer is the deadliest cancer type worldwide. Poor prognosis of lung cancer pati... more Objective: Lung cancer is the deadliest cancer type worldwide. Poor prognosis of lung cancer patients and lack of an effective treatment require detailed understanding of lung cancer pathogenesis. It was highlighted in some studies that U-II is likely to be a biomarker or molecular target for the prevention and treatment of some diseases such as lung cancer. But its molecular action mechanism has not been elucidated yet. In the present study, we aimed to investigate the role of U-II in lung cancer. Methods: In our study, A549 cells were induced with different doses of IL-1β at different durations (1, 3 ng/ml; 6, 24 hours). mRNA levels of GAPDH, NF-κB1, MMP-1, and U-II were analyzed with RT-qPCR. The Delta Delta Ct (∆∆Ct) method was used for data analysis. The analyzed data were expressed as the "foldchange". Results: Our results indicate that U-II gene is expressed in A549 cells and IL-1β can induce gene expressions of U-II, MMP-1 and NF-κB1 in A549 cells. Conclusions: U-II is a promising molecular target in treatment and prevention of lung cancer. Therefore, further studies are needed to enlighten molecular mechanism of U-II in lung adenocarcinoma.

Science of The Total Environment, 2022

AIMS The increasing number of globally established fosfomycin-resistant (FosR) Gram-negative bact... more AIMS The increasing number of globally established fosfomycin-resistant (FosR) Gram-negative bacteria inspired us to investigate the occurrence of FosREnterobacterales populations (esp. E. coli) in samples of city wastewater treatment plants (WWTPs) and hospital sewage in Hatay, Turkey. FosR target bacteria were further characterized for their clonal relatedness, resistomes and mobile genetic elements (MGEs) to evaluate their impact on fosfomycin resistance dissemination. METHODS A total of 44 samples from raw and treated waters of WWTPs as well as of two hospitals in the Hatay province were subjected to selective cultivation for recovering FosREnterobacterales. The presence of fosA was verified by PCR and Sanger amplicon sequencing. Detected E. coli were further evaluated against antimicrobial susceptibility-testing, macrorestriction profiling (PFGE) and whole-genome sequencing (WGS). Bioinformatics analysis was performed for genome subtyping (i.e., MLST, serotype), resistome/virulome determination and dissection of the genetic determinants of plasmidic fosA3/4 resistances. RESULTS Besides ten non-E. coli Enterobacterales, 29 E. coli were collected within this study. In silico-based subtyping revealed that E. coli isolates were assigned to six different serovars and 14 sequence types (ST), while O8:H21 and ST410 represented the major prevalent types, respectively. Fosfomycin resistance in the isolates was found to be mediated by the fosA4 (n = 18), fosA3 (n = 10) and fosA (n = 1), which are frequently associated with transmissible MGEs. Reconstruction of plasmid-associated fosA gene context revealed a linkage between the resistance cassette and IS6 (IS26 family) transposases, which might represent a major driver for the distribution of the genes and the generation of novel fosA-carrying plasmids. CONCLUSIONS The occurrence of plasmid-mediated, transmissible FosR in E. coli from wastewater pose a foreseeable threat to "One-Health". To minimize further spread of the resistances in bacterial populations associated with environmental, animal and human health further resistance monitoring and management strategies must be developed.

Journal of Clinical Medicine of Kazakhstan

Objective: M protein is an important marker in epidemiological and phylogenetic surveillance of B... more Objective: M protein is an important marker in epidemiological and phylogenetic surveillance of Beta-Hemolytic Streptococci. The aim of this study is to determine emm types and their distribution among the Group C/G streptococci (GCG/GGS) strains isolated from clinical samples. Material and methods: The study includes 98 β-hemolytic streptococcus strains isolated from clinical samples in Çukurova University/Balcalı Hospital. 67 of these isolates, defined as serologic, of which 56 were identified as Streptococcus dysgalactiae subsp. equisimilis, 8 as S. anginosus and 3 as S.equi subsp.zooepedimicus, have been included in the study. These strains were also confirmed by the Vitek 2GP-ID system. These isolates were confirmed by the polymerase chain reaction (PCR) method with primers based on groESL sequences. PCR-sequence analysis method developed by the Centers for Disease Control (CDC) was used for typing according to emm polymorphism. Results: 56/67 (83.6%) isolates in which emm-PCR was determined as positive were typed by sequence analysis. The findings were identified using the CDC database, and the most common type was determined to be stG485 (51.85%), stG643 (12.96%) and stG6 (9.25%). Conclusion: Consequently, GCS/GGS should be treated more seriously in our country as in the whole world.

Kafkas üniversitesi veteriner fakültesi dergisi/Kafkas üniversitesi veteriner fakültesi dergisi, 2024

DergiPark (Istanbul University), May 15, 2023

Zenodo (CERN European Organization for Nuclear Research), Jan 29, 2023

Mustafa Kemal Üniversitesi Tıp Dergisi, Sep 26, 2022

Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi, Dec 20, 2022

Journal of Medical Entomology, Dec 21, 2018

Mustafa Kemal Üniversitesi Tıp Dergisi, Jul 26, 2022

International Journal of Clinical Practice

Journal of Clinical Medicine of Kazakhstan

Mediterranean Journal of Infection Microbes and Antimicrobials

Journal of Clinical Medicine of Kazakhstan

CERN European Organization for Nuclear Research - Zenodo, Oct 1, 2022

Acta Microbiologica et Immunologica Hungarica

Van Medical Journal, 2019

Konuralp Tıp Dergisi, 2018

Science of The Total Environment, 2022

Journal of Clinical Medicine of Kazakhstan