Ashwini Appaji | Dayanand Sagar Institutions (original) (raw)
Papers by Ashwini Appaji
Exacum bicolor, an endemic and endangered medicinal plant, belongs to the family Gentianaceae. A ... more Exacum bicolor, an endemic and endangered medicinal plant, belongs to the family Gentianaceae. A rapid protocol has been developed for efficient multiple shoot induction by testing nodal explants on Murashige and Skoog (MS) medium supplemented with various cytokinins. The cy-tokinins 6-benzyladenine (BA), 6-furfurylaminopurine (Kn), 2-isopentenyladenine (2-iP) and zeatin (Zn) were used individually and in combination at different concentrations (0.5, 1.0, 2.0, 5.0, and 10.0 μM). The maximum number of shoots (19.33±1.09 per explant) as well as their fresh weight (5.1± 0.68 g) and dry weight (216.83±2.84 mg) were obtained with 10.0 μM BA+2.0 μM Kn. After 4 wk, the multiple shoots from agar culture were subcultured into liquid medium containing the same growth regulator combinations. After 8 wk of liquid culture, the best treatment had about tenfold increase in shoot number (199.5±1.14 per explant). The fresh weight (13.76 ± 0.14 g) and dry weight (909.33± 1.92 mg) were highest with full strength MS medium supplemented with 3% sucrose and containing 10.0 μM BA+2.0 μM Kn. Maximum root development was observed after 30 d with 0.5 μM indole-3-butyric acid (IBA) supplementation. Regenerated plants were successfully transferred to pots containing coco peat:perlite mixture and showed a 75% survival rate. Genetic fidelity of in vitro plantlets compared to mother plant were assessed using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers. All the regenerated plants were genetically identical to their mother plant, showing no detectable genetic variation in the regenerated plantlets. Thus, this protocol could be successfully used for mass multiplication and germplasm conservation of E. bicolor.
Objective: To investigate different phytochemical contents and screening of antioxidant activity ... more Objective: To investigate different phytochemical contents and screening of antioxidant activity of sequentially extracted Exacum bicolor leaf with different solvents. Methods: Leaf samples of E. bicolor were dried and subjected to sequential (hexane>chloroform>ethyl acetate>methanol>water) extraction using soxhlet apparatus. Total phenol contents (TPC) were estimated using Folin-ciocalteau method, flavonoids by aluminium chloride method, tannins by Folin-Denis reagent and alkaloids using bromocresol blue. In vitro antioxidant activities were estimated by using 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid ABTS ·+ and Nitric oxide (NO) scavenging assay. Results: The total phenol, tannin and alkaloid contents in methanol extract were found to be 23.4±0.09 mg GAE/g, 23±0.57 mg TAE/g and 47.33±1.20 mg AE/g respectively. Hexane extract exhibited high flavonoid content (27.6±0.88 mg QE/g). Methanolic extracts exhibited dose dependent antioxidant activity against DPPH, ABTS ·+ and NO Scavenging methods; low IC50 value (14.84 µg/ml) was observed in DPPH scavenging activity. Findings showed positive and significant (P ≤ 0.05) correlation between TPC/DPPH (R 2 Keywords: Exacum bicolor, DPPH, ABTS = 0.98). Conclusions: The methanol extract showed higher antioxidative activity compared to other extracts. Positive correlation was obtained between TPC and DPPH activity signifying that the phenolic content may be responsible for the antioxidant activity of the plant. Further bioassay-guided purification of the bioactive compound is necessary. To the best of our knowledge, antioxidant activity and its correlation with TPC, is reported for the first time in E. bicolor.
Exacum bicolor Roxb. is a deciduous herbaceous plant which is endemic to peninsular India. Due to... more Exacum bicolor Roxb. is a deciduous herbaceous plant which is endemic to peninsular India. Due to its bitterness in taste local people use as herbal remedy for diabetes and skin disorder and also used for stomachic, febrifuge and antifungal diseases. The present study is designed to evaluate the qualitative phytochemical analysis and anthelmintic activity of extracts of leaves of Exacum bicolor. Extraction of the plant materials were carried out sequentially using different solvents (hexane>chloroform>ethyl acetate>methanol>water). Pheretima postuma was used as test organism for anthelmintic activity. Different concentrations (5, 10, 15 mg/ml) of all the extracts were tested for activity. The time of paralysis and time of death were studied and the activity was compared with albendazole (10mg/ml) as reference standard. Qualitative analysis confirmed the presence of phenols, flavonoids, alkaloids, cardiac glycosides, saponin, steroids and terpenoids in detectable amounts. It was found that all the extracts showed dose-dependent anthelmintic activity but the hexane extract exhibited better inhibition of spontaneous motility (paralysis) and death of earthworms when compared to reference standard (P < 0.0001). The potent extract was partially purified by TLC and 6 bands were visualized where band no. 4 showed lesser time of paralysis (3.186 ±0.14 min) and time of death (5.324±0.11 min) at 10mg/ml when tested for anthelmintic activity. From the present study, it can be concluded that E. bicolor possesses potent anthelmintic activity. As per literature, this is the first scientific report on the anthelmintic properties of leaf extracts of Exacum bicolor.
![Research paper thumbnail of Synthesis and Molecular Docking Study of Novel Pyrazolo[3,4-b]quinoline Derivatives 1a-d 4a-e, 9](https://attachments.academia-assets.com/54646520/thumbnails/1.jpg)
](Phenylpyrazolo[3,4-b]quinolin-3-ols were prepared by using 2-chloroquinoline-3-carboxylic acids a... more Phenylpyrazolo[3,4-b]quinolin-3-ols were prepared by using 2-chloroquinoline-3-carboxylic acids and phenyl hydrazine hydrochlorides in the presence of POCl 3. One of the phenylpyrazolo[3,4-b]quinolin-3-ols underwent chlorination (9). To check binding modes and binding affinity of synthesized compounds were docked with the active sites of human telomerase (hTERT). The results indicated that compound 4b has good affinity to the active site residue of human telomerase, least energy (-23.012 score).
Exacum bicolor, an endemic and endangered medicinal plant, belongs to the family Gentianaceae. A ... more Exacum bicolor, an endemic and endangered medicinal plant, belongs to the family Gentianaceae. A rapid protocol has been developed for efficient multiple shoot induction by testing nodal explants on Murashige and Skoog (MS) medium supplemented with various cytokinins. The cy-tokinins 6-benzyladenine (BA), 6-furfurylaminopurine (Kn), 2-isopentenyladenine (2-iP) and zeatin (Zn) were used individually and in combination at different concentrations (0.5, 1.0, 2.0, 5.0, and 10.0 μM). The maximum number of shoots (19.33±1.09 per explant) as well as their fresh weight (5.1± 0.68 g) and dry weight (216.83±2.84 mg) were obtained with 10.0 μM BA+2.0 μM Kn. After 4 wk, the multiple shoots from agar culture were subcultured into liquid medium containing the same growth regulator combinations. After 8 wk of liquid culture, the best treatment had about tenfold increase in shoot number (199.5±1.14 per explant). The fresh weight (13.76 ± 0.14 g) and dry weight (909.33± 1.92 mg) were highest with full strength MS medium supplemented with 3% sucrose and containing 10.0 μM BA+2.0 μM Kn. Maximum root development was observed after 30 d with 0.5 μM indole-3-butyric acid (IBA) supplementation. Regenerated plants were successfully transferred to pots containing coco peat:perlite mixture and showed a 75% survival rate. Genetic fidelity of in vitro plantlets compared to mother plant were assessed using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) markers. All the regenerated plants were genetically identical to their mother plant, showing no detectable genetic variation in the regenerated plantlets. Thus, this protocol could be successfully used for mass multiplication and germplasm conservation of E. bicolor.
Objective: To investigate different phytochemical contents and screening of antioxidant activity ... more Objective: To investigate different phytochemical contents and screening of antioxidant activity of sequentially extracted Exacum bicolor leaf with different solvents. Methods: Leaf samples of E. bicolor were dried and subjected to sequential (hexane>chloroform>ethyl acetate>methanol>water) extraction using soxhlet apparatus. Total phenol contents (TPC) were estimated using Folin-ciocalteau method, flavonoids by aluminium chloride method, tannins by Folin-Denis reagent and alkaloids using bromocresol blue. In vitro antioxidant activities were estimated by using 1,1-Diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid ABTS ·+ and Nitric oxide (NO) scavenging assay. Results: The total phenol, tannin and alkaloid contents in methanol extract were found to be 23.4±0.09 mg GAE/g, 23±0.57 mg TAE/g and 47.33±1.20 mg AE/g respectively. Hexane extract exhibited high flavonoid content (27.6±0.88 mg QE/g). Methanolic extracts exhibited dose dependent antioxidant activity against DPPH, ABTS ·+ and NO Scavenging methods; low IC50 value (14.84 µg/ml) was observed in DPPH scavenging activity. Findings showed positive and significant (P ≤ 0.05) correlation between TPC/DPPH (R 2 Keywords: Exacum bicolor, DPPH, ABTS = 0.98). Conclusions: The methanol extract showed higher antioxidative activity compared to other extracts. Positive correlation was obtained between TPC and DPPH activity signifying that the phenolic content may be responsible for the antioxidant activity of the plant. Further bioassay-guided purification of the bioactive compound is necessary. To the best of our knowledge, antioxidant activity and its correlation with TPC, is reported for the first time in E. bicolor.
Exacum bicolor Roxb. is a deciduous herbaceous plant which is endemic to peninsular India. Due to... more Exacum bicolor Roxb. is a deciduous herbaceous plant which is endemic to peninsular India. Due to its bitterness in taste local people use as herbal remedy for diabetes and skin disorder and also used for stomachic, febrifuge and antifungal diseases. The present study is designed to evaluate the qualitative phytochemical analysis and anthelmintic activity of extracts of leaves of Exacum bicolor. Extraction of the plant materials were carried out sequentially using different solvents (hexane>chloroform>ethyl acetate>methanol>water). Pheretima postuma was used as test organism for anthelmintic activity. Different concentrations (5, 10, 15 mg/ml) of all the extracts were tested for activity. The time of paralysis and time of death were studied and the activity was compared with albendazole (10mg/ml) as reference standard. Qualitative analysis confirmed the presence of phenols, flavonoids, alkaloids, cardiac glycosides, saponin, steroids and terpenoids in detectable amounts. It was found that all the extracts showed dose-dependent anthelmintic activity but the hexane extract exhibited better inhibition of spontaneous motility (paralysis) and death of earthworms when compared to reference standard (P < 0.0001). The potent extract was partially purified by TLC and 6 bands were visualized where band no. 4 showed lesser time of paralysis (3.186 ±0.14 min) and time of death (5.324±0.11 min) at 10mg/ml when tested for anthelmintic activity. From the present study, it can be concluded that E. bicolor possesses potent anthelmintic activity. As per literature, this is the first scientific report on the anthelmintic properties of leaf extracts of Exacum bicolor.
Phenylpyrazolo[3,4-b]quinolin-3-ols were prepared by using 2-chloroquinoline-3-carboxylic acids a... more Phenylpyrazolo[3,4-b]quinolin-3-ols were prepared by using 2-chloroquinoline-3-carboxylic acids and phenyl hydrazine hydrochlorides in the presence of POCl 3. One of the phenylpyrazolo[3,4-b]quinolin-3-ols underwent chlorination (9). To check binding modes and binding affinity of synthesized compounds were docked with the active sites of human telomerase (hTERT). The results indicated that compound 4b has good affinity to the active site residue of human telomerase, least energy (-23.012 score).