hadi pourtaghi | Islamic Azad University, Karaj Branch (original) (raw)

Papers by hadi pourtaghi

Virology Journal

Canine parvovirus-2 (CPV-2) is a virus with worldwide spread causing canine gastroenteritis. New ... more Canine parvovirus-2 (CPV-2) is a virus with worldwide spread causing canine gastroenteritis. New strains of this virus have unique characteristics and are resistant to some vaccine strains. Therefore, understanding the root causes of resistance has proven to be of increasing concern to many scientists. This study collected 126 whole genome sequences of CPV-2 subtypes with specific collection dates from the NCBI data bank. The whole genome sequences of CPV-2 collected from different countries were analyzed to detect the new substitutions and update these mutations. The result indicated 12, 7, and 10 mutations in NS1, VP1, and VP2, in that respective order. Moreover, the A5G and Q370R mutations of VP2 are the most common changes in the recent isolates of the CPV-2C subtype, and the new N93K residue of VP2 is speculated to be the cause of vaccine failure. To summarize, the observed mutations, which are increasing over time, causes several changes in viral characteristic. A comprehensiv...

archives of razi institute, Apr 1, 2022

BMC Veterinary Research

Background Canine Parvovirus type 2 (CPV-2) is a member of the Parvoviridae family with a global ... more Background Canine Parvovirus type 2 (CPV-2) is a member of the Parvoviridae family with a global distribution and causes pathogenicity in puppies aged from 6 weeks to 6 months. It should be noted that Maternally Derived Antibodies (MDA) have protection against CPV-2 in the first weeks of puppies’ life. However, MDA declines with age. The most important influential factor is timely vaccination against CPV-2. Methods In this study, 24 healthy 8-week-old terrier puppies were selected and divided into three identical groups based on a randomized, double-blind comparative trial. One of which was called the control group that was injected with the physiological serum. The second group was the group A that was vaccinated by the vaccine provided by Biocan DHPPi+L (Bioveta, Czech). The third group was group B that was vaccinated by the vaccine of Duramune Max 5 + LCI / GP (Fort Dodge Animal Health, USA) from 8 to 16 weeks of their life at every 4 weeks. Then serum samples were analyzed with ...

Iranian journal of veterinary research, 2021

Background Canine parvovirus type 2 (CPV-2) causes gastroenteritis and leukopenia in dogs worldwi... more Background Canine parvovirus type 2 (CPV-2) causes gastroenteritis and leukopenia in dogs worldwide. They are three subtypes of CPV-2 including CPV-2a, CPV-2b, and CPV-2c. The distribution status of CPV-2 subtypes has been shown differences in many countries. Aims The aim of the present study was detection and phylogenetic analysis of different subtypes of CPV-2 circulating in two provinces of Iran, Tehran and Alborz. Methods CPV-2 was detected using 555 primer pairs in collected samples. Phylogenetic analysis of CPV-2 subtypes was done using sequencing of the partial length of VP2 gene. Results Twenty-eight CPV-2 were detected using 555 primer pair. The sequences of isolates were deposited in the GenBank database. Phylogenetic analysis revealed that all CPV-2c subtype isolates had very high sequence identity to China and Zambia that form a distinct cluster. Conclusion In conclusion, this study revealed the emergence of all CPV-2 variants in dogs in Iran. Thus, the continual monitor...

Veterinary medicine and science, Jan 9, 2023

Veterinary Medicine and Science

Background: Avian influenza type A viruses (AIV) can infect a broad range of hosts including huma... more Background: Avian influenza type A viruses (AIV) can infect a broad range of hosts including human and birds, making them an important viral pathogen with zoonotic potential. Ducks are a known reservoir for many avian viruses including the AIV. Objectives: To sequence the entire genome of duck-derived H3N2 and ran comprehensive phylogenetic analysis on them to study their origin. Methods: In this study, 962 cloacal swabs were collected from domestic ducks at several live poultry markets (LPMs) of Gilan, Mazandaran and Golestan provinces of Iran in the year 2017. Results: Preliminary assays such as haemagglutination inhibition assay (HI), Neuraminidase Inhibition assay(NI) and RT-qPCR suggested that 0.5% of the birds were infected by H3 low pathogenic influenza viruses (LPAI). Three isolates were selected for whole genome sequencing. The cleavage site of the HA genes showed a PEKQTR/GLF motif, an indicator of LPAI. Furthermore, BLAST and phylogenetic analyses of the HA gene showed high homology to the Eurasian lineage of H3N8 AIV (95.5%-97.1% to several European and East Asian isolates). However, the NA genes showed high homology (at most 96.5-96.9%) to those belonging to AIV N2 subtype. Furthermore, internal genes showed high homology (96%-98%) to a variety of duckorigin subtypes and glycoprotein combinations, which were different for each segment. This showed a complex reassortment between different subtypes. Discussion: This report is the first whole genome sequencing and complete characterisation of H3N2 AIV from Iran. Conclusion: Such surveillance should continue to study the evolution and possible emergence of viruses with pandemic potential. K E Y W O R D S avian influenza virus, domestic ducks, H3 subtype, Iran, live poultry market This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

شمارش سلولهای سوماتیک (somatic cell count; SCC) شیر یک روش معمول در ارزیابی کیفیت شیر تولیدی و ب... more شمارش سلولهای سوماتیک (somatic cell count; SCC) شیر یک روش معمول در ارزیابی کیفیت شیر تولیدی و بررسی وضعیت ورم پستان میباشد. ماساژ سبب افزایش سیستم ایمنی سلولی می‌شود.هدف از انجام مطالعه حاضر، بررسی تاثیر ماساژ پستان بر سیستم ایمنی پستان، با توجه به تعداد سلولهای سوماتیک و میزان تولید شیر بود.برای این منظور تعداد 14 رأس گاو پرتولید با سن حدود 3 سال که مدت 3 ماه از زایمانشان گذشته بود، انتخاب شدند. سپس در 2 گروه شاهد و تیمار تقسیمبندی شدند. گروه تیمار، روزانه دو بار و به مدت 4 هفته، ماساژ را در ناحیه پستان دریافت کردند. در طی دوره آزمایش نیز هر هفته شمارش سلولهای سوماتیک انجام میشد. مقدار تولید شیر نیز یکبار در روز صفر و بار دیگر در هفته ششم که هفته آخر ماساژدهی در گروه تیمار بود، اندازهگیری شد. یافتهها حاکی از افزایش چشمگیر ولی غیرمعنیدار تعداد سلولهای سوماتیک (05/0p >) و کاهش معنیدار میزان تولید شیر در گاوهای گروه تیمار بود (05/0p >). اما میانگین تفاوت تولید شیر در بین گروههای تیمار و کنترل در پایان هفته ششم نسبت به هفته اول، اختلاف آماری معنیداری را نشان د...

Advanced Studies in Biology, 2015

This study was performed to estimate the prevalence of Enterotoxigenic Escherichia coli (ETEC) in... more This study was performed to estimate the prevalence of Enterotoxigenic Escherichia coli (ETEC) in calves’ diarrhoea samples by molecular and serological methods. Rectal swabs from 127 diarrhoeaic calves were collected from 10 different farms of Tehran province during March to September 2014. ETEC was detected by both mPCR and ELISA. According to the results, ETEC was detected in 14 (11%) and 5 (3.9%) diarrhoea samples by mPCR and ELISA methods respectively. All 14 positive samples were detected by mPCR method had three virulence genes including K99 (F5), F41 and STa. Although 11 (78.5%) and 3 (21.4%) positive cases were identified from traditional and semi-industrial farms respectively, no isolates were identified related to the industrial farms. The results represented that detection assays such as ELISA which is only able to detect fimbriae (F5), will follow false-positive cases. For this reason mPCR assay has been developed and recognized as effective, rapid and reliable tools fo...

Norwalk virus is the major cause of food borne gastroenteritis worldwide with the highest infecti... more Norwalk virus is the major cause of food borne gastroenteritis worldwide with the highest infections by GII genotype. The burden of Norwalk virus related disease is considerable, affecting all age groups around the world. Norwalk virus is extremely contagious pathogen which can simply transmitted by consuming contaminated food and lead to serious outbreak gastroenteritis disease. The aim of this study was to investigate the incidence of Norwalk virus contamination in Bivalvia in the Northern Persian Gulf (southwest of Iran). All samples which examined for Norwalk virus (norovirus) contaminations were negative as investigated and verified by reverse transcriptasepolymerase chain reaction (RT-PCR) tests. The results of PCR products on gel electrophoresis indicated that there were no norovirus contaminations in tested samples. Our investigation indicated that molluscs from southwest of Iran are not contaminated with norovirus, however, it would be wisely to eat molluscs with adequate c...

Iranian journal of veterinary research, 2017

The aim of this study was to identify virulence genes and antimicrobial resistance of Escherichia... more The aim of this study was to identify virulence genes and antimicrobial resistance of Escherichia coli isolated from bovine clinical mastitis in dairy herds in Iran. Sampling was done from 86 inflamed quarters of dairy cows in 8 commercial farms of Alborz province, Iran in summer 2015. Shiga toxin-producing E. coli (STEC) virulence genes were detected by multiplex PCR and multi-drug resistance profiles were confirmed using disk diffusion method. Among 60 E. coli isolated from examined samples, 13 (21.6%) of them were STEC. The results of PCR assay showed that eaeA gene was carried by 4 (30.8%) of STEC isolates. Although stx1 in combination with eaeA gene was detected from 7 (53.8%) of STEC isolates, stx1 and stx2 genes were detected from only 1 (7.7%) of the examined samples. The result of the disk diffusion method showed that all E. coli isolates were resistant to penicillin, tylosin, oxytetracycline, erythromycin, ampicillin, streptomycin and neomycin. However all isolates were su...

Avian influenza type A viruses (AIV) can infect a broad range of hosts including human and birds,... more Avian influenza type A viruses (AIV) can infect a broad range of hosts including human and birds, making them an important viral pathogen with zoonotic potential. Among birds, ducks are a known reservoir for many avian viruses including the AIV. During migration seasons in Iran, this bird species is generally at a high risk of being infected by free-living aquatic birds. In this study, 962 cloacal swabs were collected from domestic ducks at several live poultry markets (LPMs) of Gilan, Mazandaran and Golestan provinces of Iran in the year 2017. Preliminary assays such as HI, NI, MDT, ICPI and RT-qPCR suggested that 0.5% of the birds were infected by H3 low pathogenic influenza viruses (LPAI). Three isolates were selected for whole genome sequencing. The cleavage site of the HA genes showed a PEKQTR/GLF motif, an indicator of LPAI. Furthermore, BLAST and phylogenetic analyses of the HA gene showed high homology to the Eurasian lineage of H3N8 AIV (95.5–97.1% to several European and E...

Bulgarian Journal of Veterinary Medicine

Shiga toxin-producing Escherichia coli (STEC) is recognised as toxin producing group of E. coli a... more Shiga toxin-producing Escherichia coli (STEC) is recognised as toxin producing group of E. coli and one of the most significant foodborne pathogens worldwide. The aim of this study was to detect STEC and determine virulence gene profiles of these pathogens in different kinds of meat and products in Iran. For this reason a total of 182 samples of minced beef, mutton, chicken meat, chicken feet and mechanically separated chicken meat were collected from retail markets for detection of STEC by PCR method. Of the 72 E. coli isolated from examined samples, 29 of them were STEC. The highest presence of STEC was detected in minced beef (23.5%) followed by chicken feet (15%), mutton (13.3%), mechanically separated chicken meat (12.5%) and chicken meat (5.5%) respectively. In addition the results of PCR assay indicated that 21 (72.4%) and 4 (13.7%) of isolates carried stx2 and eaeA genes respectively. However, according to the results stx2 was the most prevalent gene detected in all kinds of examined samples. Our findings showed that evaluation of the prevalence and virulence factors of this pathogen seems necessary considering the increasing importance of STEC as one of the most significant foodborne pathogens.

International Journal of Enteric Pathogens

Background: Avian pathogenic Escherichia coli (APEC) causes economic losses in the chicken indust... more Background: Avian pathogenic Escherichia coli (APEC) causes economic losses in the chicken industry worldwide. Objective: In this study, virulence-associated gene profiles of APEC isolates were investigated by polymerase chain reaction (PCR). Materials and Methods: A total of 60 Escherichia coli isolates were collected from 60 colibacillosis cases from 30 broiler poultry farms in Alborz, Tehran, and Golestan provinces, Iran. After identification by biochemical tests, DNA was extracted by boiling method and 5 virulence-associated genes including: iutA, hlyF, iroN, ompT, and iss were detected by 2 multiplex PCR protocols. Results: Of the 60 APEC isolates, 26 (43.3%) isolates had at least three virulence genes from which 12 (20%) isolates were positive for all 5 virulence genes, whereas 34 (56.6%) carried no investigated virulence genes. Presence of iutA, hlyF, iroN, ompT, and iss genes in the APEC isolates were 17 (28.3%), 17 (28.3%), 24 (40%), 26 (43.3%), and 23 (38.3%), respectively...

International Journal of Enteric Pathogens, 2016

Introduction: Diarrhea is the most common cause of death in neonatal calves. The most important a... more Introduction: Diarrhea is the most common cause of death in neonatal calves. The most important agents of diarrhea in young calves include bacteria, viruses, and protozoa. Only limited attention has been paid to the role of fungi in calves' diarrhea. Case Presentation: We report on a neonatal calf with fungal diarrhea caused by Candida albicans. The calf has had dysentery in the previous 10 days despite good appetite. The calf was then treated with oxytetracycline tabulations for 5 days. Conclusions: Yeasts and molds are sometimes associated with lesions in the stomach or intestines of scouring calves, but there is very limited information about their role in calf diarrhea. In this study, C. albicans was isolated in a 15-day-old dysenteric calf. These organisms are not a primary cause of diarrhea in calves, but like in children, they are possibly opportunistic pathogens that proliferate and invade the intestinal mucosa following antibiotic therapy.