yusuf Nergiz | Dicle University (original) (raw)

Papers by yusuf Nergiz

The Journal of reproductive medicine

Infertility is described as the inability of a couple to conceive after 1 year of unprotected int... more Infertility is described as the inability of a couple to conceive after 1 year of unprotected intercourse. Male factor is assumed to be responsible for 50% of cases of infertile couples. It is a common clinical problem, affecting approximately 13-15% of couples worldwide. In this study we aimed to investigate the effects of hypoosmotic swelling (HOS) and anti-HOS tests on sperm membrane integrity at the ultrastructural level. Twenty-nine infertile and 10 fertile men were included in this study. The fertile and infertile subjects were classified according to Kruger and WHO criterion. All semen samples were examined and ana-yzed based on WHO guidelines. Sperm viability was determined by using the eosin Y staining method. After HOS and anti-HOS tests wore applied, the samples were evaluated at the ultrastructural level. Normal structural features of all regions of sperm were observed in sections of sham normospermia. Some histopathological changes wore seen in HOS and anti-HOS group se...

International journal of health services research and policy, Apr 1, 2019

The placenta plays a role in the pathophysiology of preeclampsia. Preeclampsia is more common in ... more The placenta plays a role in the pathophysiology of preeclampsia. Preeclampsia is more common in multifetal pregnancies than singleton pregnancies. In this study, we aimed to investigate the histopathology of normotensive and preeclamptic placentas and the localization of alkaline phosphatase activity. In our study, 10 normotensive and 10 preeclamptic, totally 20 placentas were obtained.Paraffin sections were stained with Hematoxylin-Eosin, Masson trichrome and PAS for histopathological examination. Remaining sections were then stained via Gomori's method and micrographed under light microscope. Sections of the control group observed normal histologically structure. The alkaline phosphatase reaction was evident in the inner and outer membranes of the syncytiotrophoblasts. There was a significant increase in the number of syncytial knots, terminal villi and syncytial bridges in the preeclamptic placenta sections. Marked thickening of the trophoblast basal membranes were observed. Alkaline phosphatase reaction in preeclampsia group: The localization of alkaline phosphatase in the inner and outer membranes of the syncytiotrophoblasts was reduced. In placentas of preeclampsia group, a significant increase in syncytial knot, syncytial bridge were observed. The level of alkaline phosphatase enzyme in preeclamptic placentas was found to be lower compared to normotensive placenta.

Turgut Özal Tıp Merkezi Dergisi, 1998

The purpose of the present study was to investigate structural changes of the femur ofiactating a... more The purpose of the present study was to investigate structural changes of the femur ofiactating and postlactation periods fed low-caicium diet in rats. Twenty pregnancy Wistar albino rats weighing between 200-250 g were used for this study.The laboratory animals divided into two groups consist of 10 female rats.The experimental group was fed with low-calcium diet for 90 days (0.083 % Ca ).The puppy rats in this group following lactation and postlactation periods were fed with same diet. The control group was fed with standard diet (Standard pellet-0.5d%Ca). At the end of the experimental feeding period, the animals were sacrified and their femurs, removed enbloc sections (5mm) were taken and stained (Hematoxylen- Eosin, Masson Trichrome, Safranin fast green) for the light microscope. Light microscope examination; in the puppy rats in low-calcium diet group some histological sections of bone showed deminerilization area, an irregularitiy of inner surface of the lamellae and increase of the number of osteoclasts. Reducing the th ckness of bone cortex and spong osae trabeculae result from resorbt on were noted1 Key words: Low-calcium diet, femur, lactation, postlactation period, rat

Background: The aim of this study was to compare of term placentas, which has gestational diabete... more Background: The aim of this study was to compare of term placentas, which has gestational diabetes mellitus and normal pregnant women in terms of immunolocalization of MMP-2 and MMP-9. Methods: Fifteen gestational diabetes mellitus patients and fifteen women without any systemic disease of the placenta were collected. Fetal and maternal faces of the placentas, as well as peripheral and central parts were examined for immunohistochemical investigation of MMP-2 and MMP-9.Groups were evaluated through use of the SPSS 15.0 package program. P values ≤0.05 were considered statistically significant. Results: MMP-2 reaction was weakly expressed in maternal and fetal surface, and no significant difference between the two groups was observed. When MMP-9 expression was compared between the groups, an increase in decidual cells of diabetic group and a decrease in syncytial nodes were noticed. In fetal surface, a decrease in expression level was detected in chorionic villus syncytiotrophoblasts, chorionic villus stroma, stem villus syncytiotrophoblasts, stem villous stroma and chorionic plate. Conclusion: Regarding our findings, MMP-9 plays a more active role than MMP-2 in gestational diabetic placentas was concluded.

Dicle Tıp Dergisi, Dec 1, 2008

Calismamizda, bir statin turevi olan atorvastatinin sican iskelet kasinda olusturdugu yapisal deg... more Calismamizda, bir statin turevi olan atorvastatinin sican iskelet kasinda olusturdugu yapisal degisikliklerinisik mikroskobu duzeyinde incelenmesi ve degerlendirilmesi amaclandi.40 adet eriskin erkek wistar albinorat; biri kontrol ve dokuzu deney gruplari olmak uzere 10 esit gruba bolundu. . Deneyin baslamasindansonra 5. 10. ve 15. gunlerde sakrifiye edilen ratlarin quadriceps femoris kaslari isik mikroskobundaincelendi. Kontrol grubundan elde edilen kaslarin histopatolojik incelemelerinde herhangi bir yapisaldegisiklige rastlanmazken, diger 9 deney grubundan elde edilen kas orneklerinde degisen derecelerdehistopatolojik bulgular elde edildi. Genel olarak kaslarda dejenerasyon, kontraksiyon, nekroz, bagdokuartisi, mononukleer hucre infiltrasyonu, kas lifleri arasinda vakuoler yapilar ve sentral nukleus belirgin artisgostermekteydi. Calismamizda bulgularimiz, doz ve zaman arttikca degisikliklerin belirgin oldugugozlendi. Atorvastatinin iskelet kaslarinda gostermis oldugu toksik etki ile birlikte, klinik acidan doz vezaman baz alinarak, hasta tedavilerine isik tutacagi kanaatine varildi.

DergiPark (Istanbul University), Mar 1, 2000

The increasing use of wireless communication devices has raised major concerns towards deleteriou... more The increasing use of wireless communication devices has raised major concerns towards deleterious effects of microwave radiation on human health. The aim of the study was to demonstrate the effect of low-intensity microwave radiation on levels of monoamine neurotransmitters and gene expression of their key regulating enzymes in brain of Fischer rats. Animals were exposed to 900 MHz and 1800 MHz microwave radiation for 30 days (2 h/day, 5 days/week) with respective specific absorption rates as 5.953 9 10-4 and 5.835 9 10-4 W/kg. The levels of monoamine neurotransmitters viz. dopamine (DA), norepinephrine (NE), epinephrine (E) and serotonin (5-HT) were detected using LC-MS/MS in hippocampus of all experimental animals. In addition, mRNA expression of key regulating enzymes for these neurotransmitters viz. tyrosine hydroxylase (TH) (for DA, NE and E) and tryptophan hydroxylase (TPH1 and TPH2) (for serotonin) was also estimated. Results showed significant reduction in levels of DA, NE, E and 5-HT in hippocampus of microwave-exposed animals in comparison with sham-exposed (control) animals. In addition, significant downregulation in mRNA expression of TH, TPH1 and TPH2 was also observed in microwave-exposed animals (p \ 0.05). In conclusion, the results indicate that low-intensity microwave radiation may cause learning and memory disturbances by altering levels of brain monoamine neurotransmitters at mRNA and protein levels.

Regional Anesthesia and Pain Medicine, Sep 1, 2004

European review for medical and pharmacological sciences, 2014

OBJECTIVE The present study aimed to investigate the effects of the bacterial biofilm formation o... more OBJECTIVE The present study aimed to investigate the effects of the bacterial biofilm formation on the tonsil surface exposed N-acetyl-cysteine (NAC) and acetylsalicylic acid (ASA) of patients undergoing tonsillectomy by light and electron microscopy. The general process of biofilm formation comprises adhesion of free-living or planktonic bacteria to a surface, which subsequently develop into microcolonies and form a biofilm. Based on studies that have shown the presence of biofilms in common sites of chronic infections, it has become clear that bacteria may persist on mucosal surfaces through formation of biofilms. PATIENTS AND METHODS Ten patients between 4 and 39 years of age (mean, 11.9 ± 11.2 years). In all cases, periodic acide Schiff (PAS) staining was found to be an accurate predictor of the presence or absence of biofilm using light microscopy as a control standard. Therapeutic doses of NAC and ASA were identificated as the effective on the tonsil bacterial biofilm using li...

Journal of Pediatric Urology, Aug 1, 2020

This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

10th European Congress of Endocrinology, May 1, 2008

After the complete removal of submandibular gland surgically, morphological alterations, and the ... more After the complete removal of submandibular gland surgically, morphological alterations, and the effects of EGF given orally on these alterations were investigated. In the study, 30 adult female SpraqueDawley rats were used, which were divided equally into three groups: Control group; sialoadenectomy group ; sialoadenectomy + epidermal growth factor group . Following a three week period of recovery, the rats were mated. On days 16-19 th of pregnancy, a total of 5 microgram EGF was given to the animals in sialoadenectomy + epidermal growth factor group with orogastric tube as 1.25µg daily to each animal. The fetuses born following pregnancy were kept to grow up until the 28 th day . The pupy rat of 28 days in all groups were taken, and then they were sacrificed. Tongues of all puppy rats were fixed in a 10 % neutral buffered formalin solution. The paraffin sections obtained through routine histological methods were stained with Hematoxylen–Eosine , Methylen blue- Basic Fuchsin, Hemotoxylen–Van Giesson, and were examined under light microscope. A significant increase in the keratinization; an elongation and bifurcation at the ends of the filiform papillae of the sialoadenectomy group rats were observed . The fungiform papilla and the taste buds were noticed to be larger than that of the control group, and a decrease was detected in the cells population that constitutes the taste buds. Bifurcation and hyperkeratosis in the filiform papillae of the sialoadenectomy + epidermal growth factor group rats.The morphological appearance of fungiform papillae and taste buds was observed to be similar to that of fungiform papillae of the control group. (Journal of International Dental and Medical Research 2009; 2: (3), pp. 100-104)

African Journal of Biotechnology, Aug 23, 2010

This study was undertaken to investigate the effect of valproic acid and protective effects of vi... more This study was undertaken to investigate the effect of valproic acid and protective effects of vitamin E and folic acid on rat ovary ultrastructural changes. Twenty-four Wistar rats were used. Animals were divided into four groups. The first group of rats was used as control. The second group was injected valproic acid. The third group was injected valproic acid + folic acid and the fourth group was given valproic acid + vitamin E. At the end of the study, ovarium tissues were taken under anesthesia. Tissues were prepared and examined by transmission electron microscopy. Microscopically, the groups are cited as follows: Control group (in which the ovarium tissue was normal), valproic acid group (which showed increase in lipid content plus mitochondrial crystalysis seen in folliculer and theca interna cells of rat ovarium), valproic acid + folic acid group (in which the theca interna and granulosa cells of rat ovarium had normal appearance) and valproic acid + vitamin E group (where all the organelles of theca interna and granulosa cells of rat ovarium were observed to be normal). Vitamin E and folic acid have protective effects against valproic acid-induced tissue damage in rat ovaries.

Journal of International Dental and Medical Research, Sep 1, 2010

Gestational choriocarcinoma is a higly malignant epithelial tumour arising from the trophoblasts ... more Gestational choriocarcinoma is a higly malignant epithelial tumour arising from the trophoblasts of any type of gestational event, most often a hydatidiform mole . Choriocarcinoma is for all practical purposes limited to reproductive age women but rare examples of choriocarcinoma in postmenoupausal women have been reported. In case of a 24- years- old was a female patient with left hemiparalysis. Biopsy that obtained from lower gingival ulcer indicated the availability of choriocarcinoma metastasis. Before putting

Biotechnology & Biotechnological Equipment, 2003

... ULTRASTRUCTURAL CHANGES IN RAT HEPATOCYTES AFTER PROLONGED FASTING UYBilgin,Y.Nergiz Dicle Un... more ... ULTRASTRUCTURAL CHANGES IN RAT HEPATOCYTES AFTER PROLONGED FASTING UYBilgin,Y.Nergiz Dicle University, Faculty of Medicine, Department of Histology and Embryology, 21280 Diyarbakýr,Turkey include ...

Glutamatergic N-methyl-D-aspartate (NMDA) receptors have vital roles in memory formation. Changes... more Glutamatergic N-methyl-D-aspartate (NMDA) receptors have vital roles in memory formation. Changes in the activity of these receptors can influence memory processes. Ketamine is a non-competitive NMDA receptor antagonist drug with promising mood-altering and pain-reducing effects in low doses. The present study investigated the effects of low-dose ketamine administrations on memory acquisition, consolidation, and retrieval processes. Sixty-four male BALB/c mice used in this experiment were treated with a selective serotonin reuptake inhibitor, fluoxetine, and a serotonin depletion agent, p-chlorophenylalanine (pCPA). A serotonin antagonist, methiothepin, and ketamine were administered 60 mins before or after the behavioral tests. A passive avoidance test measured emotional memory acquisition, consolidation, and retrieval processes. Hippocampi malondialdehyde (MDA) levels were analyzed, and histopathological examinations were performed. Ketamine alone did not significantly affect memo...

Iranian journal of kidney diseases, 2017

Hyperbaric oxygen (HBO) treatment is steadily increasing as a therapeutic modality for various ty... more Hyperbaric oxygen (HBO) treatment is steadily increasing as a therapeutic modality for various types of diseases. Although good clinical outcomes were reported with HBO treatment for various diseases, the multisystemic effects of this modality are still unclear. This study aimed to investigate the renal effects of HBO experimentally. Fourteen New Zealand White rabbits were divided into 2 groups randomly as the control group and the study group. The study group received HBO treatment for 28 days (100% oxygen at 2.5 atmospheres for 90 minutes daily) and the control group was used to obtain normal renal tissue of the animal genus. After the intervention period, venous blood samples were obtained, and renal tissue samples were harvested for comparisons. Normal histological morphology was determined with Masson trichrome staining and periodic acid-Schiff staining in the control group. Atrophic glomerular structures, vacuolated tubule cells, and degeneration were detected in the renal sam...

Cell Biochemistry and Biophysics, 2015

International Journal of Morphology, Mar 1, 2011

In this study, we evaluated the ultrastructural findings of testis with systemic administration o... more In this study, we evaluated the ultrastructural findings of testis with systemic administration of different doses of melatonin during ischemic period in a rat model of testicular torsion/detorsion (T/D). Testis ischemia-reperfusion (I/R) injury was induced by torsion of the left testis, with a 720 degrees twisting of the spermatic cord so as to produce a total occlusion of testis for 2.5 hours. Subsequently, the same testis was then detorsioned. According to surgical procedure in each group, unilateral orchiectomies were performed for histopathologic examination. The groups were labelled as control group, torsion group (T), torsion and detorsion group (T/ D), torsion-detorsion and melatonin group (T/D+20,50 and 100 mg/kg melatonin). For the histological examination, testicular tissues were fixed in 2.5% glutheraldehyde and postfixation 1% osmic acid solutions. They were examined under transmission electron microscopy after application of contrast stained. In torsion group testis cross-sections, cytoplasm residues of mature sperms and large vacuole-like structures were noticeable. In detorsion group testis cross-sections, dissociations in spermatocide nuclei, many vacuoles and residual particles resulting from organelle degeneration, local voids in cytoplasms of spermatogonia, dilatation in granulated endoplasmic reticulum, large lipid droplets, chromatid particles, along with mitochondrial crystalisis were determined. In the testis cross-sections of the group of T/D+50 mg/kg melatonin administration, sertoli and spermatogonia cells that showed membrane-like structures and cytoplasmic voids were observed. Testis cross-sections of rats that were administered with T/D+50 mg/kg melatonin showed small mitochondrions and vacuole-like structures placed on the edge. Testis cross-sections of rats that were administered with T/D+100 mg/kg melatonin resulted in views similar to those of controls in the microstructural level. As a result, the most effective dose of melatonin, which was used in different doses, for prevention of ischemia/reperfusion damage was found to be 100 mg/kg.

Dicle Tıp Dergisi, 2007

... 233 Göbek Kordonu Kan ve Stromal Kökenli Hücrelerin Sinir Hücrelerine Farklılaşması Özlem Pam... more ... 233 Göbek Kordonu Kan ve Stromal Kökenli Hücrelerin Sinir Hücrelerine Farklılaşması Özlem Pamukçu Baran, Yusuf Nergiz, Selen Bahçeci ... Nöral kök hücreler immatürdür, hem gelişmekte olan beyinde hem de erişkin sinir sisteminde bulunan bağımsız hücrelerdir. ...