Nucleic Acid Detection of Live Pathogens on Contaminated Foods (original) (raw)

Abstract

The goal is to develop a point-of-care biosensor for the detection of live pathogens contaminating beef products. Biosensing of live pathogens is based on isothermal amplification of nucleic acid on a paper-based device. A colorimetric dye is employed as an indicator of the amplification product for visual result. The assay incorporates a compound Propidium monoazide (PMA) that makes the DNA from dead cells inaccessible for amplification. This approach is especially applicable for pathogens that can enter a viable but non-culturable state (VBNC).

Start Date

2-3-2023 3:00 PM

Kaur, Simerdeep and Verma, Mohit, "Nucleic Acid Detection of Live Pathogens on Contaminated Foods" (2023). Graduate Industrial Research Symposium. 16.
https://docs.lib.purdue.edu/girs/2023/posters/16

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Nucleic Acid Detection of Live Pathogens on Contaminated Foods

The goal is to develop a point-of-care biosensor for the detection of live pathogens contaminating beef products. Biosensing of live pathogens is based on isothermal amplification of nucleic acid on a paper-based device. A colorimetric dye is employed as an indicator of the amplification product for visual result. The assay incorporates a compound Propidium monoazide (PMA) that makes the DNA from dead cells inaccessible for amplification. This approach is especially applicable for pathogens that can enter a viable but non-culturable state (VBNC).