A Protein Interaction Map of the Mitotic Spindle (original) (raw)

• Jonathan Wong, University of California, Berkeley
• Yuko Nakajima, University of California, Berkeley
• Stefan Westermann, Research Institute of Molecular Pathology
• Ching Shang, University of California, Berkeley
• Jung-Seog Kang, The University of Texas, Austin
• Crystal Goodner, University of Washington
• Pantea Houshmand, University of California, Berkeley
• Stanley Fields, University of Washington
• Clarence S.M. Chan, The University of Texas, Austin
• David Drubin, University of California, Berkeley
• Georjana Barnes, University of California, Berkeley
• Tony R. Hazbun, Purdue UniersityFollow

Abstract

The mitotic spindle consists of a complex network of proteins that segregates chromosomes in eukaryotes. To strengthen our understanding of the molecular composition, organization, and regulation of the mitotic spindle, we performed a system-wide two-hybrid screen on 94 proteins implicated in spindle function in Saccharomyces cerevisiae. We report 604 predominantly novel interactions that were detected in multiple screens, involving 303 distinct prey proteins. We uncovered a pattern of extensive interactions between spindle proteins reflecting the intricate organization of the spindle. Furthermore, we observed novel connections between kinetochore complexes and chromatin-modifying proteins and used phosphorylation site mutants of NDC80/TID3 to gain insights into possible phospho-regulation mechanisms. We also present analyses of She1p, a novel spindle protein that interacts with the Dam1 kinetochore/spindle complex. The wealth of protein interactions presented here highlights the extent to which mitotic spindle protein functions and regulation are integrated with each other and with other cellular activities.

Date of this Version

7-18-2007

DOI

10.1091/mbc.e07-06-0536

Recommended Citation

Wong J, Nakajima Y, Westermann S, Shang C, Kang JS, Goodner C, Houshmand P, Fields S, Chan CS, Drubin D, Barnes G, Hazbun T. A protein interaction map of the mitotic spindle. Mol Biol Cell. 2007 Oct;18(10):3800-9. doi: 10.1091/mbc.e07-06-0536. Epub 2007 Jul 18. PMID: 17634282; PMCID: PMC1995735.

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