Segmented filamentous bacteria in a defined bacterial cocktail induce intestinal inflammation in SCID mice reconstituted with CD45RBhigh CD4+ T cells (original) (raw)

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1Laboratory of Gnotobiology, Department of Immunology, Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czech Republic

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2Nuffield Department of Surgery, University of Oxford, John Radcliffe Hospital, Oxford, UK

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3Laboratory of Electron Microscopy, Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic

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1Laboratory of Gnotobiology, Department of Immunology, Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czech Republic

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1Laboratory of Gnotobiology, Department of Immunology, Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czech Republic

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1Laboratory of Gnotobiology, Department of Immunology, Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czech Republic

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2Nuffield Department of Surgery, University of Oxford, John Radcliffe Hospital, Oxford, UK

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2Nuffield Department of Surgery, University of Oxford, John Radcliffe Hospital, Oxford, UK

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1Laboratory of Gnotobiology, Department of Immunology, Institute of Microbiology, Czech Academy of Sciences, Novy Hradek, Czech Republic

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3Laboratory of Electron Microscopy, Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic

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Received:

07 December 2006

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Renata Stepankova, Fiona Powrie, Olga Kofronova, Hana Kozakova, Tomas Hudcovic, Tomas Hrncir, Holm Uhlig, Simon Read, Zuzana Rehakova, Oldrich Benada, Pioter Heczko, Magda Strus, Paul Bland, Helena Tlaskalova-Hogenova, Segmented filamentous bacteria in a defined bacterial cocktail induce intestinal inflammation in SCID mice reconstituted with CD45RBhigh CD4+ T cells, Inflammatory Bowel Diseases, Volume 13, Issue 10, 1 October 2007, Pages 1202–1211, https://doi.org/10.1002/ibd.20221
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Abstract

Background

The aim was to analyze the influence of intestinal microbiota on the development of intestinal inflammation. We used the model of chronic inflammation that develops spontaneously in the colon of conventional severe combined immunodeficiency (SCID) mice restored with the CD45 RBhigh subset of CD4+T cells isolated from the spleen of normal BALB/c mice.

Methods

A CD4+CD45RBhigh subpopulation of T cells was purified from the spleen of conventional BALB/c mice by magnetic separation (MACS) and transferred into immunodeficient SCID mice. Germ-free (GF) SCID mice or SCID mice monoassociated with Enterococcus faecalis, SFB (segmented filamentous bacteria), Fusobacterium mortiferum, Bacteroides distasonis, and in combination Fusobacterium mortiferum + SFB or Bacteroides distasonis + SFB were used as recipients. SCID mice were colonized by a defined cocktail of specific pathogen-free (SPF) bacteria. Mice were evaluated 8–12 weeks after the cell transfer for clinical and morphological signs of inflammatory bowel disease (IBD).

Results

After the transfer of the CD4+CD45RBhigh T-cell subpopulation to SCID mice severe colitis was present in conventional animals and in mice colonized with a cocktail of SPF microflora plus SFB. Altered intestinal barrier in the terminal ileum of mice with severe colitis was documented by immunohistology using antibodies to ZO-1 (zona occludens).

Conclusions

Only SFB bacteria together with a defined SPF mixture were effective in triggering intestinal inflammation in the model of IBD in reconstituted SCID mice, while no colitis was detected in GF mice or in mice colonized either with SPF microflora or monoassociated only with SFB or colonized by Bacteroides distasonis + SFB or Fusobacterium mortiferum + SFB.

Copyright © 2007 Crohn's & Colitis Foundation of America, Inc.

Topic:

• inflammation
• inflammatory bowel disease
• bacteroides
• colitis
• intestines
• scid mice
• t-lymphocytes
• bacteria
• colon
• mice
• distal ileum
• microbial colonization
• transfer technique

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