Twin Studies Reveal Specific Imbalances in the Mucosaassociated Microbiota of Patients with Ileal Crohn's Disease (original) (raw)

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1Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden

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2Department of Medicine, Division of Gastroenterology, Örebro University Hospital, Örebro, Sweden

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1Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden

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1Department of Microbiology, Swedish University of Agricultural Sciences, Uppsala, Sweden

3Department of Oncology, Radiology and Clinical Immunology, Uppsala University Hospital, Uppsala, Sweden

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2Department of Medicine, Division of Gastroenterology, Örebro University Hospital, Örebro, Sweden

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4Department of Bacteriology, Swedish Institute for Infectious Disease Control, Solna, Sweden

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2Department of Medicine, Division of Gastroenterology, Örebro University Hospital, Örebro, Sweden

5School of Health and Medical Sciences, Örebro University, Örebro, Sweden

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6Lawrence Berkeley National Laboratory, Division of Earth Sciences, Berkeley, California

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Accepted:

09 September 2008

Published:

20 November 2008

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Ben Willing, Jonas Halfvarson, Johan Dicksved, Magnus Rosenquist, Gunnar Järnerot, Lars Engstrand, Curt Tysk, Janet K. Jansson, Twin Studies Reveal Specific Imbalances in the Mucosaassociated Microbiota of Patients with Ileal Crohn's Disease, Inflammatory Bowel Diseases, Volume 15, Issue 5, 1 May 2009, Pages 653–660, https://doi.org/10.1002/ibd.20783
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Background

Large interindividual variation in the composition of the intestinal microbiota between unrelated individuals has made it challenging to identify specific aspects of dysbiosis that lead to Crohn's disease (CD).

Methods

To reduce variations in exposure during establishment of the gut flora and the influence of genotype, we studied the mucosa-associated microbiota of monozygotic twin pairs that were discordant (n = 6) or concordant (n = 4) for CD. DNA was extracted from biopsies collected from 5 locations between the ileum and rectum. Bacterial 16S ribosomal RNA genes were amplified and community composition assessed by terminal-restriction fragment length polymorphism, cloning and sequencing, and quantitative real-time polymerase chain reaction (PCR).

Results

The microbial compositions at all biopsy locations for each individual were similar, regardless of disease state, but there were differences between individuals. In particular, individuals with predominantly ileal CD had a dramatically lower abundance (P < 0.001) of Faecalibacterium prausnitzii and increased abundance (P < 0.03) of Escherichia coli compared to healthy co-twins and those with CD localized in the colon. This dysbiosis was significantly correlated to the disease phenotype rather than genotype.

Conclusions

The reduced abundance of F. prausnitzii and increased abundance of E. coli are indicative of an ileal CD phenotype, distinct from colonic CD, and the relative abundances of these specific bacterial populations are promising biomarker candidates for differential diagnosis of CD and eventually customized treatment.

Copyright © 2009 Crohn's & Colitis Foundation of America, Inc.

Topic:

• phenotype
• polymerase chain reaction
• polymorphism
• biopsy
• crohn's disease
• cloning
• biological markers
• dna
• genes
• genotype
• intestines
• rna, ribosomal, 16s
• twins
• monozygotic twins
• colon
• ileum
• mucous membrane
• rectum
• escherichia coli
• intestinal bacteria
• community
• microbiome
• quantitative real-time polymerase chain reaction
• dysbiosis
• faecalibacterium prausnitzii

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