Tetrachloroethylene, trichloroethylene, and chlorinated phenols induce toluene-o-xylene monooxygenase activity in Pseudomonas stutzeri OX1 (original) (raw)

Abstract.

Pseudomonas stutzeri OX1 naphthalene-oxidation activity is induced 3.0-fold by tetrachloroethylene (PCE) and 3.1-fold by trichloroethylene (TCE) at 100 µM. With the mutant P. stutzeri M1, which does not express toluene-_o-_xylene monooxygenase (ToMO, product of the tou operon), no naphthalene-oxidation activity induction by PCE and TCE was found; hence, PCE and TCE induce ToMO of P. stutzeri OX1. The chlorinated phenols 2-, 3-, and 4-chlorophenol induced ToMO expression 0.58-, 0.23- and 0.37-fold, respectively, compared to the direct inducer of the pathway, _o-_cresol. Using P. putida PaW340 (pPP4062, pFP3028), which has the tou promoter fused to the reporter catechol-2,3-dioxygenase, and the regulator gene touR, it was determined that the tou promoter was induced directly 5.7-, 7.1-, and 5.1-fold for 2-, 3-, and 4-chlorophenol, respectively (compared to an 8.8-fold induction with _o-_cresol). In addition, it was found that TCE and PCE do not directly induce the tou pathway and that components other than the tou structural and regulatory genes are necessary for induction. Gas chromatography results also showed that 100 µM TCE induced its own degradation (8–9%) in 16 h in P. stutzeri OX1, and all of the stoichiometric chloride from the degraded TCE was detected in solution.

Access this article

Log in via an institution

Subscribe and save

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Chemical Engineering, University of Connecticut, Storrs, CT 06269–3222, USA, , , , ,
    D. Ryoo, H. Shim & T. Wood
  2. Dipartimento di Genetica e di Biologia dei Microrganismi Universita' degli studi di Milano, 20133 Milan, Italy, , , , ,
    F. Arenghi & P. Barbieri

Authors

  1. D. Ryoo
    You can also search for this author inPubMed Google Scholar
  2. H. Shim
    You can also search for this author inPubMed Google Scholar
  3. F. Arenghi
    You can also search for this author inPubMed Google Scholar
  4. P. Barbieri
    You can also search for this author inPubMed Google Scholar
  5. T. Wood
    You can also search for this author inPubMed Google Scholar

Additional information

Received revision: 8 March 2001

Electronic Publication

Rights and permissions

About this article

Cite this article

Ryoo, .D., Shim, .H., Arenghi, .F. et al. Tetrachloroethylene, trichloroethylene, and chlorinated phenols induce toluene-_o_-xylene monooxygenase activity in Pseudomonas stutzeri OX1.Appl Microbiol Biotechnol 56, 545–549 (2001). https://doi.org/10.1007/s002530100675

Download citation

Keywords