Inactivation of Paragonimus westermani metacercariae in soy sauce-marinated and frozen freshwater crabs (original) (raw)
Abstract
Soy sauce-marinated freshwater crabs (Eriocheir japonicus) are a source of human paragonimiasis. The viability of Paragonimus westermani metacercariae (PwMc) in marinated crabs was investigated in an experimental setting. The PwMc collected from freshwater crayfish were inoculated into freshwater crabs, which were then frozen or marinated in soy sauce. All PwMc in the freshwater crabs were inactivated after freezing for 48 h at −20 °C and after freezing for 12 h at −40 °C. After marinating for 32 days, the survival rate of PwMc in 5% NaCl soy sauce was 50%, in 7.5% NaCl soy sauce it was 33.3%, and in 10.0% NaCl soy sauce it was 31.3%. When marinated for 64 days, all PwMc were inactivated in all experimental groups. These results revealed that freezing and soy sauce marination were detrimental to the survival of PwMc in freshwater crabs. Specifically, freezing crabs for more than 48 h or soaking them in soy sauce containing at least 5.0% NaCl for 64 days can inactivate PwMc. These results can inform the production of the traditional Korean soy sauce-marinated freshwater crabs known as gejang.
Access this article
Subscribe and save
- Starting from 10 chapters or articles per month
- Access and download chapters and articles from more than 300k books and 2,500 journals
- Cancel anytime View plans
Buy Now
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Instant access to the full article PDF.
Similar content being viewed by others
References
- Blair D, Xu ZB, Agatsuma T (1999) Paragonimiasis and the genus Paragonimus. Adv Parasitol 42:113–222
Article CAS PubMed Google Scholar - Chai JY (2013) Paragonimiasis. Handb Clin Neurol 114:283–296
Article PubMed Google Scholar - Cho SY, Kang SY, Kong Y, Yang HJ (1991) Metacercarial infections of Paragonimus westermani in freshwater crabs sold in markets in Seoul. Korean J Parasitol 29:189–191
Article CAS Google Scholar - Cho AR, Lee HR, Lee KS, Lee SE, Lee SY (2011) A case of pulmonary paragonimiasis with involvement of the abdominal muscle in a 9-year-old girl. Korean J Parasitol 49:409–412
Article PubMed PubMed Central Google Scholar - Fan PC (1998) Viability of metacercariae of Clonorchis sinensis in frozen or salted freshwater fish. Int J Parasitol 28:603–605
Article CAS PubMed Google Scholar - Food and Drug Administration, USA (2001) Food code, section 3-402.11 (A1 and A2). http://www.fda.gov/Food/GuidanceRegulation/RetailFoodProtection/FoodCode/ucm2016794.htm
- Kang SY, Kim TK, Kim TY, Ha YI, Choi SW, Hong SJ (2000) A case of chronic cerebral paragonimiasis westermani. Korean J Parasitol 38:167–171
Article CAS PubMed PubMed Central Google Scholar - Keiser J, Utzinger J (2007) Food-borne trematodiasis: current chemotherapy and advances with artemisinins and synthetic trioxolanes. Trends Parasitol 23:555–562
Article CAS PubMed Google Scholar - Kim EM, Kim JL, Choi SI, Lee SH, Hong ST (2009) Infection status of freshwater crabs and crayfish with metacercariae of Paragonimus wertermani in Korea. Korean J Parasitol 47:425–426
Article PubMed PubMed Central Google Scholar - Koh EJ, Kim SK, Wang KC, Chai JY, Chong S, Park SH, Cheon JE, Phi JH (2012) The return of an old worm: cerebral paragonimiasis presenting with intracerebral hemorrhage. J Korean Med Sci 27:1428–1432
Article PubMed PubMed Central Google Scholar - Lee CH, Kim JH, Moon WS, Lee MR (2012) Paragonimiasis in the abdominal cavity and subcutaneous tissue: report of 3 cases. Korean J Parasitol 50:345–347
Article PubMed PubMed Central Google Scholar - Sim YS, Lee JH, Hong SC, Chang JH, Kang SR, Yang HJ, Sung SH (2010) Paragonimus westermani found in the tip of a little finger. Intern Med 49:1645–1648
Article PubMed Google Scholar - Sanchez-Monsalvez I, Armas-Serra C, Martinez J, Dorado M, Sanchez A, Rodriguez-Caabeiro F (2005) A new procedure for marinating fresh anchovies and ensuring the rapid destruction of Anisakis laevae. J Food Prot 68:1066–1072
Article CAS PubMed Google Scholar - Sohn BS, Bae YJ, Cho YS, Moon HB, Kim TB (2009) Three cases of paragonimiasis in a family. Korean J Parasitol 47:281–285
Article PubMed PubMed Central Google Scholar - Wootten R, Cann DC (2001) Round worms in fish. In Torry advisory note no. 80. Ministry of Agriculture, Fisheries and Food. Torry Research Station. http://www.fao.org/wairdocs/tan/x5951e/x5951e00.htm
- World Health Organization (2002) Report: Joint WHO/FAO Workshop on Foodborne Trematode Infections in Asia, Hanoi, Vietnam. http://whqlibdoc.who.int/wpro/2004/RS_2002_GE_40(VTN).pdf
Acknowledgements
This research was supported by a 2012 grant (12162KFDA12) from the Korea Food and Drug Administration for studies on hazardous microbes and microbiological safety management of seafood.
Author information
Authors and Affiliations
- Department of Medical Environmental Biology, College of Medicine, Chung-Ang University, Dongjak-gu, Seoul, 06974, South Korea
Tae Im Kim, Fuhong Dai & Sung-Jong Hong - Department of Food Science and Technology, Chung-Ang University, 72-1 Naeri, Daedoek-myeon, Anseong-si, Gyeonggi-do, 17546, South Korea
Se-Ra Oh & Sang-Do Ha - Departments of Parasitology, Ewha Womans University School of Medicine, Yangcheon-gu, Seoul, 07985, South Korea
Hyun-Jong Yang
Authors
- Tae Im Kim
- Se-Ra Oh
- Fuhong Dai
- Hyun-Jong Yang
- Sang-Do Ha
- Sung-Jong Hong
Corresponding author
Correspondence toSung-Jong Hong.
Ethics declarations
Not applicable.
Rights and permissions
About this article
Cite this article
Kim, T.I., Oh, SR., Dai, F. et al. Inactivation of Paragonimus westermani metacercariae in soy sauce-marinated and frozen freshwater crabs.Parasitol Res 116, 1003–1006 (2017). https://doi.org/10.1007/s00436-017-5380-7
- Received: 14 July 2016
- Accepted: 16 January 2017
- Published: 26 January 2017
- Issue date: March 2017
- DOI: https://doi.org/10.1007/s00436-017-5380-7