Cathepsin K Expression in Human Lung (original) (raw)

Abstract

Tissue remodeling is crucial in different lung diseases, in the embryonal development as well as in bronchial carcinoma. Cathepsins were proposed to be involved in the degradation of matrix proteins. Cathepsin K is one of the most potent matrix-degrading cysteine proteinases known as yet. The elastinolytic and collagenolytic activity of this papain-like protease is comparable with that of neutrophil elastase. We have investigated the cathepsin K expression in normal adult lung tissues, in embryonal lung tissue and in bronchial carcinoma. With help of specific anti-cathepsin K antibodies it could be shown that cathepsin K was expressed in bonchial epithelial cells. These data could be confirmed at mRNA level using a quantitative RT-PCR as well as by visualisation of the specific enzymatic activity in epithelial cell lines. During the embryonal development cathepsin K was expressed in the epithelial cells of the developing bronchi. The expression seemed to be upregulated in parallel with the development of the bronchial and alveolar lumen. In the later phase of lung development the cathepsin K expression was restricted to bronchial epithelial cells. Furthermore, using quantitative RT- PCR it could be shown that cathepsin K-mRNA was upregulated in lung tumor tissues in comparison to normal tissues from the same patients. These data suggest that cathepsin K may play an important role in matrix remodeling of the lung under physiological and pathological conditions.

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Authors and Affiliations

1. Inst. oj Immunology, Otto-von-Guericke University Magdeburg, Magdeburg, Germany
   Frank Bühling & Nadine Waldburg
2. Dept. of Pneumology and Critical Care, Otto-von-Guericke University Magdeburg, Magdeburg, Germany
   Tobias Welte
3. Inst. Exptl. Int. Med., Otto-von-Guericke University Magdeburg, Magdeburg, Germany
   Dirk Reinhold
4. Inst. of Pathology, Otto-von-Guericke University Magdeburg, Magdeburg, Germany
   Carsten Häckel & Sabine Krüger
5. Dept. of Human Genetics, Ml. Sinai Medical School New York, USA
   Dieter Brömme
6. Inst. of Physiological Chemistry, Martin-Luther-University Halle, Germany
   Ekkehard Weber

Authors

1. Frank Bühling
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2. Nadine Waldburg
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3. Annegret Gerber
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4. Carsten Häckel
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5. Sabine Krüger
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6. Dirk Reinhold
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7. Dieter Brömme
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8. Ekkehard Weber
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9. Siegfried Ansorge
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10. Tobias Welte
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Editor information

Editors and Affiliations

1. Martin Luther University, Halle, Germany
   Jürgen Langner
2. Otto von Guericke University, Magdeburg, Germany
   Siegfried Ansorge

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© 2002 Kluwer Academic Publishers

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Bühling, F. et al. (2002). Cathepsin K Expression in Human Lung. In: Langner, J., Ansorge, S. (eds) Cellular Peptidases in Immune Functions and Diseases 2. Advances in Experimental Medicine and Biology, vol 477. Springer, Boston, MA. https://doi.org/10.1007/0-306-46826-3\_30

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• DOI: https://doi.org/10.1007/0-306-46826-3\_30
• Publisher Name: Springer, Boston, MA
• Print ISBN: 978-0-306-46383-9
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