Antioxidant Properties of Quercetin (original) (raw)

Abstract

Quercetin, a plant-derived aglycone form of flavonoid glycosides, has been used as a nutritional supplement and may be beneficial against a variety of diseases, including cancer. We examined the antioxidant properties of quercetin. The reduction potential of quercetin was measured at various pH values using voltammetric methods, and its total antioxidant capacity (TAC) was measured using the phosphomolybdenum method. The effect of quercetin on production of reactive oxygen species (ROS) and nitric oxide (NO) in LPS-stimulated human THP-1 acute monocytic leukemia cells was determined by flow cytometry using CM-H2DCFDA dye. The results were compared with curcumin, a natural product exhibiting a similar range of reported health benefits. Results: 1) Quercetin has a higher reduction potential compared with curcumin at three different pH settings and is comparable to Trolox at pH 7-9.5; 2) its TAC is 3.5 fold higher than curcumin; 3) it reduced LPS-induced ROS to near normal levels; 4) it reduced LPS-induced NO production. These data provide a physico-chemical basis for comparing antioxidants, with potential benefits individually or in combination.

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Authors and Affiliations

  1. Department of Radiation Oncology, University of Florida, 2033 Mowry Road, 103633, Gainesville, FL, 32610, USA
    Mei Zhang, Steven G. Swarts, Liangjie Yin, Steven B. Zhang, Lurong Zhang & Paul Okunieff
  2. Department of Radiation Oncology, University of Rochester Medical Center, 601 Elmwood Avenue, Rochester, NY, 14642, USA
    Chaomei Liu, Yeping Tian, Yongbing Cao, Michael Swarts, Shanmin Yang, Kunzhong Zhang, Shaoqing Ju, David J. Olek Jr., Lisa Schwartz, Peter C. Keng & Rob Howell

Authors

  1. Mei Zhang
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  2. Steven G. Swarts
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  3. Liangjie Yin
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  4. Chaomei Liu
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  5. Yeping Tian
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  6. Yongbing Cao
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  7. Michael Swarts
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  8. Shanmin Yang
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  9. Steven B. Zhang
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  10. Kunzhong Zhang
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  11. Shaoqing Ju
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  12. David J. Olek Jr.
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  13. Lisa Schwartz
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  14. Peter C. Keng
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  15. Rob Howell
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  16. Lurong Zhang
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  17. Paul Okunieff
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Corresponding author

Correspondence toPaul Okunieff .

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Editors and Affiliations

  1. , Department of Physiology & Biophysics, Case Western Reserve University School o, 10900 Euclid Avenue, Cleveland, 44106, Ohio, USA
    Joseph C. LaManna
  2. , Department of Nutrition, Case Western Reserve University, Cleveland, 44106, Ohio, USA
    Michelle A. Puchowicz
  3. , Department of Neurology, Case Western Reserve University, Cleveland, 44106, Ohio, USA
    Kui Xu
  4. , Institute of Cellular Medicine, Newcastle University, Newcastle upon Tyne, NE1 7RU, United Kingdom
    David K Harrison
  5. Synthesizer, Inc., Westminster Rd 2773, Ellicott City, 21043, Maryland, USA
    Duane F. Bruley

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Zhang, M. et al. (2011). Antioxidant Properties of Quercetin. In: LaManna, J., Puchowicz, M., Xu, K., Harrison, D., Bruley, D. (eds) Oxygen Transport to Tissue XXXII. Advances in Experimental Medicine and Biology, vol 701. Springer, Boston, MA. https://doi.org/10.1007/978-1-4419-7756-4\_38

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