Protein Detection by Simple Western™ Analysis (original) (raw)
Abstract
Protein Simple© has taken a well-known protein detection method, the western blot, and revolutionized it. The Simple Western™ system uses capillary electrophoresis to identify and quantitate a protein of interest. Protein Simple© provides multiple detection apparatuses (Wes, Sally Sue, or Peggy Sue) that are suggested to save scientists valuable time by allowing the researcher to prepare the protein sample, load it along with necessary antibodies and substrates, and walk away. Within 3–5 h the protein will be separated by size, or charge, immuno-detection of target protein will be accurately quantitated, and results will be immediately made available. Using the Peggy Sue instrument, one study recently examined changes in MAPK signaling proteins in the sex-determining stage of gonadal development. Here the methodology is described.
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References
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Authors and Affiliations
- Department of Pathology, University of Oklahoma Health Science Center, 940 S.L. Young Blvd., BMSB 415, Oklahoma City, OK, 73104, USA
Valerie M. Harris
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- Valerie M. Harris
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Correspondence toValerie M. Harris .
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- Department of Medicine, University of Oklahoma, Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of Veterans, Oklahoma City, Oklahoma, USA
Biji T. Kurien - Department of Medicine, Arthritis and Clinical Immunology, Oklahoma Medical Research Foundation, Health Sciences Center, Department of Veterans Affairs Medical Center, Oklahoma City, Oklahoma, USA
R. Hal Scofield
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© 2015 Springer Science+Business Media New York
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Harris, V.M. (2015). Protein Detection by Simple Western™ Analysis. In: Kurien, B., Scofield, R. (eds) Western Blotting. Methods in Molecular Biology, vol 1312. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2694-7\_47
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- DOI: https://doi.org/10.1007/978-1-4939-2694-7\_47
- Publisher Name: Humana Press, New York, NY
- Print ISBN: 978-1-4939-2693-0
- Online ISBN: 978-1-4939-2694-7
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