Protocols for In Vitro Propagation, Conservation, Synthetic Seed Production, Embryo Rescue, Microrhizome Production, Molecular Profiling, and Genetic Transformation in Ginger (Zingiber officinale Roscoe.) (original) (raw)

Abstract

Ginger is a rhizomatous plant that belongs to the family Zingiberaceae. It is a herbaceous perennial but cultivated as annual, with crop duration of 7–10 months. Ginger is native to India and Tropical South Asia. The tuberous rhizomes or underground stems of ginger are used as condiment, an aromatic stimulant, and food preservative as well as in traditional medicine. Ginger is propagated vegetatively with rhizome bits as seed material. Cultivation of ginger is plagued by rhizome rot diseases, most of which are mainly spread through infected seed rhizomes. Micropropagation will help in production of disease-free planting material. Sexual reproduction is absent in ginger, making recombinant breeding very impossible. In vitro technology can thus become the preferred choice as it can be utilized for multiplication, conservation of genetic resources, generating variability, gene transfer, molecular tagging, and their utility in crop improvement of these crops.

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Abbreviations

BA:

Benzyl adenine

IBA :

Indole-3-butyric acid

Kin:

Kinetin

NAA :

α-Naphthalene acetic acid

MS:

Murashige and Skoog

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Authors and Affiliations

  1. All India Coordinated Research Project on Spices, Indian Institute of Spices Research, Kozhikode, 673 012, Kerala, India
    K. Nirmal Babu, V. Sumathi, K. Praveen & P. N. Ravindran
  2. Central Plantation Crops Research Institute, Kasaragod, 671124, Kerala, India
    K. Samsudeen
  3. Providence Women’s College, Kozhikode, 673 009, India
    Minoo Divakaran
  4. Centre for Medicinal Plants Research, Arya Vaidya Sala, Kottakkal, Kerala, India
    Geetha S. Pillai
  5. World Noni Research Foundation, Chennai, 600 020, India
    K. V. Peter

Authors

  1. K. Nirmal Babu
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  2. K. Samsudeen
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  3. Minoo Divakaran
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  4. Geetha S. Pillai
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  5. V. Sumathi
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  6. K. Praveen
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  7. P. N. Ravindran
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  8. K. V. Peter
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Corresponding author

Correspondence toK. Nirmal Babu .

Editor information

Editors and Affiliations

  1. Applied Biology, Univ of Helsinki, Helsinki, Finland
    S.Mohan Jain

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Nirmal Babu, K. et al. (2016). Protocols for In Vitro Propagation, Conservation, Synthetic Seed Production, Embryo Rescue, Microrhizome Production, Molecular Profiling, and Genetic Transformation in Ginger (Zingiber officinale Roscoe.). In: Jain, S. (eds) Protocols for In Vitro Cultures and Secondary Metabolite Analysis of Aromatic and Medicinal Plants, Second Edition. Methods in Molecular Biology, vol 1391. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3332-7\_28

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