Genome-Wide Determination of DNA Methylation by Hpa II Tiny Fragment Enrichment by Ligation-Mediated PCR (HELP) for the Study of Acute Leukemias (original) (raw)

Summary

Aberrant distribution of cytosine methylation in cancer has been linked to deregulation of gene expression and genomic instability. DNA methylation changes in cancer include both hyper and hypomethylation, and the precise localization of these changes is directly related to the impact they have on gene regulation. To determine both the localization and extent of DNA methylation status under different conditions, we have developed the _Hpa_II tiny fragment enrichment by ligation-mediated PCR (HELP) assay, a microarray-based technique that allows the simultaneous interrogation of the methylation status of hundreds of thousands of CpG dinucleotides. The HELP assay allows methylation levels throughout the genome to be accurately determined so that the epigenetic state of leukemia cells can be identified, compared, and contrasted.

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Authors and Affiliations

  1. Department of Medicine and Department of Molecular Genetics, Albert Einstein College of Medicine, 1300 Morris Park Ave., Ullmann 911, Bronx, NY, 10461, USA
    Maria E. Figueroa, Ari Melnick & John M. Greally

Authors

  1. Maria E. Figueroa
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  2. Ari Melnick
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  3. John M. Greally
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Corresponding author

Correspondence toJohn M. Greally .

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Editors and Affiliations

  1. King's College London, The University of London, London, UK
    Chi Wai Eric So (Professor and Chair in Leukaemia Biology) (Professor and Chair in Leukaemia Biology)

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© 2009 Humana Press, a part of Springer Science+Business Media, LLC

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Figueroa, M.E., Melnick, A., Greally, J.M. (2009). Genome-Wide Determination of DNA Methylation by Hpa II Tiny Fragment Enrichment by Ligation-Mediated PCR (HELP) for the Study of Acute Leukemias. In: Eric So, C.W. (eds) Leukemia. Methods in Molecular Biology™, vol 538. Humana Press. https://doi.org/10.1007/978-1-59745-418-6\_20

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