Glycan Microarrays (original) (raw)

Abstract

Glycan microarrays are presentations of multiple glycans or glycoconjugates printed on a single slide for screening with glycan-binding proteins (GBPs), which include lectins, antibodies, bacteria, and viruses. Glycans derivatized with functional groups can be immobilized onto appropriately activated glass slides to generate glycan microarrays where each glycan is printed at similar concentrations. Here we describe a method for fluorescently and functionally derivatizing free reducing glycans, printing microarrays, and interrogating the microarrays with GBPs.

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Authors and Affiliations

  1. Department of Biochemistry and The Glycomics Center, Emory University School of Medicine, Atlanta, GA, USA
    Xuezheng Song, Jamie Heimburg-Molinaro, David F. Smith & Richard D. Cummings

Authors

  1. Xuezheng Song
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  2. Jamie Heimburg-Molinaro
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  3. David F. Smith
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  4. Richard D. Cummings
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Corresponding author

Correspondence toRichard D. Cummings .

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Editors and Affiliations

  1. Royston, SG8 0ZN, Hertfordshire, United Kingdom
    Edward D. Zanders

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© 2012 Springer Science+Business Media, LLC

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Song, X., Heimburg-Molinaro, J., Smith, D.F., Cummings, R.D. (2012). Glycan Microarrays. In: Zanders, E. (eds) Chemical Genomics and Proteomics. Methods in Molecular Biology, vol 800. Humana Press. https://doi.org/10.1007/978-1-61779-349-3\_11

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