Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence Imaging (original) (raw)

Abstract

We describe simple and sensitive in vitro and in vivo assays to analyze Plasmodium liver stage development using transgenic P. berghei parasites (_Pb_GFP-Luccon), which express the bioluminescent reporter protein, luciferase. In these assays, parasite development in hepatocytes is visualized and quantified by real-time bioluminescence imaging both in culture and in live mice. We also describe quantification of in vitro liver-stage development by measuring luminescence using a microplate reader. Reporter-parasite based quantification of liver-stage development is faster and correlates very well with established quantitative RT-PCR methods currently used to assess parasite development inside hepatocytes, both in live mice and in culture.

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Acknowledgment

TA, SC, and SMK received financial support of Top Institute Pharma (The Netherlands) project: T4-102.

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Authors and Affiliations

  1. Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    Takeshi Annoura, Séverine Chevalley, Chris J. Janse, Blandine Franke-Fayard & Shahid M. Khan

Authors

  1. Takeshi Annoura
  2. Séverine Chevalley
  3. Chris J. Janse
  4. Blandine Franke-Fayard
  5. Shahid M. Khan

Corresponding author

Correspondence toChris J. Janse.

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Editors and Affiliations

  1. , Malaria Biology and Genetics Unit, Institut Pasteur, Rue du Dr Roux 28, Paris, 75724, France
    Robert Ménard

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© 2012 Springer Science+Business Media, LLC

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Annoura, T., Chevalley, S., Janse, C.J., Franke-Fayard, B., Khan, S.M. (2012). Quantitative Analysis of Plasmodium berghei Liver Stages by Bioluminescence Imaging. In: Ménard, R. (eds) Malaria. Methods in Molecular Biology, vol 923. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-026-7\_30

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