Screening Inhibitors of P. berghei Blood Stages Using Bioluminescent Reporter Parasites (original) (raw)

Abstract

We describe two improved assays for in vitro and in vivo screening of inhibitors and chemicals for antimalarial activity against blood stages of the rodent malaria parasite, Plasmodium berghei. These assays are based on the determination of bioluminescence in small blood samples that is produced by reporter parasites expressing luciferase. Luciferase production increases as the parasite develops in a red blood cell and as the numbers of parasites increase during an infection. In the first assay, in vitro drug luminescence (ITDL) assay, the in vitro development of ring-stage parasites into mature schizonts in the presence and absence of candidate inhibitor(s) is quantified by measuring luciferase activity after the parasites have been allowed to mature into schizonts in culture. In the second assay, the in vivo drug luminescence (IVDL) assay, in vivo parasite growth (using a standard 4-day suppressive drug test) is quantified by measuring the luciferase activity of circulating parasites in samples of tail blood of drug-treated mice.

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Acknowledgment

Jing-wen Lin was supported by the China Scholarship Council (CSC).

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Authors and Affiliations

  1. Center of Infectious Diseases, Leiden University Medical Center, Leiden, The Netherlands
    Jing-wen Lin, Mohammed Sajid, Jai Ramesar, Shahid M. Khan, Chris J. Janse & Blandine Franke-Fayard

Authors

  1. Jing-wen Lin
  2. Mohammed Sajid
  3. Jai Ramesar
  4. Shahid M. Khan
  5. Chris J. Janse
  6. Blandine Franke-Fayard

Corresponding author

Correspondence toChris J. Janse.

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Editors and Affiliations

  1. , Malaria Biology and Genetics Unit, Institut Pasteur, Rue du Dr Roux 28, Paris, 75724, France
    Robert Ménard

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© 2012 Springer Science+Business Media, LLC

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Lin, Jw., Sajid, M., Ramesar, J., Khan, S.M., Janse, C.J., Franke-Fayard, B. (2012). Screening Inhibitors of P. berghei Blood Stages Using Bioluminescent Reporter Parasites. In: Ménard, R. (eds) Malaria. Methods in Molecular Biology, vol 923. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-026-7\_35

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