Automation of Data Acquisition in Electron Crystallography (original) (raw)

Abstract

General considerations for using automation software for acquiring high-resolution images of 2D crystals under low-dose conditions are presented. Protocol modifications specific to this application in Leginon are provided.

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Acknowledgments

This work is supported by National Institutes of Health (NIH) through the National Center for Research Resources (NCRR) P41 program (Grants RR17573 and RR023093). I would like to thank Bridget Carragher for valuable comments and editing of the manuscript.

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Authors and Affiliations

  1. Department of Cell Biology, The National Resource for Automated Molecular Microscopy, The Scripps Research Institute, La Jolla, CA, USA
    Anchi Cheng

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  1. Anchi Cheng
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Correspondence toAnchi Cheng .

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Editors and Affiliations

  1. School of Biology, Georgia Institute of Technology, Ferst Dr. 310, Atlanta, 30332, Georgia, USA
    Ingeborg Schmidt-Krey
  2. , Dept. Biochemistry & Biophysics, University of California, San Francisco, 16th St. 600, San Francisco, 94158, California, USA
    Yifan Cheng

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Cheng, A. (2013). Automation of Data Acquisition in Electron Crystallography. In: Schmidt-Krey, I., Cheng, Y. (eds) Electron Crystallography of Soluble and Membrane Proteins. Methods in Molecular Biology, vol 955. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-176-9\_17

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