Estimating blood-brain barrier opening in a rat model of hemorrhagic transformation with Patlak plots of Gd-DTPA contrast-enhanced MRI (original) (raw)

Abstract

Patlak plot processing [2, 3] of Gd-shifted T1 relaxation-time images from a rat model of hemorrhagic transformation yielded estimates and maps of the blood-to-brain influx rate constant of Gd-DTPA (K1). The Patlak plots also produced a heretofore unrecognized parameter, the distribution space of the intravascularGd-shifted protons (Vp), an index of blood-to-tissue transfer of water. The K1 values for Gd-DTPA were very high for the regions of blood-brain barrier (BBB) opening and were similar to those of 14C-sucrose concurrently obtained by quantitative autoradiographic (QAR) analysis. In these same ROI’s, Vp was five-fold greater than normal, which suggests that the permeability of the BBB to water was also increased. The 14C-sucrose space of distribution in the ischemic ROI’s was around 8%, thus indicating a sizable interstitial space. The spatial resolving power of Gd-DTPA-ΔT1 imaging was rather good, although no match for 14C-sucrose-QAR. This study shows that quantitative ΔT1-MRI estimates of regional blood-brain transfer constants of Gd-DTPA and water distribution are possible when Patlak plots are employed to process the data. This approach may be useful for tracking the time-course of BBB barrier function in both animals and humans.

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References

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Authors and Affiliations

  1. Department of Anesthesiology, Henry Ford Health Sciences Center, Detroit, MI, USA
    Dr. Joseph D. Fenstermacher & T. Nagaraja
  2. Department of Neurology, Henry Ford Health Sciences Center, Detroit, MI, USA
    R. A. Knight, J. R. Ewing, V. Nagesh, J. S. Yee & P. A. Arniego
  3. Department of Physics, Oakland University, Rochester, MI, USA
    R. A. Knight & J. R. Ewing

Authors

  1. Dr. Joseph D. Fenstermacher
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  2. R. A. Knight
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  3. J. R. Ewing
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  4. T. Nagaraja
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  5. V. Nagesh
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  6. J. S. Yee
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  7. P. A. Arniego
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Corresponding author

Correspondence toJoseph D. Fenstermacher .

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Editors and Affiliations

  1. Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan
    T. Kuroiwa
  2. Institute für Chirurgische Forschung, Ludwig-Maximilians-Universität, München, Germany
    A. Baethmann
  3. Department of Neurosurgery, Polish Academy of Scienes, Warsaw, Poland
    Z. Czernicki
  4. Section of Neurosurgery, University of Michigan, Ann Arbor, USA
    J. T. Hoff
  5. Department of Neurosurgery, Musashino Red Cross Hospital, Tokyo, Japan
    U. Ito
  6. Department of Neurological Surgery, Nihon University, Tokyo, Japan
    Y. Katayama
  7. Medical College of Virgina, Richmond, USA
    A. Marmarou
  8. Department of Neurosurgery, Newcastle Gerneral Hospital, Newcastle-upon-Tyne, UK
    B. A. D. Mendelow
  9. Neurochirurgische Universitätklinik, Ludwig-Maximilians-Universität, München, Germany
    H.-J. Reulen

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© 2003 Springer-Verlag Wien

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Fenstermacher, J.D. et al. (2003). Estimating blood-brain barrier opening in a rat model of hemorrhagic transformation with Patlak plots of Gd-DTPA contrast-enhanced MRI. In: Kuroiwa, T., et al. Brain Edema XII. Acta Neurochirurgica Supplements, vol 86. Springer, Vienna. https://doi.org/10.1007/978-3-7091-0651-8\_7

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