DNA vaccines against rotavirus infections (original) (raw)

Summary

Plasmid DNA vaccines encoding for murine rotaviral proteins VP4, VP6, and VP7 were tested in adult BALB/c mice for their ability to induce immune responses and provide protection against rotavirus challenge. Serum antibodies were measured by virus neutralization and by ELISA. Cellular immunity was assessed by measuring cytotoxic T cell (CTL) responses. The vaccines were administered by inoculation into cells of the epidermis with an Accell gene gun (Auragen, Inc., Middleton, WI, USA). Each of the three vaccines elicited rotavirus-specific serum antibodies as measured by ELISA. Virus neutralizing antibodies were detected in mice receiving DNA vaccines encoding for VP4 and VP7, but not in those which received the plasmid encoding for VP6. Vaccines encoding for VP4, VP6, or VP7 generated virus-specific CTL responses in recipient mice. Efficacy of the vaccines was determined by challenge with homotypic rotaviruses. Each of the three vaccines was effective in protecting mice against infection after rotavirus (100 ID50) challenge. Significant reductions (p < 0.0002, analysis of variance) in viral excretion measured over a 9 day period were seen in mice receiving the DNA vaccines compared with mice that received control plasmids.

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References

  1. Bishop RF (1993) Development of candidate rotavirus vaccines. Vaccine 11: 247–254
    Article PubMed CAS Google Scholar
  2. Both GW, Lockett LJ, Janardhana V, Edwards SJ, Bellamy AR, Graham FL, Prevec L, Andrew ME (1993) Protective immunity to rotavirus-induced diarrhea is passively transferred to newborn mice from naive dams vaccinated with a single dose of a recombinant adenovirus expressing rotavirus VP7sc. Virology 193: 940–950
    Article PubMed CAS Google Scholar
  3. Conner ME, Crawford SE, Barone C, Estes MK (1993) Rotavirus vaccine administered parenterally induces protective immunity. J Virol 67: 6633–6641
    PubMed CAS Google Scholar
  4. Conner ME, Matson DO, Estes MK (1994) Rotavirus vaccines and vaccination potential. In: Ramig RF (ed) Rotaviruses. Current Topics in Microbiology and Immunology 185: 285–371
    Google Scholar
  5. Dharakul T, Labbe M, Cohen J, Bellamy AR, Street JE, Mackow ER, Fiore L, Rott L, Greenberg HB (1991) Immunization with baculovirus-expressed recombinant rotavirus proteins VP1, VP4, VP6 and VP7 induce CD8 + T lymphocytes that mediate clearance of chronic rotavirus infection in SCID mice. J Virol 65: 5928–5932
    PubMed CAS Google Scholar
  6. Dormitzer P, Burns JW, Greenberg HB, Prevec L, Graham F, Boyle DB, Both G (1993) Vaccination of adult mice with recombinant viruses expressing a protective rotavirus VP7sc. Abstr. IXth Intl. Cong. Virology, W21-2, p43
    Google Scholar
  7. Estes MR, Cohen J (1989) Rotavirus gene structure and function. Microbiol Rev 53: 410–449
    PubMed CAS Google Scholar
  8. Feng N, Burns JW, Bracy L, Greenberg HB (1994) Comparison of mucosal and systemic humoral immune responses and subsequent protection in mice orally inoculated with a homologous or a heterologous rotavirus. J Virol 68: 7766–7733
    PubMed CAS Google Scholar
  9. Fynan EF, Webster RG, Fuller DH, Haynes JR, Santoro JC, Robinson HL (1993) DNA vaccines: protective immunizations by parenteral, mucosal, and gene-gun inoculations. Proc Natl Acad Sci USA 90: 11478–11482
    Article PubMed CAS Google Scholar
  10. Burns JW, Pajouh MS, Krishnaney AA, Greenberg HB (1996) Protective effect of rotavirus VP6-specific IgA monoclonal antibodies that lack neutralizing activity. Science 272: 104–107
    Article PubMed CAS Google Scholar
  11. Herrmann JE, Blacklow NR, Perron DM, Cukor G, Krause PJ, Hyams JS, Barrett HJ, Ogra PL (1985) Enzyme immunoassay with monoclonal antibodies for the detection of rotavirus in stool specimens. J Infect Dis 152: 831–832
    Article Google Scholar
  12. Herrmann JE, West K, Bruns M, Ennis FA (1990) Effect of rimantadine on cytotoxic T lymphocyte responses and immunity to reinfection in mice infected with influenza A virus. J Infect Dis 161: 180–184
    Article PubMed CAS Google Scholar
  13. Kapikian AZ, Flores J, Midthun K, Hoshino Y, Green KY, Gorziglia M, Nishikawa K, Chanock RM, Potash L, Perez-Schael I (1989) Strategies for the development of a rotavirus vaccine against infantile diarrhea with an update on clinical trials of rotavirus vaccine. Adv Exp Med Biol 257: 67–89
    Article PubMed CAS Google Scholar
  14. Mazenec MB, Kaetzel CS, Lamm ME, Fletcher D, Nedrud JG (1992) Intracellular neutralization of virus by immunoglobulin A antibodies. Proc Natl Acad Sci USA 89: 6901–6905
    Article Google Scholar
  15. McNeal MM, Sheridan JR, Ward RL (1992) Active protection against rotavirus infection of mice following intraperitoneal immunization. Virology 191: 150–157
    Article PubMed CAS Google Scholar
  16. Offit PA, Dudzik KI (1988) Rotavirus-specific cytotoxic T lymphocytes cross-react with target cells infected with different rotavirus serotypes. J Virol 62: 127–131
    PubMed CAS Google Scholar
  17. Riepenhoff-Talty M, Dharakul R, Kowalski E, Sterman D, Ogra PL (1987) Rotavirus infection in mice: pathogenesis and immunity. Adv Exp Med Biol 216B: 1015–1023
    Google Scholar
  18. Saif LJ, Jackwood DJ (1990) Enteric Virus Vaccines: Theoretical considerations, current status, and future approaches. In: Saif LJ, Theil KW (eds) Viral Diarrheal of Man and Animals. CRC Press, Boca Raton, pp 313–329
    Google Scholar
  19. Ulmer JB, Donnelly JJ, Parker SE, Rhodes GH, Feigner PI, Dwarki VJ, Gromkowski SH, Deck RR, DeWitt CM, Friedman A, Hawe LA, Leander KR, Martinez D, Perry HC, Shiver JW, Montgomery DL, Liu MA (1993) Heterologous protection against influenza by injection of DNA encoding a viral protein. Science 259: 1745–1749
    Article PubMed CAS Google Scholar
  20. Ward RL, McNeal MM, Sheridan JF (1990) Development of an adult mouse model for studies on protection against rotavirus. J Virol 64: 5070–5075
    PubMed CAS Google Scholar
  21. Webster RG, Fynan EF, Santoro JC, Robinson H (1994) Protection of ferrets against influenza challenge with a DNA vaccine to the haemagglutinin. Vaccine 12: 1495–1498
    Article PubMed CAS Google Scholar
  22. Xiang ZO, Spitalnik S, Tran M, Wunner W, Cheng J, Ertl HCL (1994) Vaccination with a plasmid vector carrying the rabies virus glycoprotein gene induces protective immunity against rabies virus. Virology 199: 132–140
    Article PubMed CAS Google Scholar
  23. Zarozinski CC, Fynan EF, Selin LK, Robinson HL, Welsh RM (1995) Protective CTL-dependent immunity and enhanced immunopathology in mice immunized by particle bombardment with DNA encoding an internal protein. J Immunol 154: 4010–4017
    PubMed CAS Google Scholar

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Authors and Affiliations

  1. Division of Infectious Diseases and Immunology, University of Massachusetts Medical Center, 55 Lake Avenue North, Worcester, MA, 01655, USA
    J. E. Herrmann, S. C. Chen & E. F. Fynan
  2. Department of Pathology, University of Massachusetts Medical School, Worcester, Massachusetts, USA
    J. C. Santoro & H. L. Robinson
  3. Department of Biology, Worcester State College, Worcester, Massachusetts, USA
    E. F. Fynan
  4. Division of Gastroenterology, Stanford University School of Medicine, Stanford, California, USA
    H. B. Greenberg

Authors

  1. Dr. J. E. Herrmann
  2. S. C. Chen
  3. E. F. Fynan
  4. J. C. Santoro
  5. H. B. Greenberg
  6. H. L. Robinson

Editor information

Editors and Affiliations

  1. Sapporo Medical University, Sapporo, Japan
    Shunzo Chiba & Shuji Nakata &
  2. Baylor College of Medicine, Houston, USA
    Mary K. Estes
  3. Colorado State University, Fort Collins, USA
    Charles H. Calisher

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© 1996 Springer-Verlag Wien

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Herrmann, J.E., Chen, S.C., Fynan, E.F., Santoro, J.C., Greenberg, H.B., Robinson, H.L. (1996). DNA vaccines against rotavirus infections. In: Chiba, S., Estes, M.K., Nakata, S., Calisher, C.H. (eds) Viral Gastroenteritis. Archives of Virology, vol 12. Springer, Vienna. https://doi.org/10.1007/978-3-7091-6553-9\_22

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