Immunolocalization of HP1 proteins in metaphasic mammalian chromosomes (original) (raw)
Abstract
HP1 proteins are conserved non histone chromosomal proteins involved in the epigenetic repression of transcription. Three HP1 proteins, HP1α, HP1β and HP1γ are expressed in mammalian cells. Polyclonal antibodies directed against peptides specific for HP1α and HP1γ were elicited in rabbits, affinity purified, then used to localize both proteins on spreads of unfixed metaphasic chromosomes. We show here, by conventional and confocal microscopy, that both proteins are localized at centromeres and pericentromeres, and that HP1γ is also present on euchromatic sites of chromosome arms.
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- Courvalin JC, Hernandez-Verdun D, Gosti-Testu F, Marty MC, Maunoury R, Bornens M (1986). A protein of Mr 80,000 is associated with the nucleolus organizer of human cell lines. Chromosoma 94: 353–361.
Article PubMed CAS Google Scholar - Eissenberg JC, James TC, Foster-Hartnett DM, Hartnett T, Ngan V, Elgin SC (1990). Mutation in a heterochromatin-specific chromosomal protein is associated with suppression of position-effect variegation in Drosophila melanogaster. Proc Natl Acad Sci USA 87: 9923–9927.
Article PubMed CAS Google Scholar - Furuta K, Chan EKL, Kiyosawa K, Reimer G, Luderschmidt C, Tan EM (1997). Heterochromatin protein HPlHsβ (p25ß) and its localization with centromeres in mitosis. Chromosoma 106: 11–19.
Article PubMed CAS Google Scholar - Gasser SM, Laemmli UK (1987). Improved methods for the isolation of individual and clustered mitotic chromosomes. Exp Cell Res 173: 85–98.
Article PubMed CAS Google Scholar - Horsley D, Hutchings A, Butcher GW, Singh PB (1996). M32, a murine homologue of Drosophila heterochromatin protein 1 (HP1), localises to euchro-matin within interphase nuclei and is largely excluded from constitutive heterochromatin. Cytogenet Cell Genet 73: 308–311.
Article PubMed CAS Google Scholar - James TC, Elgin SC (1986). Identification of a non histone chromosomal protein associated with heterochromatin in Drosophila Melanogaster and its gene. Mol Cell Biol 6: 3862–3872.
PubMed CAS Google Scholar - James TC, Eissenberg JC, Craig C, Dietrich V, Hobson A, Elgin SC (1989). Distribution patterns of HP1, a heterochromatin-associated nonhistone chromosomal protein of Drosophila. Eur J Cell Biol 50: 170–180.
PubMed CAS Google Scholar - Jeppesen P, Mitchell A, Turner B, Perry P (1992). Antibodies to defined histone epitopes reveal variations in chromatin conformation and underacetylation of centric heterochromatin in human metaphase chromosomes. Chromosoma 101: 322–332.
Article PubMed CAS Google Scholar - Minc E, Allory Y, Worman HJ, Courvalin J-C, Buendia B (1999). Localization and phosphorylation of HP1 proteins during the cell cycle in mammalian cells. Chromosoma 108: 220–234.
Article PubMed CAS Google Scholar - Minc E, Courvalin J-C, Buendia B (2000). HPlγ associates with euchromatin and heterochromatin in mammalian nuclei and chromosomes. Cytogenet Cell Genet 90: 279–284.
Article PubMed CAS Google Scholar - Moroi Y, Peebles C, Fritzler MJ, Steigerwald J, Tan EM (1980). Autoantibody to centromere (kinetochore) in scleroderma sera. Proc Natl Acad Sci USA 77: 1627–1631.
Article PubMed CAS Google Scholar - Nicol L, Jeppesen P (1994). Human autoimmune sera recognize a conserved 26 kD protein associated with mammalian heterochromatin that is homologous to heterochromatin protein 1 of Drosophila. Chromosome Res 2: 245–253
Article PubMed CAS Google Scholar - Saunders WS, Chue C, Goebl M, Craig C, Clark RF, Powers JA, Eissenberg JC, Elgin SCR, Rothfield NF, Earnshaw WC (1993). Molecular cloning of a human homologue of Drosophila heterochromatin protein HP1 using anti centromere autoantibodies with antichromo specificity. J Cell Sci 104:573–582.
PubMed Google Scholar - Wreggett KA, Hill F, James PS, Hutchings A, Butcher GW, Singh PB (1994). A mammalian homologue of Drosophila heterochromatin protein 1 (HP1) is a component of constitutive heterochromatin. Cytogenet Cell Genet 66: 99–103.
Article PubMed CAS Google Scholar
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- Département de Biologie Cellulaire, Institut Jacques Monod, CNRS, Universités Paris 6 et 7, Paris, France
E. Minc, Y. Allory, J.-C. Courvalin & Brigitte Buendia
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- E. Minc
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Correspondence toBrigitte Buendia .
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- Centre for Advanced Study in Cell and Chromosome Research, Department of Botany, University of Calcultta, Calcultta, India
Arun Kumar Sharma & Archana Sharma &
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© 2001 Springer Science+Business Media Dordrecht
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Minc, E., Allory, Y., Courvalin, JC., Buendia, B. (2001). Immunolocalization of HP1 proteins in metaphasic mammalian chromosomes. In: Sharma, A.K., Sharma, A. (eds) Chromosome Painting. Springer, Dordrecht. https://doi.org/10.1007/978-94-010-0330-8\_18
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- DOI: https://doi.org/10.1007/978-94-010-0330-8\_18
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