The plant mitochondrial open reading frame orf221 encodes a membrane-bound protein (original) (raw)

Abstract

We have shown that the open reading frame orf221 is an active mitochondrial gene which encodes a novel mitochondrial polypeptide. The orf221 sequence is common to higher plants but absent in animal and fungal mitochondria. A mitochondrial polypeptide with an apparent molecular weight of 21 000 was detected with a polyclonal antibody raised against an ORF221 fusion protein. In organello translation followed by immunoprecipitation with the anti-ORF221 antibody demonstrated that this polypeptide is encoded by the orf221 gene in plant mitochondria. The ORF221 was found to be a mitochondrial membrane protein in normal (N), cms-T, and cms-C cytoplasms of several inbred lines of maize (Zea mays L.) and in other plant species.

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Authors and Affiliations

  1. Departamento de Genética e Evolução, Instituto de Biologia, Universidade Estadual de Campinas (UNICAMP), Cx. Postal 6109, CEP13081, Campinas, SP, Brasil
    Laudenir M. Prioli
  2. Department of Genetics, North Carolina State University, Box 7614, 27695-7614, Raleigh, NC, USA
    Jintai Huang & Charles S. Levings III

Authors

  1. Laudenir M. Prioli
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  2. Jintai Huang
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  3. Charles S. Levings III
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Prioli, L.M., Huang, J. & Levings, C.S. The plant mitochondrial open reading frame orf221 encodes a membrane-bound protein.Plant Mol Biol 23, 287–295 (1993). https://doi.org/10.1007/BF00029005

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