Purification, spectroscopic analysis and biological activity of the macrocyclic dihydroxamate siderophore alcaligin produced by Bordetella pertussis and Bordetella bronchiseptica (original) (raw)
Abstract
Hydroxamate siderophores of virulent Bordetella pertussis and Bordetella bronchiseptica strains were purified using a simple large-scale isolation procedure, and identified by various spectroscopic techniques as the macrocyclic dihydroxamate siderophore trivially known as alcaligin, 1,8(S),11,18(S)-tetrahydroxy-1,6,11,16-tetraazacycloeicosane-2,5,12,15-tetrone, which was previously isolated from the taxonomically-related bacterial species Alcaligenes denitrificans subsp. xylosoxydans. Alcaligin purified from iron-depleted cultures of B. pertussis and B. bronchiseptica exhibited specific growth-promoting activity under iron-restricted conditions for Bordetella indicator strains, and were active in [55Fe]ferric alcaligin transport assays. Evidence suggests that several C 2-symmetric conformations of alcaligin exist simultaneously in both methanolic and aqueous solution.
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Authors and Affiliations
- Department of Microbiology and Immunology, East Carolina University School of Medicine, Greenville, NC, USA
Timothy J. Brickman, Jan-Gerd Hansel, Marvin J. Miller & Sandra K. Armstrong - Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN, USA
Jan-Gerd Hansel & Marvin J. Miller
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- Timothy J. Brickman
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Brickman, T.J., Hansel, JG., Miller, M.J. et al. Purification, spectroscopic analysis and biological activity of the macrocyclic dihydroxamate siderophore alcaligin produced by Bordetella pertussis and Bordetella bronchiseptica.Biometals 9, 191–203 (1996). https://doi.org/10.1007/BF00144625
- Received: 28 June 1995
- Accepted: 15 July 1995
- Issue Date: April 1996
- DOI: https://doi.org/10.1007/BF00144625