Calculation of sequence divergence from the thermal stability of DNA heteroduplexes (original) (raw)

Summary

Measurements are reported of the thermal stability of DNA heteroduplexes between clones of the eta-globin pseudogene from a variety of primates. The known sequences of this 7.1-kb region differ from each over a range from 1.6% for human versus chimp to nearly 12% for human versus spider monkey. Thermal stability was determined by standard hydroxyapatite thermal elution, and the results show a precisely linear decrease in thermal stability with divergence. The slope of the regression line is 1.18% sequence divergence per degree centigrade reduction in thermal stability.

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1. Mark S. Springer
   Present address: Department of Biology, University of California, 92521, Riverside, CA, USA

Authors and Affiliations

1. Kerckhoff Marine Laboratory, California Institute of Technology, 101 Dahlia, 92625, Corona del Mar, CA, USA
   Mark S. Springer & Roy J. Britten
2. Division of Biology, California Institute of Technology, 91125, Pasadena, CA, USA
   Eric H. Davidson

Authors

1. Mark S. Springer
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2. Eric H. Davidson
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3. Roy J. Britten
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Springer, M.S., Davidson, E.H. & Britten, R.J. Calculation of sequence divergence from the thermal stability of DNA heteroduplexes.J Mol Evol 34, 379–382 (1992). https://doi.org/10.1007/BF00162994

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• Received: 30 September 1991
• Accepted: 03 December 1991
• Issue Date: May 1992
• DOI: https://doi.org/10.1007/BF00162994

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